easySpiral®

®
easySpiral
The world's fastest Spiral® plater
Our quality for your lab
• Designer and manufacturer for microbiology
• R&D leadership for innovative and reliable products
• Worldwide distribution network in more than 85 countries
• Made in France
interscience
R&D center and manufacturing plant
Mourjou FRANCE
®
Spiral method:
35 years experience
The Spiral® method was designed in 1973 to automate the routine
work of bacterial enumeration by Dr. Ed Campbell, researcher at
the FDA (Food & Drug Administration). With François Jalenques,
friend and founder of interscience, they patented an updated
method in 1992.
Since then Spiral® automatic platers have been a reference for
applications in food microbiology, medical bacteriology, research on
food preservatives or cosmetological factors in compliance with the
NF V08-100, NF V08-050, NF V08-034, ISO 7218, ISO 4833-2,
ISO 15189, FDA-BAM and AOAC standards.
interscience has proudly launched the 3rd generation of Spiral®
platers with easySpiral® and easySpiral Pro®.
François Jalenques & Dr. Ed Campbell
Spiral® DS
First Spiral Plater®
1979
Spiral® DS+
Automatic Spiral Plater®
1996
easySpiral®/easySpiral Pro®
World fastest Spiral® platers
Worldwide patented
2010
2
30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com
®
What is Spiral method?
With easySpiral® automatic plater, increase your lab capacity with a counting range from 100 to 1x107 CFU/mL
on one single Petri dish without prior dilution.
Automatic Spiral® method
Manual plating method
This method requires repetitive actions: at least 4
dilutions and 4 successive platings are necessary
to obtain one good and readable Petri dish.
With this method, make your analyses on 1 single
Petri dish!
+ sample
- sample
dilution
1/10
dilution
1/10
dilution
1/10
dilution
1/10
n
tions o
u
l
i
d
l
l
A
dish!
1 Petri
®
l Pro
easySpira
spread
0.1 mL
spread
0.1 mL
spread
0.1 mL
spread
0.1 mL
1st Petri
dish
2nd Petri
dish
3rd Petri
dish
4th Petri
dish
Incubation
Incubation
Incubation
Disinfectant
Sterile water
Incubation
Incubation
• From 100 to 1x107 CFU/mL
on 1 single Petri dish
• Up to 75% less consumables
• Full plating cycle in 25 seconds!
How to count?
With the Spiral® method, a logarithmically
decreasing volume of sample is dispensed
on the surface of a rotating Petri dish in
an Archimedes spiral. The volume is
calibrated and known at every point of
the Petri dish. Bacterial concentration
is determined by dividing the number of
colonies found by the volume dispensed
in the same sector of the dish.
Manual colony counting
Automatic colony counting
Scan® 100
or
®
with Spiral
counting grid
For manual counting, count
the colonies in the first A
or B outer segment and
reach a minimum of 20
colonies. Repeat the same
operation on the opposite
side of the Petri dish.
Scan® 300
Scan® 500
Scan® 1200
Scan® 4000
30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com
3
easySpiral revolutionary efficiency
®
The new easySpiral® patented technology, exclusively developed by interscience, allows the
automatic plating of your sample on a Petri dish in only 25 seconds with a decreasing concentration
(counting range: from 100 to 1x107 CFU/mL).
A completely new architecture with a revolutionary rotating arm allows a full cycle in 25 seconds
(disinfection, sample-taking and plating). All the liquid flows are visible. No exterior maintenance is needed.
easySpiral® is equipped with an innovative unique stylus cleaning device, which guarantees a perfect cleaning
and disinfection of the stylus, with no risk of cross-contamination.
Autoclavable bottles
Secure and easy connection
by CPC Connectors
2 L bottles
600 cleaning cycles
Auto disinfection
The inside and outside of the stylus
are cleaned by overflow technology
No cross contamination
PATENTED
Sampling area
Fast and accurate filling in a sterile beaker
DB50, sterile and autoclavable
Stainless steel housing
Compact, 40 cm wide
Can be used inside and outside a laminar flow
4
30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com
easySpiral® ensures fast sample platings with 99.5%
repeatability and reproducibility, and reliable analyses
by harmonizing the work of the whole lab team.
