Thermo Scientific
Fermentas FastDigest
Restriction Enzymes
100% activity of all 176 FastDigest enzymes in the universal buffer
Complete digestion in 5 minutes
Direct loading of reaction mixture on gels
Thermo Scientific Fermentas FastDigest
Restriction Enzymes
Thermo Scientific Fermentas FastDigest enzymes are
an advanced line of restriction enzymes for rapid DNA
digestion. During our 30 years of restriction enzyme
research, we compiled one of the largest collections
of restriction enzyme producing bacterial strains in the
industry. Our large selection of enzyme isoschisomers
and state-of-the-art production facility facilitated the
creation of the unique system of 176 FastDigest
restriction enzymes.
Features
100% activity of all FastDigest enzymes
in the universal reaction buffer
Complete digestion in 5 - 15 minutes
100% buffer compatibility with
downstream applications
Direct loading on gels
176 FastDigest enzymes available
All FastDigest enzymes are 100% active in the universal FastDigest
and FastDigest Green buffers and are able to digest DNA in 5-15
minutes. This enables any combination of restriction enzymes to
work simultaneously in one reaction tube and eliminates the need
for sequential digestions. As an added convenience, the FastDigest
Green Buffer allows for direct loading of the reaction mixture on gels.
FastDigest enzymes are qualified for complete digestion of plasmid
DNA, genomic DNA, viral DNA and PCR products. Digestion time and protocols are experimentally proved and provided for each of the FastDigest enzyme on each of the templates.
Applications
Fast clone analysis
Fast preparation of DNA for cloning
Digestion of PCR products
Fast RFLP genotyping
Digestion of difficult-to-cleave DNA
Our FastDigest line of restriction enzymes is ideal for use in
applications that require high purity reaction components,
performance reliability and simple reaction set-up.
100 % Buffer Compatibility with all downstream applications
DNA/RNA modifying enzyme
Activity in FastDigest
Green Buffer/
FastDigest Buffer
100% activity of DNA modifying enzymes in
FastDigest and FastDigest Green Buffer
DNA Polymerase I, E.coli
100%
Klenow Fragment
100%
Klenow Fragment, exo
100%
T7 DNA Polymerase
100%
Enzymes for downstream applications can
be added directly to the restriction digestion
reaction mixture
75-100%
Eliminates the need for DNA purification steps
T4 DNA Ligase*
FastAP™ Thermosensitive
Alkaline Phosphatase
100%
T4 Polynucleotide Kinase
100%
* 0.5 mM ATP is required for T4 DNA Ligase activity.
www.thermoscientific.com/fastdigest
Universal buffer and direct loading on gels
All FastDigest restriction enzymes are 100% active in FastDigest
Green Buffer. In addition to the buffer components, a density reagent
and two tracking dyes are included for direct loading of digestion
reaction products on gels. The blue dye migrates with 3-5 kb DNA
fragments in a 1% agarose gel and has an excitation peak of 424 nm.
The yellow dye migrates faster than 10 bp DNA fragments in a 1%
agarose gel and has an excitation peak of 615 nm.
A
B
3-5 kb
The FastDigest Green Buffer offers the same high performance
in DNA digestion and downstream applications as the colorless
FastDigest Buffer. For applications that require product analysis by
fluorescence excitation (e.g. concentration measurements in UV
light), we recommend using the colorless 10X FastDigest Buffer.
10 bp
FastDigest Green Buffer
Reaction mixture containing FastDigest Green Buffer:
A: Loaded into gel lane, before electrophoresis
B :Separated in a gel following electrophoresis
Double and multiple digestion in universal
buffer for any combination of enzymes
Eliminate sequential digestions and
buffer changes
Direct loading of reaction mixture
on gels
Five minute triple digestion of plasmid DNA followed by
ligation in FastDigest and FastDigest Green buffers.
FastDigest
Buffer
Saves DNA loading reagents and
pipetting time
FastDigest Green
Buffer
Includes two tracking dyes and a
density reagent
M
C
1
2
C
3
4
M
M:Thermo Scientific Fermentas GeneRuler Express DNA Ladder
(#SM1551)
C: Undigested plasmid DNA control
1: Plasmid DNA triple digested with FastDigest EcoRI,
FastDigest KpnI and FastDigest SmaI in FastDigest buffer
2: The digestion and ligation reaction mixture in
FastDigest buffer
3: Plasmid triple digested with FastDigest EcoRI, FastDigest
KpnI and FastDigest SmaI in FastDigest Green buffer
4: The digestion and ligation reaction mixture in FastDigest
Green buffer
DNA Digestion in 5 minutes
Double and multiple digestion in
one buffer in 5 - 15 minutes
Digestion of plasmid DNA with FastDigest and conventional
restriction enzymes.
