• Increased coverage from
limited samples
• High-resolution separation
of polar analytes and
critical PTMs
• In-depth characterization
of proteoforms and
protein complexes
The Perfect Accessory for Your Mass Spectrometer
CESI 8000 Plus High Performance Separations ESI Module and Adapter Kit
CESI is a mass spectrometer accessory for the analysis of biological compounds in every analytical laboratory:
• Consumes minute sample amounts (nL volumes) with extraordinary sensitivity
• Excels at charged & polar metabolites, peptides, and more
• Enables high-resolution separation of proteins and protein-complexes
• Eliminates ion suppression so you can see what you are missing
• Robust and easy-to-use thanks to zero-dead-volume design
CESI-MS “showed better separation efficiency and
resolution with 100-fold less sample consumption
compared to an LC-based intact protein separation.”
J. Prot. Res. 2014 from the John Yates III research group,
The Scripps Research Institute, La Jolla, CA.
CESI-MS “shows a separation efficiency for highmolecular-mass compounds like larger peptides
and proteins that is remarkably higher than that
seen with any LC method”
Molecular and Cellular Proteomics 2013 from the
Herbert Lindner research group, Innsbruck, Austria
Connecting the CESI 8000 Plus to your Orbi is as easy as plug
and spray. Attach the OptiMS Adapter to your Nanospray Flex
or Flex NG and click the CESI cartridge tip into place.
Total Ion Electropherogram
Intact MAb Separation of IgG1 by CESI-MS
Reconstructed Intensity
Ion Intensity
6E+8
3E+8
3000
2500
2000
1500
1000
500
0
113
100
156
161
134
■ Phosphorylated Peptides (n=57)
85
■ Acetylated Peptides (n=688)
32
9
10
13
11
9
7
6
4
6
4
3
2
1
1
10
20
1
30
7
2
1
40
50
60
3000
1470e5
Deamidations
146389.09
146569.16
2000
1500
0
Deamidations
146701.58
146156.71
Deamidations
and oxidation
146862.70
146981.51
1460e5
1465e5
1470e5
Mass. Da
2000
146573.04
1500
146536.36
1000
500
146387.58
2500
146535.62
1000
146719.51
500
0
146189.93
1460e5
146948.99
1465e5
1470e5
1475e5
Mass. Da
Different glycosylation and modification profiles
Kindly provided by Prof. Herbert Lindner, Innsbruck Medical University, Austria.
For deep characterization of proteoforms, glycoforms, charge
states, and PTMs, CESI is the only front-end technology that
enables liquid-phase separation of proteins, metal binding,
and protein complexes. CESI-MS is an invaluable tool to
drive biopharmaceutical development as well as biochemical
mechanistic studies.
PSA Profiling Under Native Conditions
14 cells / µL, 4 cells injected
m/z
1465e5
2500
Time (Min.)
CESI-MS excels at various PTMs revealing important
biological functional variations.
1460e5
Mass. Da
■ Non-modified Peptides (n=688)
Reconstructed Intensity
Peptides Identified
0
pH 7.6: base ion electropherogram (A); density ion map of a separation
highlighting the CE migration patterns of proteoforms (B); mass spectra (C);
and corresponding deconvoluted spectra (D).
101022J000006__01_1683.d: BPC 49.0-3001.0 +All MS
1500
1000
500
Intens.
x105
10
20
30
40
50
60 Time [min]
10
20
30
40
50
60 Time [min]
0.8
0.6
0.4
0.2
0.0
CESI enables MS signal from minute amounts of sample.
CESI-MS-EMR reveals 236 proteoforms from 2.9 ng of PSA under
native conditions.
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© 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license.
RUO-MKT-07-3980-A 05/ 2016
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