silver bullets bio
User Guide
Applications
• Additive screen for the optimization of protein crystals
• Additive screen to discover different crystal forms
• Secondary or orthogonal crystallization screen when traditional
screens are not successful
• Additive screen for the optimization of protein solubility and stability
Features
Screens a portfolio of small molecules for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic
interactions which could promote stability, lattice formation, and crystallization.1-3
• Small organic molecules, organic salts, and organic acids
• Biologically active molecules, co-factors, and ligands
• Amino acids, peptides, nucleotides, drugs, and carbohydrates
• Biochemical pathway intermediates
Silver Bullets Bio is a library of small molecules that have been shown to
promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
• Stabilize the conformation of the protein
• Perturb the interaction of the protein with the solvent
• Participate in forming important lattice contacts
• Build the crystal lattice by forming reversible cross-links between the
macromolecules in the crystal
Published results with the Silver Bullets Bio have been very encouraging,
with more than twice as many proteins being crystallized overall as were
crystallized from controls free of any small molecules.1-3 X-ray diffraction
analysis has revealed the small molecule Silver Bullets Bio in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic
interaction.1-3
Silver Bullets Bio is compatible with hanging, sitting and sandwich drop
vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets Bio can be used with Dynamic Light Scattering
(DLS), ThermoFluor, and Size Exclusion Chromatography assays.
General Description
Silver Bullets Bio is composed of 96 solutions in a single Deep Well block
(Greiner 780261) HT format. Each reagent is a mixture of small molecules
or macromolecular digest in 0.02 M HEPES sodium pH 6.8 buffer. Each
Silver Bullets Bio solution is supplied in a 0.5 ml volume. Each solution
contains between 2 and 20 small molecules. Supplied with two pieces of
AlumaSealTM II Sealing Film (HR8-069) and documentation.
Sample Preparation for Crystallization
The macromolecular sample should be homogenous, as pure as practically
possible (> 95%) and free of amorphous and particulate material. Remove
amorphous material by centrifugation or micro-filtration prior to use.
TM
Solutions for Crystal Growth
HR2-088 (page 1)
The recommended sample concentration is 5 to 25 mg/ml in dilute buffer
(10 to 25 mM). The sample should be free of any unnecessary additives,
other than those essential for sample solubility, stability or activity, to best
observe the effects of the Silver Bullets Bio.
Using Silver Bullets Bio as a Crystallization Additive Screen
The following describes the use of the Silver Bullets Bio with the Sitting
Drop Vapor Diffusion method and a 100 microliter reagent well volume in
a single 96 well plate. Other methods such as Hanging Drop Vapor Diffusion
and Microbatch may also be utilized along with smaller reservoir and drop
volumes.
• Reagent Well
A. Pipet 10 ml of the Silver Bullets Bio reagent into the reagent well
B. Pipet 90 ml of crystallization reagent into the reagent well
• Drop
A. Pipet 200 nl of sample into the sample well
B. Pipet 200 nl of the crystallization reagent/Silver Bullet Bio mixture
from the reagent well into the sample drop
C. Repeat for the remaining Silver Bullets Bio
D. Seal the plate
Alternatively, Silver Bullets Bio can be added directly to the drop and not
added to the reagent well.
• Reagent Well
A. Pipet crystallization reagent into the reagent well
• Drop
A. Pipet 1 ml of sample plus 1 ml of Silver Bullets Bio plus 1 ml of
crystallization reagent
B. Repeat for the remaining Silver Bullets Bio
C. Seal the plate
Using Silver Bullets Bio as an Orthoganol Screen
Set the experiment as described for an Additive Screen but use 25% w/v Polyethylene glycol 3,350 for a polymer biased screen or 55% v/v TacsimateTM pH
7.0 for a salt biased screen. Or choose one or more of the reagents from a
primary crystallization screen that has produced promising results.
Examine the Drop
Carefully examine the drops under a stereo microscope (10 to 100x magnification) immediately after setting up the screen. Record all observations and
be particularly careful to scan the focal plane for small crystals. Observe the
drops once each day for the first week, then once a week there after until the
drops dry out. Records should indicate whether the drop is clear, contains
precipitate, and or crystals. Some find it helpful to describe the drop contents using magnitude and descriptive terms. Example: 4+ yellow/brown
fine precipitate, 2+ small bipyramid crystals, clear drop, 3+ needle shaped
crystals in 1+ white precipitate. One may also employ a standard numerical
scoring scheme (Clear = 0, Precipitate = 1, Unknown =3, Crystal = 4 or
silver bullets bio
TM
Solutions for Crystal Growth
User Guide
Figure 1.
Typical observations in a
crystallization experiment
Clear Drop
Skin /
Precipitate
Precipitate
other format) or even a color based scoring scheme. Figure 1
(left side of page) shows typical examples of what one might
observe in a crystallization experiment.
Interpreting the Drop
Most of the chemicals in the Silver Bullets Bio solutions are
used more than once, albeit in a unique combination with
other chemicals, creating a unique library of 96 reagents. The
duplication can be used to extrapolate which chemical may
be the crystal’s silver bullet.
For example, the appearance of crystals in drops 3, 5, and 15
might indicate that trans-Cinnamic acid is the silver bullet
since trans-Cinnamic acid is a common chemical to each of
these solutions.
It is generally recommended that one first perform subsequent optimization using a complete Silver Bullets Bio rePrecipitate / agent formulation as it may not be necessary to deconvolute
Phase
and screen individual Silver Bullets Bio chemicals to optimize the condition.
Optimization
When optimizing a crystal produced from a Silver Bullets Bio
solution, one should pursue strategies, methods and techniques typically used for crystal optimization. This would include varying the pH, the sample and crystallization reagent
Microcrystals composition and concentration. One should also consider the
effects of temperature between 4 and 30 degrees Celsius. Seed
from anything crystalline or precipitated. Vary drop ratios.
Further purify or modify the sample, optimize sample bufNeedle
fer and pH. And of course consider evaluating other additives
Cluster
which may be required by your sample to produce or improve
crystals.
HR2-088 (page 2)
Using Silver Bullets Bio with the ThermoFluor Assay
for Protein Stability
The ThermoFluor assay provides a fluorescence readout measurement of thermally-induced protein melting.4,5 A ThermoFluor assay with the protein in the presence of small molecule
additives can be used to help in the identification of sample
buffer formulations that increase Tm and relative stability of
the protein. (Figure 2). 6-8, 10-12
Figure 2. ThermoFluor Principle
The protein in solution is heated in the presence of Silver Bullets
Bio and Sypro Orange. The fluorescence of the hydrophobic dye
Sypro Orange increases significantly when the dye binds to the
internal hydrophobic protein patches that become exposed upon
protein denaturation. Protein stability or melting temperature
(Tm) can be measured by analyzing the temperature dependence
of protein denaturation and subsequent increase in fluorescence
intensity. Silver Bullets Bio that stabilize or destabilize the protein
can be identified by the measured increased or decreased Tm.
Lower Stability
Control Experiment
Plates
A control experiment can be used to identify false leads, or
salt crystals, where all components are present from the
original crystallization experiment, minus the sample. The
Rod Cluster sample solution, complete with all chemicals, minus the
sample should be used in place of the original sample. The
presence of crystals in the control drop that appear similar to
those in the original crystallization experiment indicates the
formation of salt crystals. For best interpretation, the control
Single
Crystal
experiment should be performed using the same crystallization method, with the same device, materials, temperature,
and volumes and evaluated on the same time scale and in
a manner similar to the original crystallization experiment.
LEGEND
Quasi
Crystals
Sypro Orange
Denatured
Protein
Native,
Stable Protein
Silver Bullets Bio
Higher Stability
silver bullets bio
User Guide
Sample Preparation for ThermoFluor
Sample purity should be 75% or greater for reliable ThermoFluor experiments. It is desirable to remove affinity tags from the protein sample before
ThermoFluor. Tags can have high dynamic structure and destabilize the
protein during ThermoFluor or have their own contribution to the melting
curve. A tag can decrease protein thermal stability as well as complicate
ThermoFluor data and interpretation of the results.
ThermoFluor Assay with Silver Bullets Bio
ThermoFluor assay for Silver Bullets Bio with a final well volume of 20 μl.
With the PCR plate on ice, add the following to each well.
1. Pipette 2 μl of 1 mg/mL protein per well (2 μg of protein per well).
2. Pipette 11 μl of deionized water per well.
3. Pipette 5 μl of Silver Bullets Bio per well.
4. Pipette 2 μl of 50x Sypro Orange per well. *Add this last*
(Note: To make 200 μl of 50x Sypro Orange add 2 μl of 5000x Sypro
Orange to 198 μl of deionized water.)
5. Repeat 1-4 for each well of the PCR reaction plate.
6. Seal the PCR reaction plate with transparent sealing film.
7. Centrifuge the PCR reaction plate at 4°C at 2,500 g for 30 seconds to
bring all of the solution to the bottom of the well and to rid each well
of any bubbles.
8. Place the sealed plate in the RT-PCR instrument equilibrated to 4°C
or 25°C, programmed for a 5 minute equilibration in order for the
temperature of the plate and reagents to equilibrate with the block and
as well as allow the reagents to diffuse and mix.
9. Perform the ThermoFluor assay from either 4 to 95°C or 25 to 95°C
with a temperature ramp of 1°C per minute.
* Step 3 is a 1:4 dilution. Other dilutions can and should be assayed to find
the optimal Silver Bullets Bio concentration. Analyze the ThermoFluor data
to identify the optimal Silver Bullets Bio for the sample.9, 13-15
Using Silver Bullets Bio with Dynamic Light Scattering
Dynamic Light Scattering (DLS) measurements can be performed on any
single or multi-angle light scattering instrument. The volume required will
depend upon how the instrument is equipped as well as the type and volume
of cuvette or plate. The minimum protein concentration required for a good
DLS signal will depend upon the molecular weight of the protein. An often
mentioned concentration is 1 mg/ml, however a protein of 15 kD might
perform well at 3 mg/ml, a 100 kD protein at 0.5 mg/ml. Follow the manufacturer’s recommendation for setting up the instrument, temperature options and general experimental protocol. For the DLS assay using the Silver
Bullets Bio, the suggested initial assay concentration is to dilute the Silver
Bullets Bio 1:4. For example, if your assay volume is 4 μl, add 1 μl of reagent
to 3 μl of protein. If the assay volume is 40 μl, add 10 μl of reagent to 30 μl
of protein. If possible, perform measurements at temperatures between 4 37°C as the dispersity profile can vary with temperature. Evaluate the data to
TM
Solutions for Crystal Growth
HR2-088 (page 3)
identify reagents that promote a monidisperse DLS profile. In general, good
sample monodispersity is a radius < 5 nm and polydispersity < 25%.16-18
Silver Bullets Bio Formulation
Silver Bullets Bio reagents are %w/v solution in Type 1+ ultrapure water,
18.2 MΩ•cm resistivity at 25°C, < 5 ppb Total Organic Carbon, < 1 Bacteria
(CFU/ml), < 0.03 Endotoxin (EU/ml) and HEPES sodium pH 6.8 buffer.
Once the chemicals are added to the buffer and water, the solution is warmed
to 50 degrees Celsius to enhance chemical solubility, followed by incubation
at 25 degrees Celsius. The pH of each reagent is adjusted to pH 6.8 at 25
degrees Celsius using Hydrochloric acid or Sodium hydroxide. The resulting solution is centrifuged to pellet any precipitate and the supernatant is
filtered using a 0.22 micron sterile filter into a sterile container. HEPES
sodium buffer CAS [75277-39-3] is titrated to pH 6.8 using Hydrochloric
acid. Refer to the Excel document entitled “Silver Bullets Specifications”
for complete chemical names, synonyms, molecular formula, Mr, CAS, EC,
structure, Beilstein Number and RTECS information. Each Silver Bullets
Bio solution is available separately through the Hampton Research Custom
ShopTM.
Reagent Solubility
Many Silver Bullets Bio are formulated at or near saturation at pH 6.8 in
0.02 M HEPES sodium buffer. The solubility is temperature dependent, pH
dependent and also depends on the presence of other chemicals in the reagent. Storage or exposure of the reagents to temperatures below 25 degrees
Celsius will precipitate some of the Silver Bullets Bio. This is normal. When
this happens, the reagents should be warmed to 50 degrees Celsius for up
to 4 hours and mixed to promote solubility. Allow the Silver Bullets Bio
to re-equilibrate at 25 degrees Celsius and centrifuge the block before use.
Centrifuge the block at 1,000 rpm for 10 minutes to sediment any remaining
precipitate. When setting the crystallization experiment use only the supernatant. Avoid touching the pipette tips to the bottom of the reagent well to
avoid clogging tips or suspending the precipitate.
The chemicals in Silver Bullets Bio are capable of forming small molecule
salt crystals (false positives). Salt crystals may form due to:
• Changes in pH
• A lowering of temperature
• An increase in the relative supersaturation of the reagent
• The presence of polyvalent ions
• The presence of salts (including but not limited to phosphate,
borate and carbonate)
• Evaporation
To minimize evaporation from the open block, place a Plate Lid (HR3-084 /
HR3-085) on top of the opened block when it is not being stored or in active
use. When viewing crystallization experiment drops, one should be aware
of the possibility of salt crystals. To test for the presence of salt crystals, it is
suggested one set a control experiment (see page 2).
silver bullets bio
User Guide
Silver Bullets Bio & pH
Silver Bullets Bio reagents are buffered in 0.02 M HEPES sodium pH 6.8
but can be screened at different pH levels in the presence of higher buffer
concentrations, such as 0.1 M.
Storing, Handling & Use
Silver Bullets Bio should be stored between -20 and 25 degrees Celsius. If
the solutions have been below 25 degrees Celsius, or if precipitate is present,
warm the reagents to 50 degrees Celsius for up to 4 hours, mix well and then
allow the solutions to equilibrate to 25 degrees Celsius. Centrifuge the 96
well plate that contains the Silver Bullets Bio at 1,000 RPM for 10 minutes to
pellet any remaining precipitate. The solutions are now ready for use.
The Deep Well block is thermal sealed with a polypropylene/aluminum film.
The film should be removed when the block is at a temperature of 25 degrees
Celsius. Removing the film with the block temperature below 25 degrees
Celsius can delaminate or shred the film. After use, seal the block using
AlumaSeal II Sealing Film (HR8-069), or the block could be sealed using a
thermal sealer.
References
1. Searching for silver bullets: An alternative strategy for crystallizing
macromolecules. Alexander McPherson and Bob Cudney. Journal of
Structural Biology 156 (2006) 387-406.
2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney, and Alexander
McPherson. Acta Cryst. (2007) D63, 310-318.