FastDigest enzymes
Ideal for high throughput applications
Digestion times are provided for all types
of DNA templates: plasmid DNA, PCR
product, genomic DNA
Conventional enzymes
5 Min
60 min
5 Min
No overnight digestions are required for
any template
60 min
120 min
5 Min
No star activity - eliminates the need for
prolonged incubation times
M
C
1
2
3
M
C
4
5
6
M
M: GeneRuler™ 1 kb Plus DNA Ladder
C: Undigested plasmid DNA
1: Plasmid DNA digested in 5 min with FastDigest XhoI
2: Plasmid DNA digested in 5 min with FastDigest ApaI
3: Plasmid DNA double digested in 5 min with FastDigest XhoI
and FastDigest ApaI
4: Plasmid DNA digested in 1 hour with ApaI
5: Plasmid DNA digested in 1 hour with XhoI
6: Plasmid DNA double digested in 1x Tango buffer with 4- fold
excess of ApaI for 1 hour and then in 2x Tango buffer with
XhoI for 1 more hour.
Save over 2 hours when performing double digestion
Conventional restriction enzymes
1
Reaction mixture for ApaI
FastDigest restriction enzymes
~ 2 minutes
2
Incubation
60 minutes
3
Reaction mixture for Xhol
> 2 minutes
4
Incubation
60 minutes
5
Addition of Loading Dye
TOTAL TIME 1 minutes
> 2 hours
1
ONE reaction mixture with:
FastDigest ApaI and
FastDigest XhoI
2
Incubation
5 minutes
TOTAL TIME 7 minutes
~2 minutes
Set a new standard in DNA digestion
FastDigest Enzymes - 100% Compatibility and Rapid DNA Digestion!
OLD STANDARD
NEW STANDARD
Conventional restriction enzymes
FastDigest restriction enzymes
Buffer system
Up to 20 buffers
One universal buffer
Buffer change between enzymatic steps
Frequently necessary
Not necessary
Double/multiple restriction digestion
Limited due to buffer incompatibility
Unlimited - all 176 enzymes are 100%
active in the universal buffer
Reaction time for plasmid DNA,
genomic DNA, PCR product
1 hour - overnight
5 - 15 minutes
Direct loading of reaction mixture on gels
No
Yes
Compatibility with downstream applications
Partial
100%
Star activity
Dependent on enzyme properties and
reaction conditions
No star activity due to short
incubation time
Activity definition
1 unit of enzyme hydrolyzes 1 μg of
lambda DNA in 60 minutes in an optimal
buffer for an enzyme
1 μl of FastDigest enzyme cleaves 1 μg
of substrate DNA in 5 or 15 minutes in
FastDigest buffer
Industry Leading Manufacturing Process
• Production in Class D clean room facilities qualified and certified as
per EU directives and ISPE guidelines
• Stringent quality control
• Consistency and lot-to-lot reproducibility assured by ISO9001 & ISO14001
• ISO13845 for production of IVD products
• cGMP production under request
More than 30 years of experience in development of restriction enzymes
frl
ere
ov
e
c
dis
ym
e
rm
Fe
5
7
19
s
nta
7
7
19
d
e
nd
fou
st
Fir
u
5
8
19
cti
i
str
e
er
u
niq
on
z
en
st
Fir
re
zym
n
ne
o
cti
i
str
W
NE
6
0
20
d:
ne
lo
ec
DA
AN
ST
st
Fa
st
ge
Di
en
e
zym
d
tro
n
si
I
47
o
Ec
RD
ed
uc
C
d:
Over 35 Years of Excellence
>3000 restriction enzyme producing strains
>30% of all known restriction enzymes discovered
>30% of all known restriction enzymes cloned
18 unique restriction enzymes
176 FastDigest Enzymes available
FastDigest Green Buffer for direct loading on gels
www.thermoscientific.com/fastdigest
Thermo Scientific Fermentas FastDigest
Restriction Enzymes
Cat. No.
