RIKEN BRC use：JCM No.
（Form M-8-GD）
Data Sheet of Living Modified Organism
Scientific Name：
Should be confirmed by：
□PCR
□others（ ）
PCR Condition Data Sheet
Please send any information such as sequence of construct or restriction map if available. Providing with the positive
control DNA (including plasmid) is highly appreciated.
reaction mixtures
DW
×Buffer
mM dNTP
primer #1
pmol/µl
Primer #2
pmol/µl
primer #3
pmol/µl
primer #4
pmol/µl
mM MgCl2
Taq polymerase (
U/µl)
DNA
total
tube 1
µl
µl
µl
µl
µl
µl
µl
µl
µl
µl
µl
tube 2
µl
µl
µl
µl
µl
µl
µl
µl
µl
µl
µl
cycling condition
min
94 ˚C
min
94 ˚C
min
˚C
min
72 ˚C
min
72 ˚C
×
cycle
Taq product name (company)
primer #1 name
(
3’
(
3’
(
3’
(
3’
sequence 5’
primer #2 name
sequence 5’
primer #3 name
sequence 5’
primer #4 name
sequence 5’
primer set
primer #1
primer #
product size
⇔
⇔
primer #
primer #
wild or mutant band
bp
bp
Construction map, electrophoretogram, and other information：
mer)
mer)
mer)
mer)
[Example]
PCR Condition Data Sheet
reaction mixtures
DW
2 ×Buffer
2.5 mM dNTP
primer 1 10 pmol/µl
Primer 2 10 pmol/µl
primer 3
primer 4
2.5 mM MgCl2
Taq polymerase ( 5 U/µl)
DNA
total
tube 1
4.8
µl
9.4
µl
2.0
µl
1.0
µl
1.0
µl
µl
µl
0.6
µl
0.2
µl
1.0
µl
20.0
µl
tube 2
µl
µl
µl
µl
µl
µl
µl
µl
µl
µl
µl
cycling condition
94 ˚C 300 min
30 min
94 ˚C
30 min
57 ˚C
72 ˚C 120 min
72 ˚C 300 min
× 34 cycle
Taq product name (company)
EX Taq (TAKARA)
primer #1 name
LCB600
sequence 5’
LCB601
sequence 5’
primer #2 name
GTTTTAAATTCCTTTAAATTT
AAAACCCCGGGGAAATTTTAA
primer #3 name
sequence 5’
primer #4 name
sequence 5’
primer set
primer #1
primer #
⇔
⇔
primer #2
primer #3
product size
120 bp
150 bp
wild or mutant band
Mutant
wild
Construction map, electrophoretogram, and other information：
( 21 mer)
3’
( 21 mer)
3’
( mer)
3’
(
mer)
3’