BioChek - Newsletter January 2011
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January 2010
BIOCHEK: NEWS
News: New registrations in the USA
Employee profile: Wick
Interpretation News: NDV Case History of Infection with Vaccination Index
Product news: Poultry, Swine and ringtrial samples
BioChek Calendar
Seminars/Training
Trade shows/Conferences
NEW REGISTRATIONS IN THE USA
BioChek Mg, Ms, MgMs and AI type A antibody detection kits USDA registered!
In December 2010 BioChek received registration from the United States Department
of Agriculture (USDA) for Mg, Ms, MgMs and AI antibody detection test kits.
We currently can supply from our distribution centre in Gorham, Maine (USA) the
following test kits:
Mg CK114 Mycoplasma gallisepticum antibody test kit
Ms CK115 Mycoplasma synoviae antibody test kit
MgMs CK215 Mg/Ms combination antibody test kit
AI CK121 type A avian influenza antibody test kit
NDV CK116 Newcastle disease antibody test kit
ILT CK124 Infectious Laryngotracheitis antibody test kit
We expect to have IBD, IBV, REO, AE, CAV and Salmonella to be registered early 2011!
Please send orders or requests for quotation for delivery in the USA to www.biochek.com or contact Gwen Slacum at:
03025215554 or by e-mail: [email protected]
EMPLOYEE PROFILE: WICK DE BRUIJN
1. What does the logistics department do?
We process orders (pack-and ship), manage the inventory and manage the financial
aspect of each order. In addition we communicate with customers and account
managers about any special request.
2. Who is Wick?
I am 42 years old, live in Gouda and ride my bicycle to the office every day. I enjoy
jogging and riding my motorbike. I have 2 children and a dog. I empty the
dishwasher at BioChek every day, something I refuse to do at home.
3. Please describe your job and tasks. What is it exactly that you do?
Sandra takes care of the administrative part of the orders, I take care of packing and shipping and customers relations. In
addition I’m also responsible for inventory management, not an easy task in a growing business.
4. What do you find interesting in your job?
It’s fun being part of a relatively small and informal organization. The international aspect of my work dealing with customers
all over the world creates an extra challenge. Furthermore getting the products delivered in good order and on time is very
satisfying.
5. What do you expect to achieve for BioChek in the coming two years?
We want to contribute to the success of BioChek. It’s very exciting specially when looking at the growth in the number of
current products and the addition of the new product line, the swine tests. This will surely have a huge impact on the number
of orders and the total volume of kits to be shipped.
6. What are your personal goals?
Attending The World cup in Brazil! To which we will go with the entire BioChek team if we manage to double sales in the
coming 3 years!
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INTERPRETATION NEWS:
NDV CASE HISTORY OF INFECTION WITH VACCINATION INDEX
In the April newsletter we have discussed Vaccination Index (VI) as a new approach
to evaluate the antibody titer response following vaccination/infection. To show the
ease of interpretation of using VI, we have decided to show a case history of a
confirmed clinical NDV infection in Broilers, following 2x live NDV application (at 01D
spray vaccination in Hatchery and at 20D drinking water application). The VI
guideline for NDV live vaccination for Broilers at 45D is given below:
Vac Program
VI Normal
VI Suspect Infection
Up to 3x Live vaccine
50- 250
>300
Despite vaccination with 2x live vaccination, at 21D onset of mortality and clinical symptoms was noted. Birds display severe
respiratory stress, nervous symptoms, paralysis of legs, and neck twisting. At post mortem birds show Airsacculitis and lesions
on Proventriculus and guts. Total mortality varied from 15 -21% in affected flocks. ELISA Serology done on all infected flocks
for all respiratory diseases (IBV, NDV, MG, MS, ORT, AI and ILT), revealed significant VI scores for NDV, indicating infection.
To demonstrate the ease interpretation with the help of VI an example of a non-infected and clinically infected flock is given
below:
NDV: normal vaccination serology in BR (VI index in normal range 50 -250)
Case: broilers were vaccinated on day 1 with live NDV Coarse Spray and on day 20 with live vaccine through Dr water. Shown
below is normal serology at 45 days.
