Producing Hybrid Walleye in a Commercialscale Water Recirculating System Gregory Fischer, Facility Manager Kendall Holmes, Advanced Aquaculture Technician UW-Stevens Point Northern Aquaculture Demonstration Facility Why Hybrid Walleye(Saugeye)??? Hybrid walleye have many characteristics that lend themselves to aquaculture production intensively. Ability to raise and spawn broodstock intensively and out of season. Ability to do entire rearing cycle intensively from egg-fry-fingerlingmarket size fish on commercially available feeds. (no plankton or brine shrimp needed!!!). Growth rates up to 2.0grams per day (3-4x better than yellow perch!!). Good feed conversions (1.2-1.4) during growout. Existing markets in the Midwest and good fillet market prices ($1012 lb). Potentially several market sizes from petite fingerlings in <6months to larger sized fish in 1 to 3 years. Good rearing temperatures and water quality for possible aquaponics Objectives The UWSP-Northern Aquaculture Demonstration Facility (Bayfield, WI) conducted a three year pilot project to evaluate and demonstrate commercially available new technologies for producing purebred and hybrid walleye for stocking and food fish. The project consisted of different areas including: 1) Intensive production and seasonal advancement of walleye and sauger broodstock. 2) Production of large walleye (>300mm)(12inch) on commercial feed for NR stocking. 3) Production of hybrid walleye to food market size(454g)(1.0 pd) using intensive rearing and recirculation systems. Materials and Methods Experimental Tank Setup for Phase I-II Intensive Rearing •230 L (60 gal) round tanks •Sidewalls painted black •Gray bottom •Adjustable lighting •Directional flow-thru 20ºC(70ºF) water (2-6 lpm) •Clay(old mine #4) •24 hr feeders •Surface spray •Removable screens •Daily cleaning system Water inflow pipe Surface spray Directional water flow Center screen Feeder Otohime fry feed 200 -1400 m Nelson Silvercup walleye grower 1.0mm Materials and Methods Recycle System Used For Phase III-IV Intensive Growout RAS Parameters: •53,000 L water capacity •33 m³ tank culture space •Fluidized sand biofilter •Drum Filter •Dual drain circular tanks •Oxygen cone •In sump electric heater •23ºC (74ºF)Water temp. Results Overall Survival Percentages Intensive Rearing 100 90 0-38 mm Phase III 80 38-51 mm % survival 70 Phase II 51-102 mm 60 50 Phase I 102-152 mm 40 30 48% 48% >90% 20 10 0 Phase and fish length 152-239 mm Results Phase II-Fry Culture Average Survival Phase I Fry at Different Densiities 45.0% 39% 40.0% 35.0% % survival 30.0% 25.0% 20.0% low density high density 16.7% 15.0% 10.0% 5.0% 17fry/L 46fry/L 0.0% Rearing Density Results Phase 1- Fry Culture Observed Phase I Fry Mortality 45 41% 40 Overall mortality % 35 30 25 18% 20 15 10 5 0 Low Density High Density Walleye/Hybrid Walleye: Cannibalism •Starts immediately •Unobserved mortality •Can account for low survival rate in ponds and in indoor tanks Results Phase 1- Fry Culture Unobserved Phase I Fry Mortality ie. Cannibilism 60 51% Overall mortality % 50 40 33% 30 20 10 Low Density 0 High Density Results Phase II Fingerling Rearing Survival % 100.0% 90.0% 93.5% 80.0% % Survival 70.0% 60.0% low density (9 days) 50.0% 38.9% 40.0% 30.0% 20.0% 10.0% 2.3 f/L 9.9 f/L 0.0% Rearing De nsity and Days in Tank high density (13 days) Results Growth Rates H y b rid Wa lley e v s P u reb re d Walle y e G ro w th in Weig h t 450 Phase II Phase I Early Lifestage Rearing 400 350 Phase III Growout in Recycle System G ra m s 300 250 200 S E -10 S E -09 150 WE -10 WE -09 100 50 0 366 346 317 288 262 251 228 222 Nov 201 D a y s P o s t Ha tc h 197 Aug 187 173 170 159 156 142 129 127 116 105 98 91 85 73 June 67 64 58 52 37 29 20 April March 1 year old Hybrid Walleye (1.0kg) Results Hybrid Walleye Feed Rates and Conversion Ratio 20 20 18 15 16 14 12 10 7.5 8 7 6 4 4 2 2 0 2 TBW FD(%) 1.6 1 2 Grow th Phase FC 3 4 Recycle System Water Quality Operating Parameters Parameter Average Value Temperature (ºC) 23 Dissolved oxygen (mg/L) >7.0 pH 7.7 Carbon dioxide (mg/L) 6.8 Total ammonia nitrogen (mg/L) 0.011 Nitrite nitrogen (mg/L) 0.061 Calculated unionized nitrogen (mg/L) 0.0003 Total suspended solids (mg/L) 2 Alkalinity (mg/L) 151 Commercial Intensive Production Recommendations • Phase I High density/shorter time frame in tank Water Temperature: 20ºC Density: 30 days @ 46 fry/L Feed: Otohime TBWFD: >20% @ 24hr *Grade in tank every week as soon as possible from Phase I-III. • Phase II Water Temperature: 20ºC Density: 10 days @ 4 fish/L Feed: Nelson SC Walleye Grower TBWFD: >15% @ 24hr Production Recommendations • Phase IIb-Intensive Culture Water Temperature: 20ºC Density: 4 fish/L Feed: Nelson SC WG TBWFD: 6-10% @ 24hr • Phase IV-Growout Water Temperature 23ºC Density: 70kg/m³ TBWFD: 1.5-2.0% @ 24hr Feed: Nelson SC WG Conclusion • NADF recycle system produced market size (avg. 454g) hybrid walleye in 316 days from fingerling (avg. 0.95g) at average tank densities of 34kg/m³. • Walleye and hybrid walleye can be successfully raised indoors using early advanced spawning, incubation, and rearing techniques. Conclusion • Initial data suggests that growth rates of hybrid walleye are higher than purebred walleye. • Processing yields of >50% and initial marketing studies indicate good aquaculture potential and high demand for hybrid walleye as food fish. Acknowledgements We would like to thank Alan Johnson at the Rathbun Fisheries Research Center in Iowa for all his assistance and guidance. We would also like to acknowledge the efforts of NADF technicians Lance Bresette, Nate Martin. Dr. Chris Hartleb (Co-director) and Jim Held(Extension) have also provided assistance and guidance throughout this project. This project was supported by an Agricultural Development & Diversification grant through the Wisconsin Department of Agriculture, Trade and Consumer Protection. Mention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty and does not imply approval to the exclusion of other products that may be suitable. 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