IOWA STATE UNIVERSITY
OF SCIENCE AND TECHNOLOGY
2011 IOWA EVALUATION OF INSECTICIDES
AND
PLANT-INCORPORATED PROTECTANTS
CORN PESTS RESEARCH PROJECT
DEPARTMENT OF ENTOMOLOGY
AMES, IOWA 50011-3140
SUE BLODGETT, CHAIR
INSECTS INVESTIGATED
Corn Rootworm
Report Authors
Aaron Gassmann
Patrick Weber
European corn borer
Corn Earworm
April 10, 2012
File number: 289-11
2011 IOWA EVALUATION OF INSECTICIDES AND
PLANT INCORPORATED PROTECTANTS
TABLE OF CONTENTS
Page
1. Research Personnel .......................................... 1
2. Introduction and Objective ............................... 2
3. Testing Procedures and Evaluations............... 2
4. Studies Conducted .......................................... 13
5. Corn Rootworm Evaluation Tables
Ames
Pioneer CMRA study
Root Injury/Percent Consistency ........... 14
Stand Count .......................................... 14
Yield ...................................................... 15
Pioneer dual pyramid efficacy and yield for corn rootworm
Stand Count .......................................... 15
Root Injury/Percent Consistency ........... 16
Yield ...................................................... 16
Monsanto corn rootworm study
Root Injury/Percent Consistency ........... 17
Stand Count .......................................... 17
Yield ...................................................... 18
Monsanto genuity VT3P seed blend study
Stand Count .......................................... 18
Yield ...................................................... 19
Table of Contents (Continued)
Page
Ames
Monsanto smartstax features and benefits study
Stand Count .......................................... 19
Yield ...................................................... 20
Ames (Old Dairy Farm)
Syngenta experimental hybrid efficacy study
Root Injury/Percent Consistency ........... 20
Ames (South Dairy Farm)
Syngenta experimental hybrid efficacy study
Root Injury/Percent Consistency ........... 21
Crawfordsville
Yield study
Root Injury/Percent Consistency ........... 21
Stand Count .......................................... 22
Yield ...................................................... 22
Monsanto smartstax refuge in bag study
Stand Count .......................................... 23
Yield ...................................................... 23
Monsanto smartstax features and benefits study
Stand Count .......................................... 24
Yield ...................................................... 24
Northeast Iowa
Monsanto corn rootworm study
Root Injury/Percent Consistency ........... 25
Stand Count .......................................... 25
Percent Lodging .................................... 26
Yield ...................................................... 26
Nashua
Yield study
Root Injury/Percent Consistency ........... 27
Stand Count .......................................... 27
Percent Lodging .................................... 28
Yield ...................................................... 28
Table of Contents (Continued)
Page
Monsanto corn rootworm study
Root Injury/Percent Consistency ........... 29
Stand Count .......................................... 29
Percent Lodging .................................... 30
Yield ...................................................... 30
6. European Corn Borer Evaluation Tables
Ames
Pioneer Optimum AcreMax II for European corn borer
Stand Count .......................................... 31
ECB Whorl Injury ................................... 31
ECB Tunneling (cm) .............................. 32
Yield ...................................................... 32
7. Corn Earworm Evaluation Tables
Ames
Dow AgroSciences smartstax vs. HXX & RR2 corn traits study
Stand Count .......................................... 33
Percent Damaged Kernels .................... 33
Kernel Area Consumed (cm2) ................ 33
Total Damaged Ears ............................. 33
Monsanto smartstax features and benefits study
Stand Count .......................................... 34
Percent Damaged Kernels .................... 34
Kernel Area Consumed (cm2) ................ 34
Total Damaged Ears ............................. 34
8. Appendix I - Agronomic Information ............. 35
9. Appendix II – Weather Data............................ 41
10. Appendix III - Materials Tested ..................... 50
11. Appendix IV - Research Pictures ................. 54
2011 IOWA EVALUATION OF INSECTICIDES AND
PLANT INCORPORATED PROTECTANTS
CORN PESTS RESEARCH PROJECT
DEPARTMENT OF ENTOMOLOGY
AMES, IOWA 50011-3140
Project Leader:
Aaron Gassmann
Agricultural Specialist:
Patrick Weber
Postdoctoral Research Associate:
Jennifer Petzold-Maxwell
Graduate Research Assistants:
Mike Dunbar
Ryan Keweshan
Eric Clifton
Amanda Hoffmann
Lab Research Assistant:
Jeremy Bolles
Technical Assistants:
Charlie Ethington
James Terry
Lee Koenigsfeld
Phil Johnson
Walter Wohlford-Wessels
University Research
Farm Superintendents:
Kent Berns
Ryan Rusk
Kenneth Pecinovsky
Kevin VanDee
2 INTRODUCTION
The corn rootworm species, which
includes the western corn rootworm
Diabrotica
virgifera
virfigera
and
northern corn rootworm D. barberi, are
the most damaging pests of corn Zea
mays in the Unites States Corn Belt.
Eggs are laid in the soil during the fall
and hatch the following spring. Larval
feeding on corn roots in June may
diminish yield by reducing plant growth
and drought tolerance and by imposing
harvesting losses due to plant lodging.
Adult emergence from the soil is
underway by early July and continues
through late summer. Additional crop
losses can be caused by the beetles
feeding on the female flowers (silks) and
on soft doughy kernels. In Iowa, crop
rotation, where it fits cropping practices,
remains the preferred method of control.
However, it is also economically feasible
to protect corn roots with insecticides
and
plant-incorporated
protectants
(transgenic seedcorn that contains a
gene from the naturally occurring soil
bacterium Bacillus thuringiensis (Bt).
In additional to corn rootworm,
several
above-ground
lepidopteran
pests feed on corn in Iowa. These
include the European corn borer
Ostrinia nubilalis, black cutworm Agrotis
ipsilon, fall armyworm Spodoptera
frugiperda,
and
corn
earworm
Helicoverpa zea. Over the last decade,
Bt corn varieties have been made
commercially available to protect corn
from these pests.
OBJECTIVE
The goal of this research program is to
serve Iowa agriculture by monitoring
and evaluating the performance of
registered commercial insecticides and
transgenic corn hybrids. To achieve this
goal, we maintain a viable, proactive,
progressive and scientifically sound
product evaluation program.
TESTING PROCEDURES AND
EVALUATIONS
Field Sites: Product efficacy study plots
were established at six Iowa locations in
2011. Corn rootworm (CRW) research
fields are continually maintained on
University farms located at: Ames,
Johnson Farm; Crawfordsville, S.E.
Research and Demonstration Farm;
Nashua,
N.E.
Research
and
Demonstration Farm; Sutherland, N.W.
Research and Demonstration Farm and
Lewis,
S.W.
Research
and
Demonstration Farm. Each research
field is divided into two sections which
annually alternate as test plot and late
planted trap crop. The seed planted for
the trap crop is a mixed maturity blend
with a greater proportion of latematuring varieties. This trap crop
constitutes a favorable environment for
adult female CRW late in the season
when other fields are maturing. In
addition, we conducted corn rootworm
(CRW) research on two second year
corn fields at two Ames locations (ISUOld Dairy Farm and ISU South Dairy
Farm). Also, we conducted corn
rootworm (CRW) research on a
continuous corn field (since 2003) in a
private grower’s field in northeastern
Iowa.
Table 1: Lists the research conducted at
each location, target pest, and other
general plot information.
3 Corn Rootworm Studies
Field plot design: The experimental
design in most studies was a
randomized complete block. Treatments
in
the
yield
studies
at
both
Crawfordsville (Tables 17-19) and
Nashua (Tables 28-31) were paired
rows 75-feet in length with four
replications. Treatments for the Pioneer
dual pyramid study for corn rootworm at
Ames (Tables 5-7) were four rows wide
with 40 feet per row with four
replications. The Pioneer CMRA study
(Tables 2-4) at Ames were eight row
treatments that were 25 feet in length
with six replications. The treatments in
the Monsanto corn rootworm studies at
Ames (Tables 8-10), northeast Iowa
(Tables 24-27) and Nashua (Tables 3235), were eight row treatments with 40
feet per row with four replications. The
Monsanto genuity VT3P study in Ames
(Tables 11-12) were eight row
treatments with 100 feet per row with
one
replication.
The
Monsanto
smartstax features and benefits study in
Ames (Tables 13-14) were eight row
treatments with 200 feet per row with
one replication.
Treatments for the
Syngenta experimental hybrid efficacy
studies at Ames-ISU Old Dairy Farm
(Table 15) and Ames-ISU South Dairy
Farm (Table 16) were four row wide
treatments that were 20 feet in length
with four replications. The Monsanto
smartstax RIB study in Crawfordsville
(Tables 20-21) were eight row
treatments with 100 feet per row with
one
replication.
The
Monsanto
smartstax features and benefits study in
Crawfordsville (Tables 22-23) were eight
row treatments with 100 feet per row
with one replication.
Application techniques: Seeds were
pre-bagged and planted with a four-row
John Deere Max EmergeTM 7100
integral planter that had 30 inch row
spacings.
The
standard
planter
fiberglass seed hoppers with attached
“finger pickup mechanism,” were
replaced with modified units. On the
new units, the metal plate that covered
the “fingers” had been replaced with a
7/8-inch, clear Plexiglas plate. Inserted
through the Plexiglas was a small
stainless steel cylinder. The cylinder
was positioned to deliver seed to the
“pickup fingers.” At the beginning of
each replication pre-bagged seeds were
dumped into the steel cylinder. At the
beginning of each replication, a
hydraulic motor (attached to the
planter’s drive shaft) was activated to
deliver seed immediately into the
ground. At the end of each replication,
this same hydraulic motor was activated
to expel any unplanted seed.
Plant-incorporated protectants: Plantincorporated protectants were evaluated
in corn hybrids producing insecticidal
toxins derived from the bacterium
Bacillus thuringiensis (Bt). These
included the YieldGard hybrids of
Monsanto that produce the Bt toxin
Cry3Bb1 and the Herculex hybrids of
Pioneer and Dow AgroSciences that
produce
the
binary
Bt
toxin
Cry34/Cry35.
Seed treatments: All the DeKalb
hybrids evaluated in the yield studies
(Crawfordsville, Tables 17-19; Nashua,
Tables 28-31) were treated by the
respective company with Poncho 500.
All the Mycogen hybrids evaluated in the
yield studies (Crawfordsville, Tables 1719; Nashua, Tables 28-31) were treated
by the respective company with Cruiser
4 Extreme® 250. For the Pioneer dual
pyramid study for corn rootworm,
(Ames, Tables 5-7) all treatments were
treated with Cruiser® 250 except one
treatment of the pure stand of RR2
where the seeds were treated with
Poncho 1250/VOTIVO. For the Pioneer
CMRA study (Ames, Tables 2-4) for
corn rootworms and Pioneer Optimum
AcreMax II study (Ames, Tables 36-39)
for European corn borer all hybrids were
treated with Cruiser® 250. For the
blended refuge seeds (RR2) planted in
the Pioneer dual pyramid study for corn
rootworm all seeds were commercially
treated with Poncho 1250/VOTIVO. In
the Pioneer Optimum AcreMax II study
for European corn borer all blended
refuge seeds (RR2) planted were
treated with Cruiser® 250 insecticidal
seed treatment. For the Monsanto
studies at Nashua (Tables 32-35), Ames
(Tables 8-10) and northeast Iowa
(Tables 24-27), Monsanto Genuity VT3P
study in Ames (Tables 11-12),
Monsanto smartstax features and
benefits study at Ames (Tables 13-14)
and Crawfordsville (Tables 22-23), and
Monsanto smartstax RIB study in
Crawfordsville (Tables 20-21) the
Poncho 250 seed treatment was
commercially applied. The Syngenta
experimental hybrid efficacy studies at
Ames-Old Dairy Farm (Tables 15) and
Ames-South Dairy Farm (Tables 17)
these hybrids were treated with Cruiser
Extreme® 250.
All these seed treatments target
secondary soil-borne pests, such as
wireworm and seedcorn maggot. For
some treatments, plant-incorporated
protectants
were
combined
with
conventional insecticides.
Granular
application
treatments:
Force 3G granular insecticide was
applied to one treatment in the Pioneer
dual pyramid study for corn rootworm
study and six treatments in each of the
Syngenta experimental hybrid efficacy
studies at Ames-Old Dairy Farm and
Ames-South Dairy Farm with modified
Noble® metering units mounted on the
planter. Aztec 2.1G granular insecticide
was applied to two treatments in the
Yield studies at both Crawfordsville and
Nashua with modified Noble® metering
units mounted on the planter. The Noble
units were calibrated in the laboratory to
accurately deliver material at a tractor
speed of 4 mph. Plastic tubes directed
the granular treatments to either a 7inch band ahead of the closing wheels
(T-band, All-Terrain Banders), or to the
seed furrow, placing all the insecticide
in-furrow (Furrow). Eleven-inch polybristle skirts were attached to the frame
and the frame positioned so the bristle
tips touched the ground. Each row was
constantly monitored to ensure that
insecticides were correctly applied at all
times.
Final
incorporation
was
accomplished with drag chains mounted
behind the closing wheels.
SmartBoxTM application treatments:
SmartChoice 5G and Counter 20G,
treatments were applied with modified
SmartBoxTM metering units. These
products were applied as ounces per
1000 row feet. The commercial
SmartBoxTM were removed from their
large-base containers and sandwiched
between a flat metal plate on the bottom
and a custom-made, threaded plastic
cap on the top. The bottom plate had
been fabricated so that it could slide in
and out of the same planter mounting
brackets used for the Noble units. An
inverted 1000 mL Nalgene bottle,
screwed into the top cap provided a
secure and sealed container for
5 insecticide. A short plastic tube attached
to the dispenser opening of the metering
unit could be connected to either the
planter’s T-band or Furrow tubes. The
two controllers mounted in the tractor
cab were used to operate the
SmartBoxTM
metering
units.
All
treatments were applied at 4 mph using
the “fixed speed mode” on the
SmartBoxTM controllers.
European Corn Borer Study
Field plot design: The experimental
design in the Pioneer Optinum AcreMax
II study for European corn borer was a
randomized complete block with four
replications
respectively.