Cleaning liquid sensor
Alarm when the bottle is empty
Rotating arm architecture
Automatic full cycle: 25 seconds
PATENTED
Modular Petri dish stand
Switch from 90 to 150 mm Petri dishes
Intuitive control panel
Easy to use
No training needed
Monthly cleaning
1 button to access the intensive,
periodic cleaning cycle (10 mn approx.)
NF
NF
XP
ISO
ISO
ISO
V08-100
V08-050
V08-034
7218
4833-2
15189
FDA BAM
Bacteriological Analytical
Manual
AOAC
977.27
30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com
5
easySpiral® technology
,QWHUQDWLRQDO
3$7(176
Delivering the fastest cycle time ever, easySpiral® has an impressive rotating arm architecture, new patented
disinfection system avoiding risks of cross-contamination and full traceability of the operations with a counting
range from 100 to 1x107 CFU/mL on 1 Petri dish.
The fastest Spiral® plater:
only 25 seconds for a full cycle!
• 25 seconds for a full cycle (disinfection, sample-taking and plating)
• Rotating arm with high speed movement
• Patented electronic robot system
• 10 Petri dishes plated in less than 2 mn (with the same sample)
Overflow technology*
sample
drains away
cleaning with
alcohol
rinsing with
sterile water
• No cross-contamination
• Unique patented disinfection system by overflow
• 600 cleanings without changing bottles (2L)
USB programmable volume & traceability**
• Easy PC connectivity via USB
• From 10 to 1000 microliters programmable volumes
• 3 preset plating modes
• Monitoring software with traceability
XLS
Excel™
USB
Consumables
reduced up to 75%!
• Reduced budget for consumables
• No specific consumables needed
• More space in your laboratory
*Scientific study available upon request
**On easySpiral Pro®
6
30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com
®
Plate & Count System + dataLink™
Plate & Count System® + dataLinkTM offer quick
and reliable results from automatic plating to colony
counting with total traceability.
GREAT SAVINGS: Up to 75 % savings in time,
consumables and space
QUICK: Full plating cycle in 25 seconds and
counting in 1 click. No need of manual data input
as the Scan® colony counter retrieves it and adjusts
automatically.
RELIABLE: Repeatable and reproducible results
up to 98 %
FULL TRACEABILITY WITH
Automatic saving of data and results
dataLinkTM:
easySpiral Pro®
and Scan® 4000
How does it work ?
STEP 1
Plate with easySpiral Pro®.
easySpiral® software collects the plating data.
®
al Pro
easySpir
STEP 2
Print the label with Datamatrix code. Stick the label on the plated
Petri dish and place in the incubator.
… 24-72 h incubation
AUTO
PDF
JPG
JPEG
Scan 4000
Adobe
XLS
Excel™
LIMS
STEP 3
Once the colonies have grown, scan the Datamatrix code. The
Scan® colony counter automatically adjusts its settings thanks to
the Datamatrix label’s data. Click on “COUNT”. Export the data.
* Please check LIMS compatibility
D
DOWNLOAD
S
C ® SOFTWARE
SCAN
www.interscience.com
30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - [email protected] - www.interscience.com
7
easySpiral Pro®
412 000
413 000
Syringe capacity
APPLICATIONS
1000 µL
Preset deposited volume
Counting range
50 or 100 µL
50, 100 or 200 µL
300 to 1.3 x 105 CFU/mL
100 to 1 x 107 CFU/mL
25 seconds
Plating time (50 µL - disinfection + plating)
Overflow technology
• Microbiological analyses
• Food quality control
• Bacterial cinetics
8 bars pressure
Disinfectant autonomy (in normal mode)
1000 cycles (2L bottles)
Distilled water autonomy (in normal mode)
600 cycles (2L bottles)
• MIC of antibiotics
Successive plating capacity with the
same sample
Up to 20 Petri dishes (50 µL)
• Challenge test:
cosmetics control
Automatic disinfection program
✔
✔
• Pharmaceutical control
90 mm Petri dish
✔
✔
-
✔
Exponential deposition mode
✔
✔
Circle deposition mode
✔
✔
Constant deposition mode
-
✔
Excel™ export traceability
-
✔
Programmable deposited volume
(via USB)
-
from 10 to 1000 µL
150 mm Petri dish
Dimensions
40 x 41.5 x 29 cm
Weight
16.4 Kg
Power supply
100-240 V~
50-60 Hz
Delivered with: 1 blue dye for testing, 1 syringe
with silicone tubing, Spiral® counting grids: 90 mm &
150 mm*, support test container, 2 filters, 1 replacement
stylus, 4 connector sets for GL45 bottles, 1 GL45
double connection connector set, 1 pot of EnzyClear®,
1000 sterile beakers, power cord, user manual, 150 mm
Petri dish ring*, software on a CD-ROM*, USB cable*.
