fruit flies Follow us @Realipm for more Bactrocera, Dacus, Ceratitis Real Solutions | Real Farming Founded in 2013 by owners, Louise Labuschagne and Dr Henry Wainwright, Real IPM now employs 250 staff. Our BASIS-qualified field consultants serve both large and small-scale farmers in crops ranging from cereals, field vegetables, fruit and nut plantations, to greenhouse vegetables and ornamental crops. Nairobi has excellent airfreight logistics for exports and our products have Registrations in Kenya, Ethiopia, Tanzania, Ghana, South Africa, Zambia, Zimbabwe, Madagascar, and Canada; and are under-going other pan-African Registrations. Based in Thika, Kenya, Real IPM is a leading producer and toll manufacturer of predatory mites, which are exported globally for other bio-control company brands. Our predatory mites are produced under license from Syngenta Bioline Biopesticides are produced by our laboratories in Kenya. We have global rights to several isolates of Metarhizium under license from icipe international research institute. Together with our own isolates, we have a comprehensive offer for crop protection solutions Real Insights on Fruit fly The Real Insights on Fruit fly was developed by joint MD of Real IPM Kenya, Louise Labuschagne, by bringing together published information from many sources and pooling the extensive field experiences of the Real IPM technical field advisors working in Africa. Louise has over 30 years experience in IPM crop protection and is BASIS-qualified and legally able to give advice on the use of chemical pesticides under UK law. The contents of the Real Insights on Fruit fly are offered as useful information for agronomists seeking to develop more robust IPM programmes in many parts of the world. It is not advice. Growers must satisfy themselves of the local registration issues for both chemicals and biological control agents and the efficacy of these control methods under their local conditions. In order to capitalise on the information provided, growers are encouraged to contact Real IPM Kenya Ltd for both in-depth training and consultancy services to develop appropriate IPM programmes in the country where their crops are grown. www.realipm.com If local Registrations are needed, Real IPM Kenya is willing to discuss potential markets and collaborations that will enable this to happen. Economic Impact of Fruit flies Fruit flies are migratory pests of critical economic importance. Wherever in the world, fruit is grown – fruit flies are causing serious damage. Commercial losses of 100% have been recorded, particularly in mango crops, which are attractive to a number of different fruit fly species.Flies lay eggs in ripening fruit.The larvae, which develop from these eggs, feed on fruit pulp completely destroying the fruit. Fruit flies ‘travel’ the globe in infested fruits as they are traded internationally or smuggled in personal baggage by international travellers. Phytosanitary measures are in place globally to try to reduce this risk. Many important fruit flies are Quarantine Pests. However, when they find a ripe fruit in which to lay eggs, they do not move from this site more than about 40 meters.Life is short – it makes sense to stay in one place, mate, lay eggs and die. This is why there may sometime be regional restrictions in the movement of fruits within a country. Commercial losses of 100% have been recorded, particularly in mango crops, which are attractive to a number of different fruit fly species. Flies lay eggs in ripening fruit. The larvae, which develop from these eggs, feed on fruit pulp – completely destroying the fruit. Bactrocera Invadens (Invader Fly) Bactrocera Cucurbitae (Melon Fruit Fly) 1 Ceratitis Cosyra (Mango Fruit Fly) Know your enemy It is important to be able to identify which species of fruit fly is present in the orchard because • Some of the monitoring and control methods are species-specific – working only for certain types of fruit fly. Incorrect identification of the range of fruit flies present may lead a grower to make assumptions about the level of control expected from purchasing certain monitoring or control products. • Some common fruit fly species are Quarantine Pests in export markets (A1 EPPO List) or have been recommended as Quarantine Pests (A2 EPPO List)1 This means, that if found in exported fruits, there is the risk that future exports might be banned, • Bactrocera invadens, is not only a Quarantine Pest in export markets for mangoes, but also has the ability to reproduce fast and displace local types of fruit flies. This will accelerate the risk of severe damage to the export markets. •Control of all types of fruit fly, irrespective of type, is important because, once Bactrocera invadens is controlled, the other endemic fruit flies will move back into the area and continue to cause damage. Visit www.realipm.com to download a copy of the fruit fly identification poster, developed in collaboration with