NUCLEOTIDES, NUCLEOSIDES
HPLC
Biochemical: Nucleotides, Nucleosides and Nitrogenous Bases on Brownlee Validated IBD
Column: Brownlee Validated IBD
Peak List
Concentration (µg/mL)
Conditions
1. CDP
2. CMP
3. ATP
4. ADP
5. cytidine
6. TMP
7. AMP
8. guanine
9. guanosine
10. adenine
11. adenosine
146
113
117
145
155
157
125
36
103
38
115
Mobile phase
Flow
Temperature
Detector
Column
Column name
Part number
Dimensions
Particle size
Pore size
20 mM ammonium acetate, pH 5.8:
methanol (97.5:2.5)
1.0 mL/min
35 ˚C
UV @ 260 nm
Sample
Injection volume 20 µL
Solvent
20 mM ammonium acetate, pH 5.8
Notes
Recommended Guard Cartridge
Recommended Guard Holder
Brownlee Validated IBD
N9303582
150 x 4.6 mm
5 µm
100Å
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007568A_11
N9303702
N9303713