3. A new crystal form of bovine pancreatic RNase A in complex with 2’-deoxyguanosine-5’-monophosphate. S. B. Larson, J. S. Day, R. Cudney
and A. McPherson. Acta Cryst. (2007). F63, 728-733.
4. High-Density Miniaturized Thermal Shift Assays as a General Strategy
for Drug Discovery. Pantoliano et al, Journal of Biomolecular Screening, Volume 6, Number 6, 2001.
5. Beta-Sheet.org web site. Retrieved February 17, 2014 from http://www.
beta-sheet.org/page11/index.html.
6. A thermal stability assay can help to estimate the crystallization likelihood of biological samples. Marquez et al. Acta Cryst. (2011). D67,
915-919.
7. The combined use of the ThermoFluor assay and ThermoQ analytical
software for the determination of protein stability and buffer optimization as an aid in protein crystallization. Kevin Phillips and Andres
Hernandez de la Pena. Current Protocols in Molecular Biology 10.28.110.28.15 April 2011.
8. Thermofluor-based optimization strategy for the stabilization and
crystallization of Campylobacter jejuni desulforubrerythrin. Romao et
al. Protein Expression and Purification 81 (2012) 193-200.
9. High-Density Miniaturized Thermal Shift Assays as a General Strategy
for Drug Discovery. Pantoliano et al, Journal of Biomolecular Screening, Volume 6, Number 6, 2001.
TM
Solutions for Crystal Growth
HR2-088 (page 4)
10. ThermoFluor-based high-throughput stability optimization of proteins for structural studies. Ericsson, U.B. et al, Anal Biochem. 2006 Oct
15;357(2):289-98. Epub 2006 Aug 10.
11. Chemical screening methods to identify ligands that promote protein
stability, protein crystallization, and structure determination. Vedadi,
M. et al, Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15835-40. Epub
2006 Oct 11.
12. Comparison of fluorescence and light scattering based methods to
assess formation and stability of protein–protein complexes. J. Kopec,
G. Schneider. Journal of Structural Biology 175 (2011) 216–223.
13. Enhancing recombinant protein quality and yield by protein stability profiling. Mezzasalma TM, Kranz JK, Chan W, Struble GT, SchalkHihi C, Deckman IC, Springer BA, Todd MJ. J Biomol Screen. 2007
Apr;12(3):418-28.
14. ThermoFluor-based optimization strategy for the stabilization and
crystallization of Campylobacter jejuni desulforubrerythrin. Santos SP,
Bandeiras TM, Pinto AF, Teixeira M, Carrondo MA, Romão CV. Protein
Expr Purif. 2012 Feb;81(2):193-200. doi: 10.1016/j.pep.2011.10.001.
Epub 2011 Oct 24.
15. Optimization of protein purification and characterization using
ThermoFluor screens. Boivin S, Kozak S, Meijers R. Protein Expr Purif.
2013 Oct;91(2):192-206. doi: 10.1016/j.pep.2013.08.002. Epub 2013
Aug 12.
16. Use of dynamic light scattering to assess crystallizability of macromolecules and macromolecular assemblies. Ferré-D’Amaré AR1, Burley SK. Structure. 1994 May 15;2(5):357-9.
17. Crystallizing proteins - a rational approach? D’Arcy A. Acta Crystallogr
D Biol Crystallogr. 1994 Jul 1;50(Pt 4):469-71.
18. Protein Crystallization. Techniques, Strategies, and Tips. A Laboratory
Manual. Edited by Terese M. Bergfors. International University Line.
1999; 27-38.
Technical Support
Inquiries regarding Silver Bullets Bio solution formulation, interpretation
of screen results, optimization strategies and general inquiries regarding
crystallization are welcome at [email protected].
ThermoFluor® is a registered trademark of Johnson & Johnson.
Sypro® is a registered trademark of Thermo Fisher Scientific Inc.
silver bullets bio
TM
Also in
Well #
Wells A1 - A12
[units]
Silver Bullet Bio
CAS
EC
CAS
EC
1, 54, 62
0.16% w/v
L-Citrulline
175.19
372-75-8
2067596
7, 11, 91
0.16% w/v
Benzamidine hydrochloride
156.61
1670-14-0
2167954
1, 88
0.16% w/v
L-Ornithine hydrochloride
168.62
3184-13-2
2216786
7, 8, 19
0.16% w/v
Acarbose
645.61
56180-94-0
2600307
1
0.16% w/v
Urea
60.06
57-13-6
2003155
7
0.16% w/v
Ethidium bromide
394.31
1239-45-8
2149846
0.16% w/v
Oxalic acid
90.04
144-62-7
2056343
7, 81
0.16% w/v
Cholesterol
386.66
57-88-5
2003532
0.16% w/v
Kanamycin monosulfate
582.58
25389-94-0
2469339
7, 19
0.16% w/v
Sarcosine
89.09
107-97-1
2035386
1, 10, 21, 42, 54, 85
0.16% w/v
L-Arginine
174.20
74-79-3
2008111
7, 48
0.16% w/v
L-Homoserine
119.12
672-15-1
2115906
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2, 12, 95
0.16% w/v
L-Carnitine hydrochloride
197.66
6645-46-1
2296636
8, 51
0.20% w/v
Sodium gluconate
218.14
527-07-1
2084077
2
0.16% w/v
Tannic acid
1701.22
1401-55-4
2157532
7, 8, 19
0.20% w/v
Acarbose
645.61
56180-94-0
2600307
2, 18, 92
0.16% w/v
Aspartame
294.30
22839-47-0
2452613
8, 96
0.20% w/v
D-(+)-Maltose monohydrate
360.32
6363-53-7
2007165
0.16% w/v
Caffeine
194.19
58-08-2
2003621
0.20% w/v
Salicylic acid
138.12
69-72-7
2007123
0.16% w/v
p-Coumaric acid
164.16
501-98-4
2, 48
0.16% w/v
4-Hydroxy-L-proline
131.13
51-35-4
2000919
D-(+)-Melibiose monohydrate
342.30
585-99-9
2095686
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2781697
1
1, 10
1, 83
(A1)
2
2, 17, 92
2, 16
(A2)
MW
Also in
3, 64
0.16% w/v
Disodium beta-Glycerophosphate tetrahydrate
216.04
819-83-0
2124643
3, 5, 15
0.16% w/v
trans-Cinnamic acid
148.16
140-10-3
2053981
0.16% w/v
O-Phospho-L-tyrosine
261.17
21820-51-9
0.16% w/v
Betaine anhydrous
117.15
107-43-7
2034906
0.16% w/v
Maltotriose hydrate
504.44
207511-08-8
2141742
3, 48
0.16% w/v
Cytidine
243.22
65-46-3
2006109
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
3, 17, 94
3, 21
3, 83
3
(A3)
(anhyd)
4, 14
0.16% w/v
L-Canavanine
176.18
543-38-4
4, 13, 96
0.16% w/v
O-Phospho-L-serine
185.07
407-41-0
4
Taurine
125.15
107-35-7
2034838
0.16% w/v
Quinine
324.42
130-95-0
2050032
0.16% w/v
Sodium glyoxylate monohydrate
114.03
2706-75-4
2202988
4, 49
0.16% w/v
Cholic acid
408.58
81-25-4
2013378
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
5, 20
0.08% w/v
Ellipticine
246.31
519-23-3
2082640
5, 17
0.20% w/v
Gibberellin A 3
346.38
77-06-5
2010010
4, 83
(A4)
5
0.20% w/v
trans-Cinnamic acid
148.16
140-10-3
2053981
(A5)
0.20% w/v
Phenol
94.11
108-95-2
2036327
3, 5, 15
5
5, 82
0.20% w/v
Buffer
0.02 M
6, 12
0.16% w/v
6, 21
0.16% w/v
6, 67
6
6, 20
6, 82
(A6)
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Sulfanilamide
172.20
63-74-1
2005634
D-(+)-Cellobiose
342.30
528-50-7
143.12
14028-62-7
0.16% w/v
6-Phosphogluconic acid trisodium salt
342.08
53411-70-4
0.16% w/v
Buffer
0.02 M
Na-Benzoyl-L-arginine ethyl ester hydrochloride
342.83
2645-08-1
(A7)
8
(A8)
[units]
0.20% w/v
Silver Bullet Bio
MW
(anhyd)
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
9, 11
0.20% w/v
g-Strophanthin
728.78
630-60-4
9, 14, 96
9
9, 20
0.20% w/v
D-Sorbitol
182.17
50-70-4
2000615
9
0.20% w/v
1,4-Diaminobutane
88.15
110-60-1
2037823
(A9)
0.20% w/v
D-(+)-Trehalose dihydrate
378.33
6138-23-4
2027396
L-a-Phosphatidylcholine
9, 80
0.20% w/v
Buffer
0.02 M
8002-43-5
2323072
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
10
10, 18
0.16% w/v
Sucrose
342.30
57-50-1
2003349
0.16% w/v
Cadaverine
102.18
462-94-2
2073290
0.16% w/v
L-Glutamic acid
147.13
56-86-0
2002937
0.16% w/v
L-Arginine
174.20
74-79-3
2008111
1, 10
0.16% w/v
Oxalic acid
90.04
144-62-7
2056343
10, 79
0.16% w/v
Tetrahydrofolic acid
445.43
135-16-0
2051811
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
11, 20
0.20% w/v
Protamine sulfate salt
9, 11
0.20% w/v
g-Strophanthin
728.78
630-60-4
10, 42, 54, 85
1, 10, 21, 42,
54, 85
2084365
10
(A10)
53597-25-4
7, 11, 91
11
0.20% w/v
Benzamidine hydrochloride
156.61
1670-14-0
2167954
11, 68, 90
(A11)
0.20% w/v
D-Fructose 1,6-diphosphate trisodium salt octahydrate
550.18
81028-91-3
2537780
11, 81
0.20% w/v
Oxamic acid
89.05
471-47-6
2074430
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
12, 26, 95
0.20% w/v
2’-Deoxyadenosine 5’-triphosphate disodium salt
535.15
1927-31-7
2176623
12, 96
0.20% w/v
Benzidine
184.24
92-87-5
2021991
12
0.20% w/v
L-Carnitine hydrochloride
197.66
6645-46-1
2296636
(A12)
2, 12, 95
2585436
6, 12
0.20% w/v
Sulfanilamide
172.20
63-74-1
2005634
2201570
12, 28, 37, 38, 91
0.20% w/v
Cytosine
111.10
71-30-7
2007495
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
D-(+)-Galactosamine hydrochloride
215.63
1772-03-8
2171981
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Formulation Sheet (HR2-088)
7
8, 16
6106-21-4
D-Glyceric acid calcium salt dihydrate
0.16% w/v
-
270.14
0.16% w/v
6, 51
Silver Bullets Bio
Succinic acid disodium salt hexahydrate
8, 14
2069860
0.16% w/v
4
4, 16
Well #
Page 1 of 8
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
Well #
[units]
Silver Bullet Bio
Wells B1 - B12
MW
CAS
EC
Also in
CAS
EC
19, 35, 90
Well #
0.16% w/v
[units]
Silver Bullet Bio
Thymidine
242.23
MW
50-89-5
2000704
0.16% w/v
Adenosine 3’,5’-cyclic monophosphate sodium salt
monohydrate
369.20
37839-81-9
2533283
0.16% w/v
Sarcosine
89.09
107-97-1
2035386
13, 25, 81, 91
0.20% w/v
2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate
347.22
13, 61
0.20% w/v
N-Acetyl-D-galactosamine
221.21
1811-31-0
2173219
18, 19, 63
0.20% w/v
O-Phospho-L-serine
185.07
407-41-0
2069860
7, 19
0.20% w/v
4-Aminobenzoic acid
137.14
150-13-0
2057530
13, 19, 91
0.16% w/v
4-Aminobenzoic acid
137.14
150-13-0
2057530
13, 36, 89
0.20% w/v
Uridine
244.20
58-96-8
2004075
7, 8, 19
0.16% w/v
Acarbose
645.61
56180-94-0
2600307
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
19, 48
0.16% w/v
Inosine
268.23
58-63-9
2003904
14, 24, 91
0.16% w/v
Thymidine 5’-triphosphate sodium salt
504.15
18423-43-3
4, 14
0.16% w/v
L-Canavanine
176.18
543-38-4
9, 14, 96
0.16% w/v
D-Sorbitol
182.17
50-70-4
0.16% w/v
Salicylic acid
138.12
69-72-7
2'-Deoxyguanosine hydrate
267.24
961-07-9
4, 13, 96
13, 19, 91
8, 14
14, 34, 73
13
(B1)
14
(B2)
0.16% w/v
14, 80
0.16% w/v
Buffer
0.02 M
15, 94
0.16% w/v
15, 64
15
0.16% w/v
(anhyd)
(anhyd)
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
5, 20
0.08% w/v
Ellipticine
246.31
519-23-3
2082640
2000615
11, 20
0.20% w/v
Protamine sulfate salt
2007123
9, 20
20
0.20% w/v
D-(+)-Trehalose dihydrate
378.33
6138-23-4
2027396
(B8)
0.20% w/v
6-Phosphogluconic acid trisodium salt
342.08
53411-70-4
2585436
0.20% w/v
D-(+)-Glucose
180.16
50-99-7
2000751
6, 20
D-(+)-Raffinose pentahydrate
594.52
17629-30-0
2081469
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2528493
21
0.20% w/v
Penicillin G sodium salt
356.38
69-57-8
2007102
2074273
1, 10, 21, 42,
54, 85
0.20% w/v
L-Arginine
174.20
74-79-3
2008111
0.20% w/v
D-(+)-Cellobiose
342.30
528-50-7
2084365
0.20% w/v
Pyridoxamine dihydrochloride
241.11
524-36-7