NDV: infection serology in BR (VI index > 300)
Case: broilers were vaccinated on day 1 with live NDV Coarse Spray and on day 20 with live vaccine through Dr water. At 21
days of age birds showed clinical symptoms of respiratory infection and nervous symptoms (neck twisting) coupled with 21%
mortality. Shown below is serology at 45 days.
The good news is that the VI index is now included as an option in the BioChek software and will be automatically calculated
and printed on the reports.
If you like to use the VI index in your software or like to know more on VI, please contact BioChek or a nearest BioChek
representative.
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PRODUCT NEWS:
Poultry
Swine
Ringtrial samples
POULTRY
New testkits:
Group 1 Avian Adenovirus antibody detection kit (product code CK132)
New accessories:
F09 prediluted reference control (download insert from http://www.biochek.com/index.php?id=65)
RS03 lyophilized reference serum (download insert from http://www.biochek.com/index.php?id=65)
BioChek ringtrial sample set
Group 1 Avian Adenovirus antibody detection kit
Classification:
Genus Aviadenovirus (Group 1 Avian Adenovirusses)
Species:
Serotypes:
FAV A
serotypes 1
FAV B
serotypes 5
FAV C
serotypes 4 & 10
FAV D
serotypes 2, 3, 9 & 11
FAV E
serotypes 6, 7, 8a & 8b
Background:
The role of most Group 1 Avian Adeno virusses as pathogens is not well defined. However FAV 4 is associated with
hydropericardium syndrome. Strains from several species but particularly from species E are related to the condition known as
Inclusion Body Hepatitis (IBH).
Intended use:
To determine presence or absence of FAV Group 1 antibodies in Poultry the test will give semi-quantitative results.
Kit format:
Product code CK 132
5 plates per kit
Run at room temperature
Incubation times: 30-30-15
Read at 405 NM
Indirect ELISA
1:100 dilution
Ranges RS09
BioChek ELISA Test
Target Titer Range
ELISA IBD
4 000 - 8 000
ELISA IBV
2 000 - 6 000
ELISA NDV
6 000 - 10 000
ELISA ART
3 000 - 7 000
ELISA REO
2 000 - 6 000
ELISA AE
2 000 - 6 000
ELISA CAV
2 000 - 7 000
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ELISA MG
1 000 - 4 000
ELISA MS
1 500 - 4 500
ELISA MG/MS
Value on COA
ELISA EDS
500 - 3 000
ELISA Salmonella Group D
500 - 3 000
ELISA Salmonella Group B and D
Value on COA
ELISA AI
1 500 - 5 000
ELISA ILT
1 500 - 5 500
Ranges RS03
RS03 Freeze Dried Reference Serum - Target Titers
RS03 lot number
CH5446
RS03 expiry date
September-2013
ELISA brand
BioChek
BioChek ELISA Test
Target Titer Range *
ELISA IBD
6 000 – 10 000
ELISA IBV
4 000 –
ELISA NDV
8 000
11 000 – 16 000
* Titers range data has been formulated from multiple runs with different kit lots
SWINE
New testkits:
Hps OppA antibody detection kit
PCV2 antibody detection kit
AI multispecies
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Reference control:
• SR01 reference control. For use on currently available Swine kits.
HpsOppA antibody detection kit
General information about the test:
The BioChek Hps OppA ELISA is based on an intracellular protein called OppA. OppA is expressed by all Hps serotypes and it is
regarded to be specific for Hps only. Expression of OppA is only after clinical infections, despite of the Hps serotype, or after
vaccinations.
Intended use of the test:
Detection of clinical infected animals diagnosis
Detection of protective immunity
Prediction of time of vaccination
Confirmation of success of vaccination
Kit format:
Product code: SK104
2 plates per kit, 16 well strips
Run at room temperature
Incubation times: 60-60-15
Read at: 405NM
Indirect ELISA
1:50 dilution
PCV2 antibody detection kit
General information about the test:
Inactivated PCV2 virus is used as antigen. This antigen will detect antibodies directed against all strains of PCV2. Antibodies
due to natural infection, maternal antibodies and antibodies due to vaccination will be detected.
