15th Day of Microscopy 2016
Workshop 8
From 3D Light Microscopy to 3D Electron Microscopy
Approaches and Tools for Correlative Experiments
The electron microscope (EM) is an invaluable tool for unravelling the structural details
of cells and tissue. For the majority of questions the transmission electron microscope
(TEM) is used to investigate resin embedded or cryo specimens. The scanning electron
microscope (SEM) is mainly used to collect information on the topography of a specimen.
It has recently been shown that the SEM can be used as an imaging tool for traditional
EM samples and thin sections and has some advantages over classical TEM approaches.
These advantages include the opportunity to section a resin embedded sample directly in
the SEM, prepare a range of serial sections and image them automatically and also include
correlative light and electron microscopy easily into the workflow. These advantages have the
potential to complement, or even replace, the TEM in a range of biological applications.
In this workshop we would like to discuss and demonstrate the applications of SEM for
bioscience:
• Overview of modern SEM applications for the biosciences
(e.g. SEM, FIB-SEM, 3View)
• Demonstration of Serial Section Acquisition
(i.e. Array Tomography) with the SEM
• Correlative Light and Electron Microscopy Methods
Carl Zeiss Microscopy GmbH
Jena, September 2016