® easySpiral The world's fastest Spiral® plater Our quality for your lab • Designer and manufacturer for microbiology • R&D leadership for innovative and reliable products • Worldwide distribution network in more than 85 countries • Made in France interscience R&D center and manufacturing plant Mourjou FRANCE ® Spiral method: 35 years experience The Spiral® method was designed in 1973 to automate the routine work of bacterial enumeration by Dr. Ed Campbell, researcher at the FDA (Food & Drug Administration). With François Jalenques, friend and founder of interscience, they patented an updated method in 1992. Since then Spiral® automatic platers have been a reference for applications in food microbiology, medical bacteriology, research on food preservatives or cosmetological factors in compliance with the NF V08-100, NF V08-050, NF V08-034, ISO 7218, ISO 4833-2, ISO 15189, FDA-BAM and AOAC standards. interscience has proudly launched the 3rd generation of Spiral® platers with easySpiral® and easySpiral Pro®. François Jalenques & Dr. Ed Campbell Spiral® DS First Spiral Plater® 1979 Spiral® DS+ Automatic Spiral Plater® 1996 easySpiral®/easySpiral Pro® World fastest Spiral® platers Worldwide patented 2010 2 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com ® What is Spiral method? With easySpiral® automatic plater, increase your lab capacity with a counting range from 100 to 1x107 CFU/mL on one single Petri dish without prior dilution. Automatic Spiral® method Manual plating method This method requires repetitive actions: at least 4 dilutions and 4 successive platings are necessary to obtain one good and readable Petri dish. With this method, make your analyses on 1 single Petri dish! + sample - sample dilution 1/10 dilution 1/10 dilution 1/10 dilution 1/10 n tions o u l i d l l A dish! 1 Petri ® l Pro easySpira spread 0.1 mL spread 0.1 mL spread 0.1 mL spread 0.1 mL 1st Petri dish 2nd Petri dish 3rd Petri dish 4th Petri dish Incubation Incubation Incubation Disinfectant Sterile water Incubation Incubation • From 100 to 1x107 CFU/mL on 1 single Petri dish • Up to 75% less consumables • Full plating cycle in 25 seconds! How to count? With the Spiral® method, a logarithmically decreasing volume of sample is dispensed on the surface of a rotating Petri dish in an Archimedes spiral. The volume is calibrated and known at every point of the Petri dish. Bacterial concentration is determined by dividing the number of colonies found by the volume dispensed in the same sector of the dish. Manual colony counting Automatic colony counting Scan® 100 or ® with Spiral counting grid For manual counting, count the colonies in the first A or B outer segment and reach a minimum of 20 colonies. Repeat the same operation on the opposite side of the Petri dish. Scan® 300 Scan® 500 Scan® 1200 Scan® 4000 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com 3 easySpiral revolutionary efficiency ® The new easySpiral® patented technology, exclusively developed by interscience, allows the automatic plating of your sample on a Petri dish in only 25 seconds with a decreasing concentration (counting range: from 100 to 1x107 CFU/mL). A completely new architecture with a revolutionary rotating arm allows a full cycle in 25 seconds (disinfection, sample-taking and plating). All the liquid flows are visible. No exterior maintenance is needed. easySpiral® is equipped with an innovative unique stylus cleaning device, which guarantees a perfect cleaning and disinfection of the stylus, with no risk of cross-contamination. Autoclavable bottles Secure and easy connection by CPC Connectors 2 L bottles 600 cleaning cycles Auto disinfection The inside and outside of the stylus are cleaned by overflow technology No cross contamination PATENTED Sampling area Fast and accurate