icipe Growers may order a copy of the Icipe fruit fly identification handbook on the Real IPM website. Payments can be made by M-Pesa or other means on application. [email protected] 1 http://www.eppo.int/QUARANTINE/listA2.htm?utm_source=www.eppo.org&utm_medium=int_redirect 2 The main fruit fly problems The three main groups of fruit flies of concern eratitis, Bactrocera and acus have a very wide crop host range.The following focus list highlights those of economic importance in crops of potential export value. The key in the right column is the reference number for their Quarantine status. This Manual will therefore focus on these types of fruit fly. Bactrocera Bactrocera invadens Invader Fly Bactrocera cucurbitacae Melon Fly Bactrocera zonata Peach Fruit fly Dacus Dacus ciliatus Dacus bivittatus Dacus punctatifrons Dacus vertebratus Dacus lounsbouryi Dacus frontalis Lesser Pumpkin Fly Pumpkin Fly Jointed Pumpkin Fly Ceratitis Ceratitis capitata Mediterranean Fruit ly Ceratatis rosa Natal Fruit Fly Ceratatis cosyra Mango Fruit Fly Ceratitis rubivora Ceratitis fasciventris Farmers need to be vigilant and know what species of fruit fly are present in their farm. This knowledge will influence what type of lure and trap will attract and trap the different species. If farmers know what species of fruit fly are present they can: • Advise when fruit flies are building up and when to start crop protection • Use the correct lure to trap the local type of fruit flies 3 New aggressive species of fruit fly New aggressive species of fruit fly s if the fruit fly problem was not bad enough already – a new species of fruit fly is rapidly spreading throughout the world. ts name is Bactrocera in adens. Bactrocera in adens seems to be replacing indigenous species of fruit fly as it can reproduce so rapidly. ne female Bactrocera can lay up to egg-laying adult fly. fruit fly, laying eggs, which take only here are likely to be more than eggs each generation for generations per year on the uator. ne generations could lea e many hundreds of thousands of offspring in the orchard. 4 days to turn into another Difficult to control with chemicals The fast reproduction rate of fruit flies and their wide crop plant host range contributes to the success of one of the world’s most difficult-to -control pests. The resistance of adult fruit flies to chemical is fast becoming a major issue. Male and female fruit flies mate and then eggs are laid in green or ripe fruit on the tree depending on the species of fruit fly . ggs can e en be laid on ripe fallen fruit. The egg develops into a larvae that feed on the flesh of fruit, making them unmarketable. The fruit may fall to the ground or be thrown away by the farmer on the soil. When in contact with the soil, the larvae exit and pupate in the soil. Finally, an adult fruit fly emerges from the soil and the cycle continues. The Real IPM Fruit Fly programme The naturally occurring, insect-killing fungus, called Metarhizium, is the backbone of the inno ati e eal fruit fly. Bio-intensi e is better than chemical controls for fruit flies. The Real IPM programme attacks the fruit fly life stage in the soil and the egg-laying female flies, as well as the adult flies. Chemical pesticides do not generally target the eggs, larvae or pupae – which leaves these life stages safely developing in fruits or in the soil. Many chemical insecticides are very harmful to parasitic wasps that naturally kill fruit flies. This can make the fruit fly problem worse. 5 Problems with Pesticides Governments have reacted to this problem by financing aerial spraying even over domestic areas where fruit trees in gardens are the source of fruit flies in commercial farms nearby. Resistance to chemical pesticides is compounding the difficulties in controlling fruit flies by solely chemical programmes. idence of resistance to a wide range of pesticides is recorded all over the world. There are health and safety risks attached to this affirmative action. The widespread aerial application of organophosphates for fruit fly in Californian suburbs is no longer permitted. The vast swathes of land infested with fruit flies, encompasses many millions of farmers. ach farmer may ha e a different view of the serious nature of the pest and whether he or she can afford to apply a chemical control for fruit flies. Less harmful active ingredients such as spinosad are now used in regional aerial spraying programmes. Pockets of fruit flies are likely to rein ade treated areas ery uickly from untreated farms. 