Organelle Lights™ Intracellular Targeted Fluorescent Proteins
*BacMam 1.0*
Table 1. Contents and storage information.
Material
Amount
Organelle Lights™ reagent* (Component A)
6 mL
BacMam enhancer (Component B)
Not disclosed
Dimethylsulfoxide (DMSO, Component C)
120 µL
Storage
• 2–6˚C
• Desiccate
• Protect from light
• DO NOT FREEZE
Stability
When stored as directed, the
product is stable for at least
6 months.
*Approximate fluorescence excitation/emission maxima: CFP: 435/485 nm; GFP: 485/520 nm; RFP: 555/584 nm. CFP and GFP can be
seen through a FITC filter; Cy3® or TRITC filters are acceptable for RFP.
Introduction
The Organelle Lights™ reagent contains a baculovirus that, upon entry into mammalian cells,
directs the expression of autofluorescent proteins that are localized to specific subcellular
compartments and organelles via a signal peptide or protein fusions (Table 2).
Baculoviruses are insect cell viruses. The use of baculovirus for protein production in insect
cells has been used extensively for over two decades; however its application to mammalian
cells is relatively new. The utilization of baculovirus to deliver genes to mammalian cells is
referred to as BacMam technology, and has been recently reviewed.1,2
The BacMam technology has several key features: 1) high transduction efficiency across
a broad range of cell types, including primary, stem, and neuronal cells, 2) minimal
microscopically observable cytopathic effects, 3) highly reproducible and titratable expression,
4) biosafety level 1 reagent as it will not replicate in mammalian cells, and 5) simultaneous
delivery of multiple genes. Overall, the BacMam technology is a straightforward method of
gene delivery with few or no observable side effects.
The Organelle Lights™ reagent combines the content of a targeted fluorescent protein with the
viral delivery of the BacMam technology for simple, specific, and robust protein expression in
mammalian cells.
The Organelle Lights™ reagent allows visualization of specific parts of living cells using
fluorescence microscopy, flow cytometry, or high content imaging (HCI). Each kit contains a
virus solution with a targeting sequence fused to a fluorescent protein gene sequence already
incorporated into the viral genome and an enhancer solution for increased expression of the
chimera. If the potential effects of baculovirus on your cell type are a concern, use Cellular
Lights™ Null (Control) reagent that lacks any mammalian genetic elements. The null (control)
reagent allows determination of potential baculovirus-mediated effects and background
fluorescence.
MAN0002459 Revised: 26–May–2010 | MP 36209
Note: Cellular Lights™ and Organelle Lights™ reagents utilize the first generation of BacMam
delivery technology, BacMam 1.0. Recent improvements to this system, including the addition
elements that greatly enhance transduction efficiency and expression levels, has warranted
the conversion of these reagents to the next generation products, CellLight™ BacMam 2.0.
Conversion of the Cellular Lights™ and Organelle Lights™ BacMam 1.0 to CellLight™ BacMam
2.0 is expected to be completed by the end of 2010. Refer to www.invitrogen.com for current
availability.
Table 2. Organelle Lights™ reagent targeting information and available colors.
Organelle or subcellular
structure
Targeting sequence
Reference
CFP*
GFP*
RFP*
Endoplasmic reticulum (ER)
ER signal sequence of calreticulin and KDEL (ER retention signal)
3
X
X
Golgi
Human Golgi-resident enzyme N-acetylgalactosaminyltransferase 2
4
X
X
Plasma membrane (PM)
Myristoylation/palmitoylation sequence from Lck tyrosine kinase
5
X
X
Mitochondria
Leader sequence of E1alpha pyruvate dehydrogenase
6
X
X
Nucleus
SV40 nuclear localization sequence
7
X
X
Peroxisomes
Peroxisomal C-terminal targeting sequence
8
X
Cytoplasm
Nuclear export sequence
9
X
Synaptic veiscles
Synaptophysin
10
X
X
Lysosomes
Lamp1 (lysosomal associated membrane protein 1)
11
X
X
Endosomes
Rab5a
12
X
X
X
X
*CFP: Cyan Fluorescent Protein; GFP: Green Fluorescent Protein; RFP: Red Fluorescent Protein.
