Automated GC fractionation for high resolution fraction
collection of complete GC chromatograms with parallel
mass spectrometric detection
7
Jeroen Kool
[email protected]
Division of BioAnalytical Chemistry
Group of BioMolecular analysis
VU University Amsterdam, The Netherlands
Complex Bioactive Mixtures
Combinatorial Libraries/
Parallel synthetic libraries
Nature
Metabolic mixtures
Reactive
metabolite
Bioactive
metabolite
Parent
compound
CYP inhibiting
metabolite
Inactive
metabolite
And of course environmental mixtures
Traditionally assessed with Effect Directed Analysis
for correlation of Bioactives to their Identity
Or for example the oil and chemical industry
Efficient fractionation strategies are always in
demand in these industries for analysis
Or any other mixture…..
How to purify compounds to be
studied further
First, separation followed by
fractionation is needed
LC
GC?
Why is GC almost never used?
Intrinsic drawbacks of
Fractionation using LC
Example:
Especially in environmental analysis, for many
pollutant classes, GC is by far the preferred (and
certified) separation technology
But because of many difficulties related to GC
fractionation still LC fractionation is the preferred
separation strategy
Solution: New GC fractionation technique
GC fractionation:
How did we test the system?
Column
GC Separation
FID
Injector
MS or FID
Injector
Column
Carrier solvent
GC Separation
GC fractionation:
How did we test the system?
How does it work
Inject C7-C30
mix
Do we collect
them?
Are they still
separated?
GC fractionation:
Data Analysis
Fraction
#
GC fractionation:
applications
MS or FID
Injector
?
Column
GC Separation
Carrier solvent
GC fractionation:
applications
Repeated fractionations, collection in the same well plate
B
C
Fractionation
10x to 100x
Analysis by GCFID or bioassay
or …
Contains 10 to 100 fraction collection cycles
Example on next slide
The New Setup:
The GERSTEL-XYZ sampler from Da Vinci
Acknowledgements
BioMolecular Analysis
Institute for Environmental Studies
Willem Jonker
Govert Somsen
Martin van Velzen
Jorke Kamstra
Pim Leonards
Timo Hamers
Marja Lamoree
Da Vinci Laboratory
Solutions B.V.
Jaap Schaap
Sjaak de Koning