DNA Ladder Marker
200bp Step Ladder Marker, Ready-to-use
For research use only
Product
200bp Step Ladder Marker,
Concentrated
Volume
Cat. No.
500 ml (100 mg)
EBM-1102
Remarks
ready-to-use
Description
The 200bp step ladder marker is a mixture of double-stranded DNA fragments from 200 to 3,000 bp
in 200 bp step increment. For easy size reference on the gel electrophoresis, the 600 bp, 2,000 bp,
and 3,000 bp are made to be more brighter than the other bands. The 200bp step ladder marker is
supplied in a ready-to-use format. This ladder marker can be stained with ethidium bromide or any
other known DNA staining methods.
Storage Buffer
• Marker DNA : 100 mg in 0.5 ml of 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005%
Bromophenol Blue, and 0.005% Xylene Cyanol
Recommended Storage Condition
• -20℃ for 2 year
• 4℃ for 6 months
• Room temperature (20-25℃) for 2 months
Usage Information
• Concentration : 1,000 ng/5 ml (100 mg/0.5 ml)
• Recommended loading : 2-5 ml (100-200 lanes, ready-to-use)
• Range : 200 bp step increase to 3 kbp
200bp Step
Ladder Marker
bp
3,000
200bp
increase
2,000
1,800
1,600
1,400
1,200
1,000
800
600
400
200
5 ml/1,000 ng/lane ;
1% agarose in 0.5x TAE,
stained with ethidium bromide
Technical Services:
e-mail : [email protected]
www.elpisbio.com
㈜ 엘피스바이오텍
ELPIS BIOTECH
DNA Ladder Marker
Migration Patterns of DNA Ladder Markers in Different % of Agarose Gels
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
1. 100bp DNA Ladder Marker (EBM-1001)
2. 1Kbp DNA Ladder Marker (EBM-1002)
3. 1Kbp Plus 100bp DNA Ladder Marker (EBM-1003)
4. 50bp DNA Ladder Marker (EBM-1004)
5. 1Kbp DNA Mass Ladder Marker (EBM-1009)
6. 100bp DNA Mass Ladder Marker (EBM-1010)
7. 500bp Step Ladder Marker (EBM-1101)
8. 200bp Step Ladder Marker (EBM-1102)
0.7%
25 min
35 min
40 min
25 min
35 min
50 min
25 min
40 min
55 min
0.7%, 1%, 1.5%
0.5x TBE Gel
100V constant
EtBr staining
1%
1.5%
0.7%
1
2
3
25 min
1
2
1%
3
35 min
1
2
3
45 min
1
2
3
25 min
1
2
1.5%
3
35 min
1
2
3
45 min
1
2
3
25 min
1
2
3
35 min
1
2
3
45 min
1. PCR Marker 0.1-1Kbp (EBM-1005)
2. PCR Marker 0.1-3Kbp (EBM-1006)
3. PCR Marker plus 50bp (EBM-1007)
0.7%, 1%, 1.5%
0.5x TBE Gel
100V constant
EtBr staining
1
2
3
25 min
4
5
6
1
2
35 min
3
4
5
6
1
2
3
4
5
6
40 min
1. 1Kbp DNA Mass Marker (EBM-1009)
2. 100bp DNA Mass Ladder Marker (EBM-1010)
3. 500bp Step Ladder Marker (EBM-1101)
4. 200bp Step Ladder Marker (EBM-1102)
5. 100bp Step Ladder Marker (EBM-1103)
6. 50bp Step Ladder Marker (EBM-1104)
1.5%, 0.5x TBE Gel
100V constant
EtBr staining
Technical Services:
e-mail : [email protected]
www.elpisbio.com
㈜ 엘피스바이오텍
ELPIS BIOTECH