Take a 96 well plate and divide up into a 24 well pH/PEG screen, a 24 well PEG/Cation screen and a 48 well
PEG/anion screen. Use different PEGs for the different subscreens
PEG/pH
(24)
PEG/ Cation
(24)
PEG/Anion (48)
This is the PEG/pH screen.
All wells have 25% PEG 1500
Succinic acid, Phosphate,
glycine system
Malonic acid, imidazol, boric
system
Propionic acid, cacodylate, BisTris Propane system
Malic, MES, Tris system
4
5
6
7
8
9
pH of the buffer system
This is the PEG/cation screen
All wells have 20% PEG 6000
Acetate pH 5
MES pH 6
HEPES pH 7
Tris pH 8
NaCl
NH4Cl
LiCl
MgCl2
CaCl2
ZnCl2
200mM of all, except for 10mM ZnCl2,
This is the PEG/anion screen
All wells have 20% PEG 3350
No Buffer
pH 6.5
pH 7.5
pH 8.5
NaF
NaBr
NaI
KSCN
NaNO3
NaFormate NaAcetate
Na2SO4 Na/Ktartrate Na/KPO4 NaCitrate NaMalonate
200mM of each
100mM
Bis-tris
propane
Chemical
Stock
pH
# Additions Total volume needed
Water
PEG 1500
PEG 3350
PEG 6000
50%
50%
50%
4
10
4
10
4
9.5
4
9
5
6
7
8
6.5
7.5
8.5
96
32.4
24
48
24
12
19.2
9.6
6
6
6
6
6
6
6
6
6
6
6
6
12
12
12
0.35
0.25
0.35
0.25
0.37
0.23
0.3
0.3
0.6
0.6
0.6
0.6
1.2
1.2
1.2
SPG system
SPG system
MIB system
MIB system
PCB system
PCB system
MMT system
MMT system
NaAcetate
MES
HEPES
Tris
Bis-Tris Propane
Bis-Tris Propane
Bis-Tris Propane
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
NaCl
NH4Cl
LiCl
MgCl2
CaCl2
ZnCl2
NaF
NaBr
NaI
KSCN
NaNO3
NaFormate
NaAcetate
NaAcetate
Na2SO4
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
4
4
4
4
4
4
4
4
4
4
4
4
4
0.8
0.8
0.8
0.8
0.8
0.04
0.8
0.8
0.8
0.8
0.8
0.8
0.8
1M
4
0.8
Na/K Tartrate
Na/K PO4
1M
1M
4
4
0.8
0.8
Trisodium Citrate
NaMalonate
1M
1M
4
4
0.8
0.8
7
Formula
MW
NaAcetate anhydrous
MES.H2O
HEPES
Tris
Bis-tris-propane
82.03
213.25
238.3
121.2
282.3
NaCl
NH4Cl
LiCl
MgCl2.6H2O
CaCl2.2H2O
ZnCl2
NaF
NaBr
NaI
KSCN
NaNO3
NaFormate
NaAcetate
NaAcetate.3H2O
Na2SO4.
Na2SO4.10H2O
K/Na tartrate.4H2O
Na2HPO4.2H20
NaH2PO4.H2O
K2HPO4.3H2O
KH2PO4
NaCitrate.2H2O
C3H2Na2O4.H2O
g/50ml
58.44
53.49
42.39
203.3
147.02
136.28
41.99
102.9
149.9
97.18
84.99
68.01
82.03
136.08
142
322.2
282.08
177.99
137.99
228.23
136.09
294.1
166
1M Sodium acetate buffer pH 5 - dissolve 4.1g of NaC2H302 anhydrous in 50ml H20, add about 26ml 1M acetic acid until pH = 5
1M Acetic acid: 5.75ml of glacial (17.4M) acetic acid made up to 100ml with water
1M MES pH 6 - dissolve 10.66g MES.H2O in 25ml H20 - add NaOH to pH 6(about 2.07ml 10M), and adjust final volume to 50ml
1M HEPES pH 7 - dissolve 11.92g HEPES in 25ml H20 - add NaOH until pH 7 (about 1.1ml 10M), adjust volume to 50ml
1M Tris pH 8 - dissolve up 6.06g tris add HCl (about 2.75ml 37%) to pH 8, adjust volume to 50ml
1M BisTrisPropane pH 8.5 - dissolve up 14.12g in 25ml H2O - add HCl to pH 8.5, adjust volume to 50ml
1M BisTrisPropane pH 6.5 - dissolve up 14.12g in 25ml H2O - add HCl to pH 6.5, adjust volume to 50ml
1M BisTrisPropane pH 7.5 - dissolve up 14.12g in 25ml H2O - add HCl to pH 7.5, adjust volume to 50ml
1M NaCl - dissolve up 2.92g NaCl in 50ml H20
1M NH4Cl -dissolve up 2.67g NH4Cl in 50ml H20
1M LiCl - dissolve up 2.12g LiCl in 50ml H2O