Five
treatments were evaluated (Pioneer
HX1 alone, Pioneer RR2 hybrid alone,
Pioneer HX1 x YGCB (pyramid) alone,
and with a 5% and 10% blended refuge
seeds (RR2). These treatments were
four rows wide, 40-feet in length. Plots
were cut down to 38 feet of row for alley
purposes. In the blended refuges, a
gene check was used to identify refuge
plants (rows 1 and 4: blended refuge
treatments only). A total of twenty four
three-plant clusters were marked with
stakes for rows 1 and 4 of the 5%
blended refuge treatment and the 10%
blended refuge treatment. Two six-plant
clusters were marked with stakes within
rows 1 and 4 for the treatments of HX1
hybrid alone, HX1 x YGCB (pyramid)
and RR2 hybrid alone for all four
replications. Within the blended refuge
treatments the plant clusters consisted
of either: Refuge-Bt-Bt or Bt-Bt-Refuge.
Application techniques: The ECB
neonates were obtained from the
USDA-ARS Corn Insects and Crop
Genetics Research Laboratory. We
obtained four bottles of neonates that
were mixed with corn grit. We infested
with first-generation ECB on 17 June
and 24 June. The inoculum was applied
into the whirl. When infesting with
second-generation ECB on 9 August
and 16 August, the inoculum was
applied into the ear-leaf collar.
Inoculum was equal to 100 ECB per
plant and administered with bazooka
inoculators.
On 15 July, the ECB injury was
scored. A total of 288 plants were
evaluated. We used the Pioneer 9-1
rating scale for evaluating firstgeneration ECB whorl injury.
On 6 October and 7 October,
tunneling was evaluated by splitting
stalks into three parts (top, shank, and
bottom) with a curved linoleum knife. All
three plant clusters in the two blended
refuge treatments and all six plant
clusters in the three pure stand
treatments were evaluated for a total of
288 plants. Total tunnel length (cm) was
scored for each plant part and averaged
for each treatment.
Corn Earworm Study
Field plot design: The experimental
design in this Dow AgroSciences
smartstax vs Herculex XTRA and RR2
corn study in Ames was a randomized
complete block with four replications.
The treatments consisted of Mycogen
RR2 corn, Mycogen smartstax and
Mycogen Herculex XTRA. These
treatments were four rows wide, 20-feet
in length.
Application techniques: The 1st CEW
instar larvae were obtained from Benzon
Research from Carlisle, Pennsylvania.
Prior to infestation, corn ear shoot bags
were placed over the top ear shoot of all
6 plants in row 2 and 3 of each plot. On 4
August a #2 camel hair brush was used
to infest 10 ears per row (of rows 2 and
3) to the silks with 5, 1st instar larvae.
One summer Technical Assistant was
assigned one replication of treatments
to infest.
On 18 August (14 days after
infestation) 10 randomly selected ears
were sampled from the two center rows
of each plot. From these ears we
counted the numbers of kernels
damaged, in addition, we measured with
a caliper (in millimeters) the average
kernel cap size, both length and width.
In addition, we counted the number of
rows of kernels around the ear and the
number of kernels in an average row
length. From these numbers collected,
we calculated the percentage of
damaged kernels per ear and per
treatment.
were placed over the top ear shoot of 10
plants in row 2 and 7 of each plot. On 10
August a #2 camel hair brush was used
to infest 10 ears per row (of rows 2 and
7) to the silks with 5, 2nd instar larvae.
One summer Technical Assistant was
assigned one replication of treatments
to infest.
On 24 August (14 days after
infestation) 10 randomly selected ears
were sampled total from rows 2 and 7 of
each plot. From these ears we counted
the numbers of kernels damaged, in
addition, we measured with a caliper (in
millimeters) the average kernel cap size,
both length and width. In addition, we
counted the number of rows of kernels
around the ear and the number of
kernels in an average row length. From
these numbers collected, we calculated
the percentage of damaged kernels per
ear and per treatment.
Corn Earworm Study
Corn Rootworm Larval
Evaluations
Field plot design: The experimental
design in this Monsanto smartstax
features & benefits study was a
randomized complete block with four
replications. The treatments consisted of
Pioneer HXX (Pioneer 35F34PDR),
Syngenta
Agrisure
(N53C-3111),
DeKalb smartstax (DKC 55-09), DeKalb
RR2 (DKC 55-08) with Aztec 2.1G 0.12
ounces ai/1000 rowft, Syngenta GT
Agrisure (N53-W2) with Aztec 2.1G 0.12
ounces ai/1000 rowft, Pioneer RR2
(35F37) with Aztec 2.1G 0.12 ounces
ai/1000 rowft and DeKalb RR2 (DKC 5508) These treatments were eight rows
wide, 200-feet in length.
Application techniques: The 1st CEW
instar larvae were obtained from Benzon
Research from Carlisle, Pennsylvania.
Prior to infestation, corn ear shoot bags
Stand counts: The number of plants in
either 17.5, or 20 row-ft was recorded
(note distance in foot notes of stand
count tables). These were taken early
and late in the growing season.
The number of plants in 38 row-ft
was recorded for both the Pioneer Dual
Pyramid study for corn rootworm and
Pioneer Optimum AcreMax II study for
European corn borer. These were taken
early and at Physiological maturity
during the growing season.
Root-Injury:
Since
CRW
beetle
emergence was delayed by 10-14 days,
we delayed starting root digging until the
first of the month of August. After the
majority of corn rootworms had finished
feeding, roots were dug from 8/01/2011
7 through 8/17/2011. Five root systems
total were dug per replication from each
insecticide treatment (except from the
My-95%smartstax/5%non-Bt treatment
(refuge in the bag)) where nine root
systems (6 smartstax + 3 non-Bt) total
were dug per replication from the tworow yield studies (Crawfordsville, Table
17 & Nashua, Table 28). For the
Monsanto corn rootworm studies at
Nashua (Table 32), Ames (Table 8) and
northeast Iowa (Table 24), five root
systems total were dug from each
insecticide treatment out of rows 2 and
7. With the Pioneer CMRA study, six
roots total were dug per treatment from
rows two and seven (Ames, Table 3).
With the Pioneer dual pyramid study for
corn rootworm, we dug 48 root systems
per treatment from the four pure stand
treatments (HXX + MIR604, RR2 Hybrid
+ Force 3G at 4 oz/1000feet of row,
RR2 hybrid with Poncho 1250/VOTIVO
insecticidal seed treatment, RR2 hybrid
with Cruiser 250 insecticidal seed
treatment. 72 root systems per
treatments were dug from the two
blended refuge treatments (HXX +
MIR604 (pyramid), with 5% blended
refuge seeds-P0413R, HXX + MIR604
(pyramid) with 10% blended refuge
seeds-P0413R). Roots were dug out of
rows 1 and 4 only. With the Syngenta
experimental hybrid efficacy study, a
total of six root systems total were dug
from rows one and four of each
treatment (Ames-Old Dairy Farm- (Table
15) and Ames-South Dairy Farm-(Table
16). Prior to leaving the field, the roots
were marked with a permanent marker
with the plot number, study name and
location. Excess soil was removed in the
field as well. In Ames, roots were
soaked in water and then a pressurized
water spray was used to remove the
remaining soil. Roots were then laid out
by replications and were then evaluated
for rootworm feeding injury on the
following Iowa State Node-Injury Scale
(0-3):
Node-Injury Scale (0-3):
0.00 - No feeding damage (lowest rating
that can be given).
1.00 - One node (circle of roots), or the
equivalent of an entire node,
eaten back to with-in ~ 1½
inches of the stalk (soil line on
the 7th node).
2.00 - Two nodes eaten.
3.00 - Three or more nodes eaten.
(Highest rating that can be given)
Damage in-between complete nodes
eaten is noted as the percentage of the
node missing, i.e. 1.50 = 1½ nodes
eaten, 0.25 = ¼ of one node eaten, and
so on.
The linear node-injury scale allows
injury to be expressed intuitively and
has proved useful in evaluating minor
injury, especially with transgenic seed
corn. For an interactive guide to the
Node-injury scale, see the Iowa State
University Entomology web site at:
rootworm/nodeinjury/nodeinjury.html
8 means with an experiment wise error
rate of P < 0.05.
COMMENTS ON INSECTICIDES AND
PLANT INCORPORATED
PROTECTANTS (PIP)
PERFORMANCE
Product consistency: The percent
product consistency was also calculated
for each treatment. Product consistency
equals the percentage of times a
treatment limited feeding injury to 0.25
node or less (greater injury can result in
economic yield loss, especially when
plants are moisture stressed).
Lodging counts:
A plant was
considered lodged if it was leaning at
least 30 degrees from vertical. These
were taken at harvest time along with
final stand count numbers (note
distance in footnotes of lodging count
tables).
Yields: Studies that were taken to yield
were machine harvested with a modified
John Deere 9410 plot combine or John
Deere 9450. Weights (pounds) and
percent moisture were recorded from
either a HarvestMaster brand or
Shivvers brand 5010 moisture meter
plot harvest data collection system.
These measurements were converted to
bushels per acre of No. 2 shelled corn
(56 lbs. per bushel) at 15% moisture in
an Excel worksheet.
All data were analyzed with standard
ANOVA
procedures
using
SAS
Enterprise Guide 4.2.
When a
significant treatment effect was present
pairwise comparisons made among
Tables list treatment rates as ounces
active ingredient per 1000 row foot
unless otherwise indicated in the
footnotes.
CORN ROOTWORM EVALUATIONS
AMES (Johnson farm)
Pioneer CMRA study (Tables 2-4):
Both the Herculex XTRA treatments
(with and without Bio-insecticide) had
significantly less node-injury than the
two Herculex 1 treatments (Table 2).
The Herculex 1 alone treatment had a
significantly lower stand count than the
other three treatments (Table 3). No
significant differences in yield were
detected (Table 4).
Pioneer dual pyramid yield and
efficacy for corn rootworm (Tables 57): No significant differences were noted
for stand counts (Table 5). Root injury
was lowest and product consistency
greatest for RR2 with Force 3G.
Product consistency was lowest for RR2
in the 10% blended refuge treatment. In
general the pyramid had lower root
injury and higher product consistency
than RR2, except for RR2 with Force 3G
(Table 6). The pure stand of RR2 with
Poncho 1250/VOTIVO insecticidal seed
treatment was the highest yielding and
was significantly greater than the pure
stand of RR2 with the Cruiser250
insecticidal seed treatment and the
pyramid with a 10% blended refuge.
(Table 7).
9 AMES (Johnson farm)
Monsanto corn rootworm study
(Tables 8-10): The Mycogen Herculex
XTRA treatment was the same as the
DeKalb RR2 hybrid treatment but was
significantly different than all other
treatments for node-injury (Table 8).
Regarding product consistency, the
Mycogen Herculex XTRA treatment was
the same as the smartstax treatment but
both were significantly different than the
remainder of the treatments (Table 8).
With stand counts all treatments were
the same except the DeKalb YieldGard
VT3 + Force overtop and DeKalb RR2
hybrid which were significantly different
from each other (Table 9). No significant
differences in yield were detected (Table
10).
Monsanto
genuity
VT3P
study
(Tables
11-12):
No
significant
differences were noted for stand counts
(Table 11). No significant differences in
yield were detected (Table 12).
Monsanto smartstax features and
benefits study (Tables 13-14): No
significant differences were noted for
stand counts (Table 13). No significant
differences in yield were detected (Table
14).
AMES (Old Dairy Farm)
Syngenta
experimental
hybrid
efficacy study (Tables 15): Rootworm
pressure was low but we did see a
significant difference with the Non-Bt
hybrid showing greater node-injury
compared to the other treatments (Table
15).
AMES (South Dairy Farm)
Syngenta
experimental
hybrid
efficacy study (Tables 16): Rootworm
pressure was low but we did see a
significant difference with the Non-Bt
hybrid showing greater node-injury
compared to the other treatments (Table
16).
CRAWFORDSVILLE (S.E. R&D FARM)
Yield study (Tables 17-19): Node injury
was significantly higher for the two
isoline treatments (checks) than all other
treatments. The treatment of Mycogen
Herculex HXX with SmartChoice-SB
insecticide In-furrow over top was the
same as the DeKalb Smartstax with
Aztec 2.1G overtop treatment and the
DeKalb Smartstax with Aztec 2.1G
overtop was the same as Mycogen HXX
plus Force 3G overtop and the Mycogen
Smartstax treatments (Table 17).
Regarding stand counts, the Mycogen
Isoline hybrid was the same as all
treatments except the Mycogen 95%
Smartstax/5%Non-Bt
which
was
significantly different (Table 18). With
yield the Mycogen Isoline hybrid was the
same as all other treatments except the
Mycogen HXX with Force 3G and
SmartChoice 5G SmartBox applied Infurrow overtop (Table 19).
Monsanto smartstax RIB study
(Tables
20-21):
No
significant
differences were noted for stand counts
(Table 20). No significant differences in
yield were detected either (Table 21).
Monsanto smartstax features and
benefits study (Tables 22-23): The
Agrisure
hybrid
(Agrisure
3111)
treatment was significantly different than
all other treatments for stand counts
10 (Table 22). No significant differences in
yield were detected (Table 23).
Northeast Iowa
Monsanto corn rootworm study
(Tables 24-27): There was heavy CRW
pressure at this site. The DeKalb Isoline
Hybrid (DKC 44-92) treatment had the
highest node-injury followed by the
DeKalb YieldGard VT3 hybrid (DKC 4660) followed by the Mycogen Herculex
Hybrid (DKC 2K592) treatment and
finally the DeKalb Isoline Hybrid (DKC
44-92) treatment with Force overtop.
These four treatments were significantly
different than all the other treatments
(Table 24). No significant differences
were noted for stand counts (Table 25).
The DeKalb Isoline Hybrid (DKC 44-92)
and the DeKalb YieldGard VT3 hybrid
(DKC
46-60)
treatments
had
significantly greater percent lodging than
all other treatments (Table 26). No
significant differences in yield were
detected (Table 27).
NASHUA (N.E. R&D FARM)
Yield study (Tables 28-31): The
treatment of DeKalb-Smartstax with
Aztec 2.1G overtop In-furrow had
significantly less node-injury than all ten
other treatments (Table 28). No
significant differences among stand
counts were noted (Table 29).
