Affinity chromatography
Immobilized nucleotides for affinity chromatography
One step purification of nucleotide binding proteins
NTP binding
protein
Matrix
Affinity column
Sepharose ®
4B or
Toyopearl AF-650M
Protein Image: Antony M. Dean, University of Minnesota:
www.cbs.umn.edu/labs/deanlab/
® Sepharose is a trademark of Amersham Biosciences, Ltd.
Linker
Several types
and lengths
available
Nucleotide
Immobilized at different
positions for optimal fit
in nucleotide binding
pocket
Affinity chromatography
Tailor-made affinity chromatography from Jena Bioscience
Immobilized ligands (selection) …
…for your research
M a tr i x
6-linked ATPs
L in k e r
NH
O
O
P
HO
P
O
O
N
O
P
O
O
N
O
O
O
OH
M a tr ix
N
N
OH
L in ke r
O
P
N
O
P
O
O
Base
O
P
O
O
H
OH
• Different types and lengths of linkers
• Different types of chromatography
material ranging from bulk material to premade colums that fit any machine
O
O
Jena Bioscience affinity chromatography
material is tailor-made for purification (or
for related assays) of particular NTPbinding proteins since we provide
• NTPs and dNTPs that are linked to the
matrix at various positions of sugar, base or
phosphate moiety of the nucleotide
Gamma-phosphate
linked NTPs
O
Immobilized nucleotides provide a
convenient and rapid one-step purification
procedure for a large number of proteins
such as kinases, GTPases, chaperones,
motor proteins, and others.
OH
many m o r e available at w w w . j e n a b i o s c i e n c e. c o m
Please
Please direct
direct your
your inquiries
inquiries to
to [email protected]
[email protected]
Affinity c h r o m a t o g r a p h y
Different types of chromatography material available
Pre- packed
MPLC columns
based on
Toyopearl
AF-6 5 0 M
Pre- packed
syringe columns
based on
Sepharose ® 4 B
Pre- packed
screening columns
based on
Sepharose ® 4 B
Bulk materials
based on
Sepharose ® 4 B
For
For more
more information
information please
please contact
contact us
us at
at nucleotides@jenabioscie
[email protected]
m
®
S e p h a r o s e i s a trademark of A m e r s h a m Biosciences , Ltd.
Affinity c h r o m a t o g r a p h y
Selected References
Christoforidis et al. (2000) Purification and
identification of novel Rab effectors using affinity
chromatography. Methods 20(4): 403.
Damer et al. (1998) Rapid identification of protein
phosphatase 1-binding proteins by mixed peptide
sequencing and data base searching.
Characterization of a novel holoenzymic form of
protein phosphatase 1. J. Biol. Chem.
273(38) :24396.
Haystead et al. (1993) Gamma-phosphate-linked
ATP-Sepharose for the affinity purification of
protein-kinases - rapid purification to homogeneity
of sceletal-muscle mitogen-activated proteinkinase. Eur. J. Biochem. 214 (2) :459.
Griffits et al. (1977) An examination of potential
matrices for the affinity chromatography of NADP+ linked dehydrogenases with special reference to 6phosphogluconate dehydrogenase. Biochem. J.
161(3): 561.
Product Information
Unit
Size
Price / €
N T P s immobilized on
Sepharose 4B
1 ml
50 – 2 2 0
bulk materials
5 ml
200 – 8 8 0
1 ml
80 - 240
0.2 ml
25 - 5 9
1 ml
550 - 720
Product Description
N T P s immobilized on
Sepharose 4B
pre-packed syringe
columns
N T P s immobilized on
Sepharose 4B
pre-packed screening
columns
N T P s immobilized on
pressure - stable
hydrophilic polymer
pre-packed MPLC
columns
price depends on type of nucleotide, linker position and lengths,
see www.jenabioscience.com for details