filling in a sterile beaker DB50, sterile and autoclavable Stainless steel housing Compact, 40 cm wide Can be used inside and outside a laminar flow 4 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com easySpiral® ensures fast sample platings with 99.5% repeatability and reproducibility, and reliable analyses by harmonizing the work of the whole lab team. Cleaning liquid sensor Alarm when the bottle is empty Rotating arm architecture Automatic full cycle: 25 seconds PATENTED Modular Petri dish stand Switch from 90 to 150 mm Petri dishes Intuitive control panel Easy to use No training needed Monthly cleaning 1 button to access the intensive, periodic cleaning cycle (10 mn approx.) NF NF XP ISO ISO ISO V08-100 V08-050 V08-034 7218 4833-2 15189 FDA BAM Bacteriological Analytical Manual AOAC 977.27 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com 5 easySpiral® technology ,QWHUQDWLRQDO 3$7(176 Delivering the fastest cycle time ever, easySpiral® has an impressive rotating arm architecture, new patented disinfection system avoiding risks of cross-contamination and full traceability of the operations with a counting range from 100 to 1x107 CFU/mL on 1 Petri dish. The fastest Spiral® plater: only 25 seconds for a full cycle! • 25 seconds for a full cycle (disinfection, sample-taking and plating) • Rotating arm with high speed movement • Patented electronic robot system • 10 Petri dishes plated in less than 2 mn (with the same sample) Overflow technology* sample drains away cleaning with alcohol rinsing with sterile water • No cross-contamination • Unique patented disinfection system by overflow • 600 cleanings without changing bottles (2L) USB programmable volume & traceability** • Easy PC connectivity via USB • From 10 to 1000 microliters programmable volumes • 3 preset plating modes • Monitoring software with traceability XLS Excel™ USB Consumables reduced up to 75%! • Reduced budget for consumables • No specific consumables needed • More space in your laboratory *Scientific study available upon request **On easySpiral Pro® 6 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com ® Plate & Count System + dataLink™ Plate & Count System® + dataLinkTM offer quick and reliable results from automatic plating to colony counting with total traceability. GREAT SAVINGS: Up to 75 % savings in time, consumables and space QUICK: Full plating cycle in 25 seconds and counting in 1 click. No need of manual data input as the Scan® colony counter retrieves it and adjusts automatically. RELIABLE: Repeatable and reproducible results up to 98 % FULL TRACEABILITY WITH Automatic saving of data and results dataLinkTM: easySpiral Pro® and Scan® 4000 How does it work ? STEP 1 Plate with easySpiral Pro®. easySpiral® software collects the plating data. ® al Pro easySpir STEP 2 Print the label with Datamatrix code. Stick the label on the plated Petri dish and place in the incubator. … 24-72 h incubation AUTO PDF JPG JPEG Scan 4000 Adobe XLS Excel™ LIMS STEP 3 Once the colonies have grown, scan the Datamatrix code. The Scan® colony counter automatically adjusts its settings thanks to the Datamatrix label’s data. Click on “COUNT”. Export the data. * Please check LIMS compatibility D DOWNLOAD S C ® SOFTWARE SCAN www.interscience.com 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com 7 easySpiral Pro® 412 000 413 000 Syringe capacity APPLICATIONS 1000 µL Preset deposited volume Counting range 50 or 100 µL 50, 100 or 200 µL 300 to 1.3 x 105 CFU/mL 100 to 1 x 107 CFU/mL 25 seconds Plating time (50 µL - disinfection + plating) Overflow technology • Microbiological analyses • Food quality control • Bacterial cinetics 8 bars pressure Disinfectant autonomy (in normal mode) 1000 cycles (2L bottles) Distilled water autonomy (in normal mode) 600 cycles (2L