Regarding percent lodging, the DeKalb
Isoline and Mycogen Isoline hybrid
treatments were significantly different
than all other treatments (Table 30). For
yield, the DeKalb Isoline and Mycogen
Isoline
hybrid
treatments
were
significantly different than all other
treatments (Table 31).
Monsanto corn rootworm study
(Tables 32-35): The smartstax hybrid
(DKC 46-61) treatment had significantly
less
node-injury
than
all
other
treatments. Both the YieldGard VT3
hybrid (DKC 46-60) treatment and
DeKalb Isoline (DKC 61-72) treatment
with either Aztec or Force overtop had
significant less node-injury than either
one of these hybrids alone (Table 32).
The smartstax hybrid (DKC 46-61)
treatment had significantly lower stand
count compared to the Mycogen
Herculex hybrid (DKC 2K592) and
DeKalb Isoline hybrid (DKC 61-72) plus
Force applied overtop treatments (Table
33). The DeKalb Isoline Hybrid (DKC
61-72) treatment had significantly
greater percent lodging than all other
treatments (Table 34). For yields, the
Mycogen Herculex Hybrid (DKC 2K592)
treatment was significantly greater than
all other treatments in this study (Table
35).
EUROPEAN CORN BORER
EVALUATIONS
AMES (Johnson farm)
Pioneer Optimum AcreMAX II study
for European corn borer (Table 3639): Stand counts were similar among
all treatments (Table 36). With whorl
injury, the RR2 plants within the 10%
blended refuge were significantly
greater than the other treatments (Table
37). Tunneling of ECB was significantly
lower on HX1 and the pyramid (HX1 x
YGCB) than on RR2 either alone or in a
blended refuge (Table 38). No
significant differences in yield were
detected (Table 39).
CORN EARWORM EVALUATIONS
AMES (Johnson farm)
Dow Agro Sciences Smartstax vs.
Herculex XTRA and RR2 corn traits
11 study (Tables 40-42): The HXX/RR2
treatment had significantly lower stand
count than the RR2 but was similar to
the smartstax treatment (Table 40).
Regarding damaged kernels per ear,
percent damaged per ear, kernel area
consumed (cm) and total damaged ears
both the RR2 and HXX/RR2 treatments
were significantly higher than the
smartstax treatment (Tables 41-42).
AMES (Johnson farm)
Monsanto smartstax features and
benefits
study
(Tables
43-44):
Regarding damaged kernels per ear,
percent damaged per ear, kernel area
consumed (cm) and total damaged ears,
both the Agrisure hybrid (Agrisure 3111)
and smartstax (DKC 55-09) treatments
were significantly lower than all other
treatments (Tables 43-44).
CALIBRATION INFORMATION
All Noble® units were laboratory
calibrated and units were randomly
spot-checked in the field prior to
planting.
SmartBoxTM
units
were
calibrated on the planter in accordance
with the SmartBox Operator’s Manual
instructions. During calibration and
planting, the flowability of each
formulation was noted, as well as any
other calibration problems. There were
no calibration or delivery problems with
any treatment.
AGRONOMIC INFORMATION,
WEATHER DATA AND
MATERIALS TESTED AND PICTURES
OF RESEARCH PROJECTS
Agronomic
information
and
field
insecticide history for each test plot
location are listed in Appendix I.
Weather data from the test site or the
nearest Iowa Climatological Station are
listed in Appendix II. Information on
materials tested is listed in Appendix III.
Pictures of various activities of research
projects are listed in Appendix IV.
RESEARCH SUPPORT
Many thanks to the Iowa Agriculture and
Home Economics Experiment Station
and the following companies for
providing support for the evaluation of
insecticides
and
plant-incorporated
protectants (Corn Pests Research
Project): AMVAC Chemical Corporation,
Dow AgroSciences, Monsanto, Pioneer
Hi-Bred International, Inc., Syngenta.
WAIVER OF ENDORSEMENT
This report deals with the relative ability
of each treatment to protect corn from
damage by soil insects. This information
is not presented to endorse the use of
any product and the name of Iowa State
University should not appear in any
advertising without prior written consent.
Iowa State University, their respective
officers, agents, or employees, have not
made, and do not hereby make, any
representation, warranty or covenant
with respect to the use of these test
results, nor will they be liable for any
damages, losses, or claims, including
those of an incidental or consequential
nature, arising out of the use of these
test results.
12 Table 1. Iowa evaluation of insecticides and plant-incorporated protectants.
Target Pest and
Test Location
Corn Rootworms
Ames
Crawfordsville
Northeast Iowa
Nashua
European Corn borer
Ames
Corn Earworm
Ames
1
Type of Studies1
Experimental
Unit Size
Table Entries/
Row
#
Numbers Test Length (ft) Reps
1)Pioneer CMRA study
2-4
2)Pioneer dual pyramid
5-7
efficacy and yield study
3)Monsanto corn rootworm 8-10
study
4)Monsanto genuity VT3P 11-12
seed blend study
5)Monsanto smartstax
13-14
features & benefits study
6)Syngenta experimental
15
hybrid efficacy study
7)Syngenta experimental
16
hybrid efficacy study
1)Yield study
17-19
2)Monsanto smartstax RIB 20-21
3)Monsanto smartstax
22-23
features & benefits study
1)Monsanto corn rootworm 24-27
study
28-31
1)Yield study
2)Monsanto corn rootworm 32-35
study
1)Pioneer Optimum
AcreMax II study (SB)
4
6
25
40
6
4
8
40
4
6
100
1
7
200
1
12
20
4
12
20
4
14
6
7
75
100
100
4
1
1
8
40
4
14
8
75
40
4
4
36-39
5
40
4
1)Dow AgroSciences
40-42
smartstax vs HXX &
RR2 traits study (SB)
2)Monsanto smartstax
43-44
features & benefits study
3
20
4
7
200
1
All Studies were conducted on “trap crop corn” ground unless noted otherwise in
parenthesis. (SB) = test conducted on ground that was planted to soybeans in 2010.
13 Table 2. Average root-injury and percent product consistency for the Pioneer CMRA study: Ames, IA.1
NodeTreatment
2
3,4,5
Injury
8,9,10
HXX with Bio-insecticide
HXX
HX1 with Bio-insecticide8,9,10
HX1
0.06a
0.07a
0.16 b
0.27 c
Product
Consistency6,7
94a
97a
80ab
61 b
1
Planted May 11, 2011; node injury evaluated August 15, 2011
HXX = Herculex XTRA hybrid (Pioneer P1395XR); HX1 = Herculex 1 hybrid (Pioneer P1395HR)
3
Chemical and check means based on 36 observations (6 roots/2 rows x 6 replications)
4
Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten
5
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
6
Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis
bacteriophora bioinsecticide (nematodes) solution applied at a rate of 100 nematodes per square inch.
9
Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter
10
Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn
plants and 2 inches deep
2
Table 3. Average stand count for the Pioneer CMRA study: Ames, IA.1
Stand
Treatment2
HX1 with Bio-insecticide5,6,7
HXX with Bio-insecticide5,6,7
HXX
HX1
1
Count3,4
35.75a
35.00ab
34.75ab
34.00 b
Planted May 11, 2011; evaluated June 7 and September 21, 2011
HXX = Herculex XTRA hybrid (Pioneer P1395XR); HX1 = Herculex 1 hybrid (Pioneer P1395HR)
3
Means based on 24 observations (4 row treatment x 20 row-feet/treatment x 6 replications)
4
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
5
Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis
bacteriophora bioinsecticide (nematodes) solution applied at a rate of 100 nematodes per square inch.
6
Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter
7
Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn
plants and 2 inches deep
2
14 Table 4. Average yield for the Pioneer CMRA study: Ames, IA.1
Treatment2
HX1 with Bio-insecticide 6,7,8
HXX with Bio-insecticide 6,7,8
HXX
HX1
Yield3,4,5
202
194
188
183
1
Planted May 11, 2011; Harvested (mechanically) October 13, 2011
HXX = Herculex XTRA hybrid (Pioneer P1395XR); HX1 = Herculex 1 hybrid (Pioneer P1395HR)
3
Means based on 6 observations (4-rows x 20 row-feet/treatment x 6 replications)
4
No significant differences between means (ANOVA, P < 0.05)
5
Yields converted to15% Moisture
6
Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis
bacteriophora bioinsecticide (nematodes) solution applied at a rate of 100 nematodes per square inch.
7
Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter
8
Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn
plants and 2 inches deep
2
Table 5. Average stand count for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA.1
Stand
Treatment
2,3
HXX + MIR604 (Pure stand)
HXX + MIR604 (95% HXX + MIR604; 5% RR2 blended refuge)
HXX + MIR604 (90% HXX + MIR604; 10% RR2 blended refuge)
RR2
+ Force 3G - 0.12/T-band
RR2 (Pure stand) with Poncho 1250/VOTIVO
RR2 (Pure stand) with Cruiser 250
1
Count4,5,6
68.75
67.75
67.75
67.25
67.25
67.25
Planted May 10, 2011; evaluated June 16, 2011
HXX + MIR604 = Dual Pyramided hybrid (Pioneer product TQ38148214); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid
P0413R)
3
HXX = Herculex® XTRA technology. Herculex® Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. .
®Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a
registered trademark of Monsanto Company
Force® 3G is a registered trademark of a Syngenta Group Company
4
Means based on 16 observations (4 row treatment x 38 row-feet/treatment x 4 replications)
5
Insecticide listed as ounces a.i. per 1,000 row-feet; T-band = Insecticide applied at planting time
6
No significant differences between means (ANOVA, P < 0.05)
2
15 Table 6. Average root-injury and percent product consistency for the Pioneer dual pyramid efficacy and yield study
for corn rootworm: Ames, IA,1
NodeTreatment
2.3,4
5,6
Injury
10
RR2 (Pure stand)
+
Force 3G - 0.12/T-band
HXX + MIR604 (90% HXX + MIR604)11
HXX + MIR604 (Pure stand)9
HXX + MIR604 (95% HXX + MIR604)12
RR2 (Pure stand) with Poncho1250/VOTIVO10
RR2 blended refuge (10%)11
RR2 blended refuge (5%)11
RR2 (Pure stand) with Cruiser25010
0.04a
0.05a
0.11ab
0.18 b
0.19 bc
0.30 cd
0.33 cd
0.39
d
Product
Consistency7,8
98a
96a
88ab
73ab
75ab
50ab
57 b
54 b
1
Planted May 10, 2011; node injury evaluated August 15, 2011
HXX + MIR604 = Dual Pyramided hybrid (Pioneer product TQ38148214); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid
P0413R)
3
HXX = Herculex® XTRA technology. Herculex® Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred.
®Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a
registered trademark of Monsanto Company
Force® 3G is a registered trademark of a Syngenta Group Company
4
Insecticide listed as ounces a.i. per 1,000 row-feet; T-band = Insecticide applied at planting time
5
Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten
6
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
7
Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less
8
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
9
For the pure stand treatment of HXX + MIR604, 48 Bt roots were dug (12 roots/2 rows x 4 replications)
10
For the pure stand treatments of RR2, 48 non-Bt roots were dug (12 roots/2 rows x 4 replications)
11
For the RR2 blended refuge treatments (5% & 10%), 24 non-Bt roots were dug (6 roots/2 rows x 4 replications)
12
For the pyramided treatments (90% & 95%), 48 Bt roots were dug (12 roots/2 rows x 4 replications)
2
Table 7. Average yield for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA.1
Treatment2,3,4
RR2 (Pure stand) with Poncho 1250/VOTIVO
RR2
+
Force 3G - 0.12/T-band
HXX + MIR604 (Pure stand)
HXX + MIR604 (95% HXX + MIR604; 5% RR2 blended refuge)
RR2 (Pure stand) with Cruiser 250
HXX + MIR604 (90% HXX + MIR604; 10% RR2 blended refuge)
1
Yield5,6,7
201a
185ab
184ab
184ab
177 bc
166 c
Planted May 10, 2011; machine harvested October 13, 2011
HXX + MIR604 = Dual Pyramided hybrid (Pioneer product TQ38148214); RR2 = Roundup Ready® Corn 2 trait (Pioneer hybrid
P0413R)
3
HXX = Herculex® XTRA technology. Herculex® Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred.
®Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready® Corn 2 gene. ® Roundup Ready is a
registered trademark of Monsanto Company
Force® 3G is a registered trademark of a Syngenta Group Company.
4
Insecticide listed as ounces a.i. per 1,000 row-feet; T-band = Insecticide applied at planting time
5
Means based on 4 observations (2-rows x 38 row-feet/treatment x 4 reps)
6
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
7
Yields converted to15% Moisture
2
16 Table 8. Average root-injury and product consistency for the Monsanto corn rootworm study: Ames, IA.1
NodeTreatment
2
YGVT3 + Force
YGVT3 + Aztec
DeKalb-Iso + Aztec
DeKalb-Iso + Force
YGVT3
Smartstax
DeKalb-Iso
My-HXT
3
Form.
Rate
Placement
3.0G
2.1G
2.1G
3.0G
---------------------
0.12
0.14
0.14
0.12
---------------------
T-band
T-band
T-band
T-band
---------------------
4
Injury
5,6,7
Product
Consistency8,9
0.04a
0.06a
0.10a
0.10a
0.10ab
0.12ab
0.22 bc
0.32 c
95a
95a
85a
85a
95a
80 b
90a
55 b
1
Planted May 10, 2011; evaluated August 15, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications)
6
Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less
9
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 9. Average stand count for the Monsanto corn rootworm study: Ames, IA.
1
Stand
Treatment
2
DeKalb-Iso
My-HXT
DeKalb-Iso + Aztec
Smartstax
DeKalb-Iso + Force
YGVT3
YGVT3 + Aztec
YGVT3 + Force
1
Form.
----------2.1G
-----3.0G
-----2.1G
3.0G
3
Rate
----------0.14
-----0.12
-----0.14
0.12
Placement
----------T-band
-----T-band
-----T-band
T-band
4
Counts5,6
36.25a
36.00ab
35.50abc
35.50abc
35.25abc
35.25abc
34.00 bc
33.50 c
Planted May 10, 2011; evaluated June 7 and September 21, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = Insecticide applied at planting time
5
Means based on 32 observations (4 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluations)
6
No significant differences between means (ANOVA, P < 0.05)
2
17 Table 10. Average yield for the Monsanto corn rootworm study: Ames, IA.1
Treatment
2
My-HXT
DeKalb-Iso
DeKalb-Iso + Force
YGVT3 + Aztec
YGVT3 + Force
YGVT3
DeKalb-Iso + Aztec
Smartstax
3
Form.