2083576
Betaine anhydrous
117.15
107-43-7
2034906
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Cytidine 5’-triphosphate disodium salt
Stachyose hydrate
527.12
666.58
36051-68-0
54261-98-2
Cystathionine
222.26
535-34-2
2086137
Cystamine dihydrochloride
225.20
56-17-7
2002607
0.16% w/v
trans-Cinnamic acid
148.16
140-10-3
2053981
15, 17, 62
0.16% w/v
Inosine 5’-monophosphate disodium salt
392.17
352195-40-5
2251464
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
16, 27
0.20% w/v
Uridine 5’-diphosphate sodium salt
404.16
21931-53-3
4, 16
0.20% w/v
Quinine
324.42
130-95-0
(B3)
16
0.20% w/v
p-Coumaric acid
164.16
501-98-4
8, 16
(B4)
0.20% w/v
D-(+)-Melibiose monohydrate
342.30
585-99-9
2095686
15, 16, 92
0.20% w/v
Cystamine dihydrochloride
225.20
56-17-7
2002607
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
17, 22
0.20% w/v
Nicotinic acid
123.11
59-67-6
2004410
0.20% w/v
Inosine 5’-monophosphate disodium salt
392.17
352195-40-5
2251464
(anhyd)
6, 21
21, 77, 94
21
(B9)
3, 21
0.20% w/v
Buffer
0.02 M
17, 22
0.20% w/v
Nicotinic acid
123.11
59-67-6
2004410
0.20% w/v
Adenosine
267.24
58-61-7
2003899
0.20% w/v
L-Tyrosine
181.19
60-18-4
2004604
0.20% w/v
Xanthine
152.11
69-89-6
2007186
22, 41
0.20% w/v
L-Lactic acid
90.08
79-33-4
2011962
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
23, 32
0.20% w/v
2’-Deoxyadenosine monohydrate
269.26
16373-93-6
2134887
0.20% w/v
N-Acetylneuraminic acid
309.27
131-48-6
2050231
0.20% w/v
N-p-Tosyl-L-phenylalanine chloromethyl ketone
351.85
402-71-1
2069546
0.20% w/v
a-D-Glucose 1-phosphate disodium salt hydrate
304.10
56401-20-8
2601541
23, 78
0.20% w/v
Fumaric acid
116.07
110-17-8
2037430
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
22, 31, 88
2050032
2, 16
15, 17, 62
53597-25-4
20, 79
0.16% w/v
3 ,5, 15
(B7)
Buffer
0.16% w/v
15
15, 16, 92
19
22, 33, 44, 54, 66,
85, 93
22, 35
22
(B10)
23, 36, 56, 93
(anhyd)
17
0.20% w/v
Gibberellin A 3
346.38
77-06-5
(B5)
0.20% w/v
O-Phospho-L-tyrosine
261.17
21820-51-9
2, 17, 92
0.20% w/v
Caffeine
194.19
58-08-2
2003621
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
18, 19, 63
0.20% w/v
Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate
369.20
37839-81-9
2533283
14, 24, 91
0.25% w/v
Thymidine 5’-triphosphate sodium salt
504.15
18423-43-3
10, 18
0.20% w/v
Cadaverine
102.18
462-94-2
2073290
24, 34
0.25% w/v
a-Ketoglutaric acid disodium salt
190.06
305-72-6
2061678
0.20% w/v
D-(+)-Melezitose hydrate
504.44
207511-10-2
2098949
24, 36
0.25% w/v
2-Nitrophenyl b-D-galactopyranoside
301.25
369-07-3
2067161
0.25% w/v
cis-Aconitic acid
174.11
585-84-2
2095644
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
5, 17
3, 17, 94
18, 65
2, 18, 92
18
(B6)
0.20% w/v
18, 33, 90
0.20% w/v
Buffer
0.02 M
Silver Bullets Bio
-
Aspartame
(anhyd)
294.30
22839-47-0
2010010
23, 38, 95
2452613
24, 78
Buffer
Guanosine
283.24
118-00-3
2042278
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Formulation Sheet (HR2-088)
23
Page 2 of 8
23
(B11)
24
(B12)
0.02 M
(anhyd)
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
Well #
[units]
Silver Bullet Bio
Wells C1 - C12
MW
CAS
Also in
EC
0.20% w/v
2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate
0.20% w/v
Ethanolamine
61.08
141-43-5
2054833
0.20% w/v
Theophylline
180.17
58-55-9
2003857
0.20% w/v
Isopropyl 1-thio-b-D-galactopyranoside
238.30
367-93-1
2067030
25, 78
0.20% w/v
Oxalacetic acid
132.07
328-42-7
2063298
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
12, 26, 95
0.20% w/v
2’-Deoxyadenosine 5’-triphosphate disodium salt
535.15
1927-31-7
2176623
26, 33, 55, 94
0.20% w/v
D-Glucose 6-phosphate sodium salt
282.12
54010-71-8
2589210
0.20% w/v
Acetylsalicylic acid
180.16
50-78-2
2000641
0.20% w/v
Choline base solution
121.18
123-41-1
26, 76
0.20% w/v
Nicotinamide
122.12
98-92-0
2027134
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
16, 27
0.20% w/v
Uridine 5’-diphosphate sodium salt
404.16
21931-53-3
27, 37
0.20% w/v
L-Carnosine
226.24
305-84-0
2061699
13, 25, 81, 91
25, 39, 40
25
25, 39
25, 38
(C1)
26
26, 40, 49, 88
26, 39, 95
(C2)
Well #
22, 31, 88
347.22
(anhyd)
28, 31
CAS
EC
0.20% w/v
[units]
Silver Bullet Bio
Adenosine
267.24
MW
58-61-7
2003899
0.20% w/v
Pepstatin A
685.91
26305-03-3
2476000
31
0.20% w/v
(±)-Epinephrine
183.21
329-65-7
2063476
(C7)
0.20% w/v
Sodium phenyl phosphate dibasic dihydrate
254.09
66778-08-3
2219174
31, 72
0.20% w/v
Inosine 5’-triphosphate trisodium salt
574.11
35908-31-7
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
23, 32
0.20% w/v
2’-Deoxyadenosine monohydrate
269.26
16373-93-6
2134887
30, 31, 69
27, 31, 96
29, 32
0.20% w/v
O-Phospho-DL-threonine
199.10
27530-80-9
2485125
32
0.20% w/v
Flavin adenine dinucleotide disodium salt hydrate
829.51
84366-81-4
2827338
(C8)
0.20% w/v
DL-Xylose
150.13
25990-60-7
2473958
32, 71
0.20% w/v
Nalidixic acid
232.24
389-08-2
2068647
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
32, 66
32
18, 33, 90
0.20% w/v
Guanosine
283.24
118-00-3
2042278
26, 33, 55, 94
0.20% w/v
D-Glucose 6-phosphate sodium salt
282.12
54010-71-8
2589210
33, 40
33
0.20% w/v
2,3-Pyridinedicarboxylic acid
167.12
89-00-9
2018748
(C9)
0.20% w/v
L-Tyrosine
181.19
60-18-4
2004604
Adenosine 5’-diphosphate sodium salt
427.20
20398-34-9
HEPES sodium pH 6.8
260.29
75277-39-3
27, 41, 93
27
0.20% w/v
L-Thyroxine
776.87
51-48-9
2001011
27, 31, 96
(C3)
0.20% w/v
Sodium phenyl phosphate dibasic dihydrate
254.09
66778-08-3
2219174
22, 33, 44, 54, 66,
85, 93
27, 76
0.20% w/v
Glutaric acid
132.12
110-94-1
2038172
33, 70
0.20% w/v
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Buffer
0.02 M
12, 28, 37, 38, 91
0.20% w/v
Cytosine
111.10
71-30-7
2007495
0.20% w/v
Vitamin B12
1355.37
68-19-9
2006800
14, 34, 73
0.25% w/v
2'-Deoxyguanosine hydrate
267.24
28, 40
961-07-9
0.20% w/v
Pepstatin A
685.91
26305-03-3
2476000
0.25% w/v
Cortisone
360.45
53-06-5
2001624
0.25% w/v
a-Ketoglutaric acid disodium salt
190.06
305-72-6
2061678
0.25% w/v
Pyruvic acid
88.06
127-17-3
2048243
28
28, 31
28, 35
0.20% w/v
(C4)
28, 76
0.20% w/v
Buffer
0.02 M
29
0.20% w/v
29, 36
29, 37
29, 32
29
(C5)
29, 43, 54, 85
Buffer
Shikimic acid
174.15
138-59-0
2053342
N-Acetyl-L-glutamic acid
189.17
1188-37-0
2147084
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2’-Deoxycytidine 5’-triphosphate disodium salt
511.12
102783-51-7
0.20% w/v
Hydrocortisone
362.47
50-23-7
2000201
0.20% w/v
Hemin
651.94
16009-13-5
2401401
0.20% w/v
O-Phospho-DL-threonine
199.10
27530-80-9
2485125
0.20% w/v
L-Phenylalanine
165.19
63-91-2
2005681
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
30, 34
0.04% w/v
Cortisone
360.45
53-06-5
2001624
30, 31, 69
0.04% w/v
(±)-Epinephrine
183.21
329-65-7
2063476
30, 50
30, 37, 41
0.04% w/v
30
0.04% w/v
(C6)
30, 72
0.04% w/v
Buffer
Silver Bullets Bio
0.02 M
-
Protoporphyrin disodium salt
606.62
Pyridoxine
169.18
Thymidine 5’-monophosphate disodium salt hydrate
366.18
HEPES sodium pH 6.8
Formulation Sheet (HR2-088)
(anhyd)
260.29
50865-01-5
65-23-6
33430-62-5
75277-39-3
2568159
30, 34
24, 34
34, 69
34
(C10)
(anhyd)
2781697
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
19, 35, 90
0.20% w/v
Thymidine
242.23
50-89-5
2000704
35, 38
0.20% w/v
trans-Dehydroandrosterone
288.43
53-43-0
2001755
35
0.20% w/v
Xanthine
152.11
69-89-6
2007186
(C11)
0.20% w/v
Shikimic acid
174.15
138-59-0
2053342
35, 68
0.20% w/v
Histamine dihydrochloride
184.07
56-92-8
2002984
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
13, 36, 89
0.20% w/v
Uridine
244.20
58-96-8
2004075
0.20% w/v
Hydrocortisone
362.47
50-23-7
2000201
131-48-6
2050231
22, 35
28, 35
29, 36
23, 36, 56, 93
36
0.20% w/v
N-Acetylneuraminic acid
309.27
(C12)
(anhyd)
2006030
24, 36
0.20% w/v
2-Nitrophenyl b-D-galactopyranoside
301.25
369-07-3
2067161
2515180
36, 65
0.20% w/v
Phospho(enol)pyruvic acid monosodium salt hydrate
208.04
53823-68-0
2588107
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2781697
Page 3 of 8
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
Well #
Wells D1 - D12
[units]
Silver Bullet Bio
MW
CAS
EC
[units]
Silver Bullet Bio
MW
CAS
EC
12, 28, 37, 38, 91
0.20% w/v
Cytosine
111.10
71-30-7
2007495
43, 54, 85
0.125% w/v
L-Methionine
149.21
63-68-3
2005629
30, 37, 41
0.20% w/v
Pyridoxine
169.18
65-23-6
2006030
29, 43, 54, 85
0.125% w/v
L-Phenylalanine
165.19
63-91-2
2005681
2401401
43, 54, 85
0.125% w/v
L-Leucine
131.18
61-90-5
2005220
2061699
43, 54, 85
0.125% w/v
L-Isoleucine
131.18
73-32-5
2007982
0.125% w/v
L-Valine
117.15
72-18-4
2007736
0.125% w/v
L-Alanine
89.09
56-41-7
2002738
43, 54, 72, 85
0.125% w/v
L-Tryptophan
204.23
73-22-3
2007956
43, 54, 85
0.125% w/v
L-Proline
115.13
147-85-3
2057022
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
37
29, 37
27, 37
0.20% w/v
Hemin
651.94
16009-13-5
0.20% w/v
L-Carnosine
226.24
305-84-0
37, 64
0.20% w/v
Cytidine 5’-diphosphocholine sodium salt dihydrate
546.34
33818-15-4
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
(D1)
12, 28, 37, 38, 91
0.20% w/v
Cytosine
111.10
0.20% w/v
a-D-Glucose 1-phosphate disodium salt hydrate
304.10
0.20% w/v
trans-Dehydroandrosterone
0.20% w/v
38, 63
0.20% w/v
Buffer
0.02 M
39, 42
39, 51
23, 38, 95
38
35, 38
25, 38
(D2)
39
26, 39, 95
25, 39
25, 39, 40
2007495
(D7)
2001755
44, 54, 85
0.14% w/v
Glycine
75.07
56-40-6
2002722
Isopropyl 1-thio-b-D-galactopyranoside
238.30
367-93-1
2067030
44, 54, 85
0.14% w/v
L-Asparagine monohydrate
150.13
5794-13-8
2007359
D-(−)-Ribose
150.13
50-69-1
2000594
44, 54, 85
0.14% w/v
L-Glutamine
146.15
56-85-9
2002921
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
22, 33, 44, 54, 66, 85, 93
44
0.14% w/v
L-Tyrosine
181.19
60-18-4
2004604
0.20% w/v
Thiamine pyrophosphate
460.77
154-87-0
2058361
44, 54, 85
(D8)
0.14% w/v
L-Serine
105.09
56-45-1
2002743
0.20% w/v
D-Glucosamic acid
195.17
3646-68-2
2228791
44, 54, 85
0.14% w/v
L-(-)-Threonine
119.12
72-19-5
2007741
0.20% w/v
Choline base solution
121.18
123-41-1
44, 77, 84
0.14% w/v
Maleic acid
116.07
110-16-7
2037425
2003857
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2054833
45
0.14% w/v
Sodium bromide
102.89
7647-15-6
2315999
45
0.14% w/v
Sodium fluoride
41.99
7681-49-4
2316678
45
0.14% w/v
Sodium carbonate
105.99
497-19-8
2078388
45
0.14% w/v
Sodium tetraborate decahydrate
381.37
1303-96-4
2155404
(D9)
0.14% w/v
Sodium phosphate dibasic dihydrate
177.99
10028-24-7
2314487
45
0.14% w/v
Sodium nitrate
84.99
7631-99-4
2315543
45
0.14% w/v
Rhenium(IV) oxide
218.21
12036-09-8
2348390
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2331408