bottles) • MIC of antibiotics Successive plating capacity with the same sample Up to 20 Petri dishes (50 µL) • Challenge test: cosmetics control Automatic disinfection program ✔ ✔ • Pharmaceutical control 90 mm Petri dish ✔ ✔ - ✔ Exponential deposition mode ✔ ✔ Circle deposition mode ✔ ✔ Constant deposition mode - ✔ Excel™ export traceability - ✔ Programmable deposited volume (via USB) - from 10 to 1000 µL 150 mm Petri dish Dimensions 40 x 41.5 x 29 cm Weight 16.4 Kg Power supply 100-240 V~ 50-60 Hz Delivered with: 1 blue dye for testing, 1 syringe with silicone tubing, Spiral® counting grids: 90 mm & 150 mm*, support test container, 2 filters, 1 replacement stylus, 4 connector sets for GL45 bottles, 1 GL45 double connection connector set, 1 pot of EnzyClear®, 1000 sterile beakers, power cord, user manual, 150 mm Petri dish ring*, software on a CD-ROM*, USB cable*. *Only for easySpiral Pro® Accessories Housing protection for platers dataLink™ DB 50 beakers Bar-code reader Transparent housing for working outside laminar flow Ref 413 001 Traceability system Ref 410 100 1 000 sterile beakers for Spiral® platers Ref 415 100 Barcode reader for traceability Ref 522 000 INTERNATIONAL Your local distributor 3 0, c h e m i n du Bois d e s A r p e nts - 78 8 6 0 S t N o m - FR A NCE T: +33 (0)1 34 62 62 61 - F: +33 (0)1 34 62 43 03 - Email: [email protected] USA & CANADA 32 Cu m m in g s Pa r k - Wo bu r n, M A 018 01 - USA T: +1 781 937 0007 - F: +1 781 937 017 - Email: [email protected] CHINA 上海市徐汇区吴 兴路277号锦都大 厦5 8 8室 - 20 0 0 3 0 电话: +86 (0)21-64739390 - +86 189 3097 0733 - 邮址: [email protected] SOUTH-EAST ASIA www. interscience.com 5 41 O r c h a r d r o a d - 0 9 - 0 1 L i a t To w e r s - S I N G A P O R E 2 3 8 8 8 1 T: +65 6933 1389 - +65 8549 1217 - Email: [email protected] 3073 easySpiral_B_0216. Pictures and information in this brochure are not contractually binding. Spiral®, easySpiral®, easySpiral Pro®, Spiral Plater®, Plate & Count System®, Scan® are registered trademarks. INTERSCIENCE reserves the right to change or improve the specifications of its products without notice. Please visit www.interscience.com for current updates and additional information. RCS 950 356 220 Versailles. INTERSCIENCE SARL F78860. Reference easySpiral® EN Technical specifications ® easySpiral Dilute The world's first automatic diluter & plater A revolution in serial dilutions! Our quality for your lab • Designer and manufacturer for microbiology • R&D leadership for innovative and reliable products • Worldwide distribution network in more than 85 countries interscience R&D center and manufacturing plant Mourjou FRANCE • Made in France ® Spiral method: 35 years experience The Spiral® method was designed in 1973 to automate the routine work of bacterial enumeration by Dr. Ed Campbell, researcher at the FDA (Food & Drug Administration). With François Jalenques, friend and founder of interscience, they patented an updated method in 1992. Since then Spiral® automatic platers have been a reference for applications in food microbiology, medical bacteriology, research on food preservatives or cosmetological factors in compliance with the NF V08-050, NF V08-034, ISO 7218, ISO 4833-2, ISO 15189, FDA BAM and AOAC 977.27 standards. Today interscience is proud to launch the 4th generation of Spiral® platers with easySpiral Dilute®: the world's first automatic diluter & plater. F. Jalenques & Dr. Ed Campbell 1979 Spiral® DS First Spiral® plater 1996 Spiral® DS+ Automatic Spiral® plater 2010 easySpiral® World's fastest Spiral® plater PATENTED 2012 easySpiral Dilute® World's first automatic diluter & plater PATENTED interscience is proud to introduce easySpiral Dilute®, a 2-in-1 automatic diluter and plater, which allows you to do 5 x 1/10th serial dilutions. It will then automatically plate on 1 single Petri dish, with a countable range from 30 to 1 x 1012 countable CFU/mL. No more manual dilutions and plating is automatic! 