Restriction Enzymes from Jena Bioscience
Enzyme
Aat I
Acc I
Acc III
Acc113 I
Acc65 I
Afa I
Afl I
Afl II
Age I
Aha III
Ahl I
Ajn I
Alu I
Alw44 I
Ama87 I
Apa I
ApaL I
AsiA I
Asp I
Asp718 I
AspS9 I
Asu II
AsuNH I
Ava I
Ava II
BamH I
Ban II
Ban III
Bbe I
Bbu I
Bcl I
Bco I
Bcu I
Bfr I
BfuC I
Bgl I
Bgl II
Bme18 I
BmeT110 I
Bmt I
Bpu14 I
BpvU I
Bsa29 I
Bse118 I
BseA I
BseB I
BseC I
BseG I
BseP I
BshF I
BshT I
BsiHKC I
BsiS I
BsoB I
Bsp106 I
Bsp119 I
Bsp120 I
Bsp13 I
Bsp143 I
Bsp19 I
Bsp68 I
BspAN I
BspC I
BspD I
BspE I
BspT I
BspT104 I
BspX I
BsrF I
BssA I
BssH I
BssH II
BssT1 I
Bst2U I
Bst98 I
BstB I
BstE II
BstEN II
BstF5 I
BstKT I
Cleavage Site 5 ➞ 3
AGG[CCT
GT[MKAC
T[CCGGA
AGT[ACT
G[GTACC
GT[AC
G[GWCC
C[TTAAG
A[CCGGT
TTT[AAA
A[CTAGT
[CCWGG
AG[CT
G[TGCAC
C[YCGRG
GGGCC[C
G[TGCAC
A[CCGGT
GACN[NNGTC
G[GTACC
G[GNCC
TT[CGAA
G[CTAGC
C[YCGRG
G[GWCC
G[GATCC
GRGCY[C
AT[CGAT
GGCGC[C
GCATG[C
T[GATCA
C[YCGRG
A[CTAGT
C[TTAAG
[GATC
GCCNNNN[NGGC
A[GATCT
G[GWCC
CY[CGRG
GCTAG[C
TT[CGAA
CGAT[CG
AT[CGAT
R[CCGGY
T[CCGGA
CC[WGG
AT[CGAT
GGATG (2/0)
G[CGCGC
GG[CC
A[CCGGT
C[YCGRG
C[CGG
C[YCGRG
AT[CGAT
TT[CGAA
G[GGCCC
T[CCGGA
[GATC
C[CATGG
TCG[CGA
GG[CC
CGAT[CG
AT[CGAT
T[CCGGA
C[TTAAG
TT[CGAA
AT[CGAT
R[CCGGY
R[CCGGY
C[TCGAG
G[CGCGC
C[CWWGG
CC[WGG
C[TTAAG
TT[CGAA
G[GTNACC
[GATC
GGATG (2/0)
GAT[C
Jena Bioscience GmbH
Loebstedter Strasse 80
D-07749 Jena
Germany
JBS Enzyme
SseB I
Acc I
BseA I
Sca I
{Kpn I}
Rsa I
Sin I
MspC I
CspA I
Dra I
Spe I
{BseB I}
Alu I
ApaL I
Ava I
Apa I
ApaL I
CspA I
Tth111 I
{Kpn I}
PspP I
Asu II
Nhe I
Ava I
Sin I
BamH I
Ban II
BseC I
{Nar I}
Sph I
Bcl I
Ava I
Spe I
MspC I
{Dpn I}, Mbo I, Sau3A I
Bgl I
Bgl II
Sin I
{Ava I}
{Nhe I}
Asu II
Pvu I
BseC I
BssA I
BseA I
BseB I
BseC I
{Fok I}
BssH II
BshF I
CspA I
Ava I
BsiS I
Ava I
BseC I
Asu II
{Apa I}
BseA I
{Dpn I}, Mbo I, Sau3A I
Nco I
Nru I
BshF I
Pvu I
BseC I
BseA I
MspC I
Asu II
BseC I
BssA I
BssA I
Sla I
BssH II
Sty I
BseB I
MspC I
Asu II
BstE II
{Dpn I}, Mbo I, Sau3A I
{Fok I}
{Dpn I}, {Mbo I}, {Sau3A I}
Enzyme
BstMA I
BstMB I
BstN I
BstO I
BstP I
BstSN I