0.20% w/v
33, 40
43, 54, 85
53-43-0
0.20% w/v
28, 40
43, 54, 85
2781697
288.43
0.02 M
26, 40, 49, 88
43
2601541
0.20% w/v
Buffer
Well #
56401-20-8
0.20% w/v
(D3)
71-30-7
Also in
Theophylline
Ethanolamine
HEPES sodium pH 6.8
Acetylsalicylic acid
Vitamin B12
(anhyd)
180.17
61.08
260.29
180.16
1355.37
58-55-9
141-43-5
75277-39-3
50-78-2
68-19-9
2781697
2000641
2006800
45
45
0.20% w/v
2,3-Pyridinedicarboxylic acid
167.12
89-00-9
2018748
0.20% w/v
Ethanolamine
61.08
141-43-5
2054833
40
0.20% w/v
Thiamine hydrochloride
337.27
67-03-8
2006418
Buffer
0.02 M
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
46
0.002 M
Calcium chloride dihydrate
147.01
10035-04-8
0.002 M
Cadmium chloride hydrate
183.32
654054-66-7
46
0.002 M
Magnesium chloride hexahydrate
203.30
7791-18-6
2320946
(D10)
0.002 M
Manganese(II) chloride tetrahydrate
197.91
13446-34-9
2318696
0.002 M
Zinc chloride
136.30
7646-85-7
2315920
25, 39, 40
40
(D4)
(anhyd)
22, 41
0.16% w/v
L-Lactic acid
90.08
79-33-4
2011962
46
41, 42, 54, 85
0.16% w/v
L-Aspartic acid
133.10
56-84-8
2002916
46
2001011
46
27, 41, 93
30, 37, 41
41, 79
0.16% w/v
41
(D5)
L-Thyroxine
776.87
51-48-9
0.16% w/v
Pyridoxine
169.18
65-23-6
2006030
0.16% w/v
L-Ascorbic acid
176.12
50-81-7
2000662
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
47
0.002 M
Nickel(II) chloride hexahydrate
237.69
7791-20-0
2317430
47
0.002 M
Copper(II) chloride dihydrate
170.48
10125-13-0
2312102
0.002 M
Cobalt(II) chloride hexahydrate
237.93
7791-13-1
2315894
0.002 M
Molybdenum(III) chloride
202.29
13478-18-7
2367759
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Phytic acid sodium salt hydrate
660.04
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
47
0.16% w/v
Thiamine pyrophosphate
460.77
154-87-0
2058361
47
41, 42, 54, 85
0.16% w/v
L-Aspartic acid
133.10
56-84-8
2002916
Buffer
0.02 M
10, 42, 54, 85
0.16% w/v
L-Glutamic acid
147.13
56-86-0
2002937
7, 48
0.16% w/v
L-Homoserine
119.12
672-15-1
2115906
2002942
2, 48
0.16% w/v
4-Hydroxy-L-proline
131.13
51-35-4
2000919
334.24
918149-29-8
41, 63
0.16% w/v
Buffer
0.02 M
39, 42
42
42, 54, 85
1, 10, 21, 42,
54, 85
0.16% w/v
(D6)
0.16% w/v
42, 54, 85
0.16% w/v
Buffer
Silver Bullets Bio
0.02 M
-
L-(+)-Lysine
L-Arginine
L-Histidine
HEPES sodium pH 6.8
Formulation Sheet (HR2-088)
(anhyd)
146.19
174.20
155.16
260.29
14306-25-3
46
(anhyd)
56-87-1
74-79-3
71-00-1
75277-39-3
2382426
2008111
2007453
2781697
Page 4 of 8
47
(D11)
0.16% w/v
Argininosuccinic acid disodium salt hydrate
(anhyd)
(anhyd)
0.16% w/v
Cytidine
243.22
65-46-3
2006109
0.16% w/v
Inosine
268.23
58-63-9
2003904
48, 53
0.16% w/v
Guanine
151.13
73-40-5
2007998
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
48
3, 48
19, 48
48
(D12)
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
Well #
49, 82, 90
49
49
Silver Bullet Bio
MW
CAS
Thiamine monophosphate
chloride dihydrate
416.82
0.16% w/v
Acetylsalicylic acid
180.16
50-78-2
0.16% w/v
Cholic acid
408.58
81-25-4
0.16% w/v
26, 40,
49, 88
4, 49
[units]
273724-21-3
0.16% w/v
1,2,3-Heptanetriol
148.20
103404-57-5
49, 53
0.16% w/v
Vanillin
152.15
121-33-5
49, 56
0.16% w/v
N-Acetyl-D-mannosamine
221.21
7772-94-3
(E1)
Buffer
0.02 M
50
30, 50
EC
Wells E1 - E12
[units]
Silver Bullet Bio
CAS
EC
1, 54, 62
0.05% w/v
L-Citrulline
175.19
372-75-8
2067596
44, 54, 85
0.05% w/v
Glycine
75.07
56-40-6
2002722
2000641
44, 54, 85
0.05% w/v
L-(-)-Threonine
119.12
72-19-5
2007741
2013378
42, 54, 85
0.05% w/v
L-(+)-Lysine
146.19
56-87-1
2002942
43, 54, 85
0.05% w/v
L-Alanine
89.09
56-41-7
2002738
2085369
2044652
Also in
Well #
1, 10, 21, 42,
54, 85
MW
0.05% w/v
L-Arginine
174.20
74-79-3
2008111
44, 54, 85
0.05% w/v
L-Asparagine
monohydrate
150.13
5794-13-8
2007359
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
0.20% w/v
Adenosine 5’-(b,g-imido)
triphosphate tetralithium salt
hydrate
529.93
72957-42-7
2771275
41, 42,
54, 85
0.05% w/v
L-Aspartic acid
133.10
56-84-8
2002916
0.20% w/v
Protoporphyrin disodium salt
606.62
50865-01-5
2568159
10, 42,
54, 85
0.05% w/v
L-Glutamic acid
147.13
56-86-0
2002937
0.20% w/v
Coproporphyrin I
dihydrochloride
727.64
69477-27-6
44, 54, 85
0.05% w/v
L-Glutamine
146.15
56-85-9
2002921
50, 56
0.20% w/v
Tris
121.14
77-86-1
2010644
50, 55
0.20% w/v
DL-Glyceraldehyde
90.08
56-82-6
2002900
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
39, 51
0.16% w/v
D-Glucosamic acid
195.17
3646-68-2
2228791
6, 51
0.16% w/v
D-(+)-Galactosamine
hydrochloride
215.63
1772-03-8
2171981
D-(+)-Glucosamine
hydrochloride
215.63
50, 53
51
8, 51
50
(E2)
51
(E3)
0.16% w/v
0.16% w/v
Sodium gluconate
218.14
66-84-2
527-07-1
0.16% w/v
N-Acetyl-Dglucosamine
221.21
7512-17-6
2313682
50, 56
0.16% w/v
Tris
121.14
77-86-1
2010644
55, 56
0.16% w/v
L-(+)-Arabinose
150.13
5328-37-0
2262146
Buffer
0.02 M
HEPES sodium
pH 6.8
260.29
75277-39-3
2781697
57, 58
0.16% w/v
Tyr-Phe
328.37
17355-11-2
57, 59
0.16% w/v
Leu-Leu
244.34
3303-31-9
2219766
57, 60, 86
0.16% w/v
Ala-Leu
202.25
3303-34-2
2219771
0.16% w/v
Val-Ser
204.23
13588-94-8
0.16% w/v
Trp-Gly hydrochloride
297.74
63193-13-5
0.16% w/v
Met-Gly
206.26
14486-03-4
2384879
HEPES sodium
pH 6.8
260.29
75277-39-3
2781697
56, 84, 89
61-90-5
2005220
57
43, 54, 85
0.05% w/v
L-Methionine
149.21
63-68-3
2005629
29, 43,
54, 85
0.05% w/v
L-Phenylalanine
165.19
63-91-2
2005681
43, 54, 85
0.05% w/v
L-Proline
115.13
147-85-3
2057022
2006381
44, 54, 85
0.05% w/v
L-Serine
105.09
56-45-1
2002743
2084077
43, 54,
72, 85
260.29
75277-39-3
52, 81
0.16% w/v
D-Glucosamine 6-phosphate
sodium salt
259.15
70442-23-8
1.00% w/v
Tryptone
0.16% w/v
Isomaltose
49, 53
7772-94-3
131.18
HEPES sodium pH 6.8
0.05% w/v
L-Tryptophan
204.23
73-22-3
2007956
0.16% w/v
Tyr-Phe
328.37
17355-11-2
58, 60
0.16% w/v
Tyr-Leu
294.35
17355-10-1
58, 86
0.16% w/v
Z-Val-Phe
398.46
19542-51-9
0.16% w/v
Gly-Gly-Gly
189.17
556-33-2
2091220
2177517
58, 59
1948-31-8
297.74
63193-13-5
Buffer
0.02 M
HEPES sodium
pH 6.8
260.29
75277-39-3
2781697
59, 60, 86
0.20% w/v
Leu-Gly-Gly
245.28
1187-50-4
2146981
2262146
57, 59
0.20% w/v
Leu-Leu
244.34
3303-31-9
2219766
59, 60, 86
0.20% w/v
Met-Ala-Ser
307.37
17351-33-6
0.20% w/v
Ala-Ala-Ala
231.25
5874-90-8
2275380
0.20% w/v
Gly-Gly-Gly
189.17
556-33-2
2091220
0.20% w/v
Trp-Gly hydrochloride
297.74
63193-13-5
260.29
75277-39-3
2781697
2781697
43, 54, 85
0.05% w/v
L-Valine
117.15
72-18-4
2007736
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
55, 56
0.16% w/v
L-(+)-Arabinose
150.13
5328-37-0
0.16% w/v
DL-Glyceraldehyde
90.08
56-82-6
2002900
0.16% w/v
D-Glucose 6-phosphate
sodium salt
282.12
54010-71-8
2589210
0.16% w/v
D-(+)-Fucose
164.16
3615-37-0
2227929
51, 55
0.16% w/v
D-(+)-Mannose
180.16
3458-28-4
2223924
52, 55
0.16% w/v
Isomaltose
342.30
499-40-1
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
(E7)
59, 86
58, 59
Buffer
0.02 M
60
0.16% w/v
Gly-Gly-Gly-Gly
246.22
637-84-3
2113034
59, 60, 86
0.16% w/v
Leu-Gly-Gly
245.28
1187-50-4
2146981
0.16% w/v
Met-Ala-Ser
307.37
17351-33-6
0.16% w/v
Gly-Gly-Gly-Gly-Gly
303.28
7093-67-6
2303983
0.16% w/v
Ala-Leu
202.25
3303-34-2
2219771
58, 60
0.16% w/v
Tyr-Leu
294.35
17355-10-1
Buffer
0.02 M
HEPES sodium
pH 6.8
260.29
75277-39-3
Vanillin
152.15
121-33-5
2044652
D-Mannosamine hydrochloride
215.63
5505-63-5
2268478
0.04% w/v
D-(+)-Fucose
164.16
3615-37-0
2227929
48, 53
0.04% w/v
Guanine
151.13
73-40-5
2007998
59, 60, 86
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
60, 86
53, 55
(E5)
2781697
57, 60, 86
Silver Bullets Bio
-
Formulation Sheet (HR2-088)
Page 5 of 8
(E11)
HEPES sodium
pH 6.8
0.04% w/v
53
59
57, 58, 59
0.04% w/v
51, 53
(E10)
160.17
2004604
55
58
Trp-Gly hydrochloride
60-18-4
69477-27-6
57, 58
Ala-Ala
181.19
53, 55
0.02 M
0.16% w/v
L-Tyrosine
2781697
(E9)
(anhyd)
0.16% w/v
0.05% w/v
26, 33,
55, 94
57
MW
Buffer
58
2223924
499-40-1
(E8)
Silver Bullet Bio
57, 58, 59
22, 33, 44,
54, 66,
85, 93
50, 55
727.64
221.21
L-Leucine
0.02 M
0.04% w/v
N-Acetyl-Dmannosamine
0.05% w/v
Buffer
50, 53
0.16% w/v
43, 54, 85
(E6)
[units]
56
57, 86
43, 54, 85
Well #
49, 56
57, 58, 59
3458-28-4
Coproporphyrin I
dihydrochloride
2342142
2007982
180.16
75277-39-3
10597-89-4
2007453
D-(+)-Mannose
260.29
293.27
73-32-5
0.16% w/v
HEPES sodium pH 6.8
N-Acetylmuramic acid
71-00-1
51, 55
0.02 M
0.16% w/v
56
131.18
2268478
Buffer
2050231
155.16
5505-63-5
342.30
131-48-6
L-Isoleucine
215.63
(E4)
0.16% w/v
L-Histidine
D-Mannosamine
hydrochloride
52, 55
EC
309.27
0.05% w/v
0.16% w/v
52
CAS
N-Acetylneuraminic
acid
23, 36,
56, 93
0.05% w/v
42, 54, 85
51, 53
52, 62
54
Also in
60
(E12)
2781697
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
Well #
Wells F1 - F12
[units]
Silver Bullet Bio
MW
CAS
EC
61, 95
0.25% w/v
b-Nicotinamide adenine dinucleotide phosphate tetrasodium salt
833.35
2646-71-1
2201633
61, 73, 87, 88
0.25% w/v
Adenosine 5’-triphosphate disodium salt hydrate
551.15
987-65-5
0.25% w/v
N-Acetyl-D-galactosamine
221.21
1811-31-0
2173219
0.25% w/v
Gentamicin sulfate salt hydrate
1405-41-0
2157789
75277-39-3
2781697
61
13, 61
61
(F1)
Buffer
0.02 M
HEPES sodium pH 6.8
52, 62
0.20% w/v
Tryptone
260.29
Also in
[units]
Silver Bullet Bio
MW
CAS
EC
67, 81, 87
Well #
0.20% w/v
Thymine
126.11
65-71-4
2006161
63, 67
0.20% w/v
Sodium pyrophosphate tetrabasic decahydrate
446.06
13472-36-1
2317671
6, 67
67
0.20% w/v
D-Glyceric acid calcium salt dihydrate
143.12
14028-62-7
67, 69
(F7)
0.20% w/v
b-Cyclodextrin
1134.98
7585-39-9
2314932
67
0.20% w/v
myo-Inositol
180.16
87-89-8
2017812
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
0.20% w/v
Inosine 5’-monophosphate disodium salt
392.17
352195-40-5
2251464
68, 90
0.20% w/v
b-Nicotinamide adenine dinucleotide hydrate
663.43
53-84-9
2001844
62
0.20% w/v
D-Lactose monohydrate
360.31
64044-51-5
2005592
11, 68, 90
0.20% w/v
D-Fructose 1,6-diphosphate trisodium salt octahydrate
550.18
81028-91-3
2537780
(F2)
0.20% w/v
L-Citrulline
175.19
372-75-8
2067596
68, 94
68
0.20% w/v
Spermidine
145.25
124-20-9
2046890
62, 73
0.20% w/v
2’-Deoxycytidine 5’-monophosphate sodium salt
351.16
13085-50-2
2359952
68
(F8)
0.20% w/v
Adenosine 5’-diphosphoribose sodium salt
559.32
68414-18-6
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
35, 68
0.20% w/v
Histamine dihydrochloride
184.07
56-92-8
2002984
63, 67
0.20% w/v
Sodium pyrophosphate tetrabasic decahydrate
446.06
13472-36-1
2317671
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
38, 63
0.20% w/v
D-(−)-Ribose
150.13
50-69-1
2000594
69, 87
0.20% w/v
Pyridoxal 5-phosphate monohydrate
265.16
41468-25-1
0.20% w/v
Phytic acid sodium salt hydrate