2 Plate Place sample 1 Dilute Place your sample in the beaker 1. The easySpiral Dilute® will automatically dilute the sample up to 5 times 2. The easySpiral Dilute® will automatically plate the sample on a Petri dish up to 1 x 1012 countable CFU/mL Why use an automatic diluter & plater? easySpiral Dilute® allows you to save 50% on consumables and 50% on your time. Comparison between manual and automatic method below. Example of 50 samples: Dilution: 10-7 Plating: 10-5, 10-6 and 10-7 Manual method Automatic method CONSUMABLES CONSUMABLES Dilution Dilution • 400 tubes • 350 beakers • 350 pipet tips Plating Plating • 450 Petri dishes • 150 Petri dishes TIME 10h10 TIME 5h50 3 Key features Compact High precision glass syringe 38 cm wide, can be used inside and outside a laminar flow Stainless steel housing Typical accuracy of 0.5 % 4x more precise than a basic pipet No more pipet calibration High speed automation Fast rotating arm 1 disinfection, 5 dilutions and 1 plating in 134 s PATENTED Automatic disinfection One-touch plating Overflow technology cleaning system No cross contamination Choose from 4 plating modes Plate on 55, 90 and 150 mm Petri dishes PATENTED Easy serial dilutions High capacity Choose your dilution factor Do 10-5 dilution or more automatically No more 9 mL tube preparation 2 fully autoclavable bottles (disinfectant & diluent) and connectors PATENTED 4 How does it work? 1. Dilution cycle 1 Sample intake sample 2 0.5 mL of sample is deposited sample 10-1 sample 10-1 3 4.5 mL of diluent is added to the sample and mixed The above dilution cycle is repeated for the next serial dilution. 0.5 mL of 10-1 diluted sample intake 1 sample 10-1 sample 10-1 2 0.5 mL of 10-1 diluted sample is deposited 10-2 Dilution cycle can be repeated as many times as you wish. 2. Plating cycle Constant mode Exponential mode Circle mode Pour mode Homogenous surface deposition Decreasing surface concentration 3 or 6 surface dilutions on 1 Petri dish Homogenous deposition* in pour plate mode 50 µL, 100 µL, 200 µL and USB programmable 50 µL, 100 µL, 200 µL and USB programmable 10 µL inner circle, 16 µL middle circle and 25 µL outer circle 50 µL, 100 µL, 200 µL and USB programmable Examples: Before incubation Homogenous surface deposition + concentrated - concentrated 10-1 10-2 10-3 Homogenous deposition in pour plate mode* After incubation Easy colony counting: No colonies on the edge of the Petri dish Counting on Petri dish up to 1 x 1012 CFU/mL Savings on Petri dishes Easy colony counting for invasive colonies. Pharmacopoeia compliant * In pour mode, easySpiral Dilute® deposits one sample drop at the bottom of an empty Petri dish. Then, the user adds the molten agar on top and homogenizes it with the sample. How to count? Manual colony counting Automatic colony counting Scan® 100 or with Spiral® counting grid Scan® 300 Scan® 500 Scan® 1200 Scan® 4000 5 Questions What is special about the easySpiral Dilute®? easySpiral Dilute® is the only 2-in-1 equipment on the market making automatic serial dilutions and standardized plating. The accuracy of easySpiral Dilute® is high: 0.5%. How can I check it? It is easy and automatic. Test of the deposited volume during plating: Press on the plating volume test button. This function will fill a previously weighed beaker with 500 µL. Weigh the distributed volume to check the results. Test of the volume of the dilutions: Press on the dilution volumes test button. This function will fill 2 previously weighed beakers: one with 0.5 mL and the 2nd one with 4.5 mL. Weigh the distributed volumes to check the results. Which diluents can I use? easySpiral Dilute® works with saline solutions (e.g.: Ringer's solution, physiological saline). Avoid using diluents with nutrients as they may cause contaminations. Bacteria only remain a few seconds in the beakers before being deposited on the Petri dish so using a saline diluent has no effect on the final enumeration. It is however possible to use nutrient solutions as diluent in the semi-automatic "My Diluent" mode. In that case, fill in the beakers with 4.5 mL diluent before processing. 