Figure 1. Schematic diagram of Organelle Lights™ reagent workflow.
Organelle Lights™ Intracellular Targeted Fluorescent Proteins | 2
Materials Required but Not
Provided
Dulbecco’s Phosphate Buffered Saline (D-PBS) without Ca2+ or Mg2+
Cell types successfully transduced using BacMam delivery technology include: HeLa, CHO
M1WT3, CHO K1, COS-7, SKBR3, HepG2, U-2OS, NIH 3T3, HEK 293, HUVEC, rat
primary astrocytes, LAA progenitor cells (adult stem cells), primary cardiac muscle cells,
primary skeletal muscle cells, NT2 human embryonal carcinoma cells, human mesenchymal
stem cells, porcine cardiac stem cells, primary human arterial smooth muscle cells, primary
human lung fibroblasts, rat neuronal stem cells, and spiral ganglial neurons. Currently, the
delivery system does not work well for hematopoietic or macrophage cells. For a complete list
of cells compatible for use with Cellular Lights™ and Organelle Lights™, refer to our website
(www.invitrogen.com).
Before You Begin
1.1 To use the Organelle Lights™ transduction reagents, plate ~1 × 106–4 × 106 mammalian cells
per well in a 100-mm dish. Allow cells to adhere and grow for approximately 4–24 hours at
37°C, 5% CO2 before proceeding with the transduction (see Figure 1 for an overview of the
transduction process). Optimal transduction occurs when cells are 70–80% confluent.
1.2 Prepare the BacMam enhancer solution. Reconstitute the entire vial of enhancer
(Component B) in 25 µL DMSO (Component C) for a 1,000X solution.
Note: The enhancer solution is stable for multiple freeze/thaw cycles under the recommended
storage conditions. If desired, you may divide the solution into aliquots following reconstitution.
Experimental Protocols
Transduction with Organelle
Lights™ Reagent
If desired, you may try a range of reagent concentrations around the dilution given below
(see step 2.1) in your initial transduction experiments with a particular cell type to determine
the optimal amount of reagent to use. For all colors of Organelle Lights™ reagents, too little
virus will result in transduction of only a few cells. The novel TagRFP is a monomeric red
fluorescent protein obtained from the sea anemone Entacmaea quadricolor, and is about
three times brighter than mCherry13, making it the brightest available monomeric red
fluorescent protein. The CFP and emerald GFP (emGFP) are mutants of the original Aequoria
victoria GFP.
2.1 Prepare 5.5 mL of Organelle Lights™ transduction solution in Dulbecco’s Phosphate Buffered
Saline (D-PBS) without Ca2+ or Mg2+ by combining 2.0 mL of Organelle Lights™ reagent
(Component A) with 3.5 mL of D-PBS.
If you wish to transduce your cells with multiple Organelle Lights™ reagents at the same time,
substitute the additional volume of the reagents for the D-PBS. For example, for transducing
with two Organelle Lights™ reagents combine 2.0 mL of each Organelle Lights™ reagent with
1.5 mL D-PBS. For transducing with three reagents, combine equal amount of each reagent
(1.8 mL of each).
Note: Always protect the Organelle Lights™ transduction reagent (Component A) from light
and place the vial back at 4°C as soon as possible after each use. Exposure to light over time
will decrease the viral titer. Titration of the Organelle Lights™ reagent and/or enhancer may
lead to improved results.
Organelle Lights™ Intracellular Targeted Fluorescent Proteins | 3
2.2 Aspirate the cell culture medium from a 100-mm dish containing ~1 × 106–4 × 106 adherent
cells, and add 5.5 mL of the diluted Organelle Lights™ transduction solution to the plate. Incubate
the cells at room temperature (20–25°C) in the dark for 2–4 hours with gentle rocking or
shaking.
Note: Some cell types (i.e., primary and stem cells) are sensitive to lack of calcium and
magnesium, and will begin to detach in their absence. You may shorten the incubation
time to 15–30 minutes with these cell types; however, this may decrease the transduction
efficiency.
2.3 Aspirate Organelle Lights™ transduction solution from the cell culture dish.
2.4 Add appropriate cell culture medium with or without serum plus 1X enhancer (i.e., 10 µL of
enhancer per 10 mL media). Incubate the cells for 2 hours in optimal growth conditions (i.e.,
37°C and 5% CO2).