*Only for easySpiral Pro®
Accessories
Housing protection for platers
dataLink™
DB 50 beakers
Bar-code reader
Transparent housing for working
outside laminar flow
Ref 413 001
Traceability system
Ref 410 100
1 000 sterile beakers for Spiral® platers
Ref 415 100
Barcode reader for traceability
Ref 522 000
INTERNATIONAL
Your local distributor
3 0, c h e m i n du Bois d e s A r p e nts - 78 8 6 0 S t N o m - FR A NCE
T: +33 (0)1 34 62 62 61 - F: +33 (0)1 34 62 43 03 - Email: [email protected]
USA & CANADA
32 Cu m m in g s Pa r k - Wo bu r n, M A 018 01 - USA
T: +1 781 937 0007 - F: +1 781 937 017 - Email: [email protected]
CHINA
上海市徐汇区吴 兴路277号锦都大 厦5 8 8室 - 20 0 0 3 0
电话: +86 (0)21-64739390 - +86 189 3097 0733 - 邮址: [email protected]
SOUTH-EAST ASIA
www.
interscience.com
5 41 O r c h a r d r o a d - 0 9 - 0 1 L i a t To w e r s - S I N G A P O R E 2 3 8 8 8 1
T: +65 6933 1389 - +65 8549 1217 - Email: [email protected]
3073 easySpiral_B_0216. Pictures and information in this brochure are not contractually binding. Spiral®, easySpiral®, easySpiral Pro®, Spiral Plater®, Plate & Count System®, Scan® are registered trademarks. INTERSCIENCE reserves the right to change or improve the specifications of its products without notice. Please visit
www.interscience.com for current updates and additional information. RCS 950 356 220 Versailles. INTERSCIENCE SARL F78860.
Reference
easySpiral®
EN
Technical specifications
®
easySpiral Dilute
The world's first automatic diluter & plater
A revolution
in serial dilutions!
Our quality for your lab
• Designer and manufacturer for microbiology
• R&D leadership for innovative and reliable products
• Worldwide distribution network in more than 85 countries
interscience
R&D center and manufacturing plant
Mourjou FRANCE
• Made in France
®
Spiral method:
35 years experience
The Spiral® method was designed in 1973 to automate the routine
work of bacterial enumeration by Dr. Ed Campbell, researcher at the
FDA (Food & Drug Administration). With François Jalenques, friend
and founder of interscience, they patented an updated method
in 1992.
Since then Spiral® automatic platers have been a reference for
applications in food microbiology, medical bacteriology, research
on food preservatives or cosmetological factors in compliance
with the NF V08-050, NF V08-034, ISO 7218, ISO 4833-2,
ISO 15189, FDA BAM and AOAC 977.27 standards.
Today interscience is proud to launch the 4th generation of
Spiral® platers with easySpiral Dilute®: the world's first automatic
diluter & plater.
F. Jalenques & Dr. Ed Campbell
1979
Spiral® DS
First Spiral® plater
1996
Spiral® DS+
Automatic Spiral® plater
2010
easySpiral®
World's fastest Spiral® plater
PATENTED
2012
easySpiral Dilute®
World's first automatic diluter & plater
PATENTED
interscience is proud to introduce easySpiral Dilute®, a 2-in-1 automatic diluter and plater, which allows you
to do 5 x 1/10th serial dilutions. It will then automatically plate on 1 single Petri dish, with a countable range from
30 to 1 x 1012 countable CFU/mL.
No more manual dilutions and plating is automatic!
2
Plate
Place sample
1
Dilute
Place your sample in the beaker
1. The easySpiral Dilute® will automatically dilute the sample up to 5 times
2. The easySpiral Dilute® will automatically plate the sample on a Petri dish up to 1 x 1012 countable CFU/mL
Why use an automatic diluter & plater?
easySpiral Dilute® allows you to save 50% on consumables and 50% on your time.