6 I usually dilute 1 mL to 1/10 and the easySpiral Dilute® uses 0.5 mL to 1/10. Is easySpiral Dilute® as precise with a volume divided by 2? Usually the average accuracy for the 9 mL tubes filling and 1 mL pipetting is 2% with the need to calibrate and regularly check the pipets you are using. easySpiral Dilute® has an average accuracy of 0.5% on 0.5 mL and 4.5 mL volumes because it works with a high precision HamiltonTM glass syringe in a liquid displacement circuit. With volumes divided by 2, precision and repeatability are then much higher! I need to make a 10-7 dilution. How can I do it? The easySpiral Dilute® can do as many dilutions as you wish. Do a 10-5 dilution, take the last beaker and place it as the mother sample and then proceed with a new automatic dilution sequence. Accessories & technical specifications Housing protection dataLinkTM Beakers DB 50 Barcode reader Transparent housing for working outside laminar flow. Traceability system 1 000 sterile beakers for Spiral® platers (5 mL) Barcode reader for traceability Ref. 413 001 Ref. 410 100 Ref. 415 100 Ref. 522 000 easySpiral Dilute® is delivered with 1 000 sterile beakers, 1 blue dye for tests, 1 box of EnzyClear®, 4 sets of GL 45 bottle connectors, Petri dish rings: 55, 90 & 150 mm, Spiral® counting grids: 90 & 150 mm, circle counting grids: 90 & 150 mm, power cord, user’s manual, monitoring CD-ROM software, USB cable. Reference Petri dish diameter Syringe capacity Programmable dispensed volume (via USB) Preset dispensed volume Detection in CFU/mL of Spiral® plating Plating modes Number of dilutions at 1/10th for 1 cycle 1 disinfection + 5 dilutions + 1 plating of 1 dilution 1 disinfection + 5 dilutions + 1 plating of each dilution Disinfectant autonomy Diluent autonomy Circle mode (patented) Stylus disinfection system (patented) Pressure in the stylus Successive plating capacity with the same sample Traceability Monthly cleaning program Filling time programmable via USB My Diluent key Sample mixing before dilution/plating Controlled by microprocessor Dimensions (w x d x h) Weight Power supply Power 1 year guarantee (after registration) All stainless steel Made in France NF NF ISO ISO ISO V08-050 V08-034 7218 4833-2 15189 easySpiral Dilute® 414 000 55 mm, 90 mm and 150 mm 1 000 µL from 10 µL to 1 000 µL 50 µL, 100 µL and 200 µL 30 to 1 x 1012 CFU/mL 55 mm: constant, pour plate 90 mm: exponential (10-5), constant, circle (3 dilutions), pour plate 150 mm: exponential (10-7), constant, circle (6 dilutions), pour plate 5 134 s 234 s 1000 (with 2 L bottles) 63 cycles (with 2 L bottles) 3 dilutions on a 90 mm Petri dish 6 dilutions on a 150 mm Petri dish inside and outside by Overflow technology 8 bars up to 20 Petri dishes (50 µL) excelTM, datamatrix labels D D D D D 39 x 42 x 29 cm 15.6 kg 100-240 V~ / 50 Hz to 60 Hz 65 W D D D FDA BAM Bacteriological Analytical Manual AOAC 977.27 ISO 9001 2008 WEEE RoHS 2002/96/EC 2002/95/EC MADE IN FRANCE 7 USA & CANADA 199 Weymouth ST. - ROCKLAND - MA 02370 - USA T: +1 781 792 2133 - F: +1 781 792 2134 [email protected] - www.intersciencelab.com CHINA 上海市徐汇区吴兴路277号锦都大厦718室-200030 电话: +86 (0)21-64739390 - 手机: +86 189 3097 0733 [email protected] - www.interscience.fr/china Distributor www. interscience .fr easySpiral Dilute_B_0615. Pictures and information in this brochure are not contractually binding. Spiral®, easySpiral®, easySpiral Dilute®, Scan® are trademarks. This document is the translation of the french brochure and is not contractually binding. INTERSCIENCE reserves the right to change or improve the specifications of its products without notice. Please visit www.interscience.fr for current updates and additional information. RCS 950 356 220 Versailles. INTERSCIENCE SARL F78860. 