Litpak 369270
J-TB-095AB
April 2014
Using the 500-mL Adapter
for the JLA-8.1000 and JLA-9.1000 Rotors
KIT CONTENTS
500-mL Adapter Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363683
contains
Adapter, PET (polyethylene teraphthalate), qty/1
Adapter cap, polycarbonate, qty/1
Adapter O-rings, silicone, qty/2 (replacement part number 970883)
DESCRIPTION
The patented* 500-mL adapter (see Figure 1) for the Beckman Coulter JLA-8.1000 and JLA-9.1000 rotors
enables the use of 500-mL, 250-mL, 50-mL, and 15-mL bottles and tubes, and Bio-Vials, in these rotors.
Labware smaller than 500 mL must be placed in another adapter, or more than one other adapter, before
being placed in the 500-mL adapter. See Table 1 for a list of bottles, tubes, and adapters that can be used.
Adapter Cap
Adapter O-rings
(970883)
Adapter Body
Figure 1. The 500-mL Adapter for the JLA-8.1000 and JLA-9.1000 Rotors
* U.S.
patent no. 5,901,873
© 2002—2014 Beckman Coulter, Inc.
INSTRUCTIONS
These instructions describe use of the 500-mL adapter assembly only. For complete information on use
and care of the JLA-8.1000 and JLA-9.1000 rotors, see the rotor manual (publication J-TB-073).
1. Prepare the 500-mL adapter by making sure that the two O-rings are in place as shown in Figure 1.
Do not lubricate the O-rings.
2. Fill and seal the bottles or tubes to be used in the 500-mL adapters.
3. If 500-mL bottles are used, place each bottle directly into a 500-mL adapter. For bottles of different
sizes, first load the bottles into the adapters listed in Table 1 and then place the filled bottle/adapter
assembly into the 500-mL adapters. Do not load sample directly into the 500-mL adapter.
4. Place the adapter cap on the adapter, push down, and twist. The bottom edge of the adapter cap
will not touch the top of the adapter, but the assembly is sealed by the O-rings and will seal
completely during centrifugation.
5. Hold the adapter by the adapter body, not by the adapter cap, and place the filled adapter into
a JLA-8.1000/JLA-9.1000 rotor cannister. No special orientation of the adapter in the cannister is
required. Make sure that six filled cannisters are loaded in the JLA-8.1000 rotor, or four cannisters
in the JLA-9.1000 rotor, prior to centrifugation.
6. After centrifugation, remove the 500-mL adapters from the cannisters by grasping each adapter cap
top and pulling up until you can grasp the adapter body. Then, holding the adapter body, remove the
adapter completely and set it on a flat surface.
7. To remove the adapter cap, hold the adapter firmly in one hand and, with the other hand, twist and
lift off the cap. Placing your fingers in the indentations in the adapter may help you to grip the
adapter tightly.
Some users may find the adapter caps difficult to remove. To facilitate removal, a rubber gripping
tool is available. This tool holds the adapter in place on a flat surface, leaving both of the user’s
hands free to remove the cap. The grip is contained in Tool Kit 366770, which may be ordered from
Beckman Coulter.
MAINTENANCE
Cleaning
Clean the adapters and adapter caps using a mild detergent, such as Beckman Solution 555. Rinse and dry
thoroughly. The O-rings may be left in place during cleaning unless leakage has occurred, in which case
you should remove the O-rings with a hemostat or similar instrument and thoroughly wash and dry them
before replacing them on the adapter. Do not lubricate the O-rings.
Decontamination
If components are contaminated with radioactive, toxic, or pathogenic materials, follow appropriate
decontamination procedures as outlined by your laboratory safety officer.
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Table 1. Available Tubes and Bottles for Use in the 500-mL Adapter
for the JLA-8.1000 and JLA-9.1000 Rotors.
Use only the items listed here and observe fill volumes and maximum run speeds.