BstX I
Bsu15 I
BsuR I
BsuTU I
BtsC I
CciN I
Cfr10 I
Cfr13 I
Cfr42 I
Cfr9 I
Cla I
Cpo I
Csp I
Csp45 I
Csp6 I
CspA I
Din I
Dpn I
Dpn II
Dra I
Ecl136 II
Eco105 I
Eco130 I
Eco147 I
Eco24 I
Eco32 I
Eco47 I
Eco88 I
Eco91 I
EcoICR I
EcoO65 I
EcoR I
EcoR II
EcoR I
EcoR V
EcoT14 I
EcoT38 I
Ege I
Ehe I
Erh I
FauND I
Fba I
Fbl I
Fok I
FriO I
Fun II
Hae III
Hap II
Hinc II
Hind II
Hind III
Hinf I
Hpa I
Hpa II
Kas I
Kpn I
Kpn2 I
Ksp I
Ksp22 I
KspA I
Kzo9 I
Mbo I
Mbo II
Mlu I
Mly113 I
Mnl I
Mro I
MroN I
Msp I
MspC I
Mva I
Mvr I
Nae I
Nar I
phone: +49-3641-6285 000
fax:
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e-mail:[email protected]
www.jenabioscience.com
Cleavage Site 5 ➞ 3
CTGCA[G
[GATC
CC[WGG
CC[WGG
G[GTNACC
TAC[GTA
CCANNNNN[NTGG
AT[CGAT
GG[CC
AT[CGAT
GGATG (2/0)
GC[GGCCGC
R[CCGGY
G[GNCC
CCGC[GG
C[CCGGG
AT[CGAT
CG[GWCCG
CG[GWCCG
TT[CGAA
G[TAC
A[CCGGT
GGC[GCC
GA[TC
[GATC
TTT[AAA
GAG[CTC
TAC[GTA
C[CWWGG
AGG[CCT
GRGCY[C
GAT[ATC
G[GWCC
C[YCGRG
G[GTNACC
GAG[CTC
G[GTNACC
G[AATTC
[CCWGG
G[AATTC
GAT[ATC
C[CWWGG
GRGCY[C
GGC[GCC
GGC[GCC
C[CWWGG
CA[TATG
T[GATCA
GT[MKAC
GGATG (9/13)
GRGCY[C
G[AATTC
GG[CC
C[CGG
GTY[RAC
GTY[RAC
A[AGCTT
G[ANTC
GTT[AAC
C[CGG
G[GCGCC
GGTAC[C
T[CCGGA
CCGC[GG
T[GATCA
GTT[AAC
[GATC
[GATC
GAAGA (8/7)
A[CGCGT
GG[CGCC
CCTC (7/6)
T[CCGGA
G[CCGGC
C[CGG
C[TTAAG
CC[WGG
CGAT[CG
GCC[GGC
GG[CGCC
Enzyme Finder
JBS Enzyme
Pst I
{Dpn I}, Mbo I, Sau3A I
BseB I
BseB I
BstE II
SnaB I
BstX I
BseC I
BshF I
BseC I
{Fok I}
Not I
BssA I
PspP I
SgrB I
{Sma I}
BseC I
Rsr II
Rsr II
Asu II
{Rsa I}
CspA I
{Nar I}
Dpn I, {Mbo I}, {Sau3A I}
{Dpn I}, Mbo I, Sau3A I
Dra I
{Sst I}
SnaB I
Sty I
SseB I
Ban II
EcoR V
Sin I
Ava I
BstE II
{Sst I}
BstE II
EcoR I
{BseB I}
EcoR I
EcoR V
Sty I
Ban II
{Nar I}
{Nar I}
Sty I
Nde I
Bcl I
Acc I
Fok I
Ban II
EcoR I
BshF I
BsiS I
Hinc II
Hinc II
Hind III
Hinf I
Hpa I
BsiS I
{Nar I}
Kpn I
BseA I
SgrB I
Bcl I
Hpa I
{Dpn I}, Mbo I, Sau3A I
{Dpn I}, Mbo I, Sau3A I
Mbo II
Mlu I
Nar I
Mnl I
BseA I
{Nae I}
BsiS I
MspC I
BseB I
Pvu I
Nae I
Nar I
Enzyme
Nco I
Nde I
Nde II
NgoM IV
Nhe I
