Directions for Use
Rapid RNA Gel Running Buffer, 10X
Code
Description
Size
1B1373-500ML
Rapid RNA Gel Running Buffer, 10X
500 mL
General Information
VWR Life Science AMRESCO’s Rapid RNA Gel Running Buffer, 10X is a direct replacement for
MOPS buffer, which is typically used in denaturing formaldehyde RNA gels. It is specially
formulated to allow application of higher voltages during electrophoresis without causing
overheating or gel distortion. RNA can be resolved twice as fast without adding any additional
protocol steps or new equipment purchases.


Faster gel results
Easy-to-use replacement for standard MOPS buffer
Storage/Stability
Store at room temperature (18 to 26°C).
Product Use Limitations
For research use only. Not for therapeutic or diagnostic use.
AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139
Directions for Use
Protocol/Procedure
Caution: Formaldehyde is toxic. Prepare formaldehyde gels in a fume hood to avoid inhalation
and wear appropriate protective equipment.
Preparation of Formaldehyde RNA Gel (100 mL)
1. Suspend agarose (1 – 2%) in 88.2 mL deionized water in a 250 mL conical flask.
2. In a microwave oven, heat the above mixture to a boil until the agarose has dissolved
completely. USE CAUTION, MIXTURE IS EXTREMELY HOT!
3. Let solution cool to 60 – 70°C and then add 10 mL of Rapid RNA Gel Running Buffer,
10X and mix thoroughly.
4. Carefully add 1.8 mL of 37% formaldehyde and mix thoroughly.
5. Pour the melted agarose into a horizontal agarose gel casting unit and insert comb.
6. Prepare 1X Rapid RNA Gel Running Buffer by diluting 1 part Rapid RNA Gel Running
Buffer, 10X with 9 parts nuclease-free water.
7. After the gel has completely solidified, remove the comb and completely submerge the
gel in 1X Rapid RNA Gel Running Buffer.
8. Mix RNA samples with RNA loading buffer.
9. Heat denature samples for 10 minutes at 65°C.
10. Load samples into wells and run gel in 1X Rapid RNA Gel Running Buffer at 15 – 18
V/cm until sufficient separation has been achieved.
AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139
Directions for Use
Frequently Asked Questions
Questions
Answers
Why do I see smears/or no
RNA on my gel?
1. RNase contamination in samples, prepare fresh RNA.
2. Smears may result from overloading, load < 30 µg RNA.
3. No RNA – not enough sample loaded or impure RNA is not
quantitated correctly.
4. No RNA – diffusion, image immediately after electrophoresis.
5. RNA ran off gel, use shorter run time.
6. Insufficient staining.
Is a gel run with Rapid
RNA Gel Running Buffer
compatible with Northern
Blotting?
Why didn’t my RNA leave
the loading well?
Yes
1. Improper well formation. Pour a new gel and repeat.
2. Overloaded RNA. Load < 30 ug RNA.
For Technical Support
Toll Free: 1-800-610-2789 (USA & Canada)
Fax: (440) 349-0235
Email: [email protected]
AMRESCO, LLC
A VWR Company
Corporate Headquarters
28600 Fountain Parkway
Solon, Ohio USA 44139-4300
Tel: 440/349-1199
Fax: 440/349-1182
www.amresco-inc.com
Rapid RNA Gel Running Buffer, 10X
ZY0697
Rev. 1 01/2016
© Copyright 2010 by AMRESCO, LLC
All Rights Reserved.
AMRESCO® is a registered trademark of AMRESCO, LLC
AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139