2.5 Aspirate enhancer solution from the cell culture dish and replace with the appropriate cell
culture medium. Incubate cells at 37°C, 5% CO2 for >16 hours to allow for the expression of
the Organelle Lights™ fluorescent protein.
2.6 After incubation, you may perform imaging on live or fixed cells.
2.7 Optional: At this stage, you may trypsinize the cells, freeze in Recovery™ Cell Culture
Freezing Medium, and store in liquid nitrogen for future use.
Imaging the Transduced Cells
3.1 Image cells expressing Organelle Lights™ fluorescent proteins using a fluorescence
microscope or other suitable fluorescence-imaging instrument. Approximate fluorescence
excitation and emission wavelengths in nanometers are: 435/485 for CFP, 485/520 for GFP,
and 555/584 nm for RFP. You may use a FITC filter for CFP and GFP, and a Cy3™ or TRITC
filter for RFP. Refer to Figure 2 for excitation and emission spectra of GFP, CFP, and RFP.
Note: You may observe fluorescence from Organelle Lights™ proteins 16–24 hours following
transduction, which generally persists for more than 5–7 days. Some variation in expression
level from cell to cell within the same culture is normal.
GFP
CFP
A
RFP
B
C
Figure 2. Fluorescence excitation and emission spectra for Organelle Lights™ Green Fluorescent Protein (GFP, panel A), Organelle Lights™ Cyan Fluorescent Protein (CFP,
panel B), and Organelle Lights™ Red Fluorescent Protein (RFP, panel C), obtained in PBS, pH 7.4 (for GFP and CFP). The RFP spectra was determined in 100 mM NaCl,
20 mM Tris-HCl, pH 7.5.
Organelle Lights™ Intracellular Targeted Fluorescent Proteins | 4
3.2a Optional: You may fix your cells with paraformaldehyde as Organelle Lights™ fluorescence
is well-retained after fixation. To fix, treat cells with 4% paraformaldehyde solution in PBS
for 10–30 minutes at room temperature. If desired, you may permeabilize the cells following
fixation with 0.2% Triton® X-100 in PBS for 5 minutes at room temperature.
Note: Do not use methanol. The fluorescence may be lost. However, if methanol fixation is
necessary for downstream processing, you may detect CFP and GFP proteins using anti-GFP
antibodies and RFP using the anti-RFP antibody, available separately from Invitrogen.
3.2b Following fixation, wash cells by performing two sequential 5 minute washes in PBS. You may
image the cells in PBS, or, for increased photostability, place the cells in ProLong® Gold antifade reagent for imaging.
References
1. Nature Biotech 23, 567 (2005); 2. TRENDS in Biotech 20, 173 (2002); 3. FEBS Letters 405, 18 (1997); 4. J Cell Biol 143, 1505 (1998); 5. EMBO 16,
4983 (1997); 6. J Biol Chem 279, 13044 (2004); 7. TiBS 16, 478 (1991); 8. J Cell Biol 108, 1657 (1989); 9. BMC-R 2, 1 (2005); 10. J Neuroscience 26, 3604 (2006);
11. J Cell Sci 118, 5243 (2005); 12. J Biol Chem 284, 29218 (2009); 13. Nat Biotechnol 12, 1567 (2004).
Organelle Lights™ Intracellular Targeted Fluorescent Proteins | 5
Product List Current prices may be obtained from our website or from our Customer Service Department.
Cat. no.
