Cattle production Part 1: Growing food for cattle Introduction ............................................................................... 2 Photosynthesis .......................................................................... 3 The rate of photosynthesis ...............................................................4 Questions .........................................................................................7 Respiration ................................................................................ 9 What is respiration?..........................................................................9 Photosynthesis and respiration ......................................................10 Net assimilation rate.......................................................................11 Experimental design................................................................ 13 Experimental design factors ...........................................................13 Analysis and evaluation of experiments.........................................18 Designing and conducting an experiment ......................................20 Pastures .................................................................................. 30 Introduced pasture .........................................................................30 Native pasture ................................................................................31 Pasture management .....................................................................32 Summary................................................................................. 33 Additional resources................................................................ 34 Suggested answers................................................................. 38 Exercises – Part 1 ................................................................... 46 Part 1: Growing food for cattle 1 Introduction Introduction A cattle production system requires cattle food as an input. The amount of food and its quality will influence how well the cattle will survive and produce. In the majority of Australian cattle production systems the plants and animals interact in a grazing system, each one affecting the other. This part will begin by examining how a plant utilises solar energy to produce sugars via the process of photosynthesis. The process of respiration and the resulting net assimilation rate will also be investigated. These processes can be manipulated by management practices to maximise agricultural production. The principles of experimental design are studied and used to design an experiment on planting density in grazing oats. This experiment will take you seven weeks to complete. The use of native and introduced pastures in grazing systems is then investigated. This part contributes towards an understanding of Outcome H2.1 from the Agriculture Stage 6 HSC Course. The syllabus can be found on the Board of Studies, NSW website at http://www.boardofstudies.nsw.edu.au 2 Cattle production Photosynthesis Photosynthesis Access an interactive version of Photosynthesis and Respiration using this link. Resume with this material on page 14: Experimental design. Plants obtain energy to grow and develop from the sun. This occurs through a process called photosynthesis. photo = light synthesis = manufacture The products of photosynthesis provide the basis for plant growth by providing the chemical building blocks. These building blocks, with other nutrients absorbed by the roots, allow plants to make all the compounds needed for their growth and development. Plants then provide food for humans and other animals either directly or indirectly. The overall chemical equation for photosynthesis can be summarised as follows: sunlight 6CO2 + carbon + dioxide 6H 2 O water chlorophyll sunlight chlorophyll C 6 H 12 O 6 + 6O2 simple sugars + oxygen Photosynthesis is actually a series of reactions in which chlorophyll traps light energy to split water molecules into hydrogen and oxygen. The oxygen is released from the plant. Energy rich compounds formed during the splitting of the water molecules are used to combine the hydrogen with carbon dioxide to form simple sugars that are the final products of photosynthesis. Part 1: Growing food for cattle 3 You should revise Part 1 from Down to earth in your preliminary course notes on the various parts of plants and their morphology to help you to understand this work. Cell organelles called chloroplasts are made up of layers composed of proteins, lipids (fats) and a green pigment called chlorophyll. Chlorophyll absorbs the light energy that drives the light reactions of plants, so photosynthesis can occur wherever plants are green – mainly in the leaf, but also in the stems and shoots. Water reaches sites of photosynthesis via the xylem after entering the plant from the soil through the roots. Carbon dioxide, the other raw material for photosynthesis, enters the leaf tissue through the stomata from the atmosphere. Oxygen, released from the cells during photosynthesis, escapes through the stomata. Photosynthesis produces simple sugars such as glucose. Glucose is converted into starch for temporary storage in the leaf. Because starch is insoluble and too large to be moved to other parts of the plant it is broken down to sucrose before it is moved. The movement of sugars around the plant is called translocation. Figure 1.1: Translocation in plants. The rate of photosynthesis The rate of photosynthesis in a plant is affected by environmental factors such as light, availability of water, temperature, carbon dioxide concentration, availability of mineral nutrients and by the genetic potential of the plant. These factors can also be termed constraints or limiting factors. Any of these factors can limit the rate of photosynthesis. If the rate of photosynthesis is increased, what will happen to the rate of growth and development? 4 Cattle production _________________________________________________________ Check your answer. Light The diagram below shows that as the intensity of light increases the rate of photosynthesis increases, until a point is reached where there is no further increase. From then on the rate remains constant, regardless of increasing light intensity. rate of photosynthesis Tropical plant species Temperate plant species shade light intensity Figure 1.2: Light intensity and photosynthesis. Different plants have different light requirements, and their maximum rates of photosynthesis occur at different light intensities. Ferns tend to grow best when they are in shady situations because they are suited to lower light intensities, while some other plants will do well only in full sunlight. In some horticultural industries, shadecloth and other materials can be used to create the light intensities preferred by the plants being grown. Water Water is one of the important inputs of photosynthesis, so a shortage of water will result in a decreased photosynthetic rate. Complete lack of water will result in the death of the plant. Irrigation is a common practice in farming to supplement natural rainfall. This ensures that lack of water will not limit photosynthesis and plant production. Part 1: Growing food for cattle 5 Temperature The effect of temperature on the rate of photosynthesis is shown below. Photosynthesis reaches a maximum then quickly declines at higher temperatures in temperate species such as wheat and soybean. Tropical species tend to reach their maximum at higher temperatures. relative photosynthesis tropical species temperate species temp Figure 1.3: Temperature and photosynthesis. Carbon dioxide rate of photosynthesis As the concentration of carbon dioxide in the air surrounding a plant increases, the rate of photosynthesis also increases, as shown in the graph below. CO2 conc Figure 1.4: Carbon dioxide and photosynthesis. The normal concentration of carbon dioxide in the air is 0.03%. Carbon dioxide concentrations above this increase the rate of photosynthesis. In some glasshouses, carbon dioxide can be added to the air to achieve greater growth rates. 