end
Secondary−mean−coverage cutoff
50
Primary−read−start threshold
50
50
40
40
40
30
30
30
20
20
20
10
10
10
0
TU8003
TU8003
TU8004
TU8007
TU8006
TU8004
TU8009
TU8007
TU8006
TU8009
slr8014
ssr8013
slr8014
ssr8013
start
●
00.0 0.0
0
2000
sll8001
sll8001
●
4000
sll8002
sll8004
sll8006
8000
ssl8008
sll8007
ssl8008
sll8009
sll8007
ssl8010
ssl8010
sll8009
TU8005
TU8005
TU8002
sll8012
sll8011 sll8012
sll8011
TU8008
TU8008
start
0
●
start
−40
40
60
60
80
1
40
−60
20
−80
20
●
10000
0
10
20
30
40
50
0
0
−20
0 −50
6000
sll8006
ssl8005
TU8002
●
●
0
sll8002 ssl8003 sll8004 ssl8005
ssl8003
0
0
end
80
Untreated
15°C
42°C
− CO2
Dark
−Fe
HL
−N
−P
Exp. P.
Stat. P.
Sqrt of read number
TU8001
TU8001
●
0.0 0.0
0.0 0.0
●
●
0
10
20
30
40
50
0
40
30
20
10
0
start
−20
Sqrt
0 of
0 read
endnumber
0
0
1
●
20
40
30
20
10
0
0
1
40 0
0
0.0 0.0
1
start
250
Secondary−mean−coverage cutoff
250
200
end
Primary−read−start threshold
250
start
150
150
100
50
1
150
100
200
50
200
100
50
●
0
TU8009
TU8013
TU8009
slr8014
slr8014
slr8015
slr8015
TU8015
TU8013
TU8017
TU8015
TU8017
TU8018
TU8020
TU8018
TU8022
TU8020
TU8022
slr8016
slr8016
slr8021
slr8021
start
−10 0.0 0.0
10000
14000
sll8017
TU8011
TU8010
TU8011
TU8010
sll8018
16000
0
sll8018
sll8017
TU8012
TU8014
TU8012
TU8014
●
18000
sll8020
sll8019
sll8020
sll8019
TU8019
TU8016
TU8021
TU8019
TU8016
TU8021
start
0
10
20
30
40
50
60
70
0
0
●
−20
start
−40
40
60
60
80
1
40
−60
20
−80
20
●
20000
0
−40
0
10
20
30
40
50
60
70
0
12000
slr8023
0
0
slr8022
slr8022 slr8023
−100
end
80
100
Untreated
15°C
42°C
− CO2
Dark
−Fe
HL
−N
−P
Exp. P.
Stat. P.
100
Sqrt of read number
●
0.0 0.0
0.0 0.0
start
●
0−70
1
Sqrt
0 of
0 read
endnumber
0
0
0 100
1
250
300
250
200
150
100
50
0
0.0 0.0
0
0
300
250
200
150
100
50
0
60
Secondary−mean−coverage cutoff
60
50
50
40
40
40
30
30
30
20
start
20
20
10
10
10
0
40
30
20
10
0
start
●
TU8025
0.0 0.0
TU8025
slr8026
slr8023
slr8026
slr8023
TU8026
TU8029
TU8028
slr8030
slr8029
slr8029
TU8029
TU8028
TU8026
0.0 0.0
TU8023
TU8023
slr8030
start
00.0 0.0
20000
0
ssl8024
ssl8024
22000
24000
ssl8028
sll8025
26000
0
28000
sll8027ssl8028
sll8025
sll8031
sll8027
sll8031
sll8033
TU8027
TU8024
●
sll8032
sll8032 sll8033
TU8027
TU8024
●
0
0
●
start
sll8034
sll8034
30000
0
5
0
5
−10
Sqrt
0 of
0 read
endnumber
0
0
1
●
20
40
30
20
10
0
0
1
40 0
0
0
10
10
0 −20
15
15
20
0
●
start
−10
20
0
−20
20
30
30
40
1
20
−30
10
−40
10
end
40
Untreated
15°C
42°C
− CO2
Dark
−Fe
HL
−N
−P
Exp. P.
Stat. P.
Sqrt of read number
1
50
0.0 0.0
end
Primary−read−start threshold
60
end
Secondary−mean−coverage cutoff
80
Primary−read−start threshold
80
80
60
60
60
40
40
40
20
20
20
0
TU8031
0
0.0 0.0
TU8030
TU8031
TU8030
slr8036
slr8036
TU8032
TU8032
TU8034
TU8038
TU8039
TU8036
TU8038
slr8038
slr8037
slr8044
slr8038
slr8037
slr8044
start
0
●
30000
32000
00.0 0.0
sll8034
0
10
20
30
40
50
60
0
34000
ssl8039
sll8035
ssl8039
TU8033
TU8035
TU8033
TU8037
TU8035 TU8037
sll8040
38000
sll8040
TU8040
sll8042
ssl8041
sll8043
TU8041
TU8042
TU8042
0
0
10
20
30
40
50
60
0
start
10
0
●
20
−20
20
30
−30
30
40
−40
40
50
−50
50
−60
60
60
end
●
40000
ssl8041sll8042 sll8043
TU8041
TU8040
start
●
−10
0 −60
36000
0
sll8034sll8035
10
1
TU8039
TU8036
TU8034
−30
Sqrt
0 of
0 read
endnumber
0
0
1
●
0
1
20
start
Untreated
15°C
42°C
− CO2
Dark
−Fe
HL
−N
−P
Exp. P.
Stat. P.
Sqrt of read number
50
40
30
20
10
0
40
50
40
30
20
10
0
●
0.0 0.0
0
0
0.0 0.0
1
start
end
Secondary−mean−coverage cutoff
60
Primary−read−start threshold
60
60
40
40
30
30
30
20
20
20
10
10
●
0
60
50
40
30
20
10
0
start
●
TU8045
TU8045
TU8044
slr8044
slr8044
slr8045
slr8045
slr8046
TU8046
ssr8047
slr8046
ssr8047
start
00.0 0.0
40000
41000
42000
0
43000
sll8048
sll8049
sll8048
0
10
20
30
40
50
●
0
●
sll8049
TU8043
TU8042
TU8043
TU8042
start
−20
44000
0
10
20
30
40
50
0
0
0
20
40
−40
40
−60
−20
start
20
60
60
80
80
end
●
0
0
−80
1
TU8046
0.0 0.0
0.0 0.0
TU8044
●
0 −50
1
Sqrt
0 of
0 read
endnumber
0
0
0
20
1
40
60 0
0
60
50
40
30
20
10
0
0.0 0.0
1
40
10
50
Untreated
15°C
42°C
− CO2
Dark
−Fe
HL
−N
−P
Exp. P.
Stat. P.
Sqrt of read number
50
start
50