660.04
14306-25-3
2382426
34, 69
15, 17, 62
62
1, 54, 62
41, 63
18, 19, 63
63
(F3)
0.20% w/v
63
0.04% w/v
Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate
(anhyd)
369.20
(anhyd)
37839-81-9
Acetyl coenzyme A sodium salt
809.58
102029-73-2
2533283
67, 69
30, 31, 69
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
64
0.25% w/v
S-(5’-Adenosyl)-L-methionine chloride
434.90
24346-00-7
2461848
0.25% w/v
Disodium beta-Glycerophosphate tetrahydrate
216.04
819-83-0
2124643
0.25% w/v
Stachyose hydrate
666.58
54261-98-2
2074273
70, 96
0.25% w/v
Cytidine 5’-diphosphocholine sodium salt dihydrate
546.34
33818-15-4
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
70, 92
15, 64
37, 64
64
(F4)
(anhyd)
Pyruvic acid
88.06
127-17-3
2048243
0.20% w/v
b-Cyclodextrin
1134.98
7585-39-9
2314932
(F9)
0.20% w/v
(±)-Epinephrine
183.21
329-65-7
2063476
0.20% w/v
myo-Inositol 2-monophosphate bis(cyclohexylammonium) salt
458.49
103529-92-6
69
Buffer
3, 64
0.20% w/v
69
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
33, 70
0.20% w/v
Adenosine 5’-diphosphate sodium salt
427.20
20398-34-9
70, 93
70
2781697
0.20% w/v
Adenosine 5’-monophosphate sodium salt
347.22
149022-20-8
70
0.20% w/v
Uridine 5’-monophosphate disodium salt hydrate
368.15
3387-36-8
2222119
(F10)
0.20% w/v
Cytidine 5’-diphosphate sodium salt hydrate
403.18
34393-59-4
2519908
0.20% w/v
Cytidine 5’-monophosphate disodium salt
367.16
6757-06-8
2298193
Buffer
0.02 M
65, 90
0.20% w/v
Biotin
244.31
58-85-5
2003993
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
36, 65
0.20% w/v
Phospho(enol)pyruvic acid monosodium salt hydrate
208.04
53823-68-0
2588107
71, 76
0.20% w/v
Pyridoxal hydrochloride
203.63
65-22-5
2006025
0.20% w/v
Sodium pyruvate
110.04
113-24-6
2040244
2115031
0.20% w/v
D-(+)-Melezitose hydrate
504.44
207511-10-2
209-894-9
65
0.20% w/v
Coenzyme B12
1579.61
13870-90-1
2376276
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
32, 66
0.20% w/v
Flavin adenine dinucleotide disodium salt hydrate
829.51
84366-81-4
2827338
66, 80
0.20% w/v
D-(−)-3-Phosphoglyceric acid disodium salt
230.02
80731-10-8
0.20% w/v
Spermine
202.34
71-44-3
0.20% w/v
Adenosine-5’-diphosphoglucose disodium salt
633.31
102129-65-7
65, 78
18, 65
66, 87
66
22, 33, 44, 54, 66,
85, 93
65
(F5)
66
(F6)
0.20% w/v
Buffer
Silver Bullets Bio
0.02 M
-
(anhyd)
2007542
71
2’-Deoxyadenosine 5’-monophosphate
331.23
653-63-4
71
0.20% w/v
Guanosine 5’-diphosphate sodium salt
443.20
43139-22-6
32, 71
(F11)
0.20% w/v
Nalidixic acid
232.24
389-08-2
2068647
0.20% w/v
Uridine 5’-diphospho-N-acetylglucosamine sodium salt
651.32
91183-98-1
2938205
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Guanosine 5’-triphosphate sodium salt hydrate
523.18
36051-31-7
2528472
0.25% w/v
Thymidine 5’-monophosphate disodium salt hydrate
366.18
33430-62-5
2515180
2007956
71, 77
Buffer
0.02 M
72, 89
0.25% w/v
30, 72
43, 54, 72, 85
L-Tyrosine
181.19
60-18-4
2004604
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Formulation Sheet (HR2-088)
0.20% w/v
71, 94
Page 6 of 8
72
(anhyd)
0.25% w/v
L-Tryptophan
204.23
73-22-3
31, 72
0.25% w/v
Inosine 5’-triphosphate trisodium salt
574.11
35908-31-7
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
(F12)
2781697
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
Well #
61, 73, 87, 88
62, 73
73
73, 90
73
(G1)
14, 34, 73
[units]
Silver Bullet Bio
MW
CAS
0.20% w/v
Adenosine 5’-triphosphate disodium salt hydrate
551.15
987-65-5
0.20% w/v
2’-Deoxycytidine 5’-monophosphate sodium salt
351.16
13085-50-2
0.20% w/v
b-Estradiol
272.38
50-28-2
0.20% w/v
D-(+)-Galactose
180.16
59-23-4
0.20% w/v
2'-Deoxyguanosine hydrate
267.24
961-07-9
Buffer
74
Buffer
0.02 M
74
10% v/v
(G2)
0.02 M
75, 88
0.20% w/v
75, 87
75
75, 79, 89
Wells G1 - G12
0.20% w/v
HEPES sodium pH 6.8
(anhyd)
EC
Also in
Silver Bullet Bio
MW
CAS
EC
80, 95
Well #
0.16% w/v
Riboflavin
376.36
83-88-5
2015071
2359952
9, 80
0.16% w/v
L-a-Phosphatidylcholine
8002-43-5
2323072
2000238
14, 80
0.16% w/v
D-(+)-Raffinose pentahydrate
594.52
17629-30-0
2081469
2004164
66, 80
0.16% w/v
D-(−)-3-Phosphoglyceric acid disodium salt
230.02
80731-10-8
0.16% w/v
g-Aminobutyric acid
103.12
56-12-2
2002586
0.16% w/v
Thionicotinamide adenine dinucleotide
679.50
4090-29-3
2238286
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
13, 25, 81, 91
0.16% w/v
2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate
347.22
0.16% w/v
Cholesterol
386.66
57-88-5
2003532
0.16% w/v
Thymine
126.11
65-71-4
2006161
0.16% w/v
Oxamic acid
89.05
471-47-6
2074430
260.29
75277-39-3
2781697
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
DL-a-Lipoic acid
206.33
1077-28-7
2140712
7, 81
2003066
67, 81, 87
Creatine monohydrate
149.15
6020-87-7
(G8)
80
75, 80, 84
Tacsimate pH 7.0
80
[units]
81
(anhyd)
75
0.20% w/v
L-Glutathione reduced
307.32
70-18-8
2007254
11, 81
(G3)
0.20% w/v
D-Pantothenic acid hemicalcium salt
238.27
137-08-6
2052789
52, 81
0.16% w/v
D-Glucosamine 6-phosphate sodium salt
259.15
70442-23-8
2002586
81
0.16% w/v
Guanosine 3’,5’-cyclic monophosphate sodium salt
367.19
40732-48-7
2550560
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
75, 80, 84
0.20% w/v
g-Aminobutyric acid
103.12
56-12-2
(G9)
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Buffer
71, 76
0.20% w/v
Pyridoxal hydrochloride
203.63
65-22-5
2006025
49, 82, 90
0.20% w/v
Thiamine monophosphate chloride dihydrate
416.82
273724-21-3
2085369
2027134
82, 89
0.20% w/v
Creatine phosphate disodium salt tetrahydrate
327.14
71519-72-7
2130746
6, 82
82
0.20% w/v
Na-Benzoyl-L-arginine ethyl ester hydrochloride
342.83
2645-08-1
2201570
(G10)
0.20% w/v
Tetracycline hydrochloride
480.90
64-75-5
2005938
0.20% w/v
Succinic acid disodium salt hexahydrate
270.14
6106-21-4
26, 76
76
27, 76
0.20% w/v
122.12
98-92-0
76
0.20% w/v
Batyl alcohol
344.57
544-62-7
2088747
(G4)
0.20% w/v
Glutaric acid
132.12
110-94-1
2038172
82
5, 82
28, 76
0.20% w/v
Buffer
0.02 M
77, 88
0.20% w/v
44, 77, 84
21, 77, 94
Nicotinamide
0.20% w/v
77
0.20% w/v
N-Acetyl-L-glutamic acid
189.17
1188-37-0
2147084
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2049886
83, 93
0.20% w/v
Uridine 5’-triphosphate trisodium salt hydrate
550.09
19817-92-6
2433475
2037425
1, 83
0.20% w/v
Kanamycin monosulfate
582.58
25389-94-0
2469339
83
0.20% w/v
Maltotriose hydrate
504.44
207511-08-8
2141742
(G11)
0.20% w/v
Leupeptin hydrochloride
463.02
24125-16-4
0.20% w/v
Sodium glyoxylate monohydrate
114.03
2706-75-4
2202988
2781697
Riboflavin 5’-monophosphate sodium salt dihydrate
Maleic acid
Pyridoxamine dihydrochloride
514.36
116.07
241.11
6184-17-4
110-16-7
524-36-7
2083576
3, 83
0.20% w/v
Acetylcholine chloride
181.66
60-31-1
2004688
83
71, 77
0.20% w/v
Uridine 5’-diphospho-N-acetylglucosamine sodium salt
651.32
91183-98-1
2938205
4, 83
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
0.16% w/v
5-Phospho-D-ribose 1-diphosphate pentasodium salt
499.98
108321-05-7
0.16% w/v
Maleic acid
116.07
110-16-7
2037425
0.16% w/v
N-Acetyl-D-glucosamine
221.21
7512-17-6
2313682
0.16% w/v
L-(−)-Malic acid sodium salt
156.07
68303-40-2
0.16% w/v
g-Aminobutyric acid
103.12
56-12-2
2002586
Acetylcholine chloride
181.66
60-31-1
2004688
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
77, 84
(G5)
23, 78
0.20% w/v
Fumaric acid
116.07
110-17-8
2037430
84
24, 78
0.20% w/v
cis-Aconitic acid
174.11
585-84-2
2095644
44, 77, 84
0.20% w/v
DL-Isocitric acid trisodium salt
258.07
1637-73-6
2166683
56, 84, 89
78
78
84
0.20% w/v
Oxalacetic acid
132.07
328-42-7
2063298
84, 87
65, 78
0.20% w/v
Sodium pyruvate
110.04
113-24-6
2040244
75, 80, 84
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
77, 84
0.16% w/v
Buffer
0.02 M
25, 78
(G6)
79, 89
0.04% w/v
b-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate
766.40
1184-16-3
2146646
10, 79
0.04% w/v
Tetrahydrofolic acid
445.43
135-16-0
2051811
0.04% w/v
L-Ascorbic acid
176.12
50-81-7
2000662
0.04% w/v
D-(+)-Glucose
180.16
50-99-7
2000751
0.04% w/v
Folic acid
441.40
59-30-3
2004190
75, 79, 89
0.04% w/v
D-Pantothenic acid hemicalcium salt
238.27
137-08-6
2052789
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
41, 79
20, 79
79
79
(G7)
Silver Bullets Bio
-
Formulation Sheet (HR2-088)
(anhyd)
Page 7 of 8
(G12)
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
Also in
[units]
Silver Bullet
CAS
EC
44, 54, 85
Well #
0.05% w/v
Glycine
75.07
56-40-6
2002722
44, 54, 85
0.05% w/v
L-(-)-Threonine
119.12
72-19-5
2007741
42, 54, 85
0.05% w/v
L-(+)-Lysine
146.19
56-87-1
2002942
43, 54, 85
0.05% w/v
L-Alanine
89.09
56-41-7
2002738
1,10, 21,42, 54, 85
0.05% w/v
L-Arginine
174.20
74-79-3
2008111
44, 54, 85
0.05% w/v
L-Asparagine monohydrate
150.13
5794-13-8
2007359
41, 42, 54, 85
0.05% w/v
L-Aspartic acid
133.10
56-84-8
2002916
10, 42, 54, 85
0.05% w/v
L-Glutamic acid
147.13
56-86-0
2002937
44, 54, 85
42, 54, 85
43, 54, 85
0.05% w/v
L-Glutamine
MW
146.15
56-85-9
2002921
85
0.05% w/v
L-Histidine
155.16
71-00-1
2007453
(H1)
0.05% w/v
L-Isoleucine
131.18
73-32-5
2007982
0.05% w/v
L-Leucine
131.18
61-90-5
2005220
43, 54, 85
Wells H1 - H12
Also in
Guanosine 5’-triphosphate
sodium salt hydrate
523.18
36051-31-7
2528472
0.16% w/v
Uridine
244.20
58-96-8
2004075
22, 33, 44,
54, 66,
85, 93
0.16% w/v
D-Pantothenic acid
hemicalcium salt
238.27
137-08-6
2052789
2313682
75, 79, 89
56, 84, 89
0.16% w/v
Buffer
0.02 M
49, 82, 90
N-Acetyl-D-glucosamine
221.21
7512-17-6
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
0.14% w/v
Thiamine monophosphate
chloride dihydrate
416.82
273724-21-3
2085369
663.43
53-84-9
2001844
L-Phenylalanine
165.19
63-91-2
2005681
18, 33, 90
0.14% w/v
Guanosine
283.24
118-00-3
2042278
43, 54, 85
0.05% w/v
L-Proline
115.13
147-85-3
2057022
19, 35, 90
0.14% w/v
Thymidine
242.23
50-89-5
73, 90
0.14% w/v
D-(+)-Galactose
180.16
59-23-4
2007956
22, 33, 44, 54, 66, 85, 93
0.05% w/v
L-Tyrosine
181.19
60-18-4
2004604
43, 54, 85
0.05% w/v
L-Valine
117.15
72-18-4
2007736
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
60, 86
0.14% w/v
Gly-Gly-Gly-Gly-Gly
303.28
7093-67-6
59, 60, 86
59, 86
59, 60, 86
57, 86
0.14% w/v
Met-Ala-Ser
307.37
17351-33-6
(H2)
0.14% w/v
Val-Ser
204.23
13588-94-8
57, 60, 86
0.14% w/v
Ala-Leu
202.25
3303-34-2
58, 86
0.14% w/v
Z-Val-Phe
398.46
19542-51-9
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2219771
65-71-4
2006161
84, 87
0.16% w/v
L-(−)-Malic acid sodium salt
156.07
68303-40-2
66, 87
0.16% w/v
Spermine
202.34
71-44-3
2007542
15, 16, 92
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