6 Cattle production Mineral nutrients Phosphorus is involved in the capture and transfer of energy in the photosynthetic process, and magnesium is an important part of the chlorophyll molecule that initially traps the light energy. Deficiency of these mineral nutrients will therefore result in decreased photosynthesis and growth. Fertilisers are commonly used to ensure that lack of mineral nutrients will not limit growth and therefore plant production. Genetic potential Species vary in their ability to carry out photosynthesis. Each species is best adapted to the environment where they developed. Wheat is suited to temperate climatic conditions; sugar cane is suited to the warmer tropics. Differences in photosynthetic capacity also occur between varieties within a species. For example, certain varieties of soybean show an increased photosynthetic rate of up to 20% under the same environmental conditions. Questions 1 Answer the following true/false statements about photosynthesis. Code each of the statements T if you think they are TRUE and F if you think they are FALSE. a Sunlight is essential for the photosynthesis reaction. b Photosynthesis takes place in all parts of the plant. c Sugars and starch are carbohydrates. d Water is taken into the plant through the leaves. e Carbon dioxide is taken into the plant through the stomata. f As the intensity of light increases the rate of photosynthesis will continue to increase indefinitely. g Tropical plants have a greater potential for maximum growth than temperate plants. h Water availability has no effect on photosynthetic rate. Part 1: Growing food for cattle 7 2 Rewrite each of the false statements so they are correct. ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ 3 A farmer growing flowers in glasshouses achieves higher temperatures inside the glasshouse than outside. This is due to the solar radiation trapped in the glasshouse during the day. Carbon dioxide is also added to the air during the day to increase the photosynthetic rate of the crop. The farmer is aiming to increase the rate of photosynthesis of the flower crop and hence increase the yield. To ensure that this occurs, the farmer needs to reduce the effects of other limiting factors. Identify two factors that could limit the photosynthetic rate of the crop and describe how each one would have an effect on the final growth of the crop. (Assume that temperature and carbon dioxide levels would not be limiting.) ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ 4 In some crops, for example tomatoes, pruning is carried out to reduce the leaf cover and thus the effect of shading. Explain why a farmer would want to prune back some of the leaf cover. What effect would pruning have on a crop where the marketable product is not the leaves? ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ Check your answers. 8 Cattle production Respiration Respiration Now you will find out what happens to the sugars produced by photosynthesis. Some of the sugars produced by photosynthesis are used by the plant for growth and development. This is important in agriculture as it is how the plant makes the products that we will harvest, then sell for income. Some of the sugars are used for other processes within the plant. One of these processes is respiration. What is respiration? Respiration is a process that occurs in all living cells. Simple sugars, are broken down into carbon dioxide, water and energy. The energy is used by cells to carry out their metabolic processes. C 6H 12 O 6 simple sugars + 6O 2 + oxygen 6CO 2 + 6H 2 O + carbon + water dioxide + energy energy At a superficial level respiration appears to be the reverse of photosynthesis. There are some important differences between respiration and photosynthesis. • Respiration occurs continuously (24 hours a day). Photosynthesis occurs only when there is sufficient light. • Respiration occurs in all cells, whereas photosynthesis occurs only in plant cells where there is chlorophyll. Part 1: Growing food for cattle 9 Photosynthesis and respiration Code each of the following statements. Write P if you think the statement refers to photosynthesis and R if you think the statement refers to respiration. ______ 1 Sunlight is essential for this process to occur. 2 This process occurs 24 hours a day. 3 The process that produces a continuous supply of available energy. 4 Carbon dioxide is needed for this process. 5 This process occurs only in the green parts of plants. 6. This process occurs in all living cells. ______ ______ ______ ______ ______ Check your answers. Rates of photosynthesis and respiration Sugars are produced by photosynthesis. These sugars are used in respiration. For a plant to grow there must be sugars remaining after use by respiration. That is, the rate of photosynthesis must be greater than the rate of respiration. ratio of sugar production (photosynthesis) or consumption (respiration) Study the graph below and answer the questions that follow. photosynthesis respiration MT 6 noon 6 MT time Figure 1.5: Rate of sugar production and consumption. 1 10 Identify the period of the day when photosynthesis takes place. Cattle production _____________________________________________________ 2 Identify the period of the day when respiration takes place. _____________________________________________________ 3 Identify the period of the day when the rate of photosynthesis exceeds the rate of respiration. _____________________________________________________ 4 Explain the implications of this period (when the rate of photosynthesis exceeds the rate of respiration) for the plant. _____________________________________________________ _____________________________________________________ _____________________________________________________ Check your answers. Net assimilation rate Net assimilation rate (NAR) is a measure of the growth of plants resulting from the difference between photosynthesis and respiration. It gives an important indication of the growth of the crop. NAR is an estimate of the average rate of dry matter gain per unit area of leaf in a given time. The interaction between photosynthesis and respiration is a crucial relationship that determines the rate at which plants grow. What factors affect NAR? They include: • rate of photosynthesis • rate of respiration – respiration rate is influenced by temperature and the availability of oxygen and sugars within the plant • temperature – high temperatures during the day and low temperatures during the night give a higher NAR because high day temperatures increase the rate of photosynthesis and low night temperatures decrease the rate of respiration • nutrient availability such as nitrogen, phosphorus, sulfur and potassium • leaf area – in general the greater the leaf area the more production of food Part 1: Growing food for cattle 11 • proportion of non photosynthetic material – the more roots, stems, flowers and fruits there are in the plant, the more respiration will take place compared to photosynthesis • photoperiod –daylength affects the amount of photosynthesis carried out by a plant • stage of plant development – at different stages the rates of photosynthesis and respiration will vary • canopy structure – the arrangement of the leaves on a plant can influence the photosynthetic rate especially if there is excessive shading of the leaves. Outline three management practices that could be used by a farmer to maximise the NAR of the plants being grown. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Check your answers. 12 Cattle production Experimental design Experimental design Access an interactive version of Experimental design using this link. Resume with this material on page 21: Designing and conducting an experiment. Experiments are carried out to test predictions. The purpose of an experiment is to provide evidence to support a prediction or hypothesis. Sometimes poor experimental design can lead to results that are inaccurate or invalid. Even experiments carried out by professionals can be poorly designed. The better the design, the more reliable the results will be. In the preliminary course (Hands on) you examined how to identify and use treatment and control in an experimental design. Read Part 2 again to remind yourself of the terms used and what they mean. In this part you will investigate other important aspects of experimental design that you will have to consider whenever you encounter experiments. Experimental design factors You should develop your ability to critically analyse the method used in an experiment. When analysing a design the following aspects need to be considered: • control • randomisation • replication • standardisation • data collection. You should assess whether each of these areas have been well accommodated in the design or not. Randomisation This is where you use a random method of allocating experimental subjects to a treatment or treatments. A random method could be tossing a coin, rolling a dice, drawing numbers or tokens out of a hat, use of Part 1: Growing food for cattle 13 random number tables or electronically generated random numbers. The method chosen depends on the one most suited to the experiment. Use of a random method avoids bias in the experiment, which could alter the results. Randomisation is important in experiments so that unexpected variations can be accommodated without changing the outcome. Figure 1.6: Avoid bias by generating random numbers. 1 A researcher sets up an experiment taking seeds from a bag one at a time and placing each seed in a pot. They begin with the pots that will have treatment A and then continue with those for treatment B. The seeds have settled in the bag and the larger seeds tend to be towards the bottom of the bag. a) Outline how the allocation of seed to pots could result in bias in this experiment. __________________________________________________ __________________________________________________ b) Outline a random method of allocating seeds to pots that would avoid bias. Check that your outline mentions: • the random method used, for example, tossing a coin • how the random method is used for allocation • how the method avoids bias. __________________________________________________ __________________________________________________ __________________________________________________ __________________________________________________ 2 14 The researcher randomly allocates seeds, pots and treatments and then lines up the treatment A pots on one side of the greenhouse, and Cattle production the treatment B pots on the other side. It is known that the watering system in the greenhouse is uneven, and one treatment will get more water than the other. a) Explain why this arrangement of pots could result in a biased experiment. _________________________________________________ _________________________________________________ b) Outline a random method of pot arrangement to avoid bias in the greenhouse. _________________________________________________ _________________________________________________ _________________________________________________ Check your answers Replication Replication is also important in making the result more reliable. Figure 1.7: Is the difference due to the treatment or the uneven watering? Review the situation outlined in question 2 on the previous page. Imagine if the researcher had only one pot of each treatment. The level of error in the experiment (caused by the faulty watering system) could not be distinguished from the effect of the treatment itself. This situation would be likely to result in incorrect experimental conclusions. Having a number of pots with the same treatment means that slight variations are averaged out and the results are more accurate. Part 1: Growing food for cattle 15 How many replicates are needed? The more replications there are the more reliable the results of the experiment become. However, every experiment has restricted resources, for example, land area, labour or money. Good experiments will include as many replicates as possible given the restrictions. The more replicates, the more confident you can be of accurate results. Whenever you are evaluating an experimental design you should consider whether there are sufficient replicates. If there are few replicates and the treatment results are not very different, then the experiment may show an inaccurate result. The researcher should be cautious in making recommendations. Animal experiments, especially those dealing with larger animals are likely to have fewer replicates than plant experiments. Standardisation An experiment will have a variable that is being tested. If all conditions except the variable are the same then any difference in the results must be due to the variable. When evaluating an experimental design consider how well particular aspects of the experiment have been standardised. You should mention aspects that are kept the same as well as those that might be different. List aspects that could be standardised in an experiment involving a field crop. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Check your answer. The more standard the conditions the more reliable the results of the experiment. Data collection All experiments need to have a way of evaluating the effect of the variable. This must be in the form of a measurement. In agricultural 16 Cattle production experiments it is common to compare the amount of the agriculturally important product. For example an experiment using cabbages would compare the weight of the cabbage heads for each of the treatments to decide which was the better treatment. Figure 1.8: The cabbage head is the agriculturally important part of the crop Complete the following table to show what part of the plant or animal you could measure and the units you could use. Plant or Animal Part measured Units cabbages cabbage head kilograms/ ha layer chickens wheat beef cattle Check your answer. Decisions about measurement The method of making measurements should be decided before the experiment begins. If an experimenter needs to measure the leafy parts of a plant, then measuring the length and number of leaves will give you limited information about the quantity of leaf. A better indicator is to weigh the leaf parts. However, this will destroy the plant so it may be unsuited to ongoing experiments. A problem with weighing is that a large proportion of a leaf will generally consist of water. The amount of water will vary according to the environmental conditions at the time it was cut, so for better accuracy Part 1: Growing food for cattle 17 the leaf is usually dried in an oven so that the dry matter weight can be established. This measurement is also useful when comparing