Product name
FD0994
FD0904
FD1484
FD1794
FD0944
FD0344
FD0324
FD0834
FD1464
FD1954
FD1634
FD0014
FD0024
FD0034
FD1394
FD1414
FD0044
FD1894
FD0914
FD2094
FD0384
FD0314
FD1564
FD0054
FD0055
FD1004
FD1014
FD2074
FD0724
FD1764
FD0074
FD0084
FD0094
FD1444
FD2044
FastDigest AatII
FastDigest Acc65I
FastDigest AccI (XmiI)
FastDigest AciI (SsiI)
FastDigest AclI (Psp1406I)
FastDigest AcuI (Eco57I)
FastDigest AfeI (Eco47III)
FastDigest AflII (BspTI)
FastDigest AgeI (BshTI)
FastDigest AjuI
FastDigest AleI (OliI)
FastDigest AluI
FastDigest Alw21I
FastDigest Alw26I
FastDigest AlwNI (CaiI)
FastDigest ApaI
FastDigest ApaLI (Alw44I)
FastDigest AscI (SgsI)
FastDigest AseI (VspI)
FastDigest AsiSI (SfaAI)
FastDigest AvaI (Eco88I)
FastDigest AvaII (Eco47I)
FastDigest AvrII (XmaJI)
FastDigest BamHI
FastDigest BamHI
FastDigest BanI (BshNI)
FastDigest BbsI (BpiI)
FastDigest BbvI (Lsp1109I)
FastDigest BclI
FastDigest BfaI (FspBI)
FastDigest BglI
FastDigest BglII
FastDigest BlpI (Bpu1102I)
FastDigest Bme1580I (BseSI)
FastDigest BmtI (BspOI)
FD1314
FD0464
FD1184
FD1974
FD1714
FD0474
FastDigest BplI
FastDigest BpmI (GsuI)
FastDigest Bpu10I
FastDigest BsaAI (Ppu21I)
FastDigest BsaBI (BseJI)
FastDigest BsaHI (Hin1I)
FD1084
FastDigest BsaJI (BseDI)
FD0874
FD0884
FD1454
FD0924
FD0894
FD0854
FD1204
FD0454
FD1814
FD0124
FD0134
FastDigest BseGI
FastDigest BseNI
FastDigest BseXI
FastDigest Bsh1236I
FastDigest BsiEI (Bsh1285I)
FastDigest BsiWI (Pfl23II)
FastDigest BslI (BseLI)
FastDigest BsmBI (Esp3I)
FastDigest BsmFI (FaqI)
FastDigest Bsp119I
FastDigest Bsp120I
React
Cat. No.
Product name
50
100
50
20
20
20
20
150
20
20
20
100
100
100
50
300
200
100
200
100
200
200
20
800
2500
300
20
100
300
50
200
200
FD0654
FastDigest Bsp1286I (SduI)
FD0934
FastDigest Bsp1407I
100
FD1404
FD1284
FD1744
FD1274
FD1264
FD0184
FD2134
FD1024
FD0704
FD0374
FD0144
FD0214
FD1884
FD1704
FD0224
FD1234
FD1724
FD0334
FD0244
FD0234
FD0254
FD0294
FD0394
FD1304
FD0264
FD0274
FD0275
FD0304
FD0444
FD1644
FD2144
FD1664
FD1224
FD2184
FD0154
FD1904
FD1854
FD0494
FD0504
FD0505
FD0804
FD0484
FD1034
FD0514
FD1574
FD1734
FD0534
FD0524
FD2084
FD0814
FD0824
FD0754
FastDigest BspCNI (BseMII)
FastDigest BspHI (PagI)
FastDigest BspMI (BveI)
FastDigest BsrBI (MbiI)
FastDigest BsrDI (BseMI)
FastDigest BsrFI (Cfr10I)
FastDigest BssHII (PteI)
FastDigest BstXI
FastDigest BstZ17I (Bst1107I)
FastDigest Bsu36I (Eco81I)
FastDigest ClaI (Bsu15I)
FastDigest Csp6I
FastDigest DdeI (HpyF3I)
FastDigest DpnI
FastDigest DraI
FastDigest DraIII (AdeI)
FastDigest DrdI (AasI)
FastDigest EagI (Eco52I)
FastDigest Eam1105I
FastDigest EarI (Eam1104I)
FastDigest Ecl136II
FastDigest Eco31I
FastDigest Eco91I
FastDigest EcoNI (XagI)
FastDigest EcoO109I
FastDigest EcoRI
FastDigest EcoRI
FastDigest EcoRV (Eco32I)
FastDigest EheI
FastDigest Fnu4HI (SatI)
FastDigest FokI
FastDigest FspAI
FastDigest FspI (NsbI)
FastDigest HaeII (BfoI)
FastDigest HaeIII (BsuRI)
FastDigest HgaI (CseI)
FastDigest HhaI
FastDigest HincII
FastDigest HindIII
FastDigest HindIII
FastDigest HinfI
FastDigest HinP1I (Hin6I)
FastDigest HpaI (KspAI)
FastDigest HpaII
FastDigest Hpy8I
FastDigest HpyF10VI
FastDigest Kpn2I
FastDigest KpnI
FastDigest MauBI
FastDigest MboI
FastDigest MboII
FastDigest MfeI (MunI)
20
50
50
100
20
50
50
20
20
20
20
50
200
100
20
100
100
20
100
100
50
100
20
20
200
200
React
Cat. No.