Rate
Placement
----------3.0G
2.1G
3.0G
-----2.1G
------
----------0.12
0.14
0.12
-----0.14
------
----------T-band
T-band
T-band
-----T-band
------
4
Bushels/
Acre5,6,7
192
174
168
167
165
163
159
159
1
Planted May 10, 2011; machine harvested October 13, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 4 observations (4-row treatment x 35 row-feet/treatment x 4 replications)
6
No significant differences between means (ANOVA, P < 0.05)
7
Yields converted to 15% Moisture
2
Table 11. Average stand counts for the Monsanto genuity VT3P seed blend efficacy study: Ames, IA.1
Stand
Treatment2
F
Genuity VT3P RIB (100% Bt corn)
Smartstax (95% Bt corn/5% non-Bt corn)
Smartstax (100% Bt corn)
Dekalb-RR2
Genuity VT3P RIB (90% Bt corn/10% non-Bt corn)
Genuity VT3P RIB (80 Bt corn/20% non-Bt corn)
1
Counts3,4
36.25
36.00
35.75
35.25
35.00
34.75
Planted May 10, 2011; evaluated June 7 and September 22, 2011
DeKalb-RR2 = DeKalb brand RR (DKC 61-36); Smartstax (100% Bt corn) = DeKalb brand Smartstax (DKC 61-21); Smartstax
(95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC61-21JRM); Genuity VT3P RIB (90% Bt
corn/10% non-Bt corn) = DeKalb VT3P Refuge in a bag (DKC61-97DKL); Genuity VT3P RIB (100% Bt corn) = DeKalb brand
VT3P Refuge in a bag (DKC61-97WTM); Genuity VT3P RIB (80 Bt corn/20% non-Bt corn) = DeKalb brand VT3P Refuge in a
bag (DKC61-97BPY)
3
Means based on 8 observations (4 rows/ treatment x 17.5 row-feet/treatment x 2 evaluations)
4
No significant differences between means (ANOVA, P < 0.05)
2
18 Table 12. Average yield for the Monsanto genuity VT3P seed blend efficacy study: Ames, IA.1
Treatment
Bushels/
Acre3,4,5
2
Genuity VT3P RIB (80 Bt corn/20% non-Bt corn)
Genuity VT3P RIB (90% Bt corn/10% non-Bt corn)
Genuity VT3P RIB (100% Bt corn)
DeKalb-RR2
Smartstax (100% Bt corn)
Smartstax (95% Bt corn/5% non-Bt corn)
187
173
173
170
149
148
1
Planted May 10, 2011; machine harvested October 12, 2011
DeKalb-RR2 = DeKalb brand RR (DKC 61-36); Smartstax (100% Bt corn) = DeKalb brand Smartstax (DKC 61-21); Smartstax
(95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC61-21JRM); Genuity VT3P RIB (90% Bt
corn/10% non-Bt corn) = DeKalb VT3P Refuge in a bag (DKC61-97DKL); Genuity VT3P RIB (100% Bt corn) = DeKalb brand
VT3P Refuge in a bag (DKC61-97WTM); Genuity VT3P RIB (80 Bt corn/20% non-Bt corn) = DeKalb brand VT3P Refuge in a
bag (DKC61-97BPY)
3
Means based on 1 observation (4-row treatment x 95 row-feet/treatment)
4
No significant differences between means (ANOVA, P < 0.05)
5
Yields converted to 15% Moisture
2
Table 13. Average stand count for the Monsanto genuity smartstax features and benefits study: Ames, IA.1
Stand
Treatment
2
DeKalb-RR2 + Force
Smartstax
Agrisure1 + Force
DeKalb-RR2
Pioneer-HXX
Agrisure2
Pioneer-RR2 + Force
1
Form.
3.0G
-----3.0G
---------------3.0G
3
Rate
0.12
-----0.12
---------------0.12
4
Placement
Counts5,6
T-band
-----T-band
---------------T-band
35.25
34.75
33.50
33.25
33.00
32.50
32.50
Planted May 10, 2011; evaluated June 7 and September 28, 2011
1=
Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure Syngenta Agrisure GT hybrid (N53-W2);
2=
DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure Syngenta Agrisure 3111 hybrid (N53C-3111);
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = Insecticide applied at planting time
5
Means based on 8 observations (4 rows/ treatment x 17.5 row-feet/treatment x 2 evaluations)
6
No significant differences between means (ANOVA, P < 0.05)
2
19 Table 14. Average yield for the Monsanto genuity smartstax features and benefits study: Ames, IA.1
Treatment
2
Pioneer-RR2 + Force
DeKalb-RR2 + Force
Agrisure1 + Force
Agrisure2
DeKalb-RR2
Smartstax
Pioneer-HXX
3
Form.
Rate
3.0G
3.0G
3.0G
---------------------
0.12
0.12
0.12
---------------------
Bushels/
Acre5,6,7
4
Placement
T-band
T-band
T-band
---------------------
162
160
160
155
150
148
143
1
Planted May 10, 2011; machine harvested October 12, 2011
1=
Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure Syngenta Agrisure GT hybrid (N53-W2);
2=
DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure Syngenta Agrisure 3111 hybrid (N53C-3111);
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 4 observations (4-row treatment x 195 row-feet/treatment)
6
No significant differences between means (ANOVA, P < 0.05)
7
Yields converted to 15% Moisture
2
Table 15. Average root-injury and percent product consistency for the Syngenta experimental hybrid efficacy study:
Old Dairy Farm, Ames, IA.1
Treatment2,3
MIR604 + HXRW + HX1 + Bt11
MIR604 + HXRW + HX1 + Bt11 + Force®
95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge + Force®
MIR604 + Bt11 + Force®
90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge + Force®
MIR162 + MIR604 + Bt11 + Force®
95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge
90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge
MIR604 + Bt11
MIR162 + MIR604 + Bt11
Non--Bt + Force®
Non-Bt
1
Rate4 Placement5
-----0.12
0.12
0.12
0.12
0.12
--------------------0.12
------
-----T-band
T-band
T-band
T-band
T-band
--------------------T-band
------
Product
Node
Injury6,7,8 Consistency9,10
0.01a
0.01a
0.01a
0.02a
0.02a
0.02a
0.03a
0.03a
0.05a
0.07a
0.08a
0.20 b
100a
100a
100a
100a
100a
100a
100a
100a
100a
92ab
92ab
79 b
Planted May 18, 2011; evaluated August 18, 2011
MIR604 is the Agrisure® RW trait; MIR162 is the Agrisure Viptera™ trait for above-ground lepidopteran protection
3
Refuge corn does not contain rootworm active Bt but does have Bt11; HXRW = Herculex ® rootworm; Bt11 = Agrisure CB/LL
trait for above-ground lepidopteran protection (e.g., corn borer); HX1 = Herculex I (lepidopteran protection)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
T-band = Insecticide applied at planting time
6
Chemical and check means based on 24 observations (6 roots/2 rows x 4 replications)
7
Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten
8
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
9
Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less
10
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
Force® is a Restricted Use Pesticide.
2
20 Table 16. Average root-injury and percent product consistency for the Syngenta experimental hybrid efficacy study:
South Dairy Farm, Ames, IA.1
Treatment
2,3
4
Rate
MIR604 + HXRW + HX1 + Bt11
95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge
MIR604 + HXRW + HX1 + Bt11 + Force®
95% MIR604 + HX1 + Bt11 + HXRW / 5% refuge + Force®
90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge + Force®
MIR162 + MIR604 + Bt11 + Force®
90% MIR604 + HX1 + Bt11 + HXRW / 10% refuge
MIR604 + Bt11 + Force®
MIR162 + MIR604 + Bt11
MIR604 + Bt11
Non--Bt + Force®
Non-Bt
Placement
----------0.12
0.12
0.12
0.12
-----0.12
----------0.12
------
----------T-band
T-band
T-band
T-band
-----T-band
----------T-band
------
5
Product
Node
6,7,8
Injury
Consistency9,10
0.02a
0.02a
0.03a
0.03a
0.03a
0.04a
0.04a
0.06a
0.06a
0.07a
0.08a
0.18 b
100
100
100
100
100
92
100
96
100
96
92
83
1
Planted May 19, 2011; evaluated August 19, 2011
MIR604 is the Agrisure® RW trait; MIR162 is the Agrisure Viptera™ trait for above-ground lepidopteran protection
3
Refuge corn does not contain rootworm active Bt but does have Bt11; HXRW = Herculex ® rootworm; Bt11 = Agrisure CB/LL
trait for above-ground lepidopteran protection (e.g., corn borer); HX1 = Herculex I (lepidopteran protection)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
T-band = Insecticide applied at planting time
6
Chemical and check means based on 24 observations (6 roots/2 rows x 4 replications)
7
Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten
8
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
9
Product consistency = percentage of times nodal injury was 0.25 (¼ node eaten) or less
10
No significant differences between means (ANOVA, P < 0.05)
Force® is a Restricted Use Pesticide.
2
Table 17. Yield study: Crawfordsville, IA.1
Treatment
2,3
My-HXX + SmartChoice-SB
DeKalb-Smartstax + Aztec
My-HXX + Force
My-Smartstax
My-HXX + Counter-SB
My-HXX
My-Iso + Force
DeKalb-Iso + Aztec
11
My-95%SSTX/5%Non-Bt
DeKalb-Iso
My-Iso
1
4
Form.
Rate
5G
2.1G
3.0G
-----20G
-----3.0G
2.1G
----------------
0.18
0.14
0.12
----0.90
----0.12
0.14
-------------
Placement
In-Furrow
In-Furrow
In-Furrow
----In-Furrow
----In-Furrow
In-Furrow
-------------
5
NodeInjury6,7,8
0.09a
0.18ab
0.24 bc
0.28 bc
0.44 cd
0.51 d
0.56 de
0.77 de
0.99
e
1.54
f
2.27
g
Product
Consistency9,10
90a
75ab
60ab
65ab
45abc
25abc
25 bc
15 bc
8 bc
0 c
0 c
Planted May 5, 2011; evaluated August 11, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Chemical and check means (except My-95%SSTX/5%Non-Bt treatment) based on 20 observations (5 roots/2 rows x 4
replications)
7
Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten
8
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
9
Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less
10
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
11
For the refuge in the bag treatment (My-95%SSTX/5% Non-Bt), mean based on 36 observations (9 roots/2 rows (6 Smartstax (3
adjacent roots and 3 distant roots to a Non-Bt plant) + 3 Non-Bt) x 4 replications)
2
21 Table 18. Yield study: Crawfordsville, IA.1
Treatment
2,3
My-95%SSTX/5%Non-Bt
My-HXX + SmartChoice-SB
My-Smartstax
DeKalb-Iso
DeKalb-Smartstax + Aztec
My-HXX + Force
My-Iso + Force
My-HXX
DeKalb-Iso + Aztec
My-HXX + Counter-SB
My-Iso
4
Form.
Rate
-----5G
----------2.1G
3.0G
3.0G
-----2.1G
20G
------
----0.18
--------0.14
0.12
0.12
----0.14
0.90
-----
Placement
5
----In-Furrow
--------In-Furrow
In-Furrow
In-Furrow
----In-Furrow
In-Furrow
-----
Stand
Count6,7
36.00a
35.25ab
35.25ab
34.50ab
34.50ab
34.50ab
34.25ab
34.00ab
33.75ab
33.50 b
33.50 b
1
Planted May 5, 2011; evaluated June 8 and September 30, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Means based on 16 observations (2-row treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluation dates)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 19. Yield study: Crawfordsville, IA.1
Bushels/
Treatment
2,3
My-HXX + SmartChoice-SB
My-HXX + Force
My-HXX + Counter-SB
My-95%SSTX/5%Non-Bt
My-Smartstax
DeKalb-Iso + Aztec
My-HXX
DeKalb-Iso
DeKalb-Smartstax + Aztec
My-Iso + Force
My-Iso
1
Form.
5G
3.0G
20G
----------2.1G
----------2.1G
3.0G
------
4
Rate
0.18
0.12
0.90
--------0.14
--------0.14
0.12
-----
Placement
In-Furrow
In-Furrow
In-Furrow
--------In-Furrow
--------In-Furrow
In-Furrow
-----
5
Acre6,7,8
155a
152a
142ab
142ab
141ab
139ab
139ab
128ab
119ab
118ab
111 b
Planted May 5, 2011; machine harvested October 7, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Means based on 4 observations (2-row treatment x 69 row-feet/treatment x 4 replications)
7
No significant differences between means (ANOVA, P < 0.05)
8
Yields converted to15% Moisture
2
22 Table 20. Average stand count for the Monsanto smartstax refuge in a bag study: Crawfordsville, IA.1
Treatment
2
DeKalb-RR2 + Aztec
Smartstax RIB (95%Bt/5%non-Bt)
DeKalb-RR2
Smartstax
Pioneer-HXX
Pioneer OAM1
3
Form.
Rate
Placement
2.1G
--------------------------
0.14
--------------------------
T-band
--------------------------
Stand
Counts5,6
4
34.25
34.00
33.75
33.75
33.50
33.00
1
Planted May 5, 2011; evaluated June 8 and September 30, 2011
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer OAM1 = Pioneer Optimum AcreMax (Pioneer P0528XR);
Smartstax (95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC55-08JRM); Pioneer-HXX = Pioneer
brand Herculex XTRA (Pioneer P0528AMI); DeKalb-RR2 = DeKalb brand RR (DKC 55-08)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 8 observations (4 rows/ treatment x 17.5 row-feet/treatment x 2 evaluations)
6
No significant differences between means (ANOVA, P < 0.05)
2
1
Table 21. Average yield for the Monsanto smartstax refuge in a bag study: Crawfordsville, IA.
Treatment
2
Smartstax RIB (95%Bt/5%non-Bt)
Smartstax
Pioneer-HXX
Pioneer OAM1
DeKalb-RR2 + Aztec
DeKalb-RR2
1
3
Form.