nutritional values of different animal feeds. Analysis and evaluation of experiments Analyse is one of the key words used in the Board of Studies syllabuses and examinations. It has a particular meaning when used in HSC exam questions. Analyse – identify components and BOS NSW 1999 The New Higher School Certificate Assessment the relationship between them; support Document draw out and relate implications To analyse an experimental design you need to: • identify the design aspects – control – randomisation – replication – standardisation – data collection • provide details of how well each design aspect is addressed • outline the implications of the good and bad points of the design. • indicate how the design could be improved. Now you will analyse the design of the wheat variety experiment that follows. Wheat variety experiment Two adjoining fields of the same size are selected for a trial. These fields have similar cropping histories. Nutrient and pH tests show similar results for each. Soil texture and structure are the same. The fields are cultivated at the same time, with the same machinery. A combine seeder is used to plant wheat seed and add superphosphate fertiliser at the same rate for each field. One field is planted with the previously used Cunningham variety and the other field with the new Sunco variety. 18 Cattle production Figure 1.9: Wheat crop. When spraying for the weed ryegrass is required, the same herbicide is applied with a boomspray to both fields at the same application rate. When the grain has ripened the fields are harvested separately, and the resulting grain from each field is weighed. Analyse the experimental design. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Part 1: Growing food for cattle 19 _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Check your answer. Designing and conducting an experiment You will be using all the aspects of experimental design you have learnt to design and conduct an experiment on the effect of planting density on grazing oats. Oats are a very flexible crop that can be used for grain, hay and forage (green feed). They are widely used in the southern states of Australia for winter forage production as they have higher daily winter growth rates than other cereals and most pastures. Aim The aim of the experiment will be to determine the optimum planting density for maximum dry matter production of grazing oats. Planting density When an oat plant first emerges it has a single leaf. It will grow more leaves until there are about four. At this stage the plant develops tillers. A tiller will grow from the plant stem near ground level; it looks a bit like another plant growing very close. The more space that the plant has around it, the more tillers it is likely to develop. A crowded plant might not develop tillers at all, and instead, grow taller to better compete for sunlight. Look at the following diagram showing the growth stages of cereal crops. 20 Cattle production Figure 1.10: Growth stages of cereal crops (Adapted from NSW Agriculture Winter Crop Variety Sowing Guide 2002). Usually about 6–8 weeks after emergence grazing oats have livestock put onto them for their first grazing. When the plants have been eaten down to about 10 cm above soil level, the livestock are removed and the plants are allowed to recover. Depending on the growing conditions, the farmer might get two or three grazing periods from the oats. After grazing, dual purpose varieties are locked away from livestock and grain is allowed to develop for harvest. If plants are very crowded they will not have enough resources available to produce well developed reproductive parts. yield reproductive) The following graph illustrates how reproductive yield changes as plant density increases for a given area. Notice that as the density increases, so does yield until the plants become too crowded and yield starts to decline. density Figure 1.11: Reproductive yield and density (Based on Lovett et al, 1988, p 47) Part 1: Growing food for cattle 21 In this experiment we are interested in maximising the amount of dry matter produced for the first grazing in a given area. Smaller numbers of plants do not necessarily mean less production because more tillers will be produced, but at a very low density the tillers will not fill the space, and production for the area will decrease. At increasing densities production should remain high, although the size of individual plants will decrease. yield (vegetative) Sketch in the space below the shape of the vegetative production curve you would expect as density is increased. density Figure 1.12: Vegetative yield and density. Check your answer. Experimental design In this experiment you will plant oat seeds at different densities in a series of large pots, and grow them for seven weeks. Then you will harvest the oat leaves and stems by cutting them 10 cm above soil level. The leaves and stems from each pot will be placed in a numbered paper bag, dried, then weighed to obtain a measurement of the dry matter produced by each pot. Before you begin you will need to develop your experimental design. 1 Identify the variable that you will be testing in this experiment. _____________________________________________________ 2 Explain how the design aspect of a control is included in this experiment. ______________________________________________________ ______________________________________________________ ______________________________________________________ 3 22 Explain how randomisation can be used in the experiment to avoid bias. Cattle production _____________________________________________________ _____________________________________________________ _____________________________________________________ _____________________________________________________ Consider the reasonable limits of the resources you have available for the experiment. In this sort of experiment you will need more than two treatment levels so you can better find the optimum planting density. Each treatment will need to be replicated a number of times. Due to resource limits there will need to be a compromise between pinpointing the best planting density and the accuracy of the result. 4 Identify what you would consider to be a reasonable number of treatment levels and replicates for this experiment. _____________________________________________________ _____________________________________________________ Check your answers. Now you will need to decide the density of seeds in each treatment. It is recommended that the lowest density should be a single plant in a pot (the spacing is then the distance to the next pot). Normal spacing of oats in the field varies with the conditions, but generally it is approximately 10–20 cm between each plant. If you are considering four treatment levels you could have one that is very close, perhaps 2 or 3 cm spacing, and then the other two treatments at intermediate levels to give a range of spacings. Part 1: Growing food for cattle 23 Resources You will need to assemble the following resources for the experiment. Immediately: In seven weeks time: • a number of large pots • shears or scissors • potting mix sufficient to fill the pots • ruler • paper bags • fertiliser and standard scoop or scales to ensure the same amount is added to each pot • marking pen to label bags • microwave oven plant labels and waterproof marker pen • microwave safe cup • water • oat seed • • ruler scale (as accurate as possible) • measuring jug • paper and pen for recording • water. • clock for timing. • Planting In order to standardise the experiment it is important that everything, apart from the planting density, is kept as similar as possible. 