Product name
50
FD1374
FD0564
FD1074
FastDigest MlyI (SchI)
FastDigest MluI
FastDigest MnlI
50
20
100
100
50
100
100
50
100
200
50
25
50
100
50
100
100
200
100
200
800
2500
400
50
20
100
20
50
200
400
20
200
100
800
2500
400
200
50
200
20
20
100
300
20
50
50
50
FD2024
FastDigest MreI
FD1214
FD2174
FD2004
FD0544
FD1344
FD0964
FD0554
FD1524
FD0064
FD0574
FD0575
FD0584
FD0585
FD0974
FD1834
FD1154
FD1514
FD0594
FD2154
FD0734
FD1474
FD2204
FD1534
FD0714
FD1754
FD0364
FD0764
FD1434
FD2064
FD1334
FD2224
FD0614
FD0615
FD1554
FD0624
FD0634
FD1124
FD0744
FD1134
FD0644
FD2164
FD1934
FD0784
FD0194
FD1194
FD0434
FD1424
FD2114
FD2124
FD1164
FastDigest MscI (MlsI)
FastDigest MseI (SaqAI)
FastDigest MslI (RseI)
FastDigest MspI
FastDigest MssI
FastDigest Mva1269I
FastDigest MvaI
FastDigest NaeI (PdiI)
FastDigest NciI (BcnI)
FastDigest NcoI
FastDigest NcoI
FastDigest NdeI
FastDigest NdeI
FastDigest NheI
FastDigest NlaIII (Hin1II)
FastDigest NlaIV (BspLI)
FastDigest NmuCI
FastDigest NotI
FastDigest NruI (RruI)
FastDigest NsiI (Mph1103I)
FastDigest NspI (XceI)
FastDigest PacI
FastDigest PdmI
FastDigest PflMI (Van91I)
FastDigest PfoI
FastDigest PmlI (Eco72I)
FastDigest PpuMI (Psp5II)
FastDigest PshAI (BoxI)
FastDigest PsiI (AanI)
FastDigest PsyI
FastDigest PspFI
FastDigest PstI
FastDigest PstI
FastDigest PsuI
FastDigest PvuI
FastDigest PvuII
FastDigest RsaI
FastDigest RsrII (CpoI)
FastDigest SacI
FastDigest SalI
FastDigest SanDI (KflI)
FastDigest SapI (LguI)
FastDigest Sau3AI (Bsp143I)
FastDigest Sau96I (Cfr13I)
FastDigest SbfI (SdaI)
FastDigest ScaI
FastDigest ScrFI (Bme1390I)
FastDigest SexAI (CsiI)
FastDigest SfaNI (BmsI)
FastDigest SfcI (BfmI)
© 2011 Thermo Fisher Scientific Inc. All rights reserved. Affibody is a trademark of Affibody. All other trademarks are the property of
Thermo Fisher Scientific Inc. and its subsidiaries.
US Literature AB-0003511A01U
Euro Literature PB_2011_17
Gene Analysis
Customer Support:
US: [email protected]
Europe: [email protected]
Technical Support:
US: [email protected]
Europe: [email protected]
React
Cat. No.
Product name
100
100
50
FD1824
FD0664
FD0404
FD1254
FD0604
FastDigest SfiI
FastDigest SmaI
FastDigest SnaBI (Eco105I)
FastDigest SpeI (BcuI)
FastDigest SphI (PaeI)
20
50
50
20
400
100
50
200
50
200
100
300
300
1000
100
100
20
20
50
100
100
50
25
100
100
20
200
50
100
20
100
20
800
2500
150
20
400
100
20
200
200
20
20
40
100
20
100
100
50
20
20
FD0774
FastDigest SspI
FD0414
FD0424
FD1244
FD1364
FD1144
FD0674
FD1294
FD1654
FD1784
FD0984
FD1354
FD2104
FD1384
FD0684
FD0685
FD0694
FD0695
FastDigest StyI (Eco130I)
FastDigest StuI (Eco147I)
FastDigest SwaI (SmiI)
FastDigest TaaI
FastDigest TaiI
FastDigest TaqI
FastDigest TatI
FastDigest TauI
FastDigest TfiI (PfeI)
FastDigest Tru1I
FastDigest Tsp509I (TasI)
FastDigest TspRI (TscAI)
FastDigest XapI
FastDigest XbaI
FastDigest XbaI
FastDigest XhoI
FastDigest XhoI
React
150
200
50
50
20
100
200
100
200
20
50
400
20
20
50
50
100
100
100
300
750
400
1200
For further information visit:
www.thermoscientific.com/fastdigest