Rate
Placement
--------------------2.1G
------
--------------------0.14
------
--------------------T-band
------
4
Bushels/
Acre5,6,7
194
165
155
148
143
142
Planted May 5, 2011; machine harvested October 7, 2011
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer OAM1 = Pioneer Optimum AcreMax (Pioneer P0528XR);
Smartstax (95% Bt corn/5% non-Bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC55-08JRM); Pioneer-HXX = Pioneer
brand Herculex XTRA (Pioneer P0528AMI); DeKalb-RR2 = DeKalb brand RR (DKC 55-08)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 1 observation (4-row treatment x 95 row-feet/treatment)
6
No significant differences between means (ANOVA, P < 0.05)
7
Yields converted to 15% Moisture
2
23 Table 22. Average stand count for the Monsanto genuity smartstax features and benefits study: Crawfordsville, IA.1
Stand
Treatment
2
Smartstax
DeKalb-RR2 + Force
DeKalb-RR2
Pioneer-HXX
Pioneer-RR2 + Force
Agrisure1 + Force
Agrisure2
3
4
Form.
Rate
Placement
Counts5,6
-----3.0G
----------3.0G
3.0G
------
-----0.12
----------0.12
0.12
------
-----T-band
----------T-band
T-band
------
34.25a
33.25ab
33.00ab
32.25ab
32.00ab
31.75ab
30.00 c
1
Planted May 5, 2011; evaluated June 7 and September 28, 2011
1=
Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure Syngenta Agrisure GT hybrid (N53-W2);
2=
DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure Syngenta Agrisure 3111 hybrid (N53C-3111);
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = Insecticide applied at planting time
5
Means based on 8 observations (4 rows/ treatment x 17.5 row-feet/treatment x 2 evaluations)
6
No significant differences between means (ANOVA, P < 0.05)
2
Table 23. Average yield for the Monsanto genuity smartstax features and benefits study: Crawfordsville, IA.1
Treatment
2
Pioneer-HXX
DeKalb-RR2
Smartstax
DeKalb-RR2 + Force
Pioneer-RR2 + Force
Agrisure2
Agrisure1 + Force
1
Form.
------------------3.0G
3.0G
-----3.0G
3
Rate
---------------0.12
0.12
-----0.12
4
Placement
---------------T-band
T-band
-----T-band
Bushels/
Acre5,6,7
160
159
159
153
141
133
114
Planted May 5, 2011; machine harvested October 12, 2011
1=
Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure Syngenta Agrisure GT hybrid (N53-W2);
2=
DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure Syngenta Agrisure 3111 hybrid (N53C-3111);
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 4 observations (4-row treatment x 95 row-feet/treatment)
6
No significant differences between means (ANOVA, P < 0.05)
7
Yields converted to 15% Moisture
2
24 Table 24. Average root-injury and product consistency for the Monsanto corn rootworm study: Northeast Iowa.1
NodeTreatment
2
YGVT3 + Aztec
DeKalb-Iso + Aztec
Smartstax
YGVT3 + Force
DeKalb-Iso + Force
My-HXT
YGVT3
DeKalb-Iso
3
Form.
Rate
2.1G
2.1G
-----3.0G
3.0G
----------------
0.14
0.14
-----0.12
0.12
----------------
4
Placement
T-band
T-band
-----T-band
T-band
----------------
Injury
5,6,7
Product
Consistency8,9
0.30a
0.33a
0.37a
0.57a
1.23 b
1.25 b
1.90 c
2.50 d
65a
45a
55a
35ab
35ab
0 c
5 bc
0 c
1
Planted May 3, 2011; evaluated August 10, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications)
6
Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less
9
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 25. Average stand count for the Monsanto corn rootworm study: Northeast Iowa.1
Stand
Treatment2
DeKalb-Iso + Aztec
YGVT3 + Force
DeKalb-Iso
My-HXT
YGVT3
YGVT3 + Aztec
DeKalb-Iso + Force
Smartstax
1
Form.
Rate3
Placement4
2.1G
3.0G
---------------2.1G
3.0G
------
0.14
0.12
---------------0.14
0.12
------
T-band
T-band
---------------T-band
T-band
------
Counts5,6
36.50
36.25
36.00
35.75
35.25
35.25
35.00
33.50
Planted May 3, 2011; evaluated June 2 and September 16, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = Insecticide applied at planting time
5
Means based on 16 observations (4 row treatment x 17.5 row-feet/treatment x 4 replications)
6
No significant differences between means (ANOVA, P < 0.05)
2
25 Table 26. Average percent lodging for the Monsanto corn rootworm study: Northeast Iowa.1
Treatment
2
YGVT3 + Force
DeKalb-Iso + Aztec
Smartstax
DeKalb-Iso + Force
YGVT3 + Aztec
My-HXT
YGVT3
DeKalb-Iso
3
Form.
Rate
3.0G
2.1G
-----3.0G
2.1G
----------------
0.12
0.14
-----0.12
0.14
----------------
Placement
4
T-band
T-band
-----T-band
T-band
----------------
%
Lodging5,6
1a
3a
6a
7a
11a
17a
57 b
100 c
1
Planted May 3, 2011; evaluated September 16, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 16 observations (4-row treatment x17.5 row-feet/treatment x 4 replications)
6
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 27. Average yield for the Monsanto corn rootworm study: Northeast Iowa.1
Treatment
2
DeKalb-Iso + Aztec
YGVT3 + Force
YGVT3 + Aztec
My-HXT
Smartstax
DeKalb-Iso + Force
DeKalb-Iso
YGVT3
1
3
Form.
Rate
2.1G
3.0G
2.1G
----------3.0G
-----------
0.14
0.12
0.14
----------0.12
-----------
Placement
T-band
T-band
T-band
----------T-band
-----------
4
Bushels/
Acre5,6,7
231
225
224
2218
220
203
195
188
Planted May 3, 2011; machine harvested October 4, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 4 observations (4-row treatment x 35 row-feet/treatment x 4 replications)
6
No significant differences between means (ANOVA, P < 0.05)
7
Yields converted to15% Moisture
8
Means based on 3 observations (4-row treatment x 35 row-feet/treatment x 3 replications) 2
26 Table 28. Yield study: Nashua, IA.1
Treatment2,3
Form.
DeKalb-Smartstax + Aztec
My-HXX + SmartChoice-SB
My-Smartstax
DeKalb-Iso + Aztec
My-HXX + Force
My-HXX + Counter-SB
11
My-95%SSTX/5%Non-Bt
My-HXX
My-Iso + Force
DeKalb-Iso
My-Iso
2.1G
5G
----2.1G
3.0G
20G
--------3.0G
---------
Rate4
0.14
0.18
----0.14
0.12
0.90
--------0.12
---------
Placement5
In-Furrow
In-Furrow
----In-Furrow
In-Furrow
In-Furrow
--------In-Furrow
---------
Product
NodeInjury6,7,8 Consistency9,10
0.00a
0.24 b
0.38 bc
0.64 cd
0.68 cd
0.85
d
0.88 cd
1.03
de
1.51
ef
2.27
fg
2.51
g
100a
75ab
55ab
15 bc
35abc
15 bc
50ab
15 bc
0 c
0 c
0 c
1
Planted May 4, 2011; evaluated August 8, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Chemical and check means (except My-95%SSTX/5%Non-Bt treatment) based on 20 observations (5 roots/2 rows x 4
replications)
7
Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten
8
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
9
Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less
10
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
11
For the refuge in the bag treatment (My-95%SSTX/5%Non-Bt), mean based on 36 observations (9 roots/2 rows (6 Smartstax (3
adjacent roots and 3 distant roots to a Non-Bt plant) + 3 Non-Bt) x 4 replications)
2
Table 29. Yield study: Nashua, IA. 1
Treatment
2,3
My-Iso + Force
DeKalb-Iso + Aztec
My-HXX + SmartChoice-SB
My-Smartstax
My-HXX + Counter-SB
DeKalb-Smartstax + Aztec
My-HXX
My-Iso
My-HXX + Force
DeKalb-Iso
My-95%SSTX/5%Non-Bt
1
4
Form.
Rate
Placement
3.0G
2.1G
5G
----20G
2.1G
--------3.0G
---------
0.12
0.14
0.18
----0.90
0.14
--------0.12
---------
In-Furrow
In-Furrow
In-Furrow
----In-Furrow
In-Furrow
--------In-Furrow
---------
5
Stand
Count6,7
36.00
35.75
35.50
35.50
35.25
35.25
34.50
34.50
34.25
34.25
34.00
Planted May 4, 2011; evaluated June 7 & September 23, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Means based on 16 observations (2-row treatment x17.5 row-feet/treatment x 4 replications x 2 evaluation dates)
7
No significant differences between means (ANOVA, P < 0.05)
2
27 Table 30. Yield study: Nashua, IA.1
Treatment
2,3
My-Iso + Force
My-Smartstax
My-95%SSTX/5%Non-Bt
My-HXX + Force
My-HXX
My-HXX + Counter-SB
My-HXX + SmartChoice-SB
DeKalb-Iso + Aztec
DeKalb-Smartstax + Aztec
My-Iso
DeKalb-Iso
4
Form.
Rate
Placement
3.0G
--------3.0G
----20G
5G
2.1G
2.1G
-----------
0.12
--------0.12
----0.90
0.18
0.14
0.14
-----------
In-Furrow
--------In-Furrow
----In-Furrow
In-Furrow
In-Furrow
In-Furrow
-----------
5
%
Lodging6,7
0a
0a
0a
0a
0a
0a
0a
0a
1a
37 b
61 b
1
Planted May 4, 2011; evaluated September 23, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Means based on 8 observations (2-row treatment x17.5 row-feet/treatment x 4 replications)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 31. Yield study: Nashua, IA.1
Treatment
2,3
DeKalb-Iso + Aztec
My-HXX + SmartChoice-SB
My-Smartstax
My-HXX + Force
My-HXX + Counter-SB
My-HXX
DeKalb-Smartstax + Aztec
My-Iso + Force
My-95%SSTX/5%Non-Bt
My-Iso
DeKalb-Iso
1
4
Form.
Rate
2.1G
5G
----3.0G
20G
-----2.1G
3.0G
----------------
0.14
0.18
-----0.12
0.90
-----0.14
0.12
----------------
5
Placement
In-Furrow
In-Furrow
-----In-Furrow
In-Furrow
-----In-Furrow
In-Furrow
----------------
Bushels/
Acre6,7,8
212a
207a
205a
200a
200a
199a
197a
197a
192a
169 b
166 b
Planted May 4, 2011; machine harvested October 14, 2011
My-Smartstax = Mycogen Smartstax (Mycogen 2K594); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalbSmartstax = DeKalb Smartstax (DKC61-21); DeKalb-Iso = DeKalb brand RR Isoline (DKC 61-72); My-Iso = Mycogen brand RR2
(Mycogen 2K591); My-95%SSTX/5%Non-Bt = Mycogen 95% Smartstax + 5% Non-Bt (Refuge in a Bag) (Mycogen 2K594+
Mycogen 2K591)
3
My-Iso (Mycogen 2K591) is the isoline of My-HXX (Mycogen 2K592)
4
Insecticide listed as ounces a.i. per 1,000 row-ft
5
In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time
6
Means based on 4 observations (2-row treatment x 69 row-feet/treatment x 4 replications)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Yields converted to15% Moisture
2
28 Table 32. Average root-injury and product consistency for the Monsanto corn rootworm study: Nashua, IA.1
NodeTreatment
2
Smartstax
YGVT3 + Aztec
YGVT3 + Force
My-HXT
DeKalb-Iso + Aztec
DeKalb-Iso + Force
YGVT3
DeKalb-Iso
3
Form.
Rate
-----2.1G
3.0G
-----2.1G
3.0G
-----------
-----0.14
0.12
-----0.14
0.12
-----------
Placement
4
5,6,7
Injury
-----T-band
T-band
-----T-band
T-band
-----------
Product
Consistency8,9
0.11a
0.32 b
0.43 bc
0.53 bc
0.62 c
0.64 c
1.41 d
2.13 e
90a
45 b
40 b
15 bc
20 bc
5 c
5 c
0 c
1
Planted May 3, 2011; evaluated August 10, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications)
6
Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less
9
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 33. Average stand count for the Monsanto corn rootworm study: Nashua, IA.
1
Stand
Treatment
2
DeKalb-Iso + Force
My-HXT
YGVT3
DeKalb-Iso
YGVT3 + Aztec
DeKalb-Iso + Aztec
YGVT3 + Force
Smartstax
1
3
Form.
Rate
Placement
3.0G
---------------2.1G
2.1G
3.0G
------
0.12
---------------0.14
0.14
0.12
------
T-band
---------------T-band
T-band
T-band
------
4
Counts5,6
35.75a
35.50a
35.50ab
35.25ab
35.00ab
34.75ab
34.25ab
33.50 b
Planted May 3, 2011; evaluated June 2 and September 16, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = Insecticide applied at planting time
5
Means based on 32 observations (4 row treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluations)
6
No significant differences between means (ANOVA, P < 0.05)
2
29 Table 34. Average percent lodging for the Monsanto corn rootworm study: Nashua, IA.1
Treatment
2
DeKalb-Iso + Aztec
DeKalb-Iso + Force
Smartstax
My-HXT
YGVT3 + Aztec
YGVT3 + Force
YGVT3
DeKalb-Iso
3
4
Form.
Rate
Placement
2.1G
3.0G
----------2.1G
3.0G
-----------
0.14
0.12
----------0.14
0.12
-----------
T-band
T-band
----------T-band
T-band
-----------
%
Lodging5,6
0a
0a
0a
0a
0a
0a
3a
29 b
1
Planted May 3, 2011; evaluated September 16, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92);
YGVT3 = YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on16 observations (4-row treatment x17.5 row-feet/treatment x 4 replications)
6
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 35. Average yield for the Monsanto corn rootworm study: Nashua, IA.1
Treatment
2
My-HXT
YGVT3 + Force
Smartstax
YGVT3
YGVT3 + Aztec
DeKalb-Iso + Force
DeKalb-Iso
DeKalb-Iso + Aztec
1
Form.