1 Fill each pot with potting mix to 2–3 cm from the top of the pot. Take care when handling potting mix as some microorganisms that thrive in the nutrient rich potting mix can make you ill if you inhale or accidentally eat them. Read the label on the potting mix bag and follow the safety instructions. Wash your hands properly after handling the potting mix. 24 Cattle production 2 Fertiliser should be added in measured quantities to each pot. Farmers generally provide phosphorus and nitrogen fertilisers when growing grazing oats. If you have access to fertilisers such as superphosphate and Nitram® these can be used, otherwise check the label of the fertiliser you have available and ensure it contains these elements. Incorporate the fertiliser evenly within the top 10 cm of the pot so all the plants will have the same access. 3 Label the pots with numbers. Use a random method to allocate them to each treatment. Add the treatment to the label so you know which pot belongs to which density level. 4 Plant seed in each pot in the numerical order of the pots. Seed should be planted at a depth of about 2.5 cm. Extra seeds can be planted in each pot in case some do not sprout. Excess plants should be thinned as soon as they emerge. Use a ruler to position the seeds at the correct density for each treatment. 5 Try to find a position for the pots that does not have varying environmental conditions, for example, partial shade. If you group the pots closely, rather than have them spread out in a long line, the environmental conditions are likely to be more similar. Situate each pot in your space using a random method. Do not group the same treatment together. Figure 1.13: Partial shade in the experiment area could alter the results. Part 1: Growing food for cattle 25 6 Measure the same amount of water and apply slowly to each pot. Water the pots well to start germination. Check the pots regularly to see if they are drying out and give all the pots a measured amount of water. Harvest After about seven weeks the oats should be at the growth stage where they are ready for grazing. You will simulate this by using scissors or shears to cut the oats. Cut the oats one pot at a time. 26 1 Cut the oats 10 cm above the soil surface. Use a ruler to check the height. 2 Cut the oats into pieces of approximately 1 cm length. 3 Identify the treatment by writing it on a paper bag. Collect all the cut material and place it into the bag. 4 Use the following procedure if you have a microwave oven: • put the paper bag with the cut oats and a microwave safe cup 3/4 full of water into the microwave oven • microwave on high for 4 minutes • let the bag stand for 4 minutes • weigh the bag and record the weight • refill the cup so it remains 3/4 full and microwave the cup and bag for 1 minute • let the bag stand for 1 minute and then weigh • record the weight • continue to microwave for 1 minute and then let stand for 1 minute until the weight does not change • subtract the weight of the paper bag to find the weight of dry matter produced for that pot. Record this weight along with the identifying information from the bag. Cattle production If you do not have a microwave you may have access to a science laboratory oven at school. If you leave the oats at 105oC for 3 days (over the weekend would be good) they will reach a point called oven dry, after which they will no longer be able to lose moisture and you can then weigh them directly from the oven. If you have no other option you could use a conventional oven. Place all the bags onto a tray in an oven set at about 105oC. It may be difficult to set some domestic ovens at this low temperature, so you will need to supervise the drying as higher temperatures could present a fire hazard. Dry the material for as long as you can manage. Due to the shorter drying time the material will not be truly oven dry, but as long as all the bags are dried for the same time period, the results should be comparable. Take the bags directly from the oven and weigh them. Do not forget to subtract the weight of the bag from your measurement. Presentation When you have weighed the oats you will have a series of measurements called raw data. This raw data needs to be altered to make it more meaningful. Sometimes the raw data is processed into more meaningful numbers, for example, by calculating a mean or standard deviation for each treatment. One way that the data can be presented is in a table. A table usually groups the data into columns or rows. Another common method of presentation is as a graph. A good table or graph will allow the reader to quickly see the trend in the important data. When you look at tables and graphs try to be critical of their arrangement and value. Poor presentation of data includes tables with no sorting and multiple graphs that have no purpose. Part 1: Growing food for cattle 27 Have a look at the examples of data presentation in the Additional resources section. Each one presents the same set of data, from a germination experiment, in a different way. Look for examples that show the data more clearly. Write down reasons why the presentation of some examples is good and others are poor. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Check your answers. In many experiments there is such a lot of raw data collected that the experimenter may include only processed data, and may put raw data in an appendix or not in the report at all. You will need to present your data from the oats experiment. • Construct a table that presents the raw data, and a mean and standard deviation for each treatment. • Construct one graph to show as clearly as possible the most important information. Interpretation The next stage is to draw conclusions from your data. A professional researcher has several options, they could: • give their data to a statistician • use a statistical program on a computer • manipulate the raw data into a complex statistical formula or test that suits the experimental design • examine simple statistical calculations such as mean and standard deviation. In the core of the HSC course we will only need to use the simpler option of comparing means and standard deviations. If you do not know how to perform these calculations, this is looked at in Part 4 of Grape production. 28 Cattle production Be critical of the interpretation of results in experiments that you see. Sometimes there is such a significant difference between treatments that the interpretation of the results is easy. More often it is difficult to tell whether there is a difference between the treatments or not. Sometimes even professional researchers are so anxious to find a difference between treatments that they will claim one even though the data does not really support it. Is the new treatment better? The mean is a bit higher, so I will say it is! How do I decide? But the standard deviation is so big... Figure 1.14: Decisions. Write up the first two parts of the Exercise 1.1 report. You will need to complete and send this report to your teacher after you have finished drying and weighing the oats. Post experiment Do not discard your oat plants immediately after you have completed the experiment. Continue to care for them. Observe how the plants grow and recover after your simulated grazing. Grasses are adapted to resist damage by grazing animals. The growing points of grasses are close to or slightly under the ground, so that they are not affected by removal of the upper parts of the plant. Part 1: Growing food for cattle 29 Pastures Pastures The mixture of different plant species in a grazing system is called a pasture. A pasture is usually a mixture of legumes and grasses, each providing the animal with different nutrients. A legume, in association with the correct symbiotic bacteria, is able to fix nitrogen from the atmosphere and incorporate it into the plant matter. This is looked at in Part 1 of Grape production. Nitrogen is a major component of protein, so the high level of nitrogen means that legumes are an important source of protein for the grazing animal. Grasses are very high in carbohydrates. Some of the carbohydrate is starch, but the structural part of grass is mostly cellulose. The cellulose provides important roughage in the diet of a ruminant animal. Revise this from Part 3 of Looking at ewe. Complete the following sentences. 