Comparison between manual and automatic method below.
Example of 50 samples: Dilution: 10-7
Plating: 10-5, 10-6 and 10-7
Manual method
Automatic method
CONSUMABLES
CONSUMABLES
Dilution
Dilution
• 400 tubes
• 350 beakers
• 350 pipet tips
Plating
Plating
• 450 Petri dishes
• 150 Petri dishes
TIME
10h10
TIME
5h50
3
Key features
Compact
High precision glass syringe
38 cm wide, can be used inside and outside a laminar flow
Stainless steel housing
Typical accuracy of 0.5 %
4x more precise than a basic pipet
No more pipet calibration
High speed automation
Fast rotating arm
1 disinfection, 5 dilutions and 1 plating in 134 s
PATENTED
Automatic disinfection
One-touch plating
Overflow technology cleaning system
No cross contamination
Choose from 4 plating modes
Plate on 55, 90 and 150 mm Petri dishes
PATENTED
Easy serial dilutions
High capacity
Choose your dilution factor
Do 10-5 dilution or more automatically
No more 9 mL tube preparation
2 fully autoclavable bottles
(disinfectant & diluent) and connectors
PATENTED
4
How does it work?
1. Dilution cycle
1
Sample intake
sample
2
0.5 mL of sample is deposited
sample
10-1
sample
10-1
3
4.5 mL of diluent is added to the sample and mixed
The above dilution cycle is repeated for the next serial dilution.
0.5 mL of 10-1 diluted sample intake
1
sample
10-1
sample
10-1
2
0.5 mL of 10-1 diluted sample is deposited
10-2
Dilution cycle can be repeated as many times as you wish.
2. Plating cycle
Constant mode
Exponential mode
Circle mode
Pour mode
Homogenous
surface deposition
Decreasing surface
concentration
3 or 6 surface dilutions
on 1 Petri dish
Homogenous deposition*
in pour plate mode
50 µL, 100 µL, 200 µL
and USB programmable
50 µL, 100 µL, 200 µL
and USB programmable
10 µL inner circle, 16 µL middle circle
and 25 µL outer circle
50 µL, 100 µL, 200 µL
and USB programmable
Examples:
Before
incubation
Homogenous surface deposition
+ concentrated
- concentrated
10-1 10-2 10-3
Homogenous deposition
in pour plate mode*
After
incubation
Easy colony counting:
No colonies on the edge
of the Petri dish
Counting on Petri dish
up to 1 x 1012 CFU/mL
Savings
on Petri dishes
Easy colony counting
for invasive colonies.
Pharmacopoeia compliant
* In pour mode, easySpiral Dilute® deposits one sample drop at the bottom of an empty Petri dish. Then, the user adds the molten agar on top and homogenizes it with the sample.
How to count?
Manual colony counting
Automatic colony counting
Scan® 100
or
with Spiral®
counting grid
Scan® 300
Scan® 500
Scan® 1200
Scan® 4000
5
Questions
What is special about
the easySpiral Dilute®?
easySpiral Dilute® is the only 2-in-1 equipment
on the market making automatic serial dilutions and standardized plating.
The accuracy of
easySpiral Dilute® is high: 0.5%.
How can I check it?
It is easy and automatic.
Test of the deposited volume during plating:
Press on the plating volume test button. This
function will fill a previously weighed beaker
with 500 µL. Weigh the distributed volume to
check the results.
Test of the volume of the dilutions:
Press on the dilution volumes test button.
This function will fill 2 previously weighed
beakers: one with 0.5 mL and the 2nd one
with 4.5 mL. Weigh the distributed volumes
to check the results.
Which diluents can I use?
easySpiral Dilute® works with saline solutions (e.g.: Ringer's solution, physiological
saline).
Avoid using diluents with nutrients as they may
cause contaminations. Bacteria only remain a
few seconds in the beakers before being deposited on the Petri dish so using a saline diluent has no effect on the final enumeration.
It is however possible to use nutrient solutions
as diluent in the semi-automatic "My Diluent"
mode. In that case, fill in the beakers with
4.5 mL diluent before processing.
6
I usually dilute 1 mL to 1/10
and the easySpiral Dilute®
uses 0.5 mL to 1/10.