Intended use of the test:
Detection of infected animals
Herd profiling and prediction of time of vaccination
Confirmation of success of vaccination
Kit format:
Product code: SK105
5 plates per kit, 16 well strips
Run at room temperature
Incubation times: 30-30-15
Read at: 405NM
Indirect ELISA
1:50 dilution
AI type A antibody detection kit
General information about the test:
The format is a blocking ELISA which will detect antibodies against all Influenza A viruses. By this design antibodies against all
SIV serotypes will be detected. Both antibodies from natural infections and from vaccination will be detected.
Intended use of the test:
Detection of infected animals
Confirmation of success of vaccination
Kit format:
Product code: CK 401
5 plates per kit
Run at room temperature
Incubation times: 60-30
Read at: 405NM
Indirect ELISA
1:50 dilution
Software
The new software, BioChek ll, has new reports specially designed for swine monitoring:
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Farm Histogram: this report will depict a histogram with the results on each sample taken from the various types of pigs in a
house.
NEW: BIOCHEK RINGTRAIL SAMPLES
BioChek now has a panel of 12 freeze dried serum samples with known values. The samples have been validated on IBD, IBV
and NDV.
You can run these samples on either one of these tests.
After running these samples send the results back to BioChek and we will send you our results and the interpretation back.
When samples give the expected results we will send a certificate. In the case that the results are out of range you will be
contacted by BioChek and we will help identifying the cause for the inaccuracy.
The objective of this ringtrial is to confirm accuracy and reproducibility of the laboratory.
Product code: RT 001, price € 50 per set
Batch no: RT001
Send order, together with a regular order to: [email protected]
Remarks: feedback can and will only be given when used on BioChek kits!
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BIOCHEK CALENDAR
SEMINARS / TRAINING
BioChek laboratory technician training sessions for 2011 will be on the following dates:
Thursday May 12th and Friday May 13th.
Thursday September 22nd and Friday September 23rd.
The training sessions are meant for laboratory technicians using BioChek ELISA kits.
The focus is on:
Pipetting technique
Accuracy of results
Equipment maintenance
The use of the BioChek software
At the end of the session the participants will receive a certificate. The maximum amount of participants is 12, the minimum is
6. The training takes 2 days. The language is English.
Venue: BioChek Training Laboratory, Reeuwijk (NL).
Program:
Day 1
12:00
13:00
Group
Group
19:00
Lunch
Start training session
A running assays
B software
Dinner
Day 2
8:00 Group A/B running assays, Group A/B software
12:30 Lunch
13:30 Equipment maintenance/calibration of pipettes
14:30 Wrap up
15:00 Departures
To make a reservation for one of these sessions please send an e-mail with the following information:
Name
Company name
Phone Number
E-mail
Participants: 1 - 2
Selected training date
Arrival time/date:..............................................
Departure time/date:..............................................
You can send this information to: [email protected]
(Sandra is also available for questions and more information).
TRADE SHOWS / CONFERENCES
Upcoming events:
January 26 - 28, 2011 IPE Atlanta Georgia, USA
Booth 1828 Hall A
Please come and visit us during IPE in Atlanta. On Thursday the 27th we will host a lunch seminar from 12:00 - 13:30.
Please contact Gwen Slacum ([email protected]) or Mariangela Manfredini ([email protected]) when
you want to attend the seminar. Topics are: Assessing Salmonella vaccination of breeder pullets with the BioChek Se/St ELISA
kit by Dr John Smith of Fieldale Farms and Vaccination index, a new concept for data interpretation by Dr Bart van Leerdam,
BioChek
March 9 – 11, 2011 VIV ASIA Bangkok, Thailand
Booth C025
September 6-9, 2011 XXII Latin American Poultry Congress
La Rural convention center in Buenos Aires, Argentina
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