HS/ZS 2e
THE CYTO SYSTEM
For perfect preparation
Quality Products. Lifetime Care.
HERAEUS®® CYTO SYSTEM
FOR THE PREPARATION OF MICROSCOPE SLIDES
For the sedimentation of cells and
similar biological material directly
from the sample to the microscope
slide, Heraeus offers the specially
developed cyto system. Its design is
such that it can be used with all
Heraeus general purpose centrifuges.
The principle
Open cyto containers are placed on to
the slide and are loaded with the sample.
Due to the centrifugal force the cells
contained in the suspension settle on the
slide and stick to it. The containers are
taken off after centrifugation and the
slides are gathered. The cell sediment on
the slides is prepared for microscopy
through fixing and dying, as usual.
■ Ideal
for the production of slide preparations with any cells
■ Suitable for every Heraeus general
purpose centrifuge
■ Filter or decanter method
■ Sample volumes of 50 µl to 3 ml are
possible
■ Double samples on every slide
The accessories
The rectangular cyto containers are made
of plastic and have two holes, each fully
drilled, which are sealed by o-rings on
the slide. The tops are sealed with a lid.
The two chambers ensure that the
sample itself and a reference sample can
be prepared on the slide at the same
time. The containers are available with
cylindrical (1.5 ml) and conical (3 ml)
holes. The conical container with its
smaller sedimentation area and larger
volume, allows cells to be concentrated.
The holder, the slides and the container
clip together for loading into the centrifuge.
The cyto containers are transparent,
resistant to chemicals and are autoclavable.
The cyto holder is made of a flat metal
plate with DIN-slide dimensions (25 x
76 mm). Two side spring metal sheets
hold the container on to the slide.
Cyto accessories are used for
the centrifugation of cells on
microscopic slides.
The process
To achieve high quality results the sample's cell concentration and the sample
volume to be used, must be determined.
A cell density of 2,000 to 5,000 per mm2
at a diameter of 5 to 10 µm serves as a
standard value. The choice of cyto container with their different sedimentation
areas is determined by the cell concentration, and volume of the sample.
In principle, two methods are possible –
the filter method and the decanter
method.
The filter method
A slide is put into the vessel holder and a
suitable filter card is positioned between
the container and the slide. Micro tubes
with a hole in the bottom, which are
placed into the container holes, are used
to hold cell suspensions. Due to capillary
forces, the sample only enters the
container once the centrifugal force is
applied. The suspended cells reach the
glass surface and stick to it.
The micro tubes prevent sample loss by
stopping a flow on to the filter paper prior
to centrifugation. Depending on the
suspension's cell concentration, the
sample quantity needs to vary. For this
method the recommended sample
volumes are between 50 to 200 µm. The
smaller the sample quantity the better it
is absorbed by the filter paper. With
larger volumes liquids collect in the
bucket's cavity underneath the slide.
above which is a little residual liquid,
which must be dried prior to dying or
fixing.
With the filter method the cell sediment is
considerably drier than with the decanter
method.
The decanter method
The principle behind this method is that
the cyto container and the slide form a
tight vessel. The rubber pad which
comes with the vessel holder is placed
underneath the slide. With the assistance
of a pipette the sample material is filled
directly into the containers with the
o-rings, facing the glass.
Following centrifugation the cyto holder
with the container is taken out and the
liquid is decanted or sucked away. The
cell precipitate forms a round spot,
The centrifugation
For centrifugation the loaded vessel
holders are either placed into the Labofuge 400's special cyto bucket, for which
an aerosol-tight lid is available, or into the
support inserts. Using the support inserts
they can be used in any rectangular
swing-out bucket. For gentle cell treatment centrifugation needs to take place
with the brake switched off. The recommended centrifugation period is five to
ten minutes.
Depending on the particular sample
material, it is essential that all parameters
are optimised.
WHICH ACCESSORIES FOR WHICH METHOD?
Accessory1)
Filter method
Decanter method
Micro tubes
0.75 ml or 1.5 ml
–
Cyto container
1.5 ml cylindrical or
1.5 ml cylindrical or
3 ml conical
3 ml conical, incl. lid
Filter cards
for cylindrical
or conical containers
–
Slide
yes
yes
Vessel holder
yes
yes with rubber insert
Support insert
Cyto bucket
for Megafuge, Varifuge
for Megafuge, Varifuge
and Cryofuge
and Cryofuge
for Labofuge 400
for Labofuge 400
Lid
1) For
order numbers see overleaf.
CYTO ACCESSORIES – AREAS OF APPLICATION
Centrifuge
Rotor order no.
Bucket order no.
Number of containers Recommended speed (rpm) for
filter method
decanter method
Labofuge 400/400e/400R
75008179
75008176
4
Megafuge
75002704
500 – 1400
700 – 2000
75002252
4
400 – 1400
500 – 2000
75008030
4
400 – 1400
500 – 2000
75003360
–
4
500 – 1400
600 – 2000
Megafuge 2.0/2.0 R/2.0 RS
75003360
–
4
500 – 1400
600 – 2000
Varifuge 3.0/3.0 R/3.0 RS
75008074
76008078
8
400 – 1400
500 – 2000
75005315
–
8
400 – 1400
500 – 2000
75006606
76008078
12
400 – 1400
500 – 1900
1.0/1.0 S/1.0 R/1.0 RS
Cryofuge 6000 i /8500 i
ORDER NUMBERS
Accessory illustration
Description
Order no.
0.75 ml micro tubes for 75003415,
75001153
100 each with hole in bottom for filter method applications
1.5 ml micro tubes for 75003416,
75001152
100 each with hole in bottom for filter method applications
1.5 ml cyto container
75003415
cylindrical, incl. lid, 8 mm Ø, 50 mm2 sedimentation area, 35 mm h
3 ml cyto container
75003416
conical, incl. lid, 17 mm upper Ø, 4,5 mm lower Ø, 16 mm2 sedimentation area, 35 mm h
Replacement lid
76003422
for both cyto containers
Replacement o-rings
75003407
for 75003415, 10 each
Replacement o-rings
75003408
for 75003416, 10 each
Filter cards for container 75003415
76003424
100 each for filter method applications
Filter cards for container 75003416
76003425
100 each for filter method applications
Vessel holder
75003414
to hold a microscope slide and
a cyto container
Support insert
75003417
to hold a vessel holder for use in rectangular buckets
(all general purpose centrifuges except Labofuge 400)
Cyto bucket
75008176
for Labofuge 400 with swing-out rotor 75008179
Lid for 75008176,
75003426
tight closing, made of plastic
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