6 Programme The IPM crop protection programme has five phases: 1. Monitoring of the increase in fruit flies at the beginning of fruit fly season . imely application of soil drench of etarhi ium under the trees to kill the pupae of all types of fruit fly. his will also kill mango wee il and thrips. epeat this e ery weeks if fruit fly le els high or a uarantine pest. . imely set up of eal etarhi ium uto- issemination e ices per hectare for protein bait de ices be hung in the trees to infect li ing fruit flies and thereby contaminate and kill the rest of the population. If methyl eugenol is used instead of protein baits in the de ices – then only d de ices are needed per hectare – but it will only attract male Bactrocera not other species . . . . . e-charge the et spores in the de ices e ery weeks during the fruit fly season. f necessary, foliar sprays of etarhi ium in the canopy at the correct time of day to contact fruit flies that are actively feeding in the canopy. ollect fallen fruit and place in a parasitoid har esting tent see instructions later in manual se COMPATIBLE SPRAY PROGRAMME for all other pests and diseases that will NOT KILL fruit fly parasitoids. Seek ad ice from eal . oid use of pyrethroids and organo-phosphates. IMPORTANT uto-dissemination de ices are a lure where li e fruit flies come and go, after being infected with an insect-killing fungus, called Metarhizium. n de ice is not a mass-trapping de ice. he fruit flies must remain ali e for the infection of the local population with the Metarhizium. f sufficient de ices are used per hectare, then up to could be infected with the etarhi ium within weeks. of the local population of fruit flies s soon as the de ices are in the crop, any mass trapping de ices must be remo ed because they will kill the fruit flies that are infected by the etarhi ium from the uto- issemination e ices de ices . f this happens – there will be no hori ontal transmission of the Metarhizium and less fruit flies will be killed. t takes to days for a fruit fly to die from the infection of have infected more fruit flies. 7 etarhi ium. n this time they will REAL METARHIZIUM REAL METARHIZIUM Real IPM (Kenya) Ltd has commercialised a number of entomopathogenic fungi (Metarhizium anisopliae) from icipe an international research institute based in Nairobi, Kenya. Metarhizium anisopliae is a ubiquitous, naturally in the soil and when taken out of the soil and mass- produced as a bio-insecticide, it will not persist for more than about 12 hours in the canopy because UV light kills it. Metarhizium 69 has been tested Metarhizium 62 has proved effective against aphids and leafminer Metarhizium 78 infects spider mite, tarsonemid mites, varroa mites and leafminer. PEST-SPECIFIC METARHIZIUM ISOLATES If the Metarhizium spore lands on an insect pest’s body and the spore can ‘recognise’ the pest, only then will it germinate. The process of ‘recognition’ makes the many different isolates of Metarhizium are not ‘broad spectrum” in the same way as some chemical insecticides. This is why each different Metarhizium isolated from the soil is named with a different number. 8 Advantages of Metarhizium . . . . It is compatible with predatory mites and parasitoids. There are no pesticide residue issues, or re-entry intervals with this product. It is safe to humans and the environment. There is no recorded resistance of pests to Metarhizium, due to its many modes of action . Metarhizium spores are sprayed onto the soil and in the canopy. The fungus can grow saprophytically on organic matter in the soil, where it can reduce the number of fruit fly, thrips and weevils emerging as adults from the pupae in the soil. he addition of a layer of organic matter sawdust, rice husks, coffee husks etc. on the soil surface will promote this effect. Metarhizium life cycle Metarhizium spore germinates on contact with insect’s body then forms an apressorium that helps it penetrate the cuticle. The fungus grows through the insect’s cuticle, into the haemolymph, where it proliferates and kills the target pest. 9 10 © Real IPM (Kenya) Ltd www.realipm.com Fruit flies infected with Metarhizium, spread the fungus by social contact with males and females. hey die in days, so there are fewer eggs laid in fruit If fruit fly levels are very high, a foliar spray of Real Metarhizium can be made in the canopy, when fruit flies are present Male and female fruit flies of all species are attracted to the protein bait in the eal utoDissemination Device Metarhizium does not kill fruit fly parasitoids so more of them will survive foliar sprays and kill more fruit flies Fewer adult fruit flies emerge from the soil, so there is less egg-laying in the fruit Real Metarhizium is drenched onto the soil to kill fruit fly pupae 10 More fruit fly parasitoids will emerge from the soil because Metarhizium will not kill them. Therefore parasitoids can make a greater contribution to fruit fly control in a Real IPM programme Real IPM Auto-Dissemination Device he eal de ice is a -piece unit which attracts fruit flies into a top section, where it becomes contaminated with an insect-killing fungus called Metarhizium. The fruit flies cannot become trapped and killed in the li uid lure in the base section, because of the di iding section between the lure and the Metarhizium arena. 