Not I
Nru I
Nsp III
Nsp V
Pae I
PaeR7 I
Pal I
Pau I
Pce I
Pdi I
PflF I
Pho I
PinA I
Ple19 I
Psp124B I
Psp6 I
PspA I
PspE I
PspG I
PspOM I
PspP I
Pst I
Psy I
Pvu I
Pvu II
Rsa I
Rsr II
Rsr2 I
Sac I
Sac II
Sal I
Sau3A I
Sau96 I
Sca I
Sfi I
Sfo I
Sfr274 I
Sfr303 I
Sfu I
SgrB I
Sin I
Sla I
Sma I
SnaB I
SpaH I
Spe I
Sph I
SseB I
Ssp I
Sst I
Stu I
Sty I
Taq I
Tel I
Tli I
Tth111 I
Vha464 I
VpaK11B I
Xba I
Xho I
Xma I
XmaC I
Xmi I
Zho I
Zrm I
Cleavage Site 5 ➞ 3
C[CATGG
CA[TATG
[GATC
G[CCGGC
G[CTAGC
GC[GGCCGC
TCG[CGA
C[YCGRG
TT[CGAA
GCATG[C
C[TCGAG
GG[CC
G[CGCGC
AGG[CCT
GCC[GGC
GACN[NNGTC
GG[CC
A[CCGGT
CGAT[CG
GAGCT[C
[CCWGG
C[CCGGG
G[GTNACC
[CCWGG
G[GGCCC
G[GNCC
CTGCA[G
GACN[NNGTC
CGAT[CG
CAG[CTG
GT[AC
CG[GWCCG
CG[GWCCG
GAGCT[C
CCGC[GG
GTCGAC
[GATC
G[GNCC
AGT[ACT
GGCCNNNN[NGGCC
GGC[GCC
C[TCGAG
CCGC[GG
TT[CGAA
CCGC[GG
G[GWCC
C[TCGAG
CCC[GGG
TAC[GTA
GCATG[C
A[CTAGT
GCATG[C
AGG[CCT
AAT[ATT
GAGCT[C
AGG[CCT
C[CWWGG
T[CGA
GACN[NNGTC
C[TCGAG
GACN[NNGTC
C[TTAAG
G[GWCC
T[CTAGA
C[TCGAG
C[CCGGG
C[CCGGG
GT[MKAC
AT[CGAT
AGT[ACT
Enzyme available from
Jena Bioscience
Isoschizomer available
from Jena Bioscience
JBS Enzyme
Nco I
Nde I
{Dpn I}, Mbo I, Sau3A I
{Nae I}
Nhe I
Not I
Nru I
Ava I
Asu II
Sph I
Sla I
BshF I
BssH II
SseB I
Nae I
Tth111 I
BshF I
CspA I
Pvu I
Sst I
{BseB I}
{Sma I}
BstE II
{BseB I}
{Apa I}
PspP I
Pst I
Tth111 I
Pvu I
Pvu II
Rsa I
Rsr II
Rsr II
Sst I
SgrB I
Sal I
{Dpn I}, Mbo I, Sau3A I
PspP I
Sca I
Sfi I
{Nar I}
Sla I
SgrB I
Asu II
SgrB I
Sin I
Sla I
Sma I
SnaB I
Sph I
Spe I
Sph I
SseB I
Ssp I
Sst I
SseB I
Sty I
Taq I
Tth111 I
Sla I
Tth111 I
MspC I
Sin I
Xba I
Sla I
{Sma I}
{Sma I}
Acc I
BseC I
Sca I
Neoschizomer available
from Jena Bioscience
• Single Letter Code: R = A or G, Y = C or T, M = A or C, K = G or T, S = C or G, W = A or T, H = A or C or T, B = C or G or T, V = A or C or G, D = A or G or T, N = A or C or G or T
• Isoschizomers have same recognition sequence and cutting pattern.
• Neoschizomers (same recognition sequence but different cutting pattern) are indicated with brackets {enzyme}.