2, 18, 92
61, 73, 87, 88
0.16% w/v
Adenosine 5’-triphosphate disodium
salt hydrate
551.15
987-65-5
77, 88
0.16% w/v
Riboflavin 5’-monophosphate sodium
salt dihydrate
514.36
6184-17-4
2049886
88
0.16% w/v
DL-a-Lipoic acid
206.33
1077-28-7
2140712
(H4)
75, 88
26, 40, 49, 88
0.16% w/v
Acetylsalicylic acid
180.16
50-78-2
2000641
0.16% w/v
L-Ornithine hydrochloride
168.62
3184-13-2
2216786
22, 31, 88
0.16% w/v
Adenosine
267.24
58-61-7
2003899
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
Silver Bullets Bio
-
Formulation Sheet (HR2-088)
0.16% w/v
Guanosine 5’diphosphate sodium salt
443.20
43139-22-6
0.16% w/v
Cytidine 5’-triphosphate
disodium salt
527.12
36051-68-0
2528493
0.16% w/v
D-Glucose 6-phosphate
sodium salt
282.12
54010-71-8
2589210
2781697
197.66
6645-46-1
2296636
75277-39-3
2781697
0.16% w/v
2’-Deoxyguanosine 5’monophosphate sodium salt
hydrate
347.22
0.16% w/v
Thymidine 5’-triphosphate
sodium salt
504.15
18423-43-3
0.16% w/v
4-Aminobenzoic acid
137.14
150-13-0
2, 12, 95
0.16% w/v
61, 95
0.16% w/v
b-Nicotinamide adenine
dinucleotide phosphate
tetrasodium salt
833.35
2646-71-1
2201633
0.16% w/v
2’-Deoxyadenosine
5’-triphosphate disodium
salt
535.15
1927-31-7
2176623
0.16% w/v
Choline base solution
121.18
123-41-1
23, 38, 95
0.16% w/v
a-D-Glucose
1-phosphate disodium
salt hydrate
304.10
80, 95
0.16% w/v
Riboflavin
Buffer
0.02 M
HEPES sodium pH 6.8
(anhyd)
12, 26, 95
2057530
2781697
26, 39, 95
95
(H11)
56401-20-8
2601541
376.36
83-88-5
2015071
260.29
75277-39-3
2781697
368.15
3387-36-8
2222119
(anhyd)
70, 96
0.16% w/v
Uridine 5’monophosphate
disodium salt hydrate
8, 96
0.16% w/v
D-(+)-Maltose
monohydrate
360.32
6363-53-7
2007165
0.16% w/v
D-Sorbitol
182.17
50-70-4
2000615
0.16% w/v
O-Phospho-L-serine
185.07
407-41-0
2069860
0.16% w/v
Benzidine
184.24
92-87-5
2021991
27, 31, 96
0.16% w/v
Sodium phenyl
phosphate dibasic
dihydrate
254.09
66778-08-3
2219174
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
75277-39-3
0.16% w/v
741.63
104809-32-7
0.16% w/v
Guanidine hydrochloride
95.53
50-01-1
2000023
9, 14, 96
0.16% w/v
Cystamine dihydrochloride
225.20
56-17-7
2002607
4, 13, 96
0.16% w/v
Aspartame
294.30
22839-47-0
2452613
12, 96
2, 17, 92
0.16% w/v
Caffeine
194.19
58-08-2
2003621
70, 92
0.16% w/v
Cytidine 5’-monophosphate
disodium salt
367.16
6757-06-8
2298193
Buffer
0.02 M
HEPES sodium pH 6.8
260.29
75277-39-3
2781697
(H8)
2083576
L-Carnitine
hydrochloride
260.29
92
524-36-7
2046890
b-Nicotinamide adenine
dinucleotide, reduced
dipotassium salt
92
1, 88
71, 94
HEPES sodium pH 6.8
91, 92
241.11
75277-39-3
104809-32-7
126.11
21, 77, 94
124-20-9
741.63
Thymine
(H3)
2781697
Pyridoxamine
dihydrochloride
0.16% w/v
260.29
b-Nicotinamide adenine
dinucleotide, reduced
dipotassium salt
0.16% w/v
67, 81, 87
2001011
75277-39-3
HEPES sodium pH 6.8
260.29
0.02 M
51-48-9
260.29
0.02 M
0.16% w/v
Buffer
776.87
HEPES sodium pH 6.8
Buffer
91, 92
2003066
L-Thyroxine
2003993
2167954
6020-87-7
2004604
21820-51-9
1670-14-0
41468-25-1
60-18-4
145.25
156.61
149.15
181.19
261.17
Benzamidine hydrochloride
265.16
2050231
Spermidine
0.16% w/v
Creatine monohydrate
131-48-6
(anhyd)
(H9)
O-Phospho-L-tyrosine
7, 11, 91
Pyridoxal 5-phosphate monohydrate
87
309.27
0.16% w/v
2007495
0.16% w/v
75, 87
149022-20-8
0.16% w/v
71-30-7
0.16% w/v
69, 87
987-65-5
347.22
68, 94
111.10
551.15
0.16% w/v
2433475
3, 17, 94
HEPES sodium pH 6.8
2781697
19817-92-6
2004164
Cytosine
(H7)
550.09
(H10)
0.16% w/v
Adenosine 5’-triphosphate disodium
salt hydrate
61, 73, 87, 88
91
EC
26, 33,
55, 94
2537780
14, 24, 91
12, 28,
37, 38, 91
58-85-5
CAS
2000704
81028-91-3
13, 25,
81, 91
13, 19, 91
244.31
93
MW
94
550.18
2303983
2275380
5874-90-8
Biotin
23, 36,
56, 93
Well #
15, 94
D-Fructose 1,6-diphosphate
trisodium salt octahydrate
0.02 M
2146981
231.25
0.14% w/v
Buffer
1187-50-4
Ala-Ala-Ala
65, 90
2781697
245.28
0.14% w/v
(H6)
0.14% w/v
Leu-Gly-Gly
86
90
11, 68, 90
0.14% w/v
0.02 M
0.16% w/v
0.05% w/v
2002743
0.20% w/v
Buffer
2146646
29, 43, 54, 85
73-22-3
27, 41, 93
1184-16-3
0.14% w/v
56-45-1
L-Tyrosine
766.40
68, 90
204.23
0.20% w/v
b-Nicotinamide adenine
dinucleotide phosphate
sodium salt hydrate
2005629
105.09
N-Acetylneuraminic acid
0.16% w/v
63-68-3
L-Tryptophan
0.20% w/v
79, 89
149.21
L-Serine
Adenosine 5’-monophosphate sodium salt
2130746
L-Methionine
0.05% w/v
0.20% w/v
71519-72-7
0.05% w/v
0.05% w/v
70, 93
327.14
43, 54, 85
44, 54, 85
0.20% w/v
0.16% w/v
b-Nicotinamide adenine
dinucleotide hydrate
43, 54, 72, 85
83, 93
Uridine 5’-triphosphate
trisodium salt hydrate
82, 89
(H5)
EC
Silver Bullet
Creatine phosphate disodium
salt tetrahydrate
13, 36, 89
CAS
[units]
Silver Bullet
89
MW
Also in
[units]
72, 89
Well #
96
(H12)
Hampton Research
34 Journey
Aliso Viejo, CA 92656-3317 U.S.A.
Tel: (949) 425-1321 • Fax: (949) 425-1611
Technical Support e-mail: [email protected]
Website: www.hamptonresearch.com
Page 8 of 8
© 1991-2015 Hampton Research Corp.
silver bullets bio
TM
HR2-088 - Scoring Sheet
Sample:
Sample Concentration:
Sample Buffer:
Date:
Reservoir Volume:
Drop Volume: Total
A1 - A12
Temperature:
ml
Sample
ml
Reservoir
ml
Additive
ml
1
2
3
4
Silver Bullets Bio
Date
1
(A1)
0.16% w/v L-Citrulline, 0.16% w/v L-Ornithine hydrochloride, 0.16% w/v Urea, 0.16% w/v Oxalic acid, 0.16% w/v Kanamycin monosulfate,
0.16% w/v L-Arginine, 0.02 M HEPES sodium pH 6.8
2
(A2)
0.16% w/v L-Carnitine hydrochloride, 0.16% w/v Tannic acid, 0.16% w/v Aspartame, 0.16% w/v Caffeine, 0.16% w/v p-Coumaric acid,
0.16% w/v 4-Hydroxy-L-proline, 0.02 M HEPES sodium pH 6.8
3
(A3)
0.16% w/v Disodium beta-Glycerophosphate tetrahydrate, 0.16% w/v trans-Cinnamic acid, 0.16% w/v O-Phospho-L-tyrosine,
0.16% w/v Betaine anhydrous, 0.16% w/v Maltotriose hydrate, 0.16% w/v Cytidine, 0.02 M HEPES sodium pH 6.8
4
(A4)
0.16% w/v L-Canavanine, 0.16% w/v O-Phospho-L-serine, 0.16% w/v Taurine, 0.16% w/v Quinine, 0.16% w/v Sodium glyoxylate monohydrate,
0.16% w/v Cholic acid, 0.02 M HEPES sodium pH 6.8
5
(A5)
0.08% w/v Ellipticine, 0.20% w/v Gibberellin A 3 , 0.20% w/v trans-Cinnamic acid, 0.20% w/v Phenol, 0.20% w/v Succinic acid disodium salt hexahydrate,
0.02 M HEPES sodium pH 6.8
6
(A6)
0.16% w/v Sulfanilamide, 0.16% w/v D-(+)-Cellobiose, 0.16% w/v D-Glyceric acid calcium salt dihydrate, 0.16% w/v 6-Phosphogluconic acid trisodium salt,
0.16% w/v Na-Benzoyl-L-arginine ethyl ester hydrochloride, 0.16% w/v D-(+)-Galactosamine hydrochloride, 0.02 M HEPES sodium pH 6.8
7
(A7)
0.16% w/v Benzamidine hydrochloride, 0.16% w/v Acarbose, 0.16% w/v Ethidium bromide, 0.16% w/v Cholesterol, 0.16% w/v Sarcosine,
0.16% w/v L-Homoserine, 0.02 M HEPES sodium pH 6.8
8
(A8)
0.20% w/v Sodium gluconate, 0.20% w/v Acarbose, 0.20% w/v D-(+)-Maltose monohydrate, 0.20% w/v Salicylic acid,
0.20% w/v D-(+)-Melibiose monohydrate, 0.02 M HEPES sodium pH 6.8
9
(A9)
0.20% w/v g-Strophanthin, 0.20% w/v D-Sorbitol, 0.20% w/v 1,4-Diaminobutane, 0.20% w/v D-(+)-Trehalose dihydrate,
0.20% w/v L-a-Phosphatidylcholine, 0.02 M HEPES sodium pH 6.8
10
(A10)
0.16% w/v Sucrose, 0.16% w/v Cadaverine, 0.16% w/v L-Glutamic acid, 0.16% w/v L-Arginine, 0.16% w/v Oxalic acid, 0.16% w/v Tetrahydrofolic acid,
0.02 M HEPES sodium pH 6.8
11
(A11)
0.20% w/v Protamine sulfate salt, 0.20% w/v g-Strophanthin, 0.20% w/v Benzamidine hydrochloride,
0.20% w/v D-Fructose 1,6-diphosphate trisodium salt octahydrate, 0.20% w/v Oxamic acid, 0.02 M HEPES sodium pH 6.8
12
(A12)
0.20% w/v 2’-Deoxyadenosine 5’-triphosphate disodium salt, 0.20% w/v Benzidine, 0.20% w/v L-Carnitine hydrochloride, 0.20% w/v Sulfanilamide,
0.20% w/v Cytosine, 0.02 M HEPES sodium pH 6.8
B1 - B12
Silver Bullets Bio
Date
13
(B1)
0.20% w/v 2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate, 0.20% w/v N-Acetyl-D-galactosamine, 0.20% w/v O-Phospho-L-serine,
0.20% w/v 4-Aminobenzoic acid, 0.20% w/v Uridine, 0.02 M HEPES sodium pH 6.8
14
(B2)
0.16% w/v Thymidine 5’-triphosphate sodium salt, 0.16% w/v L-Canavanine, 0.16% w/v D-Sorbitol, 0.16% w/v Salicylic acid, 0.16% w/v 2’-Deoxyguanosine hydrate,
0.16% w/v D-(+)-Raffinose pentahydrate, 0.02 M HEPES sodium pH 6.8
15
(B3)
0.16% w/v Cytidine 5’-triphosphate disodium salt, 0.16% w/v Stachyose hydrate, 0.16% w/v Cystathionine, 0.16% w/v Cystamine dihydrochloride,
0.16% w/v trans-Cinnamic acid, 0.16% w/v Inosine 5’-monophosphate disodium salt, 0.02 M HEPES sodium pH 6.8
16
(B4)
0.20% w/v Uridine 5’-diphosphate sodium salt, 0.20% w/v Quinine, 0.20% w/v p-Coumaric acid, 0.20% w/v D-(+)-Melibiose monohydrate,
0.20% w/v Cystamine dihydrochloride, 0.02 M HEPES sodium pH 6.8
17
(B5)
0.20% w/v Nicotinic acid, 0.20% w/v Inosine 5’-monophosphate disodium salt, 0.20% w/v Gibberellin A 3 , 0.20% w/v O-Phospho-L-tyrosine, 0.20% w/v Caffeine,
0.02 M HEPES sodium pH 6.8
18
(B6)
0.20% w/v Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate, 0.20% w/v Cadaverine, 0.20% w/v D-(+)-Melezitose hydrate, 0.20% w/v Aspartame,
0.20% w/v Guanosine, 0.02 M HEPES sodium pH 6.8
19
(B7)
0.16% w/v Thymidine, 0.16% w/v Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate, 0.16% w/v Sarcosine, 0.16% w/v 4-Aminobenzoic acid,
0.16% w/v Acarbose, 0.16% w/v Inosine, 0.02 M HEPES sodium pH 6.8
20
(B8)
0.08% w/v Ellipticine, 0.20% w/v Protamine sulfate salt, 0.20% w/v D-(+)-Trehalose dihydrate, 0.20% w/v 6-Phosphogluconic acid trisodium salt,
0.20% w/v D-(+)-Glucose, 0.02 M HEPES sodium pH 6.8
21
(B9)
0.20% w/v Penicillin G sodium salt, 0.20% w/v L-Arginine, 0.20% w/v D-(+)-Cellobiose, 0.20% w/v Pyridoxamine dihydrochloride, 0.20% w/v Betaine anhydrous,
0.02 M HEPES sodium pH 6.8
22
(B10)
0.20% w/v 2’-Deoxyadenosine monohydrate, 0.20% w/v N-Acetylneuraminic acid, 0.20% w/v N-p-Tosyl-L-phenylalanine chloromethyl ketone,
0.20% w/v a-D-Glucose 1-phosphate disodium salt hydrate, 0.20% w/v Fumaric acid, 0.02 M HEPES sodium pH 6.8
24
0.25% w/v Thymidine 5’-triphosphate sodium salt, 0.25% w/v a-Ketoglutaric acid disodium salt, 0.25% w/v 2-Nitrophenyl b-D-galactopyranoside,
0.25% w/v cis-Aconitic acid, 0.02 M HEPES sodium pH 6.8
(B12)
Posettes or Spherulites
Needles (1D Growth)
Plates (2D Growth)
Single Crystals (3D Growth < 0.2 mm)
Single Crystals (3D Growth > 0.2 mm)
Date
Date
Date
Date
Date
Date
0.20% w/v Nicotinic acid, 0.20% w/v Adenosine, 0.20% w/v L-Tyrosine, 0.20% w/v Xanthine, 0.20% w/v L-Lactic acid, 0.02 M HEPES sodium pH 6.8
23
(B11)
5
6
7
8
9
Clear Drop
Phase Separation
Regular Granular Precipitate
Birefringent Precipitate or
Microcrystals
Scoring Sheet - HR2-088
Page 1 of 4
© 1991 - 2015 Hampton Research Corp.