11 Key uncontaminated flies contaminated flies eal et Spores Field flies continue to spread the eal et spores to other fruit flies contamination of fruit flies with Real et in wks Contaminated flies from de ice infect other flies in the field when mating or grooming Real AD device food bait lure will attract male and female adults Bactrocera, acus B they cannot drown in the li uid food bait because there is a dividing screen that prevents them entering 12 eratitits . Mass Trapping Lure No auto dissemination if mass trapping lure is used within 12m of an AD device There is no dividing screen in a mass trapping lure so flies that enter drown in the lure Fruit flies contaminated with eal et from the de ice could be killed in a mass trapping lure before they can cross -contaminate the rest of the fruit fly population 13 Fruit fly life cycle Real IPM Met 69 soil drench Fruit flies attack fruit Fruit fly parasitoids not killed by fruit flies emerge from soil and attack fruit No fruit flies emerge from soil x Fruit fly pupae et Fruit fly larvae from fallen fruit will pupate in the soil x x et kills fruit fly pupae in the soil x Fruit fly pupae eal et soil drenches will not kill parasitic wasps that kill either fruitfly pupae. he parasitoids can kill MANY fruit fly lar ae pupae. hemical pesticides will kill fruit fly parasitoids. CONSERVE fruit fly parasitoids - use eal et 14 Real IPM Auto-dissemination Device Auto-dissemination is a technology, which has been tested by ICIPE Scientists and found to be a very effective means of controlling fruit flies. It uses the live fruit flies themselves to transmit an insect-killing fungus, called Real Metarhizium 69 throughout the population. Real IPM AD device The modified Lynefield Lure is a bucket with a lid. It has holes in the side so that flies can enter, after being attracted by the protein bait inside the bucket. The protein bait will be separated from the rest of the bucket, so that flies cannot fall into the bait and get ‘trapped’. If they are trapped and killed – they cannot ‘auto-disseminate’. The Inside surface of the bucket is lined with velvet material that will be sprayed every 6 weeks with Metarhizium 69. This is called ‘re-charging’ the trap. The flies move over the surface of the velvet and pick up Metarhizium spores. They will not die for another 2 to 5 days, depending on how many spores they picked up and what the temperature is. High temperatures, hasten death. 15 Field Guidance on AD device use. • here will be – de ices per hectare using the protein bait that attracts males and females of all the types of fruit flies. If methyl eugenol is used instead of the protein bait, only de ices are needed per hectare but it will only attract male Bactrocera . • he de ices will be hung from trees, spread e enly throughout the orchard. devices should be hung so that they are about 1.5 meters from the ground. • ag the trees with a bright yellow tape so that they can easily be found again, for weekly inspections and when it is time to re-charge them with more Metarhizium. • ecords should be kept of the recharge dates and repairs that need to be made to the devices. he ICIPE Scientific Results after correct AD device use • ess than of fruits infested with fruit fly from orchards treated with hereas there was infested in orchards without de ices. de ices alone. • hen combined with the soil treatments with control achieved. • uto-dissemination may ha e a greater impact than mass trapping on fruit fly numbers as long as there are no other lures in the field and the de ices are combined with SOIL APPLICATIONS of Metarhizium 69 for fruit fly pupae. 16 etarhi ium, there would be e en greater Auto-dissemination devices need to be re-charged every six weeks, according to trials results from icipe scientists. ICIPE scientists found that the etarhi ium uto- issemination de ices provided as good a control as the malathion. Malathion is a broadspectrum organo-phosphate insecticide, which was used for many years for the control of fruit flies. The Metarhizium spores remain active for up to six weeks, before needing to be re-charged Most farms are now moving away from using malathion because of its perceived harmful effect on the environment. The Metarhizium de ices gi e as good control as malathion, if used correctly in an IPM programme This result was achieved without adding the Metarhizium application to the soil, which would have even further reduced the fruit fly numbers. 