Product Name
Unit Size
C10080
Organelle Lights™ Synaptophysin-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O10098
Organelle Lights™ Golgi-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O10099
Organelle Lights™ Nuc-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O10100
Organelle Lights™ Lysosomes-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O10104
Organelle Lights™ Endosomes-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O10138
Organelle Lights™ Synaptophysin-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O10139
Organelle Lights™ PM-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36209
Organelle Lights™ Nuc-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36210
Organelle Lights™ Mito-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36211
Organelle Lights™ Peroxi-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36212
Organelle Lights™ ER-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36214
Organelle Lights™ PM-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36215
Organelle Lights™ Golgi-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36216
Organelle Lights™ PM-CFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36218
Organelle Lights™ Nuc-CFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36227
Organelle Lights™ Cyto-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36228
Organelle Lights™ Lysosomes-GFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36229
Organelle Lights™ Mito-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36230
Organelle Lights™ ER-RFP *BacMam 1.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
O36231
Organelle Lights™ Endosomes-RFP *BacMam 2.0* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
Related Products
A10262
anti-green fluorescent protein, chicken IgY fraction (anti-GFP, IgY) *2 mg/mL* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 µL
A11120
anti-green fluorescent protein, mouse IgG2a, monoclonal 3E6 (anti-GFP, mAb 3E6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 µg
A11121
anti-green fluorescent protein, mouse IgG1, monoclonal 11E5 (anti-GFP, mAb 11E5) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 µg
A11122
anti-green fluorescent protein, rabbit IgG fraction (anti-GFP, IgG) *2 mg/mL* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 µL
A21311
anti-green fluorescent protein, rabbit IgG fraction, Alexa Fluor® 488 conjugate (anti-GFP, IgG, Alexa Fluor® 488 conjugate) *2 mg/mL* 100 µL
A31852
anti-green fluorescent protein, rabbit IgG fraction, Alexa Fluor® 647 conjugate (anti-GFP, IgG, Alexa Fluor® 647 conjugate) *2 mg/mL* 100 µL
B10107
BacMam Enhancer Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
B10383
BacMam 2.0 GFP Transduction Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 mL
C10078
Cellular Lights™ CSF1r-GFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10079
Cellular Lights™ Exoc1-YFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10105
Cellular Lights™ MAP4-GFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10106
Cellular Lights™ Tubulin-GFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10112
Cellular Lights™ Tubulin-RFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10126
Cellular Lights™ Actin-GFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10127
Cellular Lights™ Actin-RFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1 kit
C10128
Cellular Lights™ Histone 2B-GFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10129
Cellular Lights™ Histone 2B-RFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10130
Cellular Lights™ Null (Control) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10140
Cellular Lights™ MAP4-RFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10323
Cellular Lights™ Talin-GFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10324
Cellular Lights™ Talin-RFP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
G10362
GFP, ABfinity™ recombinant rabbit polyclonal antibody - unconj (anti-GFP, rabbit mAb) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 μg
P36930
ProLong® Gold antifade reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 mL
R10367
RFP, rabbit polyclonal antibody - unconjugated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 μg
12648-010
Recovery™ - Cell Culture Freezing Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mL
14190-136
Dulbecco’s Phosphate Buffered Saline (D-PBS) without Ca2+ or Mg2+ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1,000 mL
Organelle Lights™ Intracellular Targeted Fluorescent Proteins | 6
Contact Information
Molecular Probes, Inc.
29851 Willow Creek Road
Eugene, OR 97402
Phone: (541) 465-8300
Fax: (541) 335-0504
Customer Service:
6:00 am to 4:30 pm (Pacific Time)
Phone: (541) 335-0338
Fax: (541) 335-0305
[email protected]
Toll-Free Ordering for USA:
Order Phone: (800) 438-2209
Order Fax: (800) 438-0228
Technical Service:
8:00 am to 4:00 pm (Pacific Time)
Phone: (541) 335-0353
Toll-Free (800) 438-2209
Fax: (541) 335-0238
[email protected]
Invitrogen European Headquarters
Invitrogen, Ltd.
3 Fountain Drive
Inchinnan Business Park
Paisley PA4 9RF, UK
Phone: +44 (0) 141 814 6100
Fax: +44 (0) 141 814 6260
Email: [email protected]
Technical Services: [email protected]
Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly
from Molecular Probes. Customers in Europe, Africa and the Middle East should contact our office in Paisley, United Kingdom. All others
should contact our Technical Service Department in Eugene, Oregon.
Molecular Probes products are high-quality reagents and materials intended for research purposes only. These products must be used
by, or directl­y under the super­vision of, a tech­nically qualified individual experienced in handling potentially hazardous chemicals. Please
read the Material Safety Data Sheet provided for each product; other regulatory considerations may apply.