1M MgCl2 - dissolve up 10.17g of MgCl2.6H20 in 50ml H20
1M CaCl2 - dissolve up 7.35g of CaCl2.2H20 in 50ml H20
1M ZnCl2 - dissolve up 6.82g of ZnCl2 in 50ml H20
1M NaF - dissolve up 2.1g of NaF in 50ml H2O (note 1M is just on the edge of the limit of solubility of NaF)
1M NaBr - dissolve up 5.15g of NaBr in 50ml H2O
1M NaI - dissolve up 7.5g of NaI in 50ml H2O
1M KSCN - dissolve up 4.86g in 50ml H2O
1M NaNO3 - dissolve up 4.25g in 50ml H2O
1M NaFormate - dissolve up 3.4g NaCHO2 in 50ml H2O
1M NaAcetate - dissove up 4.1g of NaC2H3O2 anhydrous in 50ml H2O
1M Na2SO4 - dissolve up 7.1g of Na2SO4 anhydrous in 50ml H2O
1M Na/K Tartrate - dissolve 14.1g Na/K tartrate in 50ml H2O
1M Na/K PO4 - dissolve 2.22g Na2HPO4, 1.72g NaH2PO4, 2.85g K2HPO4, 1.7g KH2PO4 in 50ml H2O
1M Trisodium Citrate - dissolve 14.7g Na3Cit in 50ml H2O
1M NaMalonate - dissolve 8.3g in 50ml H2O - check pH is close to neutral
Notes: Na/K PO4 made up by taking 1M Na2HPO4, NaH2PO4, K2HPO4 KH2PO4 and mixing them together in the ratio of 1:1:1:1
20.000 0.200 M
21.000 0.200 M
22.000 0.200 M
23.000 0.200 M
24.000
25.000
26.000
27.000
28.000
29.000
30.000
31.000
32.000 0.200 M
33.000 0.200 M
34.000 0.200 M
35.000 0.200 M
36.000
37.000
38.000
39.000
40.000
41.000
42.000
43.000
44.000 0.200 M
45.000 0.200 M
46.000 0.200 M
47.000 0.200 M
48.000
49.000
50.000
51.000
52.000
53.000
54.000
55.000
56.000
57.000
58.000
59.000
60.000
61.000
62.000
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
0.010
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.010
0.100
0.080
0.060
0.040
0.020
0.000
0.200
0.010
0.100
0.082
0.064
0.046
0.028
0.010
0.200
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
12.000
13.000
14.000
15.000
16.000
17.000
18.000
19.000
0.010
0.100
0.083
0.067
0.050
0.033
0.017
0.200
11.000 0.200 M
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
Salt
SPG
SPG
SPG
SPG
SPG
SPG
NaCl
NH4Cl
LiCl
MgCl2
ZnCl2
1.000
1.000
1.000
1.000
0.100 M
0.100 M
NaF
NaBr
NaI
KCN
KNO3
NaFormate
NaAcetate
NaSulfate
Na/Ktartrate
Na/Kphosphate
NaCitrate
NaMalonate
NaF
0.100 M
NaBr
0.100 M
0.010
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
ZnCl2
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
M
M
M
M
M
M
M
M
0.100 M
0.100
0.000
0.020
0.040
0.060
0.080
0.100
0.100
1.000
1.000
1.000
0.100 M
0.100 M
0.100 M
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
M
M
M
M
M
M
M
M
0.100 M
0.100
0.000
0.018
0.036
0.054
0.072
0.090
0.100
1.000
1.000
1.000
0.100 M
0.100 M
0.100 M
1.000
1.000
1.000
4.00
4.00
4.00
4.00
4.00
4.00
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
0.100 M
0.100 M
0.100 M
ZnCl2
4.00
4.00
4.00
4.00
4.00
4.00
4.00
4.00
4.00
4.00
4.00
4.00
1.000
0.100
0.000
0.017
0.033
0.050
0.067
0.083
0.100
M
M
M
M
M
M
M
M
0.100 M
Buffer
SPG
SPG
SPG
SPG
SPG
SPG
Acetate
Acetate
Acetate
Acetate
Acetate
MIB
MIB
MIB
MIB
MIB
MIB
MES
MES
PCB
PCB
PCB
PCB
PCB
PCB
HEPES
HEPES
MMT
MMT
MMT
MMT
MMT
MMT
TRIS
8.000
8.000
8.000
8.000
8.000
7.000
9.000
9.000
9.000
9.000
9.000
9.000
8.000
7.000
7.000
7.000
7.000
6.000
9.500
9.500
9.500
9.500
9.500
9.500
7.000
6.000
6.000
6.000
6.000
5.000
10.000