17 Soil applications of Real Metarhizium 69 All fruit fly species have part of their life cycle in the soil. ICIPE scientists have tested the efficacy of Metarhizium applications to the soil and the persistence of the control of fruit flies achieved. The Real IPM programme will make a minimum of two applications of Metarhizium 69 to the soil. Soil drenches of Real Metarhizium provide significant control of all species of fruit flies. The above graph shows the percentage difference in the number of adults trapped in an orchard before and after treatment of the S with etarhi ium oil a ueous formulation compared to the broad-spectrum organophosphate, diazinon. This result was recorded only 2 weeks after the soil application. At least two soil drenches of Real Metarhizium are advisable during the fruit fly season. The above graph shows the difference in fruit fly numbers 185 days after the single SOIL application of Metarhizium. Note the INCREASE in fruit fly numbers in the treated soil. 18 What is the effect on fruit fly parasitoids of the SOIL treatments? Fruit fly parasitoids will lay eggs inside the fruit fly pupae that are in the SOIL. The data presented from the ICIPE work above shows clearly that if chemical pesticides are applied to the soil there is an IMMEDIATE and TOTAL ANNIHILATION of fruit fly parasitoids straight after the application. Fruit fly parasitoids are not affected by drenches of Real Metarhizium in the soil. 19 Behaviour of Fruit fly adults Knowing where and when fruit fly adults are likely to be in a crop, will allow growers to target their canopy sprays of Met 69 more effectively. The best time of day to spray for adult fruit flies in the crop is 9-10 Am and 4-5 Pm crop in the middle of the day. They are more likely to be found in the windbreaks 20 Fruit Fly Parasitoids Real IPM will begin the mass production of fruit fly parasitoids for distribution to various African countries, following Registration. Growers will be kept informed of their availability by following us on Twitter. Conservation of natural enemies of fruit flies Although the chemicals now used for aerial spray programmes are considered safe for humans, they are still likely to have some negative effect on the tiny naturally occurring parasitic wasps, which lay their eggs in the larvae and pupae of fruit flies. In the meantime, the Real IPM programme using Metarhizium 69 will not kill the indigenous, naturally occurring parasitoids. The Real IPM programme CONSERVES fruit fly parasitoids. Growers may believe that cheap broadspectrum pyrethroids can save them money on their spray programme; but they can also ‘cost’ growers dearly because they kill fruit fly parasitoids. According to icipe, even spinosad is known to have some negative effect on fruit fly parasitoids. After a single spray of a pyrethroid, up to of them can be killed and this harmful effect on fruit fly parasitoids could last up to weeks. In spite of its very small size, each female parasitic wasp can kill up to fruit fly larvae or pupae by laying an egg inside the fruit fly. Parasitoids are able to fly and seek out fruit fly larvae and pupae that have not been infected with Metarhizium yet. 21 Conserve, collect and recycle parasitoids Fruit fly parasitoids may not need to be bought from commercial companies to be applied to the crop, if a compatible spray programme is implemented. They will build up in high numbers if there are fruit fly larvae and pupae in the crop. It is likely that most of the fruit fly larvae and pupae in the crop will be in fallen fruit on the orchard floor. If these fruit are collected and placed inside a fruit fly parasitoid collection tent – the parasitoids can be allowed to emerge and fly back into the crop or collected and taken to fruit fly hotspots. The tent is a simple device made from a tent over the top of a box of fallen fruit. The netting mesh size needs to be small enough to allow the exit of the fruit fly parasitoids but not the fruit flies, which are bigger than parasitoids. Make sure that the edges of the net are securely fixed to the box containing the fruit – otherwise fruit flies may escape as well. Collecting fallen fruit with fruit flies is good orchard hygiene, which will also remove black rot (Aspergillus), bacterial canker (Xanthomonas) and anthracnose (Colletotrichum). 22 Community –wide programmes There is therefore a constant threat of invasion Studies have revealed that fruit flies can fly into fly-free areas. Early detection is critical in for up to 100 hours, non-stop, easily moving preventing populations from establishing. more than 4 kilometres in search of fruits to Africa is home to several endemic populations 2 lay their eggs. It is known that some fruit of fruit fly that are Notifiable in potential export flies will move more than 20 kilometres in a markets. This has already inhibited free trade, lifetime. due to interceptions of exotic fruit flies being Once a fruit fly species becomes endemic it exported into these zones. is more difficult to eradicate. Prevention is particularly important for Bactrocera invadens (African Invader Fly) because it reproduces very fast and can replace other species in the locality. In Kenya, Bactrocera invadens is considered the dominant fruit fly species in Kenya3 . The lack of specificity in crops attacked, undermines the effectiveness of crop specific protection strategies. It is essential that communities and regions identify potential host crops and design an IPM programme for all potential host crops, irrespective of the economic impact on an individual crop. This would reduce the commercial risk of fruit flies building up in one crop, where it is not considered worthy of control, and migrating into high valuecrops where the economic impact is severe. 