Anurans Frog and toad External fertilization Urodeles Salamander Internal fertilization Environmental hazard affecting amphibian development • • • • • • • Loss of wetland Increased ultraviolet radiation Pollution of breeding sites Low PH Toxicity of aluminum Dry weather Sudden temperature changes Stages in amphibian development 1. Unfertilized 2. Gray crescent 3. 2-cell 4. 4-cell 5. 8-cell 6. 16-cell 7. 32-cell 8. Mid cleavage 9. Late cleavage 10. Early gastrula , dorsal lip forming 11. Mid gastrula 12. Late gastrula 13. Neural plate 14. Neural fold 15. Rotation 16. Neural tube 17. Tailbud 18. Muscular response 19. Heartbeat 20. Gill circulation, hatching 1. Unfertilized • Mesolecithal type • Telolecithal type 2. Gray crescent 3. 2 – blastomers stage 4. 4 – blastomers stage 5. 8 – blastomers stage 6. 16 – blastomers stage 7. 32 – blastomers stage 8. Mid cleavage (Morula stage) 9. Late cleavage (blastula stage) 10. Early gastrula , dorsal lip forming 11. Mid gastrula 12. late gastrula 13. Neural plate 14. Neural fold 15. Rotation 16. Neural tube 17. Tailbud 18. Muscular response 19. Heartbeat 20. Gill circulation, hatching Organization on tadpole Examine cross section of frog embryo at hatching stage through head and developing eye Neural tube notochord somites Heart foregut Adhesive glands Examine cross section of frog embryo at hatching stage through region of heart and gills 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Examine cross section of fertilized egg of frog Examine cross section of 2 – blastomers stage of frog Examine cross section of 4 – blastomers stage of frog Examine cross section of 8 – blastomers stage of frog Examine cross section of 16 – blastomers stage of frog Examine cross section of blastula stage of frog Examine cross section of Mid gastrula of frog Examine cross section of late gastrula of frog Examine cross section of frog embryo shows neural plate Examine cross section of frog embryo shows neural fold Examine cross section of frog embryo shows neural tube Examine cross section of frog embryo at hatching stage through head and developing eye 13. Examine cross section of frog embryo at hatching stage through region of heart and gills T
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