1 Carbohydrates provide animals with __________________ 2 Protein is needed by animals for _____________ and _________________ Check your answers. Pastures can range from plant communities that consist of native plant species to pastures consisting of introduced species. Introduced pasture The use of European agricultural methods in Australia has meant that in many areas, particularly the southern temperate regions, the existing native pasture has been removed and replaced with high producing species from other countries. This is called pasture improvement. For best performance these species usually require relatively high levels of inputs like fertiliser and water (irrigation). These pastures also require a high level of management to perform well. 30 Cattle production Species selection is based on the compatability of the species, their suitability to the local climate and soil, and the yearly feed requirements. It is usual to select a mixture of grasses and legumes. Legumes build up the levels of soil nitrogen and grass will use this nitrogen to grow faster. Tall grasses are not only less digestible for livestock, but will shade legumes, reducing their growth. Grasses keep out weeds and reduce the risk of bloat caused by legumes. A good manager will try to maintain a balance of legume and grass in the pasture. Management techniques that can be used to manipulate the pasture balance include: • timing and intensity of grazing • cutting for hay or slashing tall pasture • addition of fertiliser • control of pests or diseases • resowing pasture. Native pasture In many parts of Australia there has been little or no pasture improvement. Large expanses of grazing land in the northern parts of Australia have climatic and soil conditions that make the land unsuitable for intensive agricultural production. Cattle are kept here at a very low stocking rate, as higher rates would result in removal of vegetation and erosion. This type of agriculture involves very few inputs, and is well suited to the use of native pasture species. For many years the management and qualities of native pastures were generally ignored, but in recent years there has been increased interest in the use of native pastures, and a number of institutions are currently undertaking research. Native species are well adapted to the specific areas where each is found. This high level of local adaptation means that they will continue to live (persist) in very adverse conditions, like areas with low rainfall and extreme heat or fire, and they do not require high input levels. Unfortunately native pastures are usually less digestible, less palatable (tasty) and slower growing. Researchers have also had some trouble collecting, cleaning and sowing native pasture seed. 1 Outline the advantages of native pasture. _____________________________________________________ _____________________________________________________ _____________________________________________________ Part 1: Growing food for cattle 31 2 Outline the advantages of introduced pasture species ______________________________________________________ ______________________________________________________ ______________________________________________________ Check your answers. Despite the difficulties, some native species have been domesticated, and plant breeders have developed cultivars that are suited to various conditions. A good example of a domesticated native pasture species is wallaby grass. Answer the questions in Exercise 1.2 using the Agfact P2.5.39 Wallaby grass – a domesticated native grass. This agfact can be purchased from NSW DPI or downloaded free from their website at http://www.agric.nsw.gov.au Pasture management The usual objective of a pasture manager is to grow pasture that can sustainably maintain maximum animal production. Sustainability, as you will remember, is the development of a balance between profit, and the maintenance of a healthy environment that will continue to produce in future generations. Use information about pastures in this part, and from Part 2 of Crop this to help you answer the following question. Outline sustainable management practices that can be used in a pasture production system. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Check your answer. 32 Cattle production Summary Summary • Photosynthesis is a process that occurs in the green parts of plants. • The chemical equation for the photosynthesis reaction can be summarised as: 6CO 2 + • 6H 2 O sunlight chlorophyll C 6H 12 O 6 + 6O 2 Factors that affect the rate of photosynthesis include: – temperature – light – availability of water – carbon dioxide concentration – availability of mineral nutrients – genetic potential of the plant. • Respiration is a process that occurs in all living cells, 24 hours a day. • The reaction of respiration can be summarized as: C 6H 12 O 6 + 6O2 6CO 2 + 6H 2 O + energy • NAR is a measure of plant growth resulting from the difference between photosynthesis and respiration. For a plant to grow, the rate of production of material by photosynthesis must exceed the rate at which this material is used in respiration. • Good experimental design incorporates the elements of: – control – randomisation – replication – standardisation. • Planting density affects production levels in a predictable curve. • Pastures are usually a mixture of grasses and legumes. • Improved pastures are high producing, digestible and palatable, but have high levels of management and inputs. • Native pastures are locally adapted and persistent, with low input levels. Part 1: Growing food for cattle 33 Additional resources Additional resources Results of seed germination trial Seeds were placed in nine saucers and randomly allocated to three areas with different temperatures; a bench, a fridge and a warm cupboard. The number of seeds that germinated in each saucer was counted. The results follow. Saucer 1 Saucer 2 Saucer 3 Bench 23 17 12 Fridge 5 1 2 15 24 17 Warm cupboard Total Germinated 116 Example 1 Bench Fridge Warm cupboard 23 5 15 17 1 24 12 2 17 Total 52 8 56 Mean 17.3 2.7 18.7 St Dev 5.5 2.1 4.7 Example 2 34 Cattle production 30 Bench 25 Fridge Warm cupboard 20 15 10 5 0 1 2 3 Saucer Number Example 3 200 Bench Fridge 150 Warm cupboard 100 50 0 1 2 3 Saucer Number Example 4 Part 1: Growing food for cattle 35 25 20 15 10 5 0 Bench Fridge Warm cupboard Temperature Example 5 20.0 18.0 16.0 14.0 12.0 10.0 8.0 6.0 4.0 2.0 Bench Fridge Warm cupboard Temperature Example 6 36 Cattle production 6 5 4 3 2 1 0 Bench Fridge Warm cupboard Temperature Example 7 Part 1: Growing food for cattle 37 Suggested answers Suggested answers The rate of photosynthesis If the rate of photosynthesis is increased, the rate of growth and development will also increase. Questions 1 Statements a, c, e and g are true. Statements b, d, f and h are false. 