EasiVial Polystyrene PS-L
(2 mL) Standards for GPC
Contents
This kit contains ten of each color-coded vial (red, yellow and
green). Each vial contains a mixture of four narrow polydispersity
polystyrene standards (Part No. PL2010-0401). The molecular
weights and masses of the polymers contained in each vial are
shown on the lot-specific Certificate of Analysis accompanying this
pack. As supplied in the EasiVial format, the polystyrene standards
are stable and do not require any special storage conditions. Also
available in 4 mL vial (Part No. PL2010-0400) and Tri-Pack (90 vial)
formats (Part No. PL2010-0402).
Preparation for use
Up to 2 mL of solvent can be added to each vial, the volume of
solvent added can be chosen to give the required final polymer
concentration appropriate for the application. After addition of the
solvent, the polymers should be allowed to dissolve for at least one
hour and the vial should be shaken gently to completely disperse
the polymer solution prior to use. Once prepared in dilute solution,
polystyrene standards may be susceptible to degradation and
EasiVials containing the solutions should be stored in a cool, dark
place and used within one week.
For molecular weight (Mp) values for this kit, please refer to the lotspecific Certificate of Analysis included in this pack.
Application
EasiVial PS-L standards can be used for conventional GPC calibration
employing a single concentration detector or for Universal
Calibration using GPC-viscometry. The concentration at which the
standards are prepared will depend on the dn/dc for the solvent
system in use and the relative sensitivity of the detectors employed.
As a guide, for use with THF, where the dn/dc=0.185 mL/g, it is
recommended that 1 mL of solvent is added to the vials whereas for
use with TCB, where the dn/dc=0.053 mL/g, it is recommended that
0.5 mL of solvent be added to the vials. Please note that these vials
are not suitable for a PL-GPC 220 at high temperature.
Recommended Conditions
Columns:
PLgel 3 µm MIXED-E, 300x7.5mm
Eluent:
THF
Flow rate:
1.0 mL/min
Detector:
DRI
Inj vol:
100 µL
Sample prep:
1 mL solvent added to vials
Figure 1. Typical Chromatograms
Figure 2. Typical GPC Calibration Data
Learn more:
www.agilent.com/chem/gpcsec
Buy online:
www.agilent.com/chem/store
Find an Agilent office or authorized distributor:
www.agilent.com/chem/contactus
U.S. and Canada
1-800-227-9770, [email protected]
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India
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© Agilent Technologies, Inc. 2011
Published in the UK, February 28, 2011
Part Number PKEVPS-L2