10.000
10.000
10.000
10.000
10.000
6.000
5.000
[Precipitant 1] units
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
20.000 %
20.000 %
20.000 %
20.000 %
20.000
25.000
25.000
25.000
25.000
25.000
25.000
20.000
20.000 %
20.000 %
20.000 %
20.000 %
20.000
25.000
25.000
25.000
25.000
25.000
25.000
20.000
20.000 %
20.000 %
20.000 %
20.000 %
20.000
25.000
25.000
25.000
25.000
25.000
25.000
20.000
20.000 %
pH
[Precipitant 1]
10.000
25.000
10.000
25.000
10.000
25.000
10.000
25.000
10.000
25.000
10.000
25.000
5.000
20.000
5.000
20.000
5.000
20.000
5.000
20.000
0.100 BisTrisPro 6.500
0.100 BisTrisPro 6.500
0.100 TRIS
0.100 TRIS
0.100 TRIS
0.100 TRIS
0.100 TRIS
0.100
0.000
0.020
0.040
0.060
0.080
0.100
0.100
0.100 HEPES
0.100 HEPES
0.100 HEPES
0.100 HEPES
0.100
0.000
0.018
0.036
0.054
0.072
0.090
0.100
0.100 MES
0.100 MES
0.100 MES
0.100 MES
0.100
0.000
0.017
0.033
0.050
0.067
0.083
0.100
0.100 Acetate
pH [Buffer] [Buffer] units [stock] ml buffer in 1 ml
4.00 0.000 M
1.000
0.000
4.00 0.017 M
1.000
0.017
4.00 0.033 M
1.000
0.033
4.00 0.050 M
1.000
0.050
4.00 0.067 M
1.000
0.067
4.00 0.083 M
1.000
0.083
0.100 M
1.000
0.100
0.100 M
1.000
0.100
0.100 M
1.000
0.100
0.100 M
1.000
0.100
MMT
MMT
MMT
MMT
MMT
MMT
NaCl
0.200 NH4Cl
0.200 LiCl
0.200 MgCl2
0.200 CaCl2
0.010
0.100
0.080
0.060
0.040
0.020
0.000
0.200
PCB
PCB
PCB
PCB
PCB
PCB
NaCl
NH
0.200
4Cl
0.200 LiCl
0.200 MgCl2
0.200 CaCl2
0.010
0.100
0.082
0.064
0.046
0.028
0.010
0.200
MIB
MIB
MIB
MIB
MIB
MIB
NaCl
NH
0.200
4Cl
0.200 LiCl
0.200 MgCl2
0.200 CaCl2
0.100
0.083
0.067
0.050
0.033
0.017
0.200
0.200 CaCl2
0.010 ZnCl2
[Salt] units [stock] ml salt in 1ml
M
1.000
0.100
M
1.000
0.083
M
1.000
0.067
M
1.000
0.050
M
1.000
0.033
M
1.000
0.017
M
1.000
0.200
M
1.000
0.200
M
1.000
0.200
M
1.000
0.200
A12
B1
B2
B3
B4
B5
B6
B7
[Salt]
0.100
0.083
0.067
0.050
0.033
0.017
0.200
0.200
0.200
0.200
A11
Reagent position reagent number
A1
1.000
A2
2.000
A3
3.000
A4
4.000
A5
5.000
A6
6.000
A7
7.000
A8
8.000
A9
9.000
A10
10.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
PEG 6K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 6K
PEG 6K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 6K
PEG 6K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 1.5K
PEG 6K
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
PEG 6K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
0.400 PEG 6K
0.400 PEG 6K
0.400 PEG 6K
0.400 PEG 6K
0.400
0.500
0.500
0.500
0.500
0.500
0.500
0.400
0.400 PEG 6K
0.400 PEG 6K
0.400 PEG 6K
0.400 PEG 6K
0.400
0.500
0.500
0.500
0.500
0.500
0.500
0.400
0.400 PEG 6K
0.400 PEG 6K
0.400 PEG 6K
0.400 PEG 6K
0.400
0.500
0.500
0.500
0.500
0.500
0.500
0.400
0.490
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.300
0.300
0.300
0.300
0.300
0.300
0.490
0.400
0.400
0.400
0.400
0.400
0.400
0.300
0.300
0.300
0.300
0.300
0.490
0.400
0.400
0.400
0.400
0.400
0.400
0.300
0.300
0.300
0.300
0.300
0.490
0.400
0.400
0.400
0.400
0.400
0.400
0.300
0.300
Precipitant 1 ml H2O in 1 ml
PEG 1.5K
0.400
PEG 1.5K
0.400
PEG 1.5K
0.400
PEG 1.5K
0.400
PEG 1.5K
0.400
PEG 1.5K
0.400
PEG 6K
0.300
PEG 6K
0.300
PEG 6K
0.300
PEG 6K
