Technical Note
TD007EN Ver1.
Cellufine A-500
Cellufine A-500 can be used at high flow rates.
Lin ear flow veloc ity [ c m/h ]
35 0
A5 0 0 I.D.9 cm- 35 cm
30 0
A g a ros e ba s e
A E X-g el
I..D.9cm -35cm
25 0
A5 00 I.D.2 5c m-2 5c m
The flow/pressure curve for a Cellufine A-500
column confirms operating flow rates above
250 cm/h can be obtained at column size
I.D.25cm.
20 0
A g a ros e ba s e
A EX-g el
I..D .25cm -25cm
15 0
10 0
50
0
0
0.1
0 .2
Prssu re [ MPa ]
0 .3
0 .4
Fig1.Pressure-flow rate curve for Cellufine A-500.
Column size : indicated on the figure
Mobile phase :0.01M phosphate buffer,pH7.2 at 25℃
Cellufine A-500 is stable in alkali /acid
Relative IEC ( % )
80
60
40
A
20
Relative capacity,BSA ( % )
100
100
80
60
40
B
20
Fig2. Stability test in alkali and acid at 4℃/20℃.
0
0
60
120
180
day
0
0
4℃/0.2M HCl
4℃/0.5M NaOH
20℃/0.2M HCl
20℃/0.5M NaOH
60
da y
120
180
4℃/0.2M HCl
4℃/0.5M NaOH
20℃/0.2M HCl
20℃/0.5M NaOH
Figure A: affection of ion exchange capacity ( IEC )
Figure B: affection of the adsorption capacity of bovine serum albumin( BSA ).
When stored in 0.2M HCl and 0.5M NaOH for 180 days at 4℃and 20℃, both the adsorption capacity and IEC are
stable.
The graphs show relative comparison to virgin product (as 100%).
TD007EN Ver1.2 (April 1, 2011)
1
Relative capacity,BSA [ % ]
Technical Note
TD007EN Ver1.1
Cellufine A-500
performance remains
constantly at least 100
operating cycles.
100
80
60
40
0.2M NaOH
20
The data to the left shows 100 Cleaning in
Place (CIP) cycles followed by cleaning in
NaOH or HCl. The adsorption capacity of
BSA does not change before and after 50
times and 100 times cleaning.
0.5M NaOH
0.2M HCl
0
0
50
Cycle
100
Fig4. Stability by repeat cleaning (CIP) of Cellufine A-500.
column : I.D.4.4cm-bead height 25cm Flow rate : 38cm/hr at 20℃
buffer : 0.01M sodium phosphate , pH7.2
regeneration solution : 0.2M NaOH or 0.5M NaOH or 0.2M HCl
Cycle 1)buffer 3CV ;2)regeneration solution 3CV
 Cellufine A-500 is autoclaveable.
 It is recommended that autoclaving at 121°C 20 min. in
a neutral, low salt buffer.
 Cellufine A-500 conforms to USP28, Plastic Class V.
2-1, Otemachi 2-chome, Chiyoda-ku
Tokyo 100-8105 Japan
[email protected]
TD007EN Ver1.2 (April 1, 2011)
2