Is easySpiral Dilute® as precise
with a volume divided by 2?
Usually the average accuracy for the 9 mL
tubes filling and 1 mL pipetting is 2% with the
need to calibrate and regularly check the pipets you are using.
easySpiral Dilute® has an average accuracy of
0.5% on 0.5 mL and 4.5 mL volumes because
it works with a high precision HamiltonTM glass
syringe in a liquid displacement circuit.
With volumes divided by 2, precision and
repeatability are then much higher!
I need to make a 10-7 dilution.
How can I do it?
The easySpiral Dilute® can do as many
dilutions as you wish.
Do a 10-5 dilution, take the last beaker and
place it as the mother sample and then
proceed with a new automatic dilution
sequence.
Accessories & technical specifications
Housing protection
dataLinkTM
Beakers DB 50
Barcode reader
Transparent housing for working
outside laminar flow.
Traceability system
1 000 sterile beakers for Spiral®
platers (5 mL)
Barcode reader for traceability
Ref. 413 001
Ref. 410 100
Ref. 415 100
Ref. 522 000
easySpiral Dilute® is delivered with 1 000 sterile beakers, 1 blue dye for tests, 1 box of EnzyClear®, 4 sets of GL 45 bottle connectors, Petri dish rings: 55, 90 & 150 mm,
Spiral® counting grids: 90 & 150 mm, circle counting grids: 90 & 150 mm, power cord, user’s manual, monitoring CD-ROM software, USB cable.
Reference
Petri dish diameter
Syringe capacity
Programmable dispensed volume (via USB)
Preset dispensed volume
Detection in CFU/mL of Spiral® plating
Plating modes
Number of dilutions at 1/10th for 1 cycle
1 disinfection + 5 dilutions + 1 plating of 1 dilution
1 disinfection + 5 dilutions + 1 plating of each dilution
Disinfectant autonomy
Diluent autonomy
Circle mode (patented)
Stylus disinfection system (patented)
Pressure in the stylus
Successive plating capacity with the same sample
Traceability
Monthly cleaning program
Filling time programmable via USB
My Diluent key
Sample mixing before dilution/plating
Controlled by microprocessor
Dimensions (w x d x h)
Weight
Power supply
Power
1 year guarantee (after registration)
All stainless steel
Made in France
NF
NF
ISO
ISO
ISO
V08-050
V08-034
7218
4833-2
15189
easySpiral Dilute®
414 000
55 mm, 90 mm and 150 mm
1 000 µL
from 10 µL to 1 000 µL
50 µL, 100 µL and 200 µL
30 to 1 x 1012 CFU/mL
55 mm: constant, pour plate
90 mm: exponential (10-5), constant, circle (3 dilutions), pour plate
150 mm: exponential (10-7), constant, circle (6 dilutions), pour plate
5
134 s
234 s
1000 (with 2 L bottles)
63 cycles (with 2 L bottles)
3 dilutions on a 90 mm Petri dish
6 dilutions on a 150 mm Petri dish
inside and outside by Overflow technology
8 bars
up to 20 Petri dishes (50 µL)
excelTM, datamatrix labels
D
D
D
D
D
39 x 42 x 29 cm
15.6 kg
100-240 V~ / 50 Hz to 60 Hz
65 W
D
D
D
FDA BAM
Bacteriological Analytical
Manual
AOAC
977.27
ISO
9001
2008
WEEE
RoHS
2002/96/EC
2002/95/EC
MADE IN
FRANCE
7
USA & CANADA
199 Weymouth ST. - ROCKLAND - MA 02370 - USA
T: +1 781 792 2133 - F: +1 781 792 2134
[email protected] - www.intersciencelab.com
CHINA
上海市徐汇区吴兴路277号锦都大厦718室-200030
电话: +86 (0)21-64739390 - 手机: +86 189 3097 0733
[email protected] - www.interscience.fr/china
Distributor
www.
interscience .fr
easySpiral Dilute_B_0615. Pictures and information in this brochure are not contractually binding. Spiral®, easySpiral®, easySpiral Dilute®, Scan® are trademarks. This document is the translation of the french brochure and is not contractually binding. INTERSCIENCE reserves the right to change or improve the specifications of its
products without notice. Please visit www.interscience.fr for current updates and additional information. RCS 950 356 220 Versailles. INTERSCIENCE SARL F78860.
