EVERY CELL HAS A STORY TO TELL.™
FAST SINGLE CELL COPY NUMBER PROFILING
Genome-wide profiling of copy-number alterations (CNA) in single
cells enables the characterization and investigation of genomic
heterogeneity and evolution in tumor cell populations such as individual
circulating tumor cells (CTCs). Ampli1™ LowPass Kit is a rapid, highthroughput low-pass Whole Genome Sequencing (WGS) Kit for Copy
Number Alteration analysis on single cells.
Get a High Quality Single Cell Library in 6 Hours!
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LOW-PASS WGS LIBRARY PREP KIT FOR ION TORRENT™ PLATFORMS
FAST SINGLE CELL COPY NUMBER PROFILING
Blood
Collection
CTCs Enrichment
& Staining
DEPArray Sorting
100%
Pure Cell
Whole Genome
Amplification
Low-Pass WGS
Library Prep
Molecular
Characterization
Sequencing
By exploiting the deterministic nature of the Ampli1™ WGA Kit, the Ampli1™ LowPass Kit provides a precise snapshot
of cancer cell genome, drastically reducing the amount of bench work and cost. The accuracy of this method has been
assessed by comparing the copy number profiles generated by our approach with those obtained by the array CGH method.*
Method performance has been extensively validated by analyzing the copy number profiles of single CTCs and WBCs from
cancer patients (see figure below for representative data).
CTC
WBC
Whole genome profile (chr 1-22) of a single CTC and a single WBC isolated with DEPArray Technology from a patient with lung adenocarcinoma.
Ploidy values are indicated in the y-axis; in the x-axis, the alternation of different chromosomes are plotted with different colors. Copy number alterations
in the CTC are indicated in red and blue.
Ampli1™ LowPass Features:
• Accurate characterization of CNA profiles of multiple single tumor cells per single run
• Fast results (6h turnaround time for library preparation)
• Affordable cost for massively parallel single-cell analysis, such as multiple tumor cells or CTCs
CHOOSE AMPLI1™ LOWPASS FOR:
FAST SINGLE CELL COPY NUMBER PROFILING
Learn More at
www.siliconbiosystems.com/ampli1-wga-kit
*Buson G et al, AACR 2016, “Scalable, rapid and affordable low-pass whole genome
sequencing method for single-cell copy-number profiling on LM-PCR based WGA products”
©2016 Menarini Silicon Biosystems S.p.A. All rights reserved
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