0.300
0.400 PEG 6K
[stock] ml precipitant in 1ml
50.000
0.500
50.000
0.500
50.000
0.500
50.000
0.500
50.000
0.500
50.000
0.500
50.000
0.400
50.000
0.400
50.000
0.400
50.000
0.400
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
63.000
64.000
65.000
66.000
67.000
68.000
69.000
70.000
71.000
72.000
73.000
74.000
75.000
76.000
77.000
78.000
79.000
80.000
81.000
82.000
83.000
84.000
85.000
86.000
87.000
88.000
89.000
90.000
91.000
92.000
93.000
94.000
95.000
96.000
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
NaI
KCN
KNO3
NaFormate
NaAcetate
NaSulfate
Na/Ktartrate
Na/Kphospha
NaCitrate
NaMalonate
NaF
NaBr
NaI
KCN
KNO3
NaFormate
NaAcetate
NaSulfate
Na/Ktartrate
Na/Kphospha
NaCitrate
NaMalonate
NaF
NaBr
NaI
KCN
KNO3
NaFormate
NaAcetate
NaSulfate
Na/Ktartrate
Na/Kphospha
NaCitrate
NaMalonate
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
0.100
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
BisTrisPro
6.500
6.500
6.500
6.500
6.500
6.500
6.500
6.500
6.500
6.500
7.500
7.500
7.500
7.500
7.500
7.500
7.500
7.500
7.500
7.500
7.500
7.500
8.500
8.500
8.500
8.500
8.500
8.500
8.500
8.500
8.500
8.500
8.500
8.500
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
20.000
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
50.000
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
0.400
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
PEG 3.35K
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
0.300
192.43
238.3
207.3
166
68.08
61.83
96.06
214.03
282.3
82.03
190.2
163.18
134.1
195.2
121.1
230.1
209.24
132.1
Citric Acid anhydrous (Fluka 27487)
HEPES (Merck 1.10110)
CHES (Sigma C2885)
Sodium Malonate (Sigma M1875)
Imidazol (Fluka 56750)
Boric Acid (Sigma B9645)
Sodium Propionate (Sigma P1880)
Sodium Cacodylate trihydrate (Fluka 20838)
Bis-Tris Propane (Sigma B6755)
Sodium Acetate (Merck 1.06268)
ADA (Hampton Research 0.5M Stock)
Bicine (Fluka 14872)
DL- Malic Acid (Sigma M1000)
MES (Sigma M8250)
Tris (Trizma Base,Sigma T1503)
Sodium Tartrate dihydrate (Sigma S4797)
Bis-Tris (Aldrich 15,666-3)
GlycylGlycine (Sigma G1002)
2
3
4
2
3
3
2
1
2
1
1
1
1
2
2
3
2
2
Citric Acid, HEPES, CHES (CHC)
Malonic Acid, Imidazol, Boric Acid
(MIB)
Sodium Propionate, Sodium
Cacodylate, Bis-Tris Propane
(PCB)
Sodium Acetate, ADA, Bicine
(AAB)
(MMT)
Sodium Tartrate, Bis-Tris, GlycylGlycine
(TBG)
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
1M
Concentration
100ml
100ml
100ml
100ml
Final volume
9.86
5.98
3.77
2.68
7.81
5.22
100ml
100ml
100ml, of
2.74
6.34 which 66.7ml
5.44 are 0.5M ADA
3.84
4.28
11.29
4.15
2.55
2.32
4.28
7.94
9.21
1.48
6.04
3.28
g
2.0 ml
NaOH
(pH 4.0)
6.3 ml
HCl
(pH 4.0)
13.5ml
NaOH
(pH 10.0)
3.5 ml
NaOH
(pH 10.0)
2.95 ml
HCl
(pH 4)
2.55ml
HCl
(pH 4.0)
5.1 ml
HCl
(pH 4.0)
5.7ml
NaOH
(pH 9)
6.7ml
NaOH
(pH 9)
2.4 ml
NaOH
(pH 9.0)
12.9 ml
HCl
(pH 4.0)
0.29ml
NaOH
(pH 4.0)
8.3ml
NaOH
(pH 10.0)
1.0 ml
HCl
(pH 9.5)
Low pH
High pH
Set the pH to either 4 or the upper limit using concentrated (32% = 10M) HCl or 10M NaOH. Numbers under column High pH or Low pH give approximate volumes of concentrated
acid or base to pH 100ml 1M buffer.