30, ch. Bois Arpents - 78860 St Nom - FRANCE
T : +33 (0)1 34 62 62 61 - F : +33 (0)1 34 62 43 03
i n f o @ i n t e r s c i e n c e .f r - w w w. i n t e r s c i e n c e .f r
EN
INTERNATIONAL
Appendix 2. easySpiral® / easySpiral® Pro validation studies
1. Spiral plating method validation
Purpose
To study the reliability of Spiral plating with easySpiral® comparing to the reference method
(classical surface plating).
Equipment and methods
Five bacterial strains (three gram+ and two gram-) are tested: Lactobacillus casei var.
rhamnosus, Bacillus cereus (ATCC 11778), Bacillus subtilis (ATCC 6633), Escherichia coli
(ATCC 8739) and Pseudomonas aeruginosa (ATTC 9027).
Bacterial cultures are diluted in buffered peptone water then plated on Petri dish agar (90
mm), using classical method and Spiral method (50!L in exponential mode).
After 48h of incubation at 37 C° (±1) on MRS (De Man, Rogosa, Sharpe) for Lactobacillus
casei and 18h (±2) at 37C° (±1) on PCA (Plate Count Agar) for Bacillus cereus, Bacillus
subtilis, Pseudomonas aeruginosa and on Macconkey for Escherichia coli, counting is made
with an automatic colony counter (Scan®1200).
Results analysis
Counting results are expressed in Log CFU/mL (Colony Forming Unit) and a regression
analysis is made to assess the correlation between, Spiral method with easySpiral® Pro and
classical plating method.
Results
- Bacillus cereus:
Spiral method (Log CFU/mL)
5
y = 0,974x + 0,0217
R2 = 0,982
4.5
4
3.5
3
2.5
2.5
3
3.5
4
4.5
5
Classical method (Log CFU/mL)
Figure 1: Correlation between Bacillus cereus counting (Log CFU/mL) obtained by plating in classical
and Spiral method.
85/125
®
®
easySpiral / easySpiral Pro : Guide d’installation et d’utilisation V3
- Bacillus subtilis:
5
Spiral method (Log CFU/mL)
y = 0.9923x + 0.0238
2
R = 0.9853
4.5
4
3.5
3
2.5
2.5
3
3.5
4
4.5
5
Classical method (Log CFU/mL)
Figure 2: Correlation between Bacillus subtilis counting (en Log CFU/mL) obtained by plating in
classical and Spiral method.
- Lactobacillus casei var. rhamnosus:
Spiral method (Log CFU/mL)
5.5
y = 0,9036x + 0,335
2
R = 0,994
5
4.5
4
3.5
3
3
3.5
4
4.5
5
5.5
Classical method (Log CFU/mL)
Figure 3: Correlation between Lactobacillus casei var. rhamnosus counting (en Log CFU/mL)
obtained by plating in classical and Spiral method.
®
®
easySpiral / easySpiral Pro: Installation guide and user’s manual V3
86/125
- Escherichia coli:
Spiral method (Log CFU/mL)
5.5
y = 0,9822x + 0,0853
R2 = 0,989
5
4.5
4
3.5
3
3
3.5
4
4.5
5
5.5
Classical method (Log CFU/mL)
Figure 4: Correlation between Escherichia coli counting (en Log CFU/mL) obtained by plating in
classical and Spiral method.
- Pseudomonas aeruginosa:
Spiral method (Log CFU/mL)
5
y = 0,9632x + 0,1039
R2 = 0,990
4.5
4
3.5
3
2.5
2
2
2.5
3
3.5
4
4.5
5
Classical method (Log CFU/mL)
Figure 5: Correlation between Pseudomonas aeruginosa counting (en Log CFU/mL) obtained by
®
plating in classic and easySpiral pro method.
Conclusion
Results show a strong correlation (R2 " 0.982) between Spiral method with easySpiral® Pro
and Classical method. Hence the average difference of CFU/mL log between the two
methods is resulting to be not significatif 12 times lower than the maximal deviation (0.5 log)
authorised by AFNOR NF V08/100.
87/125
®
®
easySpiral / easySpiral Pro : Guide d’installation et d’utilisation V3