2 3 38 b Photosynthesis takes place in the green parts of plants. d Water is taken into the plant through the roots. f As the intensity of light increases, the rate of photosynthesis will only increase while no other factors are limiting. h Water availability does have an effect on the photosynthetic rate. You should name two of the three factors named below. Then you should describe how each of your chosen factors will limit the photosynthetic rate of the crop and finally the growth of the crop. • light is essential as the energy to drive the photosynthesis reaction. If plants have their amount of light decreased then the energy for driving photosynthesis is decreased and hence the growth of the plant is decreased. • water is an essential reactant in the photosynthesis reaction. If the amount of water is limited then photosynthesis is limited and therefore the growth of the plant will be limited. • decreased soil fertility, particularly decreased levels of phosphorus can limit photosynthesis. Phosphorus is essential for the photosynthesis reaction to proceed. Therefore if photosynthesis is reduced then plant growth will be reduced. Cattle production 4 Some farmers producing crops such as tomatoes prune back the leaf cover to reduce the effects of shading. When the leaves are in shade they are unable to carry out photosynthesis and hence are not producing food for the plant. These leaves although they are not producing food are using up valuable resources of water, nutrients and the products of photosynthesis but are unable to contribute to the growth of the plant. Hence they are removed. Photosynthesis and respiration 1 Sunlight is essential for this process to occur. P 2 This process occurs 24 hours a day. R 3 The process that produces a continuous supply of available energy. R 4 Carbon dioxide is needed for this process. P 5 This process occurs only in the green parts of plants. P 6 This process occurs in all living cells. R Rates of photosynthesis and respiration 1 Five am until seven pm 2 Twenty four hours a day. 3 Eight am to four pm. 4 It means that this is the period when there is more food produced than is broken down to produce energy. Achieving maximum NAR Below is a list of some of the management practices that a farmer could carry out to maximise the NAR of crop plants. You only need to have outlined three of these. • Ensure adequate water for the crop, by irrigating, mulching the soil surface or increasing the organic matter content of the soil. • Ensure adequate soil fertility, particularly of the nutrients nitrogen, phosphorus, sulphur and potassium, by adding fertilisers, adding compost and ensuring the correct pH level so that all nutrients are available. • Try to increase the proportion of photosynthetic material on the plant. This means that rate of photosynthesis can outstrip the rate of respiration. Part 1: Growing food for cattle 39 • For crops grown in glasshouses, artificial lighting can be used to increase the photoperiod. • For crops grown in glasshouses, temperature can be manipulated to increase the daytime temperature and decrease the night temperature. • The crop can be pruned to reduce the amount of shading of leaves so that all leaves can have the chance to photosynthesise. Otherwise the shaded leaves will be respiring but not photosynthesising and hence wasting energy. Experimental design factors 1 a) The size of the seed could affect how well the plant grows (this becomes another variable in the experiment). Treatments A and B may not have any real difference, but the difference in seed size could alter the result. b) There are two ways that you could approach this; you could randomly allocate the seed to the pots or you could randomly allocate the pots to treatment. Both would remove bias. You could number all the pots and put the numbers on pieces of paper in a bag. Drawing out the pot number indicates that it is the next to have a seed placed in it. You could fill all the pots and then toss a coin to decide if each pot is treatment A or B. 2 a) The amount of water the pots receive may cause one group to grow better than the other and thus alter the results of the experiment. b) The researcher should avoid positioning the pots from a single treatment as a block because will always be variations in the environment. It would be best to try and fit all the pots on one side of the greenhouse. Even then there could be uneven levels of water (or other environmental variations), so the researcher should try to randomly allocate the pots to the area. This could be done by numbering the positions and the pots, then writing the numbers on paper and putting them in two containers. Simultaneously drawing a number from each container will randomly match a position with a pot. Standardisation The soil should be as uniform as possible, including texture, structure, pH, slope, nutrient levels, organic matter levels and depth of topsoil. The previous cropping history should be the same over the area. Shading and windbreaks should be avoided as they provide uneven protection across the site. If irrigation is provided it should be uniform. The same methods of cultivation, sowing, fertiliser application, pest and disease control and harvesting should be used. 40 Cattle production Data collection Plant or animal Part measured Units cabbages cabbage head kilograms/ hectare layer chickens eggs number of eggs/chicken/year wheat grain tonnes/hectare beef cattle weight gain kilograms/head/year Wheat variety experiment The usual variety Cunningham is included in the trial to act as a control to compare with the new variety, Sunco. If Cunningham was not included in the trial then the experimenter would not be able to tell if the new variety was better or not. The fields are not randomly allocated to the treatments. This means that the experiment could be biased by slight differences in the conditions in each field. The implication is that the outcome of the experiment may be altered and the results unreliable. The experimenter should use a random method such as a coin toss to decide which field is planted to each variety. The experiment has no replication, which means that the results are open to error. Something unexpected such as insect damage to one field can easily alter the outcome. Differences or errors in the way the two fields are managed will not be distinguishable from differences caused by the treatments. To overcome this the experimenter should divide the available fields into smaller areas so that there are at least four replicates of each treatment. These eight smaller plots should then be allocated randomly to treatments. The experiment appears to be well standardised; with every aspect kept as similar as possible, including soil qualities and management operations. It is important to maintain this high level of standardisation as it will ensure the validity of the outcome, making the result more reliable. Without standardisation any differences may be due to factors other than the treatment. The experiment has used an appropriate form of data collection, the weight of the grain indicates how useful each variety will be in a commercial setting as this is the valuable agricultural product. Weight is also a practical measurement to collect as after harvesting the grain is Part 1: Growing food for cattle 41 routinely weighed to determine payment. Collecting data on other aspects of crop growth such as leaf length would be difficult and would not provide useful data. yield (vegetative) Planting density density Based on Lovett et al, 1988, p 47 Experimental Design 1 Planting density. 