Malic Acid, MES, Tris
118.1
137.99
75.07
Succinic Acid (Sigma S3674)
Sodium Dihydrogen Phosphate monohydrate (Merck 1.06346)
Glycine (Merck 1.04201)
2
7
7
Formula Weight
Succinic Acid, Sodium Dihydrogen
Phosphate, Glycine (SPG)
Chemical Name and source
Ratio
System name
Scoring sheet
1
2
0.2M NH4Cl
pH 6.3
20% PEG
3350
3
5
6
7
Na/K PO4 pH
0.2M NaCl
0.2M MgCl2 1M NaCit
6.2
Phos-cit pH
Hepes pH 7.5 Cacodylate
25% 1,2
4.2
pH 6.5
Propanediol 70% MPD
20% PEG 8K
10% glycerol
A
0.2M Li2SO4
Acetate pH
4.5
50% PEG 400
B
0.2M KFo pH
Citrate pH 5.5 7.3
20% PEG 3K 20% PEG
3350
Phos-cit pH
1M LiCl
4.2
Citrate pH 4
40% Ethanol
20% PEG 6K
5% PEG 1K
C
0.2M AmCit
pH 5
20% PEG
3350
Acetate pH
4.6
8% PEG 4K
D
0.2M KNO3
20mM CaCl2
pH 6.9
NaAc. pH 4.6
20% PEG
30% MPD
3350
E
0.2M MgFo
pH 5.9
20% PEG
3350
0.8M
0.17M
NaH2PO4
24% PEG
AmSO4
CAPS pH
0.8M AmSO4 Citrate pH 5
0.8M
1.5K
25.5% PEG 10.5
Citrate pH 4 20% PEG 6K
KH2PO4
20% glycerol 4K
40% MPD
Hepes pH 7.5
15% glycerol
F
0.2M Li2SO4
Phos-cit pH
4.2
20% PEG 1K
0.2M NaSCN
pH 6.9
20% PEG
3350
G
CHES pH 9.5 Bicine pH 9
1.6M NaCit
20% PEG 8K 20% PEG 6K
H
0.2M AmFo
pH 6.6
20% PEG
3350
0.2M
NH4H2PO4
Tris pH 8.5
50% MPD
Hepes pH 7.5
10% PEG 8K
8% Ethylene
Glycol
Cacodylate
pH 6.5
40% MPD
5% PEG 8K
4
0.2M AmNit.
pH 6.3
20% PEG
3350
8
9
0.2M MgCl2
1.6M MgSO4 Tris pH 8.5
MES pH 6.5 50% ethylene
glycol
2M AmSO4
Tris pH 8.5
0.2M NaCl
20% PEG 8K Cacodylate
pH 6.5
2M AmSO4
Acetate ph
4.6
1.6M CaAc
0.8M
Cacodylate
0.2M Li2SO4 0.2M NaCl
succinate pH
pH 6.5
Tris pH 8.5
Hepes pH 7.5
14.4% PEG 7.0
40% PEG 400 10% propanol
8K
20% glycerol
0.2M MgCl2
Phos-cit pH 0.2M MgCl2
Cacodylate
4.2
Hepes pH 7.5
pH 6.5
40% PEG 300 30% PEG 400
50% PEG 200
0.2M ZnAc.
0.2M NaCl
Acetate pH
Na/K PO4 pH
4.5
10% PEG 3K 6.2
50% PEG 200
0.2M Kcit pH
0.2M Li2SO4
8.3
Tris pH 8.5
Acetate ph
20% PEG
20% Ethanol 4.5
3350
30% PEG 8K
11
Hepes pH 7.0
0.1M KSCN
30%
30% PEG
Jeffamine EDMME 2K
2001
0.02M MgCl2
Hepes pH 7.5 0.15M KBr
Bicine pH 9.0 Bicine pH 9.0
22%
30% PEG
10% PEG 6K 10% MPD
polyacrylic
MME 2K
acid 5100
Bicine pH 9
10% PEG
20K
2% dioxane
0.2M MgCl2
Hepes pH 7 10% PEG 1K Tris pH 8.0
Tris pH 7
10% PEG 6K 10% PEG 8K 40% MPD
10% PEG 8K
10
1.26M
AmSO4
Imidazol pH 8 2.1M DL
0.2M Li2SO4 10% PEG 8K malate pH 7.0
Tris pH 8.5
0.05M CsCl
MES pH 6.5 2.4M
30%
malonate pH
Jeffamine M- 7.0
600
12
0.2M AmAc
Bis-Tris pH
5.5
45% MPD
0.1M AmAc
Bis-Tris pH
5.5
17% PEG
10K
0.1M CoCl2
tris pH 8.5
2M AmSO4
20% polyvinyl Bis-Tris pH
pyrrolidone
5.5
K15
0.2M AmSO4
Bis-Tri pH 5.5
25% PEG
3350
0.2M TMAO
Tris pH 8.5
20% PEG
MME 2K
0.2M NaCl
Bis-Tris pH
5.5
25% PEG
3350
3M NaCl
Bis-Tris pH
5.5
5mM
CoCl2,CdCl2,
0.3M MgFo.