Rate
Placement
Bushels/
Acre5,6,7
-----3.0G
----------2.1G
3.0G
-----2.1G
-----0.12
----------0.14
0.12
-----0.14
-----T-band
----------T-band
T-band
-----T-band
231a
210 b
208 b
203 bc
201 bc
201 bc
185 c
182 c
3
4
Planted May 3, 2011; machine harvested October 4, 2011
My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K592); DeKalb-Iso = DeKalb brand RR (DKC 44-92); VGVT3 =
YieldGard VT Triple (DKC46-60); Smartstax = DeKalb brand (DKC46-61)
3
Insecticide listed as ounces a.i. per 1,000 row-feet
4
T-band = insecticide applied at planting time
5
Means based on 4 observations (4-row treatment x 35 row-feet/treatment x 4 replications)
6
No significant differences between means (ANOVA, P < 0.05)
7
Yields converted to 15% Moisture
2
30 Table 36. Average stand count for the Pioneer ECB Optimum AcreMax study: Ames, IA.1
Treatment
2,3
F
HX1 x YGCB (with 5% blended refuge plants)
HX1 x YGCB
HX1 x YGCB (with 10% blended refuge plants)
HX1
RR2
Stand
Count4,5
68.25
68.00
68.00
67.75
67.00
1
Planted May 10, 2011; evaluated June 7, 2011
HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R);
HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R);
3
HX1 = Herculex® 1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty
Link® (LL), Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene.
®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready®
Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.
4
Means based on 16 observations (4 row treatment x 38 row-feet/treatment x 4 replications)
5
No significant differences between means (ANOVA, P < 0.05)
2
Table 37. Average whorl injury for the Pioneer ECB Optimum AcreMax study: Ames, IA.1
ECB whorl
Treatment
2,3
F
HX1 (Pure stand)6
6
HX1 x YGCB (Pure stand)
HX1 x YGCB (pyramid with 5% blended refuge plants)9
RR2 (5% blended refuge)8
HX1 x YGCB (pyramid with 10% blended refuge plants)9
RR2 (Pure stand)7
RR2 (10% blended refuge)8
1
Injury4,5
9.0a
9.0a
9.0a
9.0a
8.9a
8.9a
8.8 b
Planted May 10, 2011; evaluated July 15, 2011
HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R);
HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R);
3
HX1 = Herculex®1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty
Link® (LL), Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene.
®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready®
Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.
4
Means based on the Pioneer 9-1 rating scale for evaluating first generation ECB whorl injury (ECB1LF).
5
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
6
For the pure stand treatments of HX1 x YGCB, 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)
7
For the pure stand treatment of RR2, 48 non-Bt roots were evaluated (12 plants/2 rows x 4 replications)
8
For the RR2 blended refuge treatments (5% & 10%), 24 non-Bt plants were evaluated (6 plants/2 rows x 4 replications)
9
For the pyramided treatments (90% & 95%), 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)
2
31 Table 38. Average tunneling (cm) for the Pioneer ECB Optimum AcreMax study: Ames, IA.1
Average ECB
Tunneling (cm)4
Treatment2,3
HX1 x YGCB (Pure stand)5
HX1 (Pure stand)5
HX1 x YGCB (pyramid with 5% blended refuge plants)8
HX1 x YGCB (pyramid with 10% blended refuge plants)8
RR2 (5% blended refuge)7
RR2 (10% blended refuge)7
RR2 (Pure stand)6
0.00a
0.07a
0.34a
0.43a
3.25 b
5.92 bc
6.88 c
1
Planted May 10, 2011; evaluated October 6 & 7, 2011
HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R);
HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R);
3
HX1 = Herculex® 1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty
Link® (LL), Ignite® and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene.
®YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready®
Corn 2 gene. ® Roundup Ready is a registered trademark of Monsanto Company.
4
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
5
For the pure stand treatments of HX1 x YGCB, 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)
6
For the pure stand treatment of RR2, 48 non-Bt roots were evaluated (12 plants/2 rows x 4 replications)
7
For the RR2 blended refuge treatments (5% & 10%), 24 non-Bt plants were evaluated (6 plants/2 rows x 4 replications)
8
For the pyramided treatments (90% & 95%), 48 Bt plants were evaluated (12 plants/2 rows x 4 replications)
2
1
Table 39. Average yield for the Pioneer ECB Optimum AcreMax study: Ames, IA.
Treatment2,3
HX1 x YGCB
HX1
HX1 x YGCB with 5% blended refuge (RR2)
RR2
HX1 x YGCB with 10% blended refuge (RR2)
1
Yield4,5,6
215
209
209
208
202
Planted May 10, 2011; machine harvested October 13, 2010
HX1 = Herculex® 1 trait (Pioneer® hybrid P0461HR); RR2 = Roundup Ready® Corn 2 trait (Pioneer® hybrid P0461R);
HX1 x YGCB = Pyramid Hybrid (Pioneer P0461YHR/P0461R);
3
HX1 = Herculex® 1 trait. Herculex® 1 Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Ignite®
and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard® Corn Borer gene. ®YieldGard and the
YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready® Corn 2 gene. ®
Roundup Ready is a registered trademark of Monsanto Company.
4
Means based on 4 observations (2-rows x 38 row-feet/treatment x 4 replications)
5
No significant differences between means (ANOVA, P < 0.05)
6
Yields converted to15% Moisture
2
32 Table 40. Stand counts for the Dow AgroSciences Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA.1
Treatment
Stand
Counts3,4
2
RR2
Smartstax
HXX
31.50a
30.50ab
28.50 b
1
Planted May 10, 2011; stand counts evaluated on June 7, 2011
RR2 = Mycogen 2K591; HXX = Mycogen Herculex XTRA 2K592; Smartstax = Mycogen 2K594
3
Stand counts based on 8 observations (2 rows/17.5 feet of row x 4 replications)
4
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 41. Total kernels, damaged kernels, and percent damaged for the Dow AgroSciences Smartstax vs Herculex
1,2
XTRA and RR2 corn study, Ames, IA.
Treatment
3
Smartstax
HXX
RR2
Total
Damaged
4
Kernels/Ear Kernels/Ear4,5
491
519
488
1.23a
3.80 b
4.70 b
%
Damaged/Ear6,7
0.25a
0.73 b
0.96 b
1
Planted May 10, 2011; evaluated August 18, 2011
Ten corn ears per row in rows 2 and 3 of each plot were infested on August 4, 2011 with 5 1st instar CEW larvae
3
RR2 = Mycogen 2K591; HXX = Mycogen Herculex XTRA 2K592; Smartstax = Mycogen 2K594
4
Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications)
5
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
6
Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 42. Average kernel area (cm2) consumed and number of total damaged ears for the Dow AgroSciences
1,2
Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA.
Treatment
Smartstax
HXX
RR2
1
3
Kernel Area
Consumed (cm2)4,5
0.42a
1.18 b
1.46 b
Total
Damaged Ears 6,7
13a
24 b
26 b
Planted May 10, 2011; evaluated August 18, 2011
Ten corn ears per row in rows 2 and 3 of each plot were infested on August 4, 2011 with 5 1st instar CEW larvae
3
RR2 = Mycogen 2K591; HXX = Mycogen Herculex XTRA 2K592; Smartstax = Mycogen 2K594
4
Kernel area consumed based on 40 observations (10 ears/treatment x 4 replications)
5
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
6
Total damaged ears based on 40 observations (10 ears/treatment x 4 replications)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
33 Table 43. Total kernels, damaged kernels, and percent damaged for the Monsanto genuity smartstax features and
benefits study, Ames, IA.1,2
Treatment
3
2
Agrisure
Smartstax
Pioneer-HXX
DeKalb-RR2
DeKalb-RR2 + Force
Agrisure1 + Force
Pioneer-RR2 + Force
4
5
Form. Rate Placement
--------------------2.1G
2.1G
2.1G
--------------------0.12
0.12
0.12
--------------------T-band
T-band
T-band
Total
Kernels/Ear6
442
411
461
447
318
404
435
Damaged
Kernels/Ear6,7
0.0a
0.0a
4.7 b
9.2 b
11.8 b
12.6 b
15.2 b
Percent
Damaged/Ear8,9
0.0a
0.0a
1.0 b
2.1 b
3.7 b
3.1 b
3.5 b
1
Planted May 10, 2011; evaluated August 24, 2011
Ten corn ears per row in rows 2 and 7 of each plot were infested on August 10, 2011 with 5 2nd instar CEW larvae
1=
3
Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure Syngenta Agrisure GT hybrid (N53-W2);
2=
DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure Syngenta Agrisure 3111 hybrid (N53C-3111);
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
T-band = Insecticide applied at planting time
6
Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Mean total kernels, damaged kernels counts and % damaged based on 40 observations (10 ears/treatment x 4 replications)
9
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
Table 44. Kernel area (cm2) consumed and number of total damaged ears for the Monsanto genuity smartstax
1,2
features and benefits study, Ames, IA.
Treatment
2
3
Agrisure
Smartstax
Pioneer-HXX
DeKalb-RR2
DeKalb-RR2 + Force
Agrisure1 + Force
Pioneer-RR2 + Force
1
4
Form.
Rate
--------------------2.1G
2.1G
2.1G
--------------------0.12
0.12
0.12
5
Placement
----------------------T-band
T-band
T-band
Kernel Area
Consumed (cm2)6,7
0.00a
0.00a
1.34 b
3.16 b
4.97 b
5.05 b
5.50 b
Total
Damaged Ears
0a
0a
8b
9b
7b
7b
10 b
Planted May 10, 2011; evaluated August 24, 2011
Ten corn ears per row in rows 2 and 7 of each plot were infested on August 10, 2011 with 5 2nd instar CEW larvae
1=
3
Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 35F34PDR); Agrisure Syngenta Agrisure GT hybrid (N53-W2);
2=
DeKalb-RR2 = DeKalb brand RR (DKC 55-08); Agrisure Syngenta Agrisure 3111 hybrid (N53C-3111);
Smartstax = DeKalb brand Smartstax (DKC 55-09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 35F37)
4
Insecticide listed as ounces a.i. per 1,000 row-feet
5
T-band = Insecticide applied at planting time
6
Kernel area consumed based on 40 observations (10 ears/treatment x 4 replications)
7
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
8
Total damaged ears based on 40 observations (10 ears/treatment x 4 replications)
9
Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)
2
8,9
34 APPENDIX I
Agronomic Information 35 2011
Field History Data
Ames, IA
Corn rootworm studies
(following trap crop)
Crawfordsville, IA
Corn rootworm studies
(following trap crop)
No Insecticide (trap crop)
No Insecticide (soybeans)
Insecticide Test Plot
No Insecticide (trap crop)
No Insecticide (trap crop)
Insecticide Test Plot
No Insecticide (trap crop)
Insecticide Test Plot
Tillage
Fall-disk ripper;
Spring-field cultivation (2X)
Fall-chiseled;
Spring-field cultivated
Variety
Pioneer P1395XR, Pioneer
TQ38148214, Pioneer P1395HR,
Pioneer P0413R, DKC46-60,
DKC44-92, DKC46-61, Mycogen
2K592, DKC61-36, DKC61-21,
DKC62-97DKL, DKC61-21JRM,
DKC61-97WTM, DKC62-97BPY
DKC61-21 & DKC61-72, Mycogen
2K591, Mycogen 2K592, Mycogen
2K594, Pioneer 35F34PDR, Agrisure
N53C-3111, DKC55-09, DKC55-08,
Agrisure N53-W2, Pioneer 35F37,
DKC55-08
Planting Date(s)
Planting Rate
May 10
35,600 seeds/A
May 5
35,600 seeds/A
Herbicide1
6 oz Balance + 32 oz Glystar-May 2;
3 oz Capreno-June 18
2.5 qt Harness Extra +
3 oz Balance Flexx-May 7;
22 oz Roundup PowerMax +
1.25 qt Infantry 4L +
2.5 lbs AMS-June 7
N
--136
N
--250
Insecticide History
2010
2009
2008
2007
2
Fertilizer
Fall Applied
Preplant
P
-----
K
-----
P
-----
K
-----
Dates
Stand Count
Root Digging
Lodging
Harvest
June 7, September 22
August 10,11,12
September 21,22
October 12,13
June 8, September 30
August 9
September 30
October 7
Soil Type
Clay Loam
Silty Clay Loam
Soil Organic Matter %
3.7
5.1
Soil pH
5.6
4.9
1
2
Expressed as formulation per acre.
Expressed as pounds per acre. Spring-Preplant: 136 lbs actual nitrogen applied as urea on May 2 (Ames);
Spring-Preplant 250 lbs actual nitrogen applied as anhydrous ammonia (82.5-0-0) on April 2 (Crawfordsville).
36 2011
Field History Data
Nashua, IA
Corn rootworm studies
(following trap crop)
Nashua, IA
Monsanto corn rootworm study
(following continuous corn)
No Insecticide (trap crop)
Insecticide Test Plot
No Insecticide (trap crop)
Insecticide Test Plot
VT3 Hybrid Test Plot (Aztec)
VT3 Hybrid Test Plot
VT3 Hybrid Test Plot
YIELDGARD + Hybrid Test Plot
Tillage
Fall-chopped stalks & chisel;
Spring-field cultivated
Fall-chopped stalks & chisel; Springfield cultivated
Variety
DKC61-21 & DKC61-72,
Mycogen 2K591, Mycogen
2K592, Mycogen 2K594,
DKC46-60, DKC44-92 &
DKC46-61; Mycogen 2K592
Planting Date(s)
Planting Rate
May 4
35,600 seeds/A
May 4
35,600 seeds/A
Herbicides1
2.3 qt Harness Extra-May 9;
2 pt Marksman-June 1
2.3 qt Harness Extra-May 9;
2 pt Marksman-June 1
Fertilizer2
Fall Applied
Preplant
N
P
--- --200 ---
N
P
--- --200 ---
Dates
Stand Count
Root Digging
Lodging
Harvest
June 7 & September 23
August 1
September 23
October 14
June 7 & September 23
August 1
September 23
October 14
Soil Type
Loam
Loam
Soil Organic Matter %
3.9
3.9
Soil pH
7.6
7.6
Insecticide History
2010
2009
2008
2007
1
2
K
-----
K
-----
Expressed as formulation per acre.
Expressed as pounds per acre. Spring-preplant 200 lbs actual nitrogen applied as anhydrous ammonia (82.5-00) on April 3 (Nashua).