Limited Use Label License No. 223: Labeling and Detection Technology
The manufacture, use, sale or import of this product may be subject to one or more patents or pending applications owned or licensed
by Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased
amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or
for-profit entity) in a manner consistent with the accompanying product literature. The buyer cannot sell or otherwise transfer (a) this
product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or
its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information
or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial
Purpose, and that such collaborator agrees in writing (a) to not transfer such materials to any third party, and (b) to use such transferred
materials and/or information solely for research and not for Commercial Purposes. Commercial Purposes means any activity by a party
for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product
or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or
prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for
use in research. For products that are subject to multiple limited use label licenses, the most restrictive terms apply. Life Technologies
Corporation will not assert a claim against the buyer of infringement of patents that are owned or controlled by Life Technologies
Corporation and/or Molecular Probes, Inc. which cover this product based upon the manufacture, use or sale of a therapeutic, clinical
diagnostic, vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed,
provided that neither this product nor any of its components was used in the manufacture of such product. If the purchaser is not
willing to accept the limitations of this limited use statement, Life Technologies is willing to accept return of the product with a full
refund. For information on purchasing a license to this product for purposes other than research, contact Molecular Probes, Inc., Business
Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Limited Use Label License No: 21 Bac-to-Bac® and Bac-to-Bac® HT
This product is the subject of U.S. Patent No. 5,348,886. This product is sold under patent license from Monsanto for research purposes
only and no license for commercial use is included. Requests for licenses for commercial manufacture or use should be directed to
Director, Monsanto Corporate Research, 800 N. Lindbergh, St. Louis, Missouri 63167.
Limited Use Label License No: 127 GFP with Heterologous Promoter
This product and its use is the subject of one or more of U.S. Patent Nos. 5,491,084 and 6,146,826, and foreign equivalents. This product is
sold under license from Columbia University. Rights to use this product are limited to research use only, and expressly exclude the right
to manufacture, use, sell or lease this product for use for measuring the level of toxicity for chemical agents and environmental samples
in cells and transgenic animals. No other rights are conveyed. Not for human use or use in diagnostic or therapeutic procedures. Inquiry
into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia
Innovation Enterprise, Columbia University, Engineering Terrace-Suite 363, New York, New York 10027.
Limited Use Label License No: 267 Mutant GFP Products
This product and its use is the subject of one or more of U.S. Patent Nos. 6,090,919, 5,804,387, 5,994,077, and foreign equivalents.
Limited Use Label License No: 272 Humanized GFP
This product is the subject of one or more of U.S. Patent Numbers 5,786,464, 5,795,737, 5,874,304, and 6,114,148 and foreign equivalents
licensed by Invitrogen Corporation. This product is sold for research use only. Not for therapeutic or diagnostic use in humans.
Limited Use Label License No: 306 Baculovirus Vectors
Certain methods that utilize the product associated with this limited use label license are covered by U.S. Patent Nos. 5,731,182 and
5,871,986. This product is for research use only by those researchers in laboratories of academic, government, industrial and/or clinical
institutions engaged in the investigation of biological or biochemical processes, or research and development of biological products. This
product is not to be used in the manufacture, use or sale of human or animal diagnostic, therapeutic or prophylactic products.
Limited Use Label License No: 307 GFP Cell Sensors
This product and its use is the subject of one or more of U.S. Patent Nos. 5,777,079, 6,066,476, and 6,319,669 and foreign equivalents. The
purchase of this product conveys to the buyer the non-transferable right to use the purchased product only for use with mammalian cells
and not for use with other cell types. No rights are conveyed to modify or clone the gene encoding GFP contained in this product, or to
replicate this product in any cell line.
Limited Use Label License No: 321 TagRFP
This product is for the buyer’s internal research use only and may not be used for commercial purposes. No rights are conveyed to modify
or clone the gene encoding fluorescent protein contained in this product. The right to use this product specifically excludes the right
to validate or screen compounds for commercial purposes. For information on commercial licensing, contact Licensing Department,
Evrogen JSC, email: [email protected].
Several Molecular Probes products and product applications are covered by U.S. and foreign patents and patents pending. All names
containing the designation ® are registered with the U.S. Patent and Trademark Office.
The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.
Copyright 2010, Life Technologies Corporation. All rights reserved. This information is subject to change without notice.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Organelle Lights™ Intracellular Targeted Fluorescent Proteins | 7