Name ________________________________________________
Date _________
Unit 3: Protein Enzyme Activity
Biology 2015
Protein Enzyme Demo 1: Cracker demo—put a piece of cracker on your tongue-don’t chew or swallow
for a minute or two. Write down the chemical formulas in parenthesis and label the reactants and the
products underneath:
Starch (_________)
Maltose sugar (______)
________________________
________________________________
1. Described what happened as you let the cracker “sit” in your mouth?
2. Which enzyme broke down the starches to produce a bland sugar?
3. Use the equation for starch breakdown to figure out how to label the substrate/reactant and
products—what is the name of the enzyme in saliva that catalyzes this reaction?
Demo 2: Hydrogen peroxide (H2O2) decomposes into water and oxygen gas, but normally the reaction is
so slow as to be imperceptible.
2H2O2
______________
2H2O + O2 gas
_________________________
Fill in: Adding the ______________ enzyme __________________ to hydrogen peroxide:
Observations
1. What happened during the reaction and how long did it take?
2. When more hydrogen peroxide was added to the same enzyme catalase what happened? Did
the reaction change?
Explanation: What happens when you pour hydrogen peroxide onto a cut? It bubbles! That's because
there are protein enzymes in your bodily fluids that catalyze or speed up the decomposition. A catalyst
is a substance that speeds up a reaction, without being consumed itself. In this experiment we use a 3%
Name ________________________________________________
Date _________
Unit 3: Protein Enzyme Activity
Biology 2015
hydrogen peroxide solution as the reactant. The production of oxygen gas created the bubbles that were
observed. The reaction is catalyzed by the catalase enzyme.
1. Use the equation for hydrogen peroxide breakdown to figure out how to label the
substrate/reactant and products—what is the name of the enzyme that catalyzes this reaction?
Enzymes
A type of _____________
made of ____________
______________________
and the names usually end
in “_________”
A type of chemical catalyst,
that:
Examples of enzyme action
are:
Name ________________________________________________
Date _________
Unit 3: Protein Enzyme Activity
Biology 2015
Which bodily functions are affected
by protein enzymes? List the
functions next to each body part:
Name ________________________________________________
Date _________
Unit 3: Protein Enzyme Activity
Biology 2015
Solve the puzzle! Materials: template (below), biology book page p. 56, glue, scissors, and a sheet of
white or binder paper.
Procedure:
1. Use different colors for the enzyme, substrate, and products. Cut out your pieces
2. Glue these onto white or binder paper using page 56 as a guide.
3. Title. Label the parts according to the figure on page 56 .
4. Explain the “lock and key” model of enzyme action on the same piece of paper.
Rubric (15 points)

Pictures are correctly and neatly placed
_______ (5pts)

Pictures are correctly titled, labeled and colored
_______ (5pts)

Explanation of enzyme lock and key
_______(5pts)
Name ________________________________________________
Date _________
Unit 3: Protein Enzyme Activity
Biology 2015