37 2011
Field History Data
Northeast Iowa
Monsanto corn rootworm study
(following continuous corn)
Ames, IA
Pioneer ECB OAM II study
(following soybeans)
Corn
Corn (Insecticide applied)
Corn (Insecticide applied)
Corn (Insecticide applied)
No insecticide (SB’s)
No insecticide (SB’s)
No insecticide (SB’s)
Soybeans (with Warrior)
Tillage
Fall-chopped stalks & chisel;
Spring-field cultivated
Spring-field cultivation (2X)
Variety
DKC46-60, DKC44-92 & DKC46- Pioneer P0461HR, Pioneer
P0461YHR, Pioneer P0461R
61; Mycogen 2K592
Planting Date
Planting Rate
May 4
35,600 seeds/A
Herbicides1
2.5 pts G-Max LiteTM +
2.5 pts Harness-May 2;
1 qt Accomplish-April 6;
3 oz Capreno-June 8.
22 oz Roundup PowerMax +
1 oz Status + 2 oz Radiate-June 6
4 oz Headline-June 6;
None
9 oz Headline Amp-July 20.
Insecticide History
2010
2009
2008
2007
Fungicides1
Fertilizer2
Fall Applied
Preplant
Dates
Stand Count
Root Digging
ECB Scoring
Stalk Rating
Lodging
Harvest
N
170
75
P
-----
K
-----
June 2 & September 16
August 5
- N/A
N/A
September 16
October 4
May 11
35,600 seeds/A
N
--136
Clay loam
Loam
Soil Organic Matter
3.5
4.1
Soil pH
6.5
5.8
2
K
90
---
June 7, 15 & 30, September 21
N/A
July 15
October 6,7
September 21
October 12
Soil Type
1
P
60
---
Expressed as formulation per acre.
Expressed as pounds per acre. Spring-preplant 136 lbs actual nitrogen (urea) applied on May 2 (Ames). Fall2010 applied 60 lbs of Phosphorus and 90 lbs of potassium (Ames). Spring-preplant 75 lbs applied as 28%
Nitrogen fertilizer on April 6 (Northeast Iowa). Fall-applied 170 lbs actual nitrogen applied as anhydrous
ammonia (82.5-0- 0) in November 2010 (Northeast Iowa).
38 2011
Field History Data
Ames, IA-Old Dairy Farm
Syngenta efficacy study
(following corn)
Ames, IA-South Dairy Farm
Syngenta efficacy study
(following corn)
Corn
Soybeans
Corn
Soybeans
Corn
Corn
Soybeans (Warrior)
Corn
Tillage
Fall-Chisel plow
Spring-field cultivation (2X)
Fall-Disk ripper
Spring-field cultivation (2X)
Variety
Agrisure GT/CB/LL, Agrisure
3000GT, Agrisure Viptera 3111,
Agrisure Viptera 3122, Agrisure
3122 Viptera EZ Refuge, Agrisure
3122 Viptera EZ Refuge2
Agrisure GT/CB/LL, Agrisure
3000GT, Agrisure Viptera 3111,
Agrisure Viptera 3122, Agrisure
3122 Viptera EZ Refuge, Agrisure
3122 Viptera EZ Refuge2
Planting Date
Planting Rate
May 18
32,000 seeds/A
May 19
32,000 seeds/A
Herbicides1
28 oz Dual II Magnum-May 11;
3 oz Laudis-June 16.
28 oz Dual II Magnum-May 19;
6 oz Status-June 24.
Fertilizer2
Fall Applied
Preplant
N
--210
N
--210
Dates
Stand Count
Root Digging
Lodging
Harvest
June 8 & October 19
August 17
October 19
October 19
June 8 & October 20
August 17
October 20
October 20
Soil Type
Loam
Loam
Soil Organic Matter %
6.5
3.0
Soil pH
6.5
6.5
Insecticide History
2010
2009
2008
2007
1
2
P
-----
K
-----
P
-----
K
-----
Expressed as formulation per acre.
Expressed as pounds per acre. Spring-preplant 210 lbs actual nitrogen (urea) applied on May 10 at Old Dairy
Farm (Ames). Spring-preplant 210 lbs actual nitrogen (urea) applied on May 16 at South Dairy Farm (Ames).
39 2011
Field History Data
Ames, IA
Dow AgroSciences CEW study
(following soybeans)
Ames, IA
Monsanto features & benefits study
(following trap crop)
Insecticide History
2010
2009
2008
2007
No insecticide (SB’s)
No insecticide (corn)
No insecticide (SB’s)
No insecticide (corn)
No Insecticide (trap crop)
No Insecticide (soybeans)
Insecticide Test Plot
No Insecticide (trap crop)
Tillage
Spring-field cultivation (2X)
Fall-disk ripper;
Spring-field cultivation (2X)
Variety
Mycogen 2K591, Mycogen
2K592, Mycogen 2K594
Pioneer 35F34PDR, Agrisure N53C3111, DKC55-09, DKC55-08, Agrisure
N53-W2, Pioneer 35F37, DKC55-08
Planting Date
Planting Rate
May 10
35,600 seeds/A
May 10
35,600 seeds/A
Herbicides1
2.5 pts Harness-May 2;
3 oz Capreno-June 8.
6 oz Balance + 32 oz Glystar-May 2;
3 oz Capreno-June 18
Fertilizer2
Fall Applied
Preplant
N
--136
N
--136
Dates
Stand Count
CEW Infestations
CEW Ear
assessments
June 7
August 4
August 18
June 7 and September 22
August 10
August 24
Soil Type
Loam
Clay Loam
Soil Organic Matter
4.1
3.7
Soil pH
5.8
5.6
1
2
P
60
---
K
90
---
P
-----
K
-----
Expressed as formulation per acre.
Expressed as pounds per acre. Spring-preplant 136 lbs actual nitrogen (urea) applied on May 2. (Ames);
Fall-2010 applied 60 lbs of Phosphorus and 90 lbs of potassium. (Ames-Dow Agro Sciences study only).
40 APPENDIX II
Weather Data 41 Ames1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
May
Temp (F) Rainfall
High Low Inches
58
54
59
53
56
58
60
76
77
99
82
67
61
54
64
66
57
72
75
63
66
74
76
78
61
64
57
62
62
90
79
33
27
26
43
48
46
52
50
57
59
64
60
54
51
42
36
48
39
48
59
61
57
55
55
49
44
54
55
57
67
58
58.6
61.3
-2.70
0.01
0.28
0.23
0.23
0.11
0.31
0.90
0.21
0.01
0.43
0.90
0.05
June
Temp (F) Rainfall
High Low Inches
81
82
77
74
75
99
100
87
66
64
66
68
72
73
75
85
73
71
83
78
72
69
64
67
66
70
69
70
73
79
49
65
68
68
66
70
73
60
54
62
61
52
54
60
57
54
66
68
62
62
62
60
55
60
62
63
64
63
65
70
0.02
1.56
0.42
0.04
0.31
0.12
0.13
0.47
0.21
0.03
0.13
0.98
0.05
3.72
4.19
-0.47
68.5
70.4
-1.90
Weather station located 5 miles WSW of test site.
4.42
4.76
-0.34
July
Temp (F) Rainfall
High Low Inches
80
77
75
76
80
84
86
78
78
89
84
72
77
79
78
80
92
94
93
92
81
80
79
82
86
88
87
86
80
80
88
73
71
70
68
62
62
63
70
73
72
68
60
57
62
73
75
77
74
76
74
76
75
75
61
56
69
75
70
74
73
70
76.1
73.8
2.30
0.24
0.08
0.50
0.26
0.02
0.03
0.43
0.26
0.08
0.02
0.48
2.40
4.43
-2.03
42 Ames1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
August
Temp (F) Rainfall
High Low Inches
91
90
85
76
78
87
82
80
79
76
80
82
76
83
83
77
83
84
84
79
81
84
90
85
83
80
82
77
80
66
86
71
74
66
66
70
70
65
64
60
57
58
63
63
59
59
63
66
61
60
62
62
63
70
60
56
60
61
57
56
62
61
72.2
71.5
0.07
0.98
0.15
0.23
0.10
0.07
0.03
1.56
4.55
-2.99
September
Temp (F) Rainfall
High Low Inches
94
89
73
70
69
71
72
79
80
81
85
88
72
60
59
53
58
60
76
75
65
61
66
65
66
66
68
82
72
67
71
67
57
52
46
47
48
50
59
55
56
58
53
42
34
45
49
53
54
51
48
41
39
38
39
49
52
54
54
43
60.8
64.2
-3.40
Weather station located 5 miles WSW of test site.
0.01
0.05
0.01
0.04
0.62
0.01
0.03
0.77
3.00
-2.23
October
Temp (F) Rainfall
High Low Inches
65
75
78
83
82
80
82
82
79
74
76
67
68
62
67
61
57
49
48
54
65
65
74
73
76
53
55
56
57
55
57
35
35
42
48
51
50
60
61
54
52
47
54
45
44
40
39
33
34
33
31
27
42
39
38
45
40
33
30
27
37
28
54.1
52.2
1.9
0.01
0.51
0.52
2.37
-1.85
43 Crawfordsville1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
May
Temp (F) Rainfall
High Low Inches
61
59
54
64
63
72
79
78
85
94
89
86
66
53
65
64
68
72
77
82
82
82
79
84
66
66
65
72
77
90
84
40
34
30
26
44
40
52
48
59
62
65
59
53
48
42
35
34
39
48
57
62
57
62
58
50
46
42
53
53
62
62
61.3
61.5
-0.02
0.05
0.10
0.69
0.97
0.01
0.39
0.38
0.23
0.77
1.19
0.87
5.65
5.15
0.50
Weather station located at test site.
June
Temp (F) Rainfall
High Low Inches
85
71
92
88
87
95
94
92
70
72
69
72
74
75
78
85
82
78
85
82
83
69
68
75
70
82
83
81
84
94
53
61
65
66
63
68
71
69
57
53
55
51
55
57
61
56
62
63
65
66
65
62
60
57
56
58
62
56
56
65
70.5
70.8
-0.03
0.03
0.40
0.33
1.34
0.04
1.22
0.09
1.04
2.05
0.37
0.17
0.35
0.74
8.17
5.37
2.80
July
Temp (F) Rainfall
High Low Inches
95
89
83
88
86
90
84
91
90
93
88
85
80
85
89
90
94
96
99
97
89
86
84
84
86
90
90
86
85
87
90
76
67
64
61
60
63
65
63
63
69
70
65
64
60
67
71
72
78
78
76
71
68
70
68
63
68
73
70
67
65
65
78.2
73.7
4.5
0.03
0.67
0.31
0.04
0.26
1.31
3.33
-2.02
44 Crawfordsville1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
August
Temp (F) Rainfall
High Low Inches
91
92
86
81
82
86
85
79
79
76
81
82
76
81
81
79
84
84
86
80
81
84
87
85
83
81
82
76
80
69
85
68
74
64
62
68
69
65
62
60
54
51
58
61
56
55
58
59
55
57
62
56
55
67
61
56
55
60
55
53
59
61
70.9
72.2
-1.3
0.31
September
Temp (F) Rainfall
High Low Inches
93
88
74
70
69
70
72
79
79
81
85
87
72
61
59
53
62
61
75
77
65
62
63
63
65
61
66
71
71
66
69
70
59
52
44
42
43
49
53
52
52
56
50
39
34
43
46
56
47
45
48
40
36
34
39
48
50
49
49
42
1.19
0.06
0.27
0.25
0.73
1.04
5.01
-3.97
Weather station located at test site.
59.4
64.8
5.4
1.77
3.21
-1.44
October
Temp (F) Rainfall
High Low Inches
63
73
80
82
84
84
86
85
83
80
77
68
69
60
66
60
55
52
35
36
61
68
78
70
78
52
56
59
58
55
54
32
31
37
42
44
45
52
53
48
45
53
50
49
46
40
40
33
39
33
32
28
33
36
37
50
42
30
25
27
34
31
52.9
51.9
1.0
0.23
0.30
0.06
0.59
3.29
-2.70
45 Northeast Iowa1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
May
Temp (F) Rainfall
High Low Inches
68
58
54
51
62
64
72
66
73
71
91
87
85
57
50
65
62
66
65
74
77
81
77
78
80
54
63
62
70
74
87
38
28
29
24
29
37
44
40
56
59
64
61
54
45
45
34
30
35
44
53
59
57
60
51
49
45
38
44
47
53
69
57.5
58.0
-0.05
T
T
T
0.31
0.02
0.01
0.03
0.19
0.20
T
T
T
0.24
T
0.65
0.19
0.04
0.28
0.01
2.17
4.32
-2.15
June
Temp (F) Rainfall
High Low Inches
81
82
68
90
87
88
96
94
93
65
68
69
71
72
75
74
83
83
78
84
79
81
67
64
73
69
79
81
79
82
51
55
58
67
59
65
69
73
60
52
55
47
51
57
59
56
59
63
65
67
64
63
58
56
56
54
61
55
53
61
68.7
67.9
0.08
0.01
0.08
T
0.08
1.68
T
0.02
0.01
T
1.14
0.33
0.03
0.05
0.01
0.39
0.23
0.07
4.16
5.17
-1.01
July
Temp (F) Rainfall
High Low Inches
92
95
86
80
86
84
88
84
88
87
89
85
80
78
78
84
89
93
95
97
95
85
86
84
86
85
88
90
82
87
87
70
70
58
55
56
62
64
61
63
68
67
64
59
57
59
69
71
77
77
73
76
66
67
70
61
63
72
70
68
65
64
76.4
71.7
4.7
T
T
T
T
1.31
0.04
0.12
T
0.18
0.48
0.65
3.72
0.73
T
2.37
0.49
10.09
4.91
5.18
Closest weather station located in Manchester, Iowa, approximately 20 miles West
Northwest.