2 Fletcher (1989) 3 Pers. Comm. Dr. Sunday Ekesi ICIPE Nairobi. 23 Fruit fly populations may be higher, where small-scale farmers have a limited number of mango trees, interspersed between other hosts such as coffee, passion fruit, and wild guava trees. Non-treated crops act as a ‘bridge’ between the fruiting seasons of the mango trees Real IPM in-company field efficacy trials The field trials were carried out in commercial mango orchards in Meru, Embu, Makueni and Machakos. All mango farmers involved have provided testimonials on the extraordinary efficacy of the Real IPM fruit fly programme. www.realipm.com Harvested fruit was incubated and dissected to recover fruit fly larvae. The Real IPM fruit fly programme delivered 80 to 92% control in Embu and 100% control in Meru 24 “ I have lost 100% of my mangoes to fruit fly in some years. I am back in business thanks to the Real IPM programme. With a combination of the soil drenches and the AD devices – I achieved 90% control. The middleman who sell my mangoes to Nairobi shops was so happy that he told his customers he would give them 100 Ksh for each mango they found with a fruit fly in it!” Henry Ngari Mango Farmer Embu 25 “I had my best harvest in 2013, but only sold 50% due to fruit fly damage. But when Real IPM came in 2014 they taught me how to manage the fruit fly menace and this season i have sold 90% of my produce.The fruit that I could not sell was not damaged by fruit fly but due to physical damage when it fell from the tree” Daniel Kirai Mango Farmer Meru 26 French scientists in Reunion Islands discovered that planting a barrier of maize plants around low growing crops, concentrated fruit flies in a predictable manner at certain times of day. Fruit flies roosted in the maize crop where they went for shade. This made them an easier target for spraying a control agent. It is likely that this tactic may also have a part to play in the control of leafminer 27 Do not use mass trapping in the maize border because it will lure and kill fruit flies from 12 m away (within the crop). This will reducethe efficacy of the nearest Met 69 AD inside the crop Other Fruit Fly controls Sterile Insect Technique (SIT) Many millions of $US dollars have been spent in various countries around the world on the mass production and release of sterile male fruit flies of very specific economic species. These SIT factories are sophisticated laboratories capable of rearing and sterilising insects with gamma-irradiation. Many millions of SIT males can be produced every week. The basis of this technology is the understanding that each female fruit fly will mate only once and that if the sperm is not viable, then no eggs will result. The objective of these releases is for the sterile male fruit flies to compete with the ‘wild type’ (normal) fruit flies in the orchard. The sterile males must ‘get to the female fruit flies’ before the potent local fruit fly males. For the SIT to effectively control the local fruit fly population, there are two important factors: • The numbers of SIT released must overwhelm the local male fruit flies, other wise it is an expensive waste of time. • The vigour of the gamma-irradiated sterile males must be sufficient to keep them alive, after release, for as long as a healthy male. Otherwise large numbers of male SIT must be release every week – sometimes more than once per week. This is expensive. For the above reasons, SIT is not effective against dense infestations of fruit flies. It is more likely to have an effect on fruit fly populations when combined with other fruit fly crop protection measure, which also conserve fruit fly parasitoids. Will the Real IPM Auto dissemination and soil applications of Met 69 reduce the need to invest in expensive SIT technology? 28 Realipm @Realipm Real Solutions | Real Farming www.realipm.com Insights Real IPM (Kenya) Ltd P.O Box 4001- 01002 Madaraka, Thika, Kenya (+254) 0725 806086 | [email protected] | Director Dr Henry Wainwright [email protected] Director Louise Labuschagne [email protected] www.realipm.co.uk Real IPM South Africa Pty Ltd Managing Director Jean Kuiper [email protected] Elephant Vert Technical Manager Benoit Gossaert [email protected] South Africa, Zambia, Zimbabwe Morocco, Mali, Côte d'Ivoire, Ghana, Madagascar, Senegal Burkina Faso Crop Defenders Ltd Managing Director Dr. Ishtiaq Rao [email protected] Canada Real IPM Tanzania [email protected] Tanzania Realipm The information contained in Real Insights does not constitute advice. Farmers should satisfy themselves of the registration and efficacy issues involved in implementation
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