MgCl2,NiCl2
Bis-Tris pH
Hepes pH 7.5
5.5
12% PEG
3350
0.2M Li2SO4
Bis-Tris pH
5.5
25% PEG
3350
0.2M CaAc
0.2M ZnAc.
3.15M
Cacodylate
Imidazol pH 8 AmSO4
pH 6.5
20% PEG 3K Citrate pH 5.0
40% PEG 300
1.1M
malonate pH
7.0
Hepes pH 7.0
0.5%
Jeffamine ED-
0.2M
malonate pH
7.0
20% PEG
3350
0.2M AmAc
1M AmSO4
Bis-Tris pH
Bis-Tris pH
5.5
5.5
25% PEG
1% PEG 3350
3350
0.14M CaCl2
Acetate pH
4.6
14% propanol
30% glycerol
0.2M ZnAc.
Cacodylate
Tris pH 8.0
pH 6.5
20% MPD
10% propanol
1.0M
succinate
Hepes pH 7
1% PEG
MME 2K
0.1M
succinate pH
7.0
15% PEG
3350
Bis-Tris pH
5.5
25% PEG
3350
0.2M MgCl2
Bis-Tris pH
5.5
25% PEG
3350
0.04M
KH2PO4
16% PEG 8K
20% glycerol
1M
(NH4)2HPO4
Acetate pH
4.5
0.2M CaCl2
Bis-Tris pH
5.5
45% MPD
0.2M AmAc
Hepes pH
7.5
45% MPD
0.15M DLHepes pH 6.5
Hepes ph 7.0 malate pH 7.0
20%
30% jeffamine 20% PEG
Jeffamine MM-600 pH 7 3350
600
4
5
6
7
8
9
10
11
12
13
14
15
16
16
17
17
18
18
19
20
21
22
23
24
25
26
27
28
29
29
30
31
32
33
33
34
34
35
36
36
37
37
38
39
40
41
42
43
44
45
45
46
47
47
48
48
49
50
(NH4)CHO2 pH 6.6
(NH4)Cl pH 6.3
KCHO2 pH 7.3
(NH4)H2PO4
KNO3 pH 6.9
(NH4)2SO4
NaSCN pH 6.9
0.20
0.20
0.20
0.20
0.20
0.80
0.20
MgCl2
Na3C6H5O7
K3C6H5O7 pH 8.3
NaCl
LiCl
(NH4)NO3 pH 6.3
0.20
1.6
0.20
0.20
1.00
0.20
KH2PO4
Na3C6H5O7
(NH4)2SO4
0.04 M
1.00 M
2.00 M
Li2SO4
0.20 M
(NH4)2SO4
MgCl2
NaCl
Li2SO4
MgCl2
M
M
M
M
0.20
0.20
0.20
0.20
Ca(C2H3O2)2
CaCl2
(NH4)2SO4
2.00 M
0.17 M
Zn(C2H3O2)2
0.20 M
0.20 M
0.14 M
NaH2PO4
KH2PO4
0.80 M
0.80 M
M
M
M
M
M
M
MgCl2
0.20 M
M
M
M
M
M
M
M
CaCl2
Mg(CHO2)2 pH 5.9
Li2SO4
0.02 M
0.20 M
0.20 M
NaOAcetate
tris
citrate
cacodylate
0.1
0.1
0.1
0.1
phosphate-citrate
acetate
tris
Na/K phosphate
0.1
0.1
0.1
0.1
M
M
M
M
M
M
M
0.1 M
0.1 M
0.1 M
0.07 M
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1 M
0.1 M
cacodylate
cacodylate
cacodylate
acetate
HEPES
Na/K phosphate
acetate
HEPES
tris
tris
tris
acetate
bicine
HEPES
HEPES
0.1 M
0.1 M
M
M
M
M
phosphate-citrate
citrate
0.1 M
0.1 M
M
M
M
M
cacodylate
phosphate-citrate
0.1 M
bicine
HEPES
0.1 M
0.1 M
0.1 M
tris
citrate
0.1 M
phosphate-citrate
CHES
0.1 M
0.1 M
0.1 M
NaOAcetate
0.1 M
Reagent number [Salt1] [Salt1] units Salt 1
[Buffer] [Buffer] units Buffer
Li2SO4
0.1 M
acetate
1
0.20 M
2
0.1 M
citrate
(NH4)2HC6H5O7 pH 5.0
3
0.20 M
6.5
6.5
6.5
4.6
7.5
6.2
4.5
7.5
8.5
8.5
8.0
