Sartobind® 75 mL, 4 mm and 150 mL, 8 mm bed height
Void-volume-optimized membrane
chromatography capsules for pilot scale
Benefits
– Applicable for flow-through polishing including
bind & elute applications
– Increased dynamic binding capacity at 10% breakthrough
– Small elution volumes compared to non-optimized capsules
– Plug and play pilot scale device
– Disposable or reusable
Product Information
Sartobind 75 and 150 mL capsules are intermediate sizes of void-volume optimized capsules with 4 and 8 mm
membrane bed height. For the capture of large proteins or viruses it is important to achieve a steep breakthrough
curve and have the smallest possible elution volume. The optimized construction of the capsule makes it ideal
for both removing contaminants | impurities via flow-through polishing and for bind and elute applications.
The SingleSep® product line has been a standard for flow-through applications, but these capsules are not
void-volume-optimized. The new 75 mL version is optimized and will replace the 5” SingleSep® 70 mL capsule
(see fig. 1a) in flow-through applications as the membrane type and bed height are unchanged. This allows flow
rate and binding capacity to be scaled in the same way across the product line.
75 mL
0.08
1
7
70
180
350
Fig. 1a: Sartobind 4 mm bed height portfolio.
The 75 mL will replace the 5 inch 70 mL capsule.
540
1620 mL
3
150
1,200
5,000 mL
Fig. 1b: The current scale of capsules with 8 mm bed height
(150 mL capsule shown in expanded view in figure 2).
Membrane Chromatography
The 75 and the 150 mL membrane bed volumes are integrated into
housings of the same size. The bed height of the 75 mL capsule is
half that of the 150 mL version (Fig. 2).
Comparison of a standard capsule originally derived from filter
housings without optimization to a void-volume optimized 1 L
capsule shows that the dynamic binding capacity at 10% breakthrough can be increased by almost 50%. The difference in void
volume is 2.4 liters (–63%); 3.8 L (non-optimized) versus 1.4 L
­(optimized). The binding capacity at 10% breakthrough is improved
from 18.2 mg/mL (non-optimized) to 26.9 mg/L in the optimized
version (Fig. 4). The sample used was 2 g/L bovine serum albumin
in 20 mM Tris pH 7.4 at a flow rate of 5 L/min.
1.0
0.9
0.8
0.7
c/c0
0.6
0.5
0.4
0.3
0.2
0.1
Fig. 2: Cut away of the capsule with 4 mm bed height (75 mL) left and that
with 8 mm (150 mL) right.
The major difference between void-volume-optimized and
non-optimized construction is displayed in figure 3. The internal
void volume of the Sartobind SingleSep® capsule is 320 mL, while
that of the optimized 75 mL capsule is only 180 mL representing
a reduction of 44%.
0.0
0
20
40
Load [mg/mL]
60
80
Optimized 1 L capsule
(8 mm)
Standard 1 L capsule
Standard 1 L capsule
Optimized 1 L capsule Fig. 4: BSA breakthrough curves of optimized and standard non–optimized
1 L capsules with 8 mm bed height.
The dynamic binding capacity at 10% breakthrough is increased
by 17% for the 75 mL capsule compared to the non-optimized
70 mL (Fig. 5). Sample used was 2 g/L BSA in phosphate buffer at
pH 7.0 at a flow rate of 280 to 350 mL/min.
1.2
1.0
0.8
c/c0
1.2
c/c0
1.0
0.6
0.8
0.4
0.6
0.2
0.4
0.0
Fig. 3: The void-volume optimized capsule with 4 mm bed (75 mL, left) compared
to the SingleSep® 5” (700.2mL) without void- volume optimization.
0.0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
Load [MV]
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
Load [MV]
Standard Q 5" 70 mL capsule
Optimized Q 75 mL capsule
Fig. 5: BBSA breakthrough curves of optimized 75 mL and standard
non-optimized 70 mL capsules with 4 mm bed height.
