Name: Alisa Biology 12 Unit 2 Organic Molecules Worksheet Instructions: Please refer to the Unit 2 Assignment activity and answer in complete sentences. Carbohydrates: 1. What are monosaccharides and what molecules are contain in a monosaccharide (draw a diagram)? -Monosaccharides are single sugar molecules that include glucose, fructose, and galactose that react with Benedicts reagent to test presents of simple sugars. 2. What colour is Benedict’s reagent? What observations (colour and changes) were made when Benedict’s reagent was added to water? - Benedict reagent is dark blue. When added to water, solution turned blue. 3. What observations (colour and changes) were made when Benedict’s reagent was added to glucose solution and heated? - The Benedict solution changed to a think orange/red color. 4. Describe the expected results and observations for a positive test for simple sugar. Describe the expected results and observations for a negative test for simple sugar. - A positive test for simple sugar > Benedict reagent to turn orange/red. A negative test > Benedict reagent goes slightly light blue. 5. Describe the expected results and observations for a positive test for complex sugar (ie. Starch). Describe the expected results and observations for a negative test for complex sugar. What is the indicator used for testing complex sugar? - Negative results with added starch: Dark brown - Positive results with added starch: Yellow 6. If hydrolysis occurred with the sucrose, what would you expect to see if you did the Benedict’s test? Explain why. - If hydrolysis occurred the Benedicts reagent would remain a light blue color. 7. Would you do the iodine test? Explain why or why not. ____________________________________________________________________ ________ ____________________________________________________________________ ________ Proteins: 8. Describe the expected results and observations for a positive test for protein. Describe the expected results and observations for a negative test for protein. What is the indicator used for testing protein? - Expected positive results: Color turns from a light blue to violet. Protein reacts with blue reagent - Expected negative results: color will turn a dark green. Fats: 9. Describe the expected results and observations for a positive test for fat. Describe the expected results and observations for a negative test for fat. What is the indicator used for testing the presence of fat? - Expected results for positive test will change the color of fat to red. Negative test would result in half cloudy/clear and half red. Foods: 10. Complete the table of common food item and the presence of organic compounds. Please indicate a positive test (presence) with a “+” and a negative test with a “-.” Presence of Organic molecules Types of Food Potato Orange Juice Nuts (almonds) Eggs Salmon Milk Simple Sugars Starch Protein Fat + + + + - + + + + - + + + + - + + + -
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