Tube
Dimensions
and Volume
a
Description
Required Accessory
Part
Number
Max
Fill Vol a
(mL)
Description
Part
Number
Max Speed b/
RCFc
69 × 160 mm
500 mL
polycarbonate
bottle w/cap assy
355605
465
none
—
9 000 rpm
15 900 × g
69 × 160 mm
500 mL
polypropylene bottle
w/cap assy
355607
445
none
—
8 000 rpm
(both rotors)
15 200 × g
69 × 160 mm
500 mL
polycarbonate
bottle w/screw-cap
355664
465
none
—
9 000 rpm
15 900 × g
69 × 160 mm
500 mL
polypropylene bottle
w/screw-cap
355665
445
none
—
5 000 rpm
(both rotors)
5 900 × g
(JLA-8.1000)
4 900 × g
(JLA-9.1000)
62 × 120 mm
250 mL
polypropylene wide
mouth bottle w/cap
assy
356011
250
adapter
362750
(PET)
9 000 rpm
16 800 × g
62 × 120 mm
250 mL
polycarbonate wide
mouth bottle w/cap
assy
356013
250
adapter
362750
(PET)
9 000 rpm
16 800 × g
29 × 104 mm
50 mL
polycarbonate
bottle assy w/liquidtight cap assy
357000
45
adapter
356996
(polypropylene)
9 000 rpm
—
29 × 104 mm
50 mL
polycarbonate
bottle w/screw cap
357002
40
adapter
356996
(polypropylene)
9 000 rpm
—
29 × 104 mm
50 mL
polypropylene bottle
assy w/liquid-tight
cap assy
357001
45
adapter
356996
(polypropylene)
9 000 rpm
—
29 × 104 mm
50 mL
polypropylene bottle
w/screw cap
357003
40
adapter
356996
(polypropylene)
9 000 rpm
—
29 × 104 mm
50 mL
polypropylene
thickwall tube
w/snap-on cap
357005
36.5
adapter
356996
(polypropylene)
9 000 rpm
—
natural 29-mm cap
red 29-mm cap
green 29-mm cap
yellow 29-mm cap
blue 29-mm cap
356264 d
357359 d
357360 d
357361 d
357362 d
Above 20°C fill polypropylene tubes at least half full.
Continued —
b Maximum
speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter,
but, because of manufacturing variances, no guarantee of performance or fit is expressed or implied.
c
Maximum speeds and g-forces listed are for the JLA-9.1000 rotor except as noted. The maximum speed of all components
in the JLA-8.1000 rotor is 8000 rpm except as noted.
d Polypropylene;
package of 25.
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Table 1. Available Tubes and Bottles for Use in the 500-mL Adapter
for the JLA-8.1000 and JLA-9.1000 Rotors (continued)
Tube
Dimensions
and Volume
a
Description
Part
Number
Max
Fill
Vol a
(mL)
Required Accessory
Description
Part
Number
Max Speed b/
RCF
29 × 104 mm
50 mL
polypropylene
thickwall tube,
no cap
357007
50
adapter
356996
(polypropylene)
9 000 rpm
—
29 × 104 mm
50 mL
polycarbonate
open-top tube
363647
40
adapter
356996
(polypropylene)
9 000 rpm
—
29 × 104 mm
50 mL
polycarbonate
tube w/snap-on
cap
363664
40
adapter
356996
(polypropylene)
9 000 rpm
—
17 × 119 mm
15 mL
conical
polypropylene
open-top tube
355663
15
adapter
356960
(polypropylene)
6 500 rpm
(both rotors)
—
Bio-Vials
14 × 55 mm
4 mL
polypropylene,
capped
566353
4
adapter
342098
(delrin)
and
362750
(PET)
9 000 rpm
—
Above 20°C fill polypropylene tubes at least half full.
b Maximum
speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but, because of
manufacturing variances, no guarantee of performance or fit is expressed or implied.
c
Maximum speeds and g-forces listed are for the JLA-9.1000 rotor except as noted. The maximum speed of all components in the
JLA-8.1000 rotor is 8000 rpm except as noted.
d Polypropylene;
package of 25.
Sterilization and Disinfection
• Ethanol (70%)† may be used on all components. See Appendix A in Rotors and Tubes for Beckman
Coulter Avanti J, J2, and J6 Series Centrifuges (publication JR-IM), or publication IN-175, for
more information regarding chemical resistances of rotors and accessories.
• The adapter, adapter cap, and O-rings can be autoclaved at 121°C for 15 minutes. Repeated autoclaving will reduce the life of these components. For this reason, cold sterilization methods are
recommended.
While Beckman Coulter has tested these methods, no guarantee of sterility or disinfection is expressed
or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer.
† Flammability
4
hazard. Do not use in or near operating centrifuges.
Trademarks
Beckman Coulter, the stylized logo, and Avanti are trademarks of Beckman Coulter, Inc. and are
registered in the USPTO.
All other trademarks, service marks, products, or services are trademarks of their respective
holders.
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Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 U.S.A.
Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com • Printed in U.S.A.
© 2014 Beckman Coulter, Inc.
All rights reserved