Standard Q 5" 70 mL
Optimized Q 75 mL ca
Technical Data
Specifications
Bed height
Base membrane
Membrane thickness
Nominal pore size
Bed height
Bed volume
Adsorption area
Void volume
Void volume in membrane volumes
Recommended flow rate
4 mm
Stabilized reinforced cellulose
275 µm
> 3 µm
4 mm
75 mL
2700 cm2
0.18 L
2.4
1.5 L
8 mm
Stabilized reinforced cellulose
275 µm
> 3 µm
8 mm
150 mL
5500 cm2
0.2 L
1.3
0.75 L
Dimensions (height + diameter)
triclamp version
190 + 77 mm
190 + 77 mm
Dimensions (height + diameter)
hose barb version
203 + 77 mm
–
Membrane types and ligands
Strong basic anion exchanger
Strong acidic cation exchanger
Hydrophobic interaction membrane
Binding capacity* mg per mL | g/75 mL and g/150 mL capsule
Q
S
Phenyl
Housing material
Connectors
Max. Pressure
Weight
Short term pH compatibility
Chemical stability
Quaternary ammonium (Q)
(R-CH2-N+-(CH3)3)
Sulfonic acid (S)
(R-CH2-SO3-)
Phenyl
(-C6H6)
29 | 2.16 | 4.4
25 | 1.89 | 3.9
14.5 | -** | 2
Polypropylene
Sanitary 25 mm ¾” | hose barb***
4 bar (0.4 MPa, 58 psi)
400 g
Q: 2 – 14
S: 3 – 14
Phenyl: 2 – 14
Stable in common chromatography buffers, unstable to peroxide
and other oxidizing or reactive reagents
* Reference proteins: Bovine serum albumin for Q, lysozyme for S and bovine IgG for Phenyl
**not available
*** only for Q and S 75 mL
Membrane Chromatography
3
Ordering Information
Article No.
Sartobind Q capsules
96IEXQ42D9MOO--A
96IEXQ42D9MFF--A
96IEXQ42E9BFF
Description
Quantity
Sartobind Q 75 mL, 4 mm bed height, hose barb connectors
Sartobind Q 75 mL, 4 mm bed height, sanitary clamp connectors ¾”
Sartobind Q 150 mL, 8 mm bed height, sanitary clamp connectors ¾”
4
4
1
Sartobind S capsules
96IEXS42D9MOO--A
96IEXS42D9MFF--A
96IEXS42E9BFF
Sartobind S 75 mL, 4 mm bed height, hose barb connectors
Sartobind S 75 mL, 4 mm bed height, sanitary clamp connectors ¾”
Sartobind S 150 mL, 8 mm bed height, sanitary clamp connectors ¾”
4
4
1
Sartobind Phenyl capsule
96HICP42E9BFF
Sartobind Phenyl 150 mL, 8 mm bed height, sanitary clamp connectors ¾”
1
Enclosed in each package: 1 manual, 1 certificate
Article No.
Accessories
1ZAOGV0003
Description
Quantity
Adapter Sanitary 25 mm 1 – ¾” to UNF 10-32 to connect 75 | 150 mL capsules
to LC systems
2
7ZAL-V0013
Reducing adapter 1 1 – ¾”; 50.5 mm to 25 mm, sanitary
2
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
37079 Goettingen, Germany
Phone +49.551.308.0
Fax +49.551.308.3289
www.sartorius-stedim.com
USA Toll-Free +1.800.368.7178
UK +44.1372.737159
France +33.442.845600
Italy +39.055.63.40.41
Spain +34.90.2110935
Russian Federation +7.812.327.5.327
Japan +81.3.4331.4300
China +86.21.68782300
Specifications subject to change
®
®
without notice.
Printed and
copyrighted
Sartobind
and SingleSep
are
trademarks
by Sartorius
Sartorius Stedim
Stedim Biotech
Biotech GmbH
GmbH. | W
of
Publication No.:
Order No.:
Specifications
subject to change
Ver. 01 | notice.
2013 Printed and copyrighted
without
by Sartorius Stedim Biotech GmbH. | W
First published:
August 15, 2014
Publication No.: SL-2086-e150703
Order No.: 85037-546-56
Ver. 07 | 2015