4.6
9.0
4.2
4.5
8.5
6.2
7.0
7.5
4.2
4.0
4.6
7.0
5.0
6.5
4.2
6.5
9.0
7.5
4.0
8.5
4.2
9.5
4.6
10
10
24
20
30
50
30
70
20
40
40
25.5
15
40
14
30
16
20
%w/v
%w/v
%w/v
%v/v
%v/v
%v/v
%w/v
%v/v
%w/v
%v/v
%v/v
%w/v
%v/v
%v/v
%v/v
%v/v
%w/v
%v/v
%v/v
%w/v
%v/v
%v/v
%v/v
%w/v
%v/v
%w/v
%w/v
%w/v
%w/v
%w/v
20
20
20
20
10
40
10
20
25
10
10
2
%w/v
%w/v
%w/v
%v/v
%v/v
%w/v
%v/v
%w/v
%w/v
%w/v
%w/v
%v/v
%v/v
%w/v
%w/v
%w/v
%w/v
%w/v
%w/v
%v/v
%w/v
20
20
10
8
40
5
40
5
8
10
20
50
30
20
20
20
20
20
20
50
20
50
50
50
100
100
100
50
100
50
100
100
50
100
100
100
100
50
100
100
50
100
100
100
30
40
50
50
50
50
50
50
50
50
100
100
50
100
50
50
50
50
100
100
50
50
50
50
50
50
100
50
0.200
0.200
0.480
0.200
0.300
0.500
0.600
0.700
0.400
0.400
0.400
0.510
0.150
0.400
0.140
0.300
0.320
0.200
0.400
0.200
0.200
0.250
0.100
0.333
0.050
0.400
0.400
0.400
0.400
0.200
0.400
0.400
0.200
0.080
0.400
0.100
0.400
0.100
0.160
0.200
0.400
0.500
0.300
0.400
0.400
0.400
0.400
0.400
0.400
0.500
0.400
pH [Precipitant 1] [Precipitant 1] units [Stock] ml stock in 1ml
4.5
50 %w/v
100
0.500
5.5
20 %w/v
50
0.400
20 %w/v
50
0.400
PEG 1000
PEG 8000
PEG 1500
glycerol
PEG 400
PEG 200
PEG 8000
MPD
PEG 8000
PEG 400
MPD
PEG 4000
glycerol
PEG 300
2-propanol
glycerol
PEG 8000
glycerol
PEG 300
PEG 3000
Ethanol
1,2 propandiol
glycerol
PEG 20000
dioxane
PEG 3350
PEG 8000
PEG 6000
PEG 3350
PEG 6000
PEG 3350
PEG 6000
PEG 8000
Ethylene glycol
MPD
PEG 8000
Ethanol
PEG 1000
PEG 4000
PEG 8000
PEG 6000
PEG 200
MPD
PEG 3350
PEG 1000
PEG 8000
PEG 3350
PEG 3350
PEG 3350
MPD
PEG 3350
Precipitant 1
PEG 400
PEG 3000
PEG 3350
0.580
0.400
0.400
0.500
0.580
0.560
0.586
0.320
0.533
0.671
0.575
0.500
0.620
0.080
0.400
0.100
0.400
0.100
0.740
0.600
0.500
0.300
0.000
0.520
0.460
0.400
0.580
0.700
0.207
0.533
0.500
0.500
0.700
0.550
0.100
0.517
0.050
0.329
0.600
0.200
0.320
0.200
0.500
0.360
0.200
0.100
0.500
0.400
0.500
0.291
0.150
0.300
0.350
0.300
0.453
0.200
0.275
0.289
4
5
6
7
8
9
10
11
12
13
14
15
16
16
17
17
18
18
19
20
21
22
23
24
25
26
27
28
29
29
30
31
32
33
33
34
34
35
36
36
37
37
38
39
40
41
42
43
44
45
45
46
47
47
48
48
49
50
ml H2O in 1ml Reagent number
0.300
1
0.500
2
0.520
3
W1 #14
W2 #04
H1cryo #42
W1cryo #37
H1cryo #24
H1cryo #23
W2cryo #15
W1 #17
H2 #35
W2 #03
W1cryo #38
MPD #17
H1cryo #31
H1cryo #43
H1 #47
H2 #07
