MAR111 Exercise 5 (10 points) Name _________________________________ Lower Invertebrates - Porifera, Cnidaria, Ctenophora, Annelida Porifera (Sponges) 1. Observe the whole dried sponge specimens. Notice the large number of pores and the spongy texture that comes from the protein spongin. Now look at a slide of spongin using a compound microscope. Describe what spongin looks like. ____________________________ ____________________________________________________________________________ 2. Below is a diagram of the anatomy of a simple sponge showing the spicules and 4 types of cells (porocytes/pore cells, pinacocytes/epithelial cells, amoebocytes/wandering cells, and choanocytes/collar cells). Use your book and/or your lecture notes to help you identify those 5 items on the diagram: A = _________________________ B = _________________________ A C = _________________________ E D = _________________________ D E = _________________________ B C 1 3. Use a compound microscope to look at the slide showing a whole mount of the sponge Grantia. Draw what you see and label the pores, osculum opening, and spicules. 4. Look at the slide of loose sponge spicules using a compound microscope. What are the two possible types (chemical compositions) of spicules? ____________________________________ ___________________________________ What could you add to the spicules to determine which kind they are? (Hint: remember the algae lab chemicals). __________________________________________________________ Draw the spicules you observed: Cnidaria (Jellyfishes, Anemones, Corals) There are 4 classes of cnidarians: Hydrozoa, Scyphozoa, Cubozoa, and Anthozoa. You will see examples of all except Cubozoa. 5. Use a compound microscope to look at the slide of the colonial hydrozoan Obelia. Draw the organism and label the stem, polyps, and tentacles. 2 6. Gonionemus is a hydrozoan jellyfish. Look at the preserved Gonionemus jellyfish, draw a side view of it below, label the bell and tentacles, and show the oral and aboral sides. 7. Look at the plastic embedded specimen of Physalia (Portuguese man-of-war), which is a colonial siphonophore, not a jellyfish. Using a jellyfish for comparison (Gonionemus or Aurelia), name 2 ways that the Portuguese man-of-war is different from a jellyfish. ____________________________________ ___________________________________ 8. Corals and anemones are anthozoans. Hard corals have a polyp body shape like anemones, but secrete a calcium carbonate skeleton. Look at some of the coral skeletons available. The living coral animal used to live on top of that skeleton, with each polyp sitting inside a cup. Solitary coral Colonial star coral Colonial brain coral In living colonial corals, the polyps are interconnected by thin sheets of tissue called coenosarcs. Inside the polyps, there is no stomach because cnidarians have no organs, but there are digestive mesenterial filaments. Most shallow, tropical corals have symbiotic photosynthetic microorganisms living inside their tissues which provide additional nutrition and which remove wastes from the coral. What are these microorganisms? ____________________________________ 3 Label the skeleton, tentacles, mesenterial filaments, mouth, and coenosarc on the diagram below. Also show the oral and aboral sides. 9. View the slide of nematocyst stinging cells using a compound microscope. They will look like this: undischarged discharged What are two functions of the stinging cells of cnidarians? ____________________________________ ___________________________________ Ctenophora (Comb Jellies) 10. Look at the preserved specimens of Pleurobrachia comb jellies using a dissecting microscope to see better detail. Comb jellies get their name because they are gelatinous and have 8 rows of ciliated combs that run along their bodies. They also have 2 tentacles, which may not be present because they may have broken off during the capture and preservation of the samples. 4 Draw a comb jelly below and label a few representative rows of cilia combs. 11. Using a jellyfish for comparison (Gonionemus or Aurelia) and what you learned in lecture, name 2 ways (other than the cilia combs) that comb jellies are different from jellyfish: ____________________________________ ___________________________________ Annelida (Segmented Worms) 12. Look at the preserved Nereis worm using a dissecting microscope to see better detail. Note the walking appendages which are called parapodia on each side of each segment. The ends of the parapodia should have bristles (setae). These bristles are characteristic of the polychaetes (meaning "many bristles") that are the dominant type of annelid worm in the oceans. Draw the head and several segments of the worm. Identify the segments, parapodia, and bristles/setae. Summary 13. Each of the 4 phyla examined today represent a different body symmetry. Identify the symmetry exhibited by each phylum. (Use your book and/or lecture notes.) Porifera = ______________________ Cnidaria = _____________________ Ctenophora = ______________________ Annelida = ______________________ 5
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