2 The treatments provide a comparison against each other, so they act as controls for each other. An absence of oat plants is not a reasonable option in this experiment. 3 The seeds should be randomly allocated to pots. Pots should be randomly allocated to positions. This can be done by allocating pots to treatments, numbering the pots, then drawing numbers from a hat to decide which pot gets seed next. The numbers in the hat can be drawn again to decide the positioning of the pots in the available area. 4 A minimum number of replicates for each treatment is probably four pots. Four different densities are likely to give an indication of the optimum level. This would give a total number of pots as 4 x 4 = 16 pots. If your resources allow you might consider a larger and more accurate trial. 42 Cattle production Presentation Example 1 – table presents all the raw data groups data from the different locations together makes it seem that the saucer number is the variable does not include processing of the data such as mean or standard deviation includes total germinated, which is not relevant or useful. Example 2 – table presents all the raw data groups data from the different locations together clearly indicates that the locations are the variable includes mean and standard deviation Example 3 – graph presents all the raw data makes it seem that the saucer number is the variable Example 4 – graph presents all the raw data makes it seem that the saucer number is the variable joining the points makes it seem that the data could be continuous instead of being distinct measurements poor choice of scale means that little difference can be seen between the results Example 5 – graph presents all the raw data clearly shows that the location is the variable groups the results from each location together so they can be compared – can easily see the range of results for each Part 1: Growing food for cattle 43 Example 6 – graph allows easy comparison of locations does not show all data, so cannot see how variable the results are for each location the scale emphasizes the difference between the locations by starting at 2 instead of 0 Example 7 – graph This type of graph is sometimes included in reports in addition to other graphs, with the mistaken belief that more is better. alone does not really show anything useful to somebody trying to understand the data joining the points makes it seem that the data could be continuous instead of being distinct Pastures 1 Carbohydrates provide animals with energy. 2 Protein is needed by animals for growth and production. Native pasture Native pastures • Do not require high levels of inputs like fertiliser and irrigation. • Well adapted to the specific areas where found. • Persistent in adverse conditions, like low rainfall, extreme heat or fire. Introduced Pastures 44 • High producing and faster growing. • More digestible and palatable. • Seed easier to collect, clean and sow. Cattle production Pasture management • Select pasture species that are suited to the local environmental conditions to reduce the quantity of inputs to the system. • Use native pasture species to reduce the use of inputs such as fertiliser and irrigation. • Pasture species can be used that assist in the control of pests or diseases of other crops. For example, lucerne pasture controls skeleton weed, a weed pest of wheat. • Sowing pest or disease resistant pasture varieties means better productivity and less use of chemical control measures. For example aphid resistant lucerne varieties. • When sowing use direct drilling rather than a cultivated seedbed, to avoid damaging soil structure and causing erosion. • Use computerized grazing management tools to make optimum decisions. • Education and skills updating of managers so they are able to select the best management strategies in a situation. Part 1: Growing food for cattle 45 Exercises – Part 1 Exercises – Part 1 Exercises 1.1 to 1.2 Name: _________________________________ Exercise 1.1: Grazing oats experiment Present your experiment as a formal report. Language Try to use correct language and not slang. When you are writing about something you have done; avoid writing I did this.. Use the style called third person, and write instead this was done.. as if you watched somebody else doing it. A good way to check if you are doing this correctly is that the word I should not appear in your report at all. If you are still not sure about the type of language you are expected to use, then read through some published experimental reports or talk to your teacher. Aim The aim should be a short statement about the hypothesis that the experiment is testing. _________________________________________________________ _________________________________________________________ _________________________________________________________ 46 Cattle production Method The method should clearly show how the experiment has been set up. You may find a diagram is helpful. Include a description of how experimental design principles are used. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Part 1: Growing food for cattle 47 Results Present your raw data as a table and a graph. 48 Cattle production Discussion This is the main section of the report, where you can show your understanding by using critical analysis and drawing out the implications of the experiment. • Outline what your results show. Interpret the results. Are the results showing what you expected? If not, why not? • Discuss how effective your experimental design was, and suggest how it could be improved in future experiments of the same kind. Include both the good and bad aspects of your experiment. • Compare your results to the expected results. Include discussion of why your graph is, or is not, the same as the graph of vegetative yield and density in the answers section. • Draw out the implications of the experimental results on management practices in agriculture. Include the economic implications of your findings. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Part 1: Growing food for cattle 49 _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ 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_________________________________________________________ _________________________________________________________ _________________________________________________________ Conclusion The conclusion should be a short statement that summarises what you found out by doing the experiment with regard to the original aim. _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Part 1: Growing food for cattle 51 Exercise 1.2: Wallaby grass 1 Identify the benefits of Wallaby grass for the environment. ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ 2 Outline the most important of the benefits of using wallaby grass in agriculture. ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ 3 Identify two major barriers to the domestication of a native grass. ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ ______________________________________________________ 4 52 Produce a fact sheet that briefly provides the important information on how best to establish and manage domesticated wallaby grass. Use point form, you do not need to use full sentences. Include subheadings to break up the information. Cattle production Fact Sheet: Establishment and management of domesticated wallaby grass _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ _________________________________________________________ Part 1: Growing food for cattle 53
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