TOTAL ALKALINE PHOSPHATASE AND ISOENZYME
LEVELS IN PERSONS WITH Hb - GENOTYPE AA
AND SS RESPECTIVELY
CHIZEA, DANIEL CHfNEDU
/1/ljTR/[ No: 96/0241
A RESEARCH PROJECT SUBMITT~D TO THE DEPARTMENT OF
CHEMICAL SCIENCES, COLLEGE OF NATURAL SCIENCE,
UNIVERISTY OF AGRICULTURE,
ABEOKUT A, OGUN STATE
IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE
AWARD OF BACHELOR OF SCIENCE (B.Se) (CHEMISTRY /
BIOCHEMISTRY) OF THE UNIVERSITY OF AGRICULTURE,
ABEOKUT A, NIGERIA.
Abstract
The serum ALP and isoenzyme levels are studied with a view to, at least,
being able to fashion out a viable predictive means of diagnosing crisis
condition in a sickler. Alkaline phosphatases hydrolyse a number of ester
orthophosphates
at
alkaline
pH,
but
the
substrates
acted
upon
physiologically, are unknown. Sera of persons with Hb-genotype AA and
SS respectively, were obtained by centrifuging fresh whole blood. The
appropriate
concentrations
(p-nitrophenylphosphate)
of
enzyme
and
substrate
were then mixed, yielding p-nitrophenol
with
yellow color. This is. measured at 405nm with a spectrophotometre.
Findings revealed that there is a statistical difference in the level of
enzyme activity between the two genotypes.