Faecal Calprotectin home test in Ulcerative Colitis
- is it possible?
Margarita Elkjaer1, Johan Burisch1, Arne Røseth2, Vibeke Voxen Hansen1, Birgit Deibjerg Kristensen1,
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Dag Bremnes , Jens-Kristian Slott Jensen , Pia Munkholm
1
Herlev University Hospital, Copenhagen, Denmark
2
Aker University hospital, Oslo Norway
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Skannex AS, Oslo, Norway
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Slott Stat, Bagsværd
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Background
• Enzyme-linked immunosorbent assay
(ELISA) is a quantitative method & the
“Gold standard” for faecal Calprotectin
(FC) analysis
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Results
Figure 1. Comparison of RTSCAN vs. ELISA
of measuring faecal Calprotein (mg/kg)
• 175 faecal samples have been collected
from ulcerative colitis (UC) patients involved in a randomised control trial “Constant-care”
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• ELISA vs. RTSCAN mean difference of
5.1 mg/kg (95% CI; ÷16.5-26.6) fig 1
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ELISA
• A new quantitative method, the ”Rapid
Test” (RT), has been developed to be
carried out by the lab technician, in order
to have the results the same day as the
faeces sample is received.
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• ELISA vs. HTPHOTO mean difference of
26.7 mg/kg (95% CI; 1.3-52.2) fig 2
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• RT has further been developed into a
“Home Test” (HT) to be carried out by the
patients themselves
• To compare the ELISA with the RT´s for
FC measurement
- read by a PC-scanner system
- using an ordinary cell phone system
with special software built in (DB)
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RTSCAN
Figure 2. Comparison of HTPHOTO vs. ELISA
of measuring faecal Calprotein (mg/kg)
• The coefficient of variation (CV %) of ELISA was in median 2.8%
(range, 0-21.6%) vs. HTPHOTO 6.2%
(range, 0-41.6%), p-value <0.01
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Conclusion
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ELISA
Aim
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• RTSCAN vs. HTPHOTO mean difference
of 21.7 mg/kg (95% CI; 11.5-32.0) fig 3
4000
A positive correlation between ELISA vs.
Home Test was revealed with the CV is
being less than 10%.
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HTPHOTO
Methods
In this study the RT including HT was carried
out by our lab technicians.
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HTPHOTO
Grafik & TV • grafi[email protected] • 14021
• The colour intensity of the test line was
read after 10 min incubation time
- using a lap-top linked with an ordinary
table top scanner (photo 1 and 2)
- using a cell phone by taking & sending
a picture to a server in Oslo by using
Mobile Internet (photo 3)
Figure 3. Comparison of HTPHOTO vs. RTSCAN
of measuring faecal Calprotein (mg/kg)
RTSCAN
• After dilution one drop of faecal extract
was applied onto the RT- device
In future aspects the Home Test can be implemented in UC patients as part of disease
self-management.