Test for Diffusion, Osmosis and Dialysis activity Name____________________ Teacher _________________________ Date________________________ Class ________________________ 1. A cell was placed in distilled water. What will happen to the cell over time if a cellular membrane is permeable to water but not to the salt ions? Draw a picture below. A cell was placed in a highly concentrated solution of table salt. What will happen next to the cell over time if its membrane is permeable to water but not to salt? Draw a picture. Water Salt Cell Membrane Permeable to water but not to salt 1 A B 2. In figure A there are two water reservoirs connected by a semi-permeable membrane that lets water through it but does not let salt ions go through it. Some salt was added to one of the reservoirs, and the result was (Fig B). Explain, where the salt was added and why the water level changed? 2 3. When blood passes through the kidneys, small molecules of waste are removed from the blood, while large molecules, such as proteins, and red blood cells remain. When kidneys do not work well, people need to be on hemodialysis, using a set up similar to the one in the picture to the left. Blood goes go through cellophane tubings surrounded by a large volume of moving fluid that takes wastes away. Cellophane tubings Waste product such as urea .. Red Blood cells .. .... Pores in the tubings .. .. White Blood cells Proteins .. .. Dialysis Fluid This is a close up of the cellophane tubing with blood in it. The dialysis fluid surrounds the tubing. 3a. What size should the pores in the tubing be compared with the size of waste and other components of blood shown in the illustration? .. .. .. 3b. Suppose the dialysis liquid is not renewed but is recirculated again and again. Describe what would happen. 3 4. A lab technician needs to remove molecules of urea (white dots) from a protein solution in order to restore the protein’s structure (urea was used as a denaturation agent). To do so, she placed the sample in a dialysis bag and placed the bag in a large jar of water. 4a. She made sure the volume of the surrounding water was much greater than the volume of the bag. Why did she do need to do this? 4b. She kept replacing the water in the jar. Why? 4
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