30, ch. Bois Arpents - 78860 St Nom - FRANCE T : +33 (0)1 34 62 62 61 - F : +33 (0)1 34 62 43 03 i n f o @ i n t e r s c i e n c e .f r - w w w. i n t e r s c i e n c e .f r EN INTERNATIONAL Appendix 2. easySpiral® / easySpiral® Pro validation studies 1. Spiral plating method validation Purpose To study the reliability of Spiral plating with easySpiral® comparing to the reference method (classical surface plating). Equipment and methods Five bacterial strains (three gram+ and two gram-) are tested: Lactobacillus casei var. rhamnosus, Bacillus cereus (ATCC 11778), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 8739) and Pseudomonas aeruginosa (ATTC 9027). Bacterial cultures are diluted in buffered peptone water then plated on Petri dish agar (90 mm), using classical method and Spiral method (50!L in exponential mode). After 48h of incubation at 37 C° (±1) on MRS (De Man, Rogosa, Sharpe) for Lactobacillus casei and 18h (±2) at 37C° (±1) on PCA (Plate Count Agar) for Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa and on Macconkey for Escherichia coli, counting is made with an automatic colony counter (Scan®1200). Results analysis Counting results are expressed in Log CFU/mL (Colony Forming Unit) and a regression analysis is made to assess the correlation between, Spiral method with easySpiral® Pro and classical plating method. Results - Bacillus cereus: Spiral method (Log CFU/mL) 5 y = 0,974x + 0,0217 R2 = 0,982 4.5 4 3.5 3 2.5 2.5 3 3.5 4 4.5 5 Classical method (Log CFU/mL) Figure 1: Correlation between Bacillus cereus counting (Log CFU/mL) obtained by plating in classical and Spiral method. 85/125 ® ® easySpiral / easySpiral Pro : Guide d’installation et d’utilisation V3 - Bacillus subtilis: 5 Spiral method (Log CFU/mL) y = 0.9923x + 0.0238 2 R = 0.9853 4.5 4 3.5 3 2.5 2.5 3 3.5 4 4.5 5 Classical method (Log CFU/mL) Figure 2: Correlation between Bacillus subtilis counting (en Log CFU/mL) obtained by plating in classical and Spiral method. - Lactobacillus casei var. rhamnosus: Spiral method (Log CFU/mL) 5.5 y = 0,9036x + 0,335 2 R = 0,994 5 4.5 4 3.5 3 3 3.5 4 4.5 5 5.5 Classical method (Log CFU/mL) Figure 3: Correlation between Lactobacillus casei var. rhamnosus counting (en Log CFU/mL) obtained by plating in classical and Spiral method. ® ® easySpiral / easySpiral Pro: Installation guide and user’s manual V3 86/125 - Escherichia coli: Spiral method (Log CFU/mL) 5.5 y = 0,9822x + 0,0853 R2 = 0,989 5 4.5 4 3.5 3 3 3.5 4 4.5 5 5.5 Classical method (Log CFU/mL) Figure 4: Correlation between Escherichia coli counting (en Log CFU/mL) obtained by plating in classical and Spiral method. - Pseudomonas aeruginosa: Spiral method (Log CFU/mL) 5 y = 0,9632x + 0,1039 R2 = 0,990 4.5 4 3.5 3 2.5 2 2 2.5 3 3.5 4 4.5 5 Classical method (Log CFU/mL) Figure 5: Correlation between Pseudomonas aeruginosa counting (en Log CFU/mL) obtained by ® plating in classic and easySpiral pro method. Conclusion Results show a strong correlation (R2 " 0.982) between Spiral method with easySpiral® Pro and Classical method. Hence the average difference of CFU/mL log between the two methods is resulting to be not significatif 12 times lower than the maximal deviation (0.5 log) authorised by AFNOR NF V08/100. 87/125 ® ® easySpiral / easySpiral Pro : Guide d’installation et d’utilisation V3
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