46 Northeast Iowa1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
August
Temp (F) Rainfall
High Low Inches
90
90
92
85
82
83
85
85
75
78
76
80
81
75
80
81
82
83
83
87
81
79
84
83
84
81
82
79
76
80
68
69
71
71
61
65
67
68
61
62
53
49
50
61
55
53
55
58
51
55
57
56
53
53
66
54
53
54
53
53
54
59
69.8
69.5
0.03
T
0.02
0.32
0.01
0.08
0.05
0.01
0.47
0.53
0.03
September
Temp (F) Rainfall
High Low Inches
84
92
87
74
68
66
68
71
77
76
79
83
85
70
59
58
51
64
59
73
79
64
59
57
61
62
59
66
64
69
62
71
67
52
46
40
41
41
49
50
51
58
53
44
32
33
41
46
56
44
46
47
39
35
39
47
47
47
46
46
0.08
0.27
0.35
T
0.07
0.14
T
T
0.08
0.10
0.10
0.50
0.19
0.02
0.01
1.28
2.80
4.84
-2.04
58.3
60.9
-2.6
1.91
3.30
-1.39
October
Temp (F) Rainfall
High Low Inches
63
63
72
78
79
81
80
83
83
79
77
74
64
66
59
62
60
55
48
48
53
59
65
68
67
73
49
53
58
54
52
30
29
29
37
40
43
44
57
48
44
44
49
50
45
41
41
35
35
35
35
27
27
29
30
31
42
38
24
23
23
34
51.0
48.9
2.1
0.13
T
0.76
T
0.11
0.11
0.02
T
T
T
0.14
1.27
2.85
-1.58
Closest weather station located in Manchester, Iowa, approximately 20 miles West
Northwest.
47 Nashua1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
May
Temp (F) Rainfall
High Low Inches
57
49
56
63
66
72
69
74
68
93
81
82
57
50
63
64
66
70
73
75
77
75
78
77
57
64
60
74
65
89
81
35
28
32
29
46
38
47
44
54
61
63
51
50
43
43
38
40
39
51
58
59
57
56
54
49
45
44
52
52
64
59
58.5
59.2
-0.07
T
T
0.09
0.18
0.05
0.14
0.31
0.30
0.37
0.44
0.18
0.05
1.22
0.04
0.01
June
Temp (F) Rainfall
High Low Inches
82
72
95
87
89
97
97
91
66
61
69
67
72
73
74
84
85
81
83
77
78
72
63
74
72
81
78
79
82
94
50
60
64
67
62
69
69
63
55
53
52
49
53
58
59
57
62
63
66
66
63
61
57
52
57
56
58
52
57
66
0.13
0.01
0.74
0.43
T
0.20
1.43
0.24
0.77
0.65
0.08
T
0.07
0.01
3.84
4.49
-0.65
Weather station located at test site.
69.2
68.5
0.07
4.75
5.17
-0.42
July
Temp (F) Rainfall
High Low Inches
94
85
79
85
80
85
86
89
86
89
85
74
78
79
87
89
92
94
96
93
80
82
86
83
85
87
87
82
87
87
89
71
64
61
56
61
63
63
61
65
70
69
60
56
59
68
70
76
76
77
74
68
69
71
64
58
70
74
69
66
65
67
76.2
71.9
4.3
0.02
T
T
0.03
0.20
0.08
0.08
1.67
0.07
0.31
0.64
T
0.38
3.48
4.81
-1.33
48 Nashua1
Rainfall and Temperature 2011
Day
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Mean/Total
Normal
D.F.N.
1
August
Temp (F) Rainfall
High Low Inches
90
90
84
81
83
84
83
79
77
76
81
81
74
81
81
81
80
84
86
77
80
84
86
80
83
82
79
74
80
65
84
70
73
63
61
68
66
64
62
55
52
50
60
60
56
57
58
58
55
57
60
54
55
66
57
55
58
57
54
52
61
64
70.1
69.7
0.04
T
0.13
0.20
1.95
T
0.31
1.43
0.58
4.60
4.31
0.29
Weather station located at test site.
September
Temp (F) Rainfall
High Low Inches
92
82
75
67
68
69
72
79
79
81
86
86
69
58
58
53
62
59
73
74
61
58
61
60
63
59
66
74
69
64
69
68
54
48
43
43
43
49
55
51
53
57
50
37
32
45
48
55
49
49
50
46
41
36
42
50
51
52
51
40
59.0
62.0
-3.0
0.30
0.84
0.01
0.67
0.04
T
0.46
T
2.32
3.11
-0.79
October
Temp (F) Rainfall
High Low Inches
63
74
79
80
83
80
83
82
80
76
76
66
63
58
61
60
58
45
49
54
61
63
69
71
74
51
53
55
55
52
52
34
34
41
45
51
50
61
61
51
50
53
54
46
44
38
36
33
33
39
30
26
37
36
34
44
41
33
28
23
37
33
52.9
49.2
3.7
0.17
1.26
0.02
0.07
1.52
2.63
-1.11
49 APPENDIX III
Materials Tested 50 Materials Tested in 2011 Iowa State University Efficacy Studies
Common/code name
Formulation
Chemical name
Aztec
2.1G
tebupirimphos & cyfluthrin
Counter
20G
Commercially applied
seed trt (0.25 mg/sd)
Commercially applied
seed trt (0.25 mg/sd)
RR2 +
commercially applied
Poncho 500/VOTIVO
Transgenic seedcorn
(YGRW+YGCB+RR2) +
commercially applied
Poncho 500/VOTIVO
Transgenic seedcorn
(CRW/RR2(VT)) +BT
(VT)+CRW
(HXRW)+BT (HX1)
commercially applied
Poncho 500/VOTIVO
RR2 +
commercially applied
Poncho 500/VOTIVO
Transgenic seedcorn
(CRW/RR2(VT)) +BT
(VT)+CRW
(HXRW)+BT (HX1)
commercially applied
Poncho 500/VOTIVO
Transgenic seedcorn
(CRW/RR2(VT)) +BT
(VT)+CRW
(HXRW)+BT (HX1)
commercially applied
Poncho 500/VOTIVO
Transgenic seedcorn
(CRW/RR2(VT)) +BT
(VT)+CRW
(HXRW)+BT (HX1)
commercially applied
Poncho 500/VOTIVO
RR2 +
commercially applied
Poncho 500/VOTIVO
DKC61-72 (RR2) ‘’true
isoline seed” of
DKC61-69 +
commercially applied
Poncho 500/VOTIVO
terbufos
Cruiser
Cruiser Extreme 250
DKC44-92
DKC46-60 (YieldGard
VT Triple with
commercial seed trt)
DKC46-61 (Smartstax
with commercial seed trt)
DKC55-08
DKC55-09 (Smartstax
with commercial seed trt)
DKC61-21 (Smartstax
with commercial seed trt)
DKC61-21JRM
(Smartstax RIB
(95%Bt/5%non-Bt) with
commercial seed trt)
DKC61-36
DKC61-72
DKC61-97DKL VT3P
RIB (95%Bt/5%non-Bt)
with commercial seed trt
Transgenic seedcorn
(YGRW+YGCB+RR2)+
commercially applied
Poncho 500/VOTIVO
thiamethoxam
thiamethoxam+ three fungicides (Maxim
XL+Apron XL and Dynasty)
Roundup Ready 2 Corn + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Cry3Bb1 (MON 863) + Cry1Ab (MON810) +
Roundup Ready Corn 2 + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Cry3Bb1 & RR2 (MON 88017) +
Cry1A.105&Cry2Ab2(MON89034)
+Cry34Ab1&Cry35Ab1(DAS59122)
+Cry1F&LL(TC1507) + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.25 mg/seed) + Bacillus firmus I-1582
Roundup Ready 2 Corn + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Cry3Bb1 & RR2 (MON 88017) +
Cry1A.105&Cry2Ab2(MON89034)
+Cry34Ab1&Cry35Ab1(DAS59122)
+Cry1F&LL(TC1507) + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Cry3Bb1 & RR2 (MON 88017) +
Cry1A.105&Cry2Ab2(MON89034)
+Cry34Ab1&Cry35Ab1(DAS59122)
+Cry1F&LL(TC1507) + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Cry3Bb1 & RR2 (MON 88017) +
Cry1A.105&Cry2Ab2(MON89034)
+Cry34Ab1&Cry35Ab1(DAS59122)
+Cry1F&LL(TC1507) + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Roundup Ready 2 Corn + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Roundup Ready 2 Corn + fludioxonil &
mefenoxam fungicides + clothianidin seed trt
(0.50 mg/seed) + Bacillus firmus I-1582
Cry3Bb1 (MON 863) + Cry1A.105/Cry2Ab2
(MON89034) + Roundup Ready 2 Corn +
fludioxonil & mefenoxam fungicides +
clothianidin seed trt (0.50 mg/seed) +
Bacillus firmus I-1582
Company
AMVAC Chem.
Corp.
AMVAC Chem.
Corp.
Syngenta
Syngenta
Monsanto
Monsanto
Monsanto
Monsanto
Monsanto
Monsanto
Monsanto
Monsanto
Monsanto
Monsanto
51 Materials Tested for 2011 (Continued)
Common/code name
DKC61-97WTM VT3P
RIB (100%Bt) with
commercial seed trt
Formulation
Transgenic seedcorn
(YGRW+YGCB) +
commercially applied
Poncho 500/VOTIVO
DKC61-97BPY VT3P
RIB (80%Bt/20%non-Bt)
with commercial seed trt
Transgenic seedcorn
(YGRW+YGCB+RR2)+
commercially applied
Poncho 500/VOTIVO
Force
Mycogen 2K591
Mycogen 2K592
(Herculex/RR) with
Cruiser Extreme
250 seed trt
Mycogen 2K594
(Smartstax) with Cruiser
Extreme 250 seed trt
Mycogen 2K594+2K591
(RIB) with Cruiser
Extreme 250 seed trt
Pioneer P0413R (RR2)
with Poncho
1250/VOTIVO seed
treatment
Pioneer P0413R (RR2)
with Cruiser 250 seed
treatment
Pioneer TQ38148214
(pyramid) with Cruiser
250 seed treatment
Pioneer P1395HR (HX1)
with Cruiser 250 seed
treatment
Pioneer P1395XR
(Herculex XTRA) with
Cruiser 250 seed
treatment
Pioneer P0461HR (HX1)
with Cruiser 250 seed
treatment
3G
Mycogen 2K591 “true
isoline seed” of
Mycogen 2K592
Dow Background
Dow Background
Dow Background
Pioneer Background
Pioneer Background
Pioneer Background
Pioneer P1395HR “near
isoline seed” of Pioneer
P1395XR
Pioneer Background
Pioneer P0461HR “near
isoline seed” of Pioneer
P0461R
Chemical name
Cry3Bb1 (MON 863) + Cry1A.105/Cry2Ab2
(MON89034) + fludioxonil & mefenoxam
fungicides + clothianidin seed trt (0.50
mg/seed) + Bacillus firmus I-1582
Cry3Bb1 (MON 863) + Cry1A.105/Cry2Ab2
(MON89034) + Roundup Ready 2 Corn +
fludioxonil & mefenoxam fungicides +
clothianidin seed trt (0.50 mg/seed) +
Bacillus firmus I-1582
Monsanto
tefluthrin
Syngenta Crop
Protection
Roundup Ready 2 Corn + Thiamethoxam
seed trt (0.25 mg/seed)
Dow AgroSciences
Cry34AB1 & Cry35Ab1 + Cry1F + RR2 +
thiamethoxam seed trt (0.25 mg/seed)
Cry34AB1 & Cry35Ab1 + Cry1F + Cry38b1
+ Cry1A.105 + Cry 2Ab2 + RR2 + Liberty
Link + thiamethoxam seed trt (0.25 mg/seed)
Cry34AB1 & Cry35Ab1 + Cry1F + Cry38b1
+ Cry1A.105 + Cry 2Ab2 + RR2 + Liberty
Link + thiamethoxam seed trt (0.25 mg/seed)
Roundup Ready Corn 2 + mefenoxam
fungicide + clothianidin seed trt (1.25
mg/seed) + Bacillus firmus I-1582
Roundup Ready 2 Corn + mefenoxam
fungicide + thiamethoxam seed trt (0.25
mg/seed)
Cry34Ab1 & Cry35Ab1 +
Cry1F + Cry1Ab + MIR604 + mefenoxam
fungicide + thiamethoxam seed trt (0.25
mg/seed)
Company
Monsanto
Dow AgroSciences
Dow Background
Dow Background
Pioneer Hi-Bred
International, Inc
Pioneer Hi-Bred
International, Inc
Pioneer Hi-Bred
International, Inc
mefenoxam fungicide + Clothianidin +
thiamethoxam seed trt (0.25 mg/seed)
Pioneer Hi-Bred
International, Inc
Cry34AB1 & Cry35Ab1 +
Cry1F + mefenoxam fungicide +
thiamethoxam seed trt (0.25 mg/seed)
Pioneer Hi-Bred
International, Inc.
mefenoxam fungicide + Clothianidin +
thiamethoxam seed trt (0.25 mg/seed)
Pioneer Hi-Bred
International, Inc
52 Materials Tested for 2011 (Continued)
Common/code name
Pioneer P0461R (RR2)
with Cruiser 250 seed
treatment
Pioneer 35F34PDR
(Herculex XTRA) with
Cruiser 250 seed
treatment
Pioneer 35F37 (RR2)
with Cruiser 250 seed
treatment
Poncho 250
Poncho 500
Poncho 1250
SmartChoice
Syngenta Agrisure 3111
hybrid
(N53C-3111)
Syngenta Agrisure GT
hybrid (N53-W2)
Formulation
Pioneer Background
Pioneer Background
Pioneer Background
Commercially applied
seed trt (0.25 mg/seed)
Commercially applied
seed trt (0.50 mg/seed)
Commercially applied
seed trt (1.25 mg/seed)
Chemical Name
Roundup Ready 2 Corn + mefenoxam
fungicide + thiamethoxam seed trt (0.25
mg/seed)
Cry34AB1 & Cry35Ab1 +
Cry1F + mefenoxam fungicide +
thiamethoxam seed trt (0.25 mg/seed)
Roundup Ready 2 Corn + mefenoxam
fungicide + thiamethoxam seed trt (0.25
mg/seed)
Company
Pioneer Hi-Bred
International, Inc.
Pioneer Hi-Bred
International, Inc.
Pioneer Hi-Bred
International, Inc
clothianidin
Bayer CropScience
clothianidin
Bayer CropScience
clothianidin
Bayer CropScience
AMVAC Chem.
Corp.
5G
Chlorethoxyfos
Transgenic seedcorn
Bt11+MIR604+GA21
Cry1Ab (Bt11) + mCry3A (MIR604) +
mEPSPS (GA21)
GA21
mEPSPS (GA21)
Syngenta Crop
Protection
Syngenta Crop
Protection
53 APPENDIX IV Research Pictures 54