silver bullets bio
TM
HR2-088 - Scoring Sheet
Sample:
Sample Concentration:
Sample Buffer:
Date:
Reservoir Volume:
Drop Volume: Total
C1 - C12
Temperature:
ml
Sample
ml
Reservoir
ml
Additive
ml
1
2
3
4
Clear Drop
Phase Separation
Regular Granular Precipitate
Birefringent Precipitate or
Microcrystals
Silver Bullets Bio
25
(C1)
0.20% w/v 2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate, 0.20% w/v Ethanolamine, 0.20% w/v Theophylline,
0.20% w/v Isopropyl 1-thio-b-D-galactopyranoside, 0.20% w/v Oxalacetic acid, 0.02 M HEPES sodium pH 6.8
26
(C2)
0.20% w/v 2’-Deoxyadenosine 5’-triphosphate disodium salt, 0.20% w/v D-Glucose 6-phosphate sodium salt, 0.20% w/v Acetylsalicylic acid,
0.20% w/v Choline base solution, 0.20% w/v Nicotinamide, 0.02 M HEPES sodium pH 6.8
27
(C3)
0.20% w/v Uridine 5’-diphosphate sodium salt, 0.20% w/v L-Carnosine, 0.20% w/v L-Thyroxine, 0.20% w/v Sodium phenyl phosphate dibasic dihydrate,
0.20% w/v Glutaric acid, 0.02 M HEPES sodium pH 6.8
28
(C4)
0.20% w/v Cytosine, 0.20% w/v Vitamin B12, 0.20% w/v Pepstatin A, 0.20% w/v Shikimic acid, 0.20% w/v N-Acetyl-L-glutamic acid,
0.02 M HEPES sodium pH 6.8
29
(C5)
0.20% w/v 2’-Deoxycytidine 5’-triphosphate disodium salt, 0.20% w/v Hydrocortisone, 0.20% w/v Hemin, 0.20% w/v O-Phospho-DL-threonine,
0.20% w/v L-Phenylalanine, 0.02 M HEPES sodium pH 6.8
30
(C6)
0.04% w/v Cortisone, 0.04% w/v (±)-Epinephrine, 0.04% w/v Protoporphyrin disodium salt, 0.04% w/v Pyridoxine,
0.04% w/v Thymidine 5’-monophosphate disodium salt hydrate, 0.02 M HEPES sodium pH 6.8
31
(C7)
0.20% w/v Adenosine, 0.20% w/v Pepstatin A, 0.20% w/v (±)-Epinephrine, 0.20% w/v Sodium phenyl phosphate dibasic dihydrate,
0.20% w/v Inosine 5’-triphosphate trisodium salt, 0.02 M HEPES sodium pH 6.8
32
(C8)
0.20% w/v 2’-Deoxyadenosine monohydrate, 0.20% w/v O-Phospho-DL-threonine, 0.20% w/v Flavin adenine dinucleotide disodium salt hydrate,
0.20% w/v DL-Xylose, 0.20% w/v Nalidixic acid, 0.02 M HEPES sodium pH 6.8
33
(C9)
0.20% w/v Guanosine, 0.20% w/v D-Glucose 6-phosphate sodium salt, 0.20% w/v 2,3-Pyridinedicarboxylic acid, 0.20% w/v L-Tyrosine,
0.20% w/v Adenosine 5’-diphosphate sodium salt, 0.02 M HEPES sodium pH 6.8
34
(C10)
0.25% w/v 2’-Deoxyguanosine hydrate, 0.25% w/v Cortisone, 0.25% w/v a-Ketoglutaric acid disodium salt, 0.25% w/v Pyruvic acid,
0.02 M HEPES sodium pH 6.8
35
(C11)
0.20% w/v Thymidine, 0.20% w/v trans-Dehydroandrosterone, 0.20% w/v Xanthine, 0.20% w/v Shikimic acid, 0.20% w/v Histamine dihydrochloride,
0.02 M HEPES sodium pH 6.8
36
(C12)
0.20% w/v Uridine, 0.20% w/v Hydrocortisone, 0.20% w/v N-Acetylneuraminic acid, 0.20% w/v 2-Nitrophenyl b-D-galactopyranoside,
0.20% w/v Phospho(enol)pyruvic acid monosodium salt hydrate, 0.02 M HEPES sodium pH 6.8
D1 - D12
Silver Bullets Bio
37
(D1)
0.20% w/v Cytosine, 0.20% w/v Pyridoxine, 0.20% w/v Hemin, 0.20% w/v L-Carnosine, 0.20% w/v Cytidine 5’-diphosphocholine sodium salt dihydrate,
0.02 M HEPES sodium pH 6.8
38
(D2)
0.20% w/v Cytosine, 0.20% w/v a-D-Glucose 1-phosphate disodium salt hydrate, 0.20% w/v trans-Dehydroandrosterone,
0.20% w/v Isopropyl 1-thio-b-D-galactopyranoside, 0.20% w/v D-(−)-Ribose, 0.02 M HEPES sodium pH 6.8
39
(D3)
0.20% w/v Thiamine pyrophosphate, 0.20% w/v D-Glucosamic acid, 0.20% w/v Choline base solution, 0.20% w/v Theophylline, 0.20% w/v Ethanolamine,
0.02 M HEPES sodium pH 6.8
40
(D4)
0.20% w/v Acetylsalicylic acid, 0.20% w/v Vitamin B12, 0.20% w/v 2,3-Pyridinedicarboxylic acid, 0.20% w/v Ethanolamine, 0.20% w/v Thiamine hydrochloride,
0.02 M HEPES sodium pH 6.8
41
(D5)
0.16% w/v L-Lactic acid, 0.16% w/v L-Aspartic acid, 0.16% w/v L-Thyroxine, 0.16% w/v Pyridoxine, 0.16% w/v L-Ascorbic acid,
0.16% w/v Phytic acid sodium salt hydrate, 0.02 M HEPES sodium pH 6.8
42
(D6)
0.16% w/v Thiamine pyrophosphate, 0.16% w/v L-Aspartic acid, 0.16% w/v L-Glutamic acid, 0.16% w/v L-(+)-Lysine, 0.16% w/v L-Arginine, 0.16% w/v L-Histidine,
0.02 M HEPES sodium pH 6.8
43
(D7)
0.125% w/v L-Methionine, 0.125% w/v L-Phenylalanine, 0.125% w/v L-Leucine, 0.125% w/v L-Isoleucine, 0.125% w/v L-Valine, 0.125% w/v L-Alanine,
0.125% w/v L-Tryptophan, 0.125% w/v L-Proline, 0.02 M HEPES sodium pH 6.8
44
(D8)
0.14% w/v Glycine, 0.14% w/v L-Asparagine monohydrate, 0.14% w/v L-Glutamine, 0.14% w/v L-Tyrosine, 0.14% w/v L-Serine,
0.14% w/v L-(-)-Threonine, 0.14% w/v Maleic acid, 0.02 M HEPES sodium pH 6.8
45
(D9)
0.14% w/v Sodium bromide, 0.14% w/v Sodium fluoride, 0.14% w/v Sodium carbonate, 0.14% w/v Sodium tetraborate decahydrate,
0.14% w/v Sodium phosphate dibasic dihydrate, 0.14% w/v Sodium nitrate, 0.14% w/v Rhenium(IV) oxide, 0.02 M HEPES sodium pH 6.8
46
(D10)
0.002 M Calcium chloride dihydrate, 0.002 M Cadmium chloride hydrate, 0.002 M Magnesium chloride hexahydrate,
0.002 M Manganese(II) chloride tetrahydrate, 0.002 M Zinc chloride, 0.02 M HEPES sodium pH 6.8
47
(D11)
0.002 M Nickel(II) chloride hexahydrate, 0.002 M Copper(II) chloride dihydrate, 0.002 M Cobalt(II) chloride hexahydrate, 0.002 M Molybdenum(III) chloride.
0.02 M HEPES sodium pH 6.8
48
(D12)
0.16% w/v L-Homoserine, 0.16% w/v 4-Hydroxy-L-proline, 0.16% w/v Argininosuccinic acid disodium salt hydrate, 0.16% w/v Cytidine,
0.16% w/v Inosine, 0.16% w/v Guanine, 0.02 M HEPES sodium pH 6.8
Scoring Sheet - HR2-088
Page 2 of 4
5
6
7
8
9
Posettes or Spherulites
Needles (1D Growth)
Plates (2D Growth)
Single Crystals (3D Growth < 0.2 mm)
Single Crystals (3D Growth > 0.2 mm)
Date
Date
Date
Date
Date
Date
Date
Date
© 1991 - 2015 Hampton Research Corp.
silver bullets bio
TM
HR2-088 - Scoring Sheet
Sample:
Sample Concentration:
Sample Buffer:
Date:
Reservoir Volume:
Drop Volume: Total
E1 - E12
Temperature:
ml
Sample
ml
Reservoir
ml
Additive
ml
1
2
3
4
Clear Drop
Phase Separation
Regular Granular Precipitate
Birefringent Precipitate or
Microcrystals
Silver Bullets Bio
49
(E1)
0.16% w/v Thiamine monophosphate chloride dihydrate, 0.16% w/v Acetylsalicylic acid, 0.16% w/v Cholic acid, 0.16% w/v 1,2,3-Heptanetriol, 0.16% w/v Vanillin,
50
(E2)
0.20% w/v Adenosine 5’-(b,g-imido)triphosphate tetralithium salt hydrate, 0.20% w/v Protoporphyrin disodium salt, 0.20% w/v Coproporphyrin I dihydrochloride,
51
(E3)
0.16% w/v D-Glucosamic acid, 0.16% w/v D-(+)-Galactosamine hydrochloride, 0.16% w/v D-(+)-Glucosamine hydrochloride, 0.16% w/v Sodium gluconate,
52
(E4)
53
(E5)
5
6
7
8
9
Posettes or Spherulites
Needles (1D Growth)
Plates (2D Growth)
Single Crystals (3D Growth < 0.2 mm)
Single Crystals (3D Growth > 0.2 mm)
Date
Date
Date
Date
Date
Date
Date
Date
0.16% w/v N-Acetyl-D-mannosamine, 0.02 M HEPES sodium pH 6.8
0.20% w/v Tris, 0.20% w/v DL-Glyceraldehyde, 0.02 M HEPES sodium pH 6.8
0.16% w/v D-Mannosamine hydrochloride, 0.16% w/v D-(+)-Mannose, 0.02 M HEPES sodium pH 6.8
0.16% w/v D-Glucosamine 6-phosphate sodium salt, 1.00% w/v Tryptone, 0.16% w/v Isomaltose, 0.02 M HEPES sodium pH 6.8
0.04% w/v Coproporphyrin I dihydrochloride, 0.04% w/v Vanillin, 0.04% w/v D-Mannosamine hydrochloride, 0.04% w/v D-(+)-Fucose,
0.04% w/v Guanine, 0.02 M HEPES sodium pH 6.8
0.05% w/v L-Citrulline, 0.05% w/v Glycine, 0.05% w/v L-(-)-Threonine, 0.05% w/v L-(+)-Lysine, 0.05% w/v L-Alanine, 0.05% w/v L-Arginine,
54
(E6)
0.05% w/v L-Asparagine monohydrate, 0.05% w/v L-Aspartic acid, 0.05% w/v L-Glutamic acid, 0.05% w/v L-Glutamine, 0.05% w/v L-Histidine,
0.05% w/v L-Isoleucine, 0.05% w/v L-Leucine, 0.05% w/v L-Methionine, 0.05% w/v L-Phenylalanine, 0.05% w/v L-Proline, 0.05% w/v L-Serine,
0.05% w/v L-Tryptophan, 0.05% w/v L-Tyrosine, 0.05% w/v L-Valine, 0.02 M HEPES sodium pH 6.8
55
(E7)
0.16% w/v L-(+)-Arabinose, 0.16% w/v DL-Glyceraldehyde, 0.16% w/v D-Glucose 6-phosphate sodium salt, 0.16% w/v D-(+)-Fucose, 0.16% w/v D-(+)-Mannose,
56
(E8)
0.16% w/v N-Acetylneuraminic acid, 0.16% w/v N-Acetylmuramic acid, 0.16% w/v N-Acetyl-D-mannosamine, 0.16% w/v N-Acetyl-D-glucosamine, 0.16% w/v Tris,
0.16% w/v Isomaltose, 0.02 M HEPES sodium pH 6.8
0.16% w/v L-(+)-Arabinose, 0.02 M HEPES sodium pH 6.8
57
(E9)
0.16% w/v Tyr-Phe, 0.16% w/v Leu-Leu, 0.16% w/v Ala-Leu, 0.16% w/v Val-Ser, 0.16% w/v Trp-Gly hydrochloride, 0.16% w/v Met-Gly, 0.02 M HEPES sodium pH 6.8
58
(E10)
0.16% w/v Tyr-Phe, 0.16% w/v Tyr-Leu, 0.16% w/v Z-Val-Phe, 0.16% w/v Gly-Gly-Gly, 0.16% w/v Ala-Ala, 0.16% w/v Trp-Gly hydrochloride,
0.02 M HEPES sodium pH 6.8
59
(E11)
0.20% w/v Leu-Gly-Gly, 0.20% w/v Leu-Leu, 0.20% w/v Met-Ala-Ser, 0.20% w/v Ala-Ala-Ala, 0.20% w/v Gly-Gly-Gly, 0.20% w/v Trp-Gly hydrochloride,
60
(E12)
0.16% w/v Gly-Gly-Gly-Gly, 0.16% w/v Leu-Gly-Gly, 0.16% w/v Met-Ala-Ser, 0.16% w/v Gly-Gly-Gly-Gly-Gly, 0.16% w/v Ala-Leu, 0.16% w/v Tyr-Leu,
F1 - F12
0.02 M HEPES sodium pH 6.8
0.02 M HEPES sodium pH 6.8
Silver Bullets Bio
61
(F1)
0.25% w/v b-Nicotinamide adenine dinucleotide phosphate tetrasodium salt, 0.25% w/v Adenosine 5’-triphosphate disodium salt hydrate,
62
(F2)
0.20% w/v Tryptone, 0.20% w/v Inosine 5’-monophosphate disodium salt, 0.20% w/v D-Lactose monohydrate, 0.20% w/v L-Citrulline,
63
(F3)
0.20% w/v Sodium pyrophosphate tetrabasic decahydrate, 0.20% w/v D-(−)-Ribose, 0.20% w/v Phytic acid sodium salt hydrate,
64
(F4)
0.25% w/v S-(5’-Adenosyl)-L-methionine chloride, 0.25% w/v Disodium beta-Glycerophosphate tetrahydrate, 0.25% w/v Stachyose hydrate,
0.25% w/v Cytidine 5’-diphosphocholine sodium salt dihydrate, 0.02 M HEPES sodium pH 6.8
65
(F5)
0.20% w/v Biotin, 0.20% w/v Phospho(enol)pyruvic acid monosodium salt hydrate, 0.20% w/v Sodium pyruvate, 0.20% w/v D-(+)-Melezitose hydrate,
0.20% w/v Coenzyme B12, 0.02 M HEPES sodium pH 6.8
66
(F6)
0.20% w/v Flavin adenine dinucleotide disodium salt hydrate, 0.20% w/v D-(−)-3-Phosphoglyceric acid disodium salt, 0.20% w/v Spermine,
0.20% w/v Adenosine-5’-diphosphoglucose disodium salt, 0.20% w/v L-Tyrosine, 0.02 M HEPES sodium pH 6.8
67
(F7)
0.20% w/v Thymine, 0.20% w/v Sodium pyrophosphate tetrabasic decahydrate, 0.20% w/v D-Glyceric acid calcium salt dihydrate, 0.20% w/v b-Cyclodextrin,
0.20% w/v myo-Inositol, 0.02 M HEPES sodium pH 6.8
68
(F8)
0.20% w/v b-Nicotinamide adenine dinucleotide hydrate, 0.20% w/v D-Fructose 1,6-bisphosphate trisodium salt octahydrate, 0.20% w/v Spermidine,
0.20% w/v Adenosine 5’-diphosphoribose sodium salt, 0.20% w/v Histamine dihydrochloride, 0.02 M HEPES sodium pH 6.8
69
(F9)
0.20% w/v Pyridoxal 5-phosphate monohydrate, 0.20% w/v Pyruvic acid, 0.20% w/v b-Cyclodextrin, 0.20% w/v (±)-Epinephrine,
0.20% w/v myo-Inositol 2-monophosphate bis(cyclohexylammonium) salt, 0.02 M HEPES sodium pH 6.8
70
(F10)
0.20% w/v Adenosine 5’-diphosphate sodium salt, 0.20% w/v Adenosine 5’-monophosphate sodium salt,
0.20% w/v Uridine 5’-monophosphate disodium salt hydrate, 0.20% w/v Cytidine 5’-diphosphate sodium salt hydrate,
0.20% w/v Cytidine 5’-monophosphate disodium salt, 0.02 M HEPES sodium pH 6.8
71
(F11)
0.20% w/v Pyridoxal hydrochloride, 0.20% w/v 2’-Deoxyadenosine 5’-monophosphate, 0.20% w/v Guanosine 5’-diphosphate sodium salt, 0.20% w/v Nalidixic acid,
0.20% w/v Uridine 5’-diphospho-N-acetylglucosamine sodium salt, 0.02 M HEPES sodium pH 6.8
72
(F12)
0.25% w/v Guanosine 5’-triphosphate sodium salt hydrate, 0.25% w/v Thymidine 5’-monophosphate disodium salt hydrate, 0.25% w/v L-Tryptophan,
0.25% w/v Inosine 5’-triphosphate trisodium salt, 0.02 M HEPES sodium pH 6.8
0.25% w/v N-Acetyl-D-galactosamine, 0.25% w/v Gentamicin sulfate salt hydrate, 0.02 M HEPES sodium pH 6.8
0.20% w/v 2’-Deoxycytidine 5’-monophosphate sodium salt, 0.02 M HEPES sodium pH 6.8
0.20% w/v Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate, 0.04% w/v Acetyl coenzyme A sodium salt, 0.02 M HEPES sodium pH 6.8
Scoring Sheet - HR2-088
Page 3 of 4
© 1991 - 2015 Hampton Research Corp.
silver bullets bio
TM
HR2-088 - Scoring Sheet
Sample:
Sample Concentration:
Sample Buffer:
Date:
Reservoir Volume:
Drop Volume: Total
G1 - G12
73
(G1)
74
(G2)
75
(G3)
76
(G4)
Temperature:
ml
Sample
ml
Reservoir
ml
Additive
ml
1
2
3
4
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Date
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Date
Date
0.20% w/v 2’-Deoxyguanosine hydrate, 0.02 M HEPES sodium pH 6.8
10% v/v Tacsimate pH 7.0, 0.02 M HEPES sodium pH 6.8
0.20% w/v DL-a-Lipoic acid, 0.20% w/v Creatine monohydrate, 0.20% w/v L-Glutathione reduced, 0.20% w/v D-Pantothenic acid hemicalcium salt,
0.20% w/v g-Aminobutyric acid, 0.02 M HEPES sodium pH 6.8
0.20% w/v Pyridoxal hydrochloride, 0.20% w/v Nicotinamide, 0.20% w/v Batyl Alcohol, 0.20% w/v Glutaric acid, 0.20% w/v N-Acetyl-L-glutamic acid, 0.02 M HEPES sodium pH 6.8
0.20% w/v Riboflavin 5’-monophosphate sodium salt dihydrate, 0.20% w/v Maleic acid, 0.20% w/v Pyridoxamine dihydrochloride, 0.20% w/v Acetylcholine chloride,
78
(G6)
0.20% w/v Fumaric acid, 0.20% w/v cis-Aconitic acid, 0.20% w/v DL-Isocitric acid trisodium salt, 0.20% w/v Oxalacetic acid, 0.20% w/v Sodium pyruvate,
79
(G7)
0.04% w/v b-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate, 0.04% w/v Tetrahydrofolic acid, 0.04% w/v L-Ascorbic acid,
80
(G8)
0.16% w/v Riboflavin, 0.16% w/v L-a-Phosphatidylcholine, 0.16% w/v D-(+)-Raffinose pentahydrate, 0.16% w/v D-(−)-3-Phosphoglyceric acid disodium salt,
81
(G9)
0.16% w/v 2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate, 0.16% w/v Cholesterol, 0.16% w/v Thymine, 0.16% w/v Oxamic acid,
82
(G10)
0.20% w/v Thiamine monophosphate chloride dihydrate, 0.20% w/v Creatine phosphate disodium salt tetrahydrate, 0.20% w/v Na-Benzoyl-L-arginine ethyl ester hydrochloride,
83
(G11)
0.20% w/v Uridine 5’-triphosphate trisodium salt hydrate, 0.20% w/v Kanamycin monosulfate, 0.20% w/v Maltotriose hydrate, 0.20% w/v Leupeptin hydrochloride,
84
(G12)
0.16% w/v 5-Phospho-D-ribose 1-diphosphate pentasodium salt, 0.16% w/v Maleic acid, 0.16% w/v N-Acetyl-D-glucosamine, 0.16% w/v L-(−)-Malic acid sodium salt,
85
(H1)
Posettes or Spherulites
Needles (1D Growth)
Plates (2D Growth)
Single Crystals (3D Growth < 0.2 mm)
Single Crystals (3D Growth > 0.2 mm)
0.20% w/v Adenosine 5’-triphosphate disodium salt hydrate, 0.20% w/v 2’-Deoxycytidine 5’-monophosphate sodium salt, 0.20% w/v b-Estradiol, 0.20% w/v D-(+)-Galactose,
77
(G5)
H1 - H12
5
6
7
8
9
Clear Drop
Phase Separation
Regular Granular Precipitate
Birefringent Precipitate or
Microcrystals
0.20% w/v Uridine 5’-diphospho-N-acetylglucosamine sodium salt, 0.02 M HEPES sodium pH 6.8
0.02 M HEPES sodium pH 6.8
0.04% w/v D-(+)-Glucose, 0.04% w/v Folic acid, 0.04% w/v D-Pantothenic acid hemicalcium salt, 0.02 M HEPES sodium pH 6.8
0.16% w/v g-Aminobutyric acid, 0.16% w/v Thionicotinamide adenine dinucleotide, 0.02 M HEPES sodium pH 6.8
0.16% w/v D-Glucosamine 6-phosphate sodium salt, 0.16% w/v Guanosine 3’,5’-cyclic monophosphate sodium salt, 0.02 M HEPES sodium pH 6.8
0.20% w/v Tetracycline hydrochloride, 0.20% w/v Succinic acid disodium salt hexahydrate, 0.02 M HEPES sodium pH 6.8
0.20% w/v Sodium glyoxylate monohydrate, 0.02 M HEPES sodium pH 6.8
0.16% w/v g-Aminobutyric acid, 0.16% w/v Acetylcholine chloride, 0.02 M HEPES sodium pH 6.8
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Date
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Date
0.05% w/v Glycine, 0.05% w/v L-(-)-Threonine, 0.05% w/v L-(+)-Lysine, 0.05% w/v L-Alanine, 0.05% w/v L-Arginine, 0.05% w/v L-Asparagine monohydrate,
0.05% w/v L-Aspartic acid, 0.05% w/v L-Glutamic acid, 0.05% w/v L-Glutamine, 0.05% w/v L-Histidine, 0.05% w/v L-Isoleucine, 0.05% w/v L-Leucine, 0.05% w/v L-Methionine,
0.05% w/v L-Phenylalanine, 0.05% w/v L-Proline, 0.05% w/v L-Serine, 0.05% w/v L-Tryptophan, 0.05% w/v L-Tyrosine, 0.05% w/v L-Valine, 0.02 M HEPES sodium pH 6.8
86
(H2)
0.14% w/v Gly-Gly-Gly-Gly-Gly, 0.14% w/v Leu-Gly-Gly, 0.14% w/v Ala-Ala-Ala, 0.14% w/v Met-Ala-Ser, 0.14% w/v Val-Ser, 0.14% w/v Ala-Leu, 0.14% w/v Z-Val-Phe,
87
(H3)
0.16% w/v Adenosine 5’-triphosphate disodium salt hydrate, 0.16% w/v Pyridoxal 5-phosphate monohydrate, 0.16% w/v Creatine monohydrate, 0.16% w/v Thymine,
88
(H4)
0.16% w/v Adenosine 5’-triphosphate disodium salt hydrate, 0.16% w/v Riboflavin 5’-monophosphate sodium salt dihydrate, 0.16% w/v DL-a-Lipoic acid,
89
(H5)
0.16% w/v Creatine phosphate disodium salt tetrahydrate, 0.16% w/v b-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate, 0.16% w/v Guanosine 5’-triphosphate
90
(H6)
0.14% w/v Thiamine monophosphate chloride dihydrate, 0.14% w/v b-Nicotinamide adenine dinucleotide hydrate, 0.14% w/v Guanosine, 0.14% w/v Thymidine,
91
(H7)
0.16% w/v 2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate, 0.16% w/v Thymidine 5’-triphosphate sodium salt, 0.16% w/v 4-Aminobenzoic acid, 0.16% w/v Cytosine,
92
(H8)
0.16% w/v b-Nicotinamide adenine dinucleotide, reduced dipotassium salt, 0.16% w/v Guanidine hydrochloride, 0.16% w/v Cystamine dihydrochloride, 0.16% w/v Aspartame,
93
(H9)
0.20% w/v Uridine 5’-triphosphate trisodium salt hydrate, 0.20% w/v Adenosine 5’-monophosphate sodium salt, 0.20% w/v N-Acetylneuraminic acid, 0.20% w/v L-Tyrosine,
94
(H10)
0.16% w/v Pyridoxamine dihydrochloride, 0.16% w/v Guanosine 5’-diphosphate sodium salt, 0.16% w/v Cytidine 5’-triphosphate disodium salt,
95
(H11)
0.16% w/v L-Carnitine hydrochloride, 0.16% w/v b-Nicotinamide adenine dinucleotide phosphate tetrasodium salt, 0.16% w/v 2’-Deoxyadenosine 5’-triphosphate disodium salt,
96
(H12)
0.16% w/v Uridine 5’-monophosphate disodium salt hydrate, 0.16% w/v D-(+)-Maltose monohydrate, 0.16% w/v D-Sorbitol, 0.16% w/v O-Phospho-L-serine,
0.02 M HEPES sodium pH 6.8
0.16% w/v L-(−)-Malic acid sodium salt, 0.16% w/v Spermine, 0.02 M HEPES sodium pH 6.8
0.16% w/v Acetylsalicylic acid, 0.16% w/v L-Ornithine hydrochloride, 0.16% w/v Adenosine, 0.02 M HEPES sodium pH 6.8
sodium salt hydrate, 0.16% w/v Uridine, 0.16% w/v D-Pantothenic acid hemicalcium salt, 0.16% w/v N-Acetyl-D-glucosamine, 0.02 M HEPES sodium pH 6.8
0.14% w/v D-(+)-Galactose, 0.14% w/v Biotin, 0.14% w/v D-Fructose 1,6-bisphosphate trisodium salt octahydrate, 0.02 M HEPES sodium pH 6.8
0.16% w/v Benzamidine hydrochloride, 0.16% w/v b-Nicotinamide adenine dinucleotide, reduced dipotassium salt, 0.02 M HEPES sodium pH 6.8
0.16% w/v Caffeine, 0.16% w/v Cytidine 5’-monophosphate disodium salt, 0.02 M HEPES sodium pH 6.8
0.20% w/v L-Thyroxine, 0.02 M HEPES sodium pH 6.8
0.16% w/v D-Glucose 6-phosphate sodium salt, 0.16% w/v O-Phospho-L-tyrosine, 0.16% w/v Spermidine, 0.02 M HEPES sodium pH 6.8
0.16% w/v Choline base solution, 0.16% w/v a-D-Glucose 1-phosphate disodium salt hydrate, 0.16% w/v Riboflavin, 0.02 M HEPES sodium pH 6.8
0.16% w/v Benzidine, 0.16% w/v Sodium phenyl phosphate dibasic dihydrate, 0.02 M HEPES sodium pH 6.8
Scoring Sheet - HR2-088
Page 4 of 4
© 1991 - 2015 Hampton Research Corp.