H2 #48
W2cryo #18
W2 #01
H2 #44
W2cryo #11
H1 #37
W2 #43
P6K #14
W2cryo #36
H2 #28
Peg/Ion #47
W1 #31
P6K/LiCl #13
Peg/Ion #19
P6K #10
H1 #35
W1cryo #40
W2cryo #01
H1 #01
PEG/Ion #20
W1 #39
W1 #01
PEG/Ion #23
PEG/Ion #09
PEG/Ion #22
H2 #43
Peg/Ion #18
(NH4)2SO4 #01
Peg/Ion #13
P6K
H2 #37
source screen
W1 cryo #47
W1 #06
PEG/Ion #48
67
68
69
70
71
72
73
74
75
76
77
77
77
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
50
51
52
52
53
54
55
56
57
58
59
59
60
61
62
63
64
65
66
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
0.02
0.10
0.20
0.005
0.005
0.005
0.005
0.20
0.10
0.15
0.10
0.15
2
3
0.3
1.0
0.2
0.2
0.10
0.20
0.20
0.20
0.20
0.20
0.2
MgCl2
0.20 M
CaCl2
(NH4)(C2H3O2)
(NH4)(C2H3O2)
(NH4)2SO4
NaCl
Li2SO4
(NH4)(C2H3O2)
MgCl2
(NH4)(C2H3O2)
MgCl2
CoCl2
(CH3)3NO
CoCl2
CdCl2
MgCl2
NiCl2
C3H4O4 pH 7.0
C4H6O4 pH 7.0
C4H6O5 pH 7.0
KSCN
KBr
(NH4)2SO4
NaCl
Mg(CHO2)2
(NH4)2SO4
C4H6O4 pH 7.0
C4H6O5 pH 7.0
C3H4O4 pH 7.0
C3H4O4 pH 7.0
C4H6O4 pH 7.0
CsCl
(NH4)2SO4
0.05 M
3.15 M
M
M
M
M
M
Ca(C2H3O2)2
0.16 M
0.8
2.1
2.4
1.1
1.0
Zn(C2H3O2)2
Zn(C2H3O2)2
(NH4)2HPO4
MgSO4
M
M
M
M
0.20
0.20
1.00
1.60
NaCl
NaCl
(NH4)2SO4
Li2SO4
M
M
M
M
0.20
0.20
1.26
0.20
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
M
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
M
M
M
M
M
M
M
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
M
M
M
M
M
M
M
0.1
0.1
0.1
0.1
0.1
0.1
0.08
0.1 M
0.1 M
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
bis-tris
HEPES
HEPES
HEPES
HEPES
HEPES
HEPES
tris
tris
HEPES
imidazole
MES
citrate
tris
HEPES
tris
bicine
CAPS
imidazole
cacodylate
acetate
MES
Bicine
cacodylate
HEPES
tris
5.5
5.5
5.5
5.5
5.5
5.5
5.5
5.5
5.5
5.5
5.5
5.5
5.5
7.5
7.0
7.0
7.0
7.0
7.5
8.5
8.5
7.5
8.0
6.5
5.0
8.0
6.5
8.5
9.0
10.5
8.0
6.5
4.5
6.5
9.0
6.5
7.5
8.5
%w/v
%w/v
%v/v
%v/v
%w/v
%w/v
%w/v
%w/v
%w/v
%w/v
%v/v
%w/v
%w/v
%w/v
%w/v
%w/v
20
15
20
30
30
1
25
45
45
17
25
25
25
25
25
45
%v/v
%w/v
%v/v
%v/v
%w/v
%w/v
%w/v
%w/v
%v/v
%w/v
%v/v
%v/v
20
20
50
10
0.5
1
30
30
22
20
20
12
%w/v
%w/v
%v/v
%w/v
%w/v
10
14.4
20
10
30
40 %v/v
20 %w/v
10 %v/v
10 %v/v
50
50
100
100
50
50
50
50
50
50
100
50
50
50
50
50
50
50
50
50
50
50
50
50
100
50
100
100
50
50
100
50
50
100
50
100
100
0.02
0.5
0.45
0.45
0.34
0.5
0.5
0.5
0.5
0.5
0.45
0.4
0.3
0.4
0.6
0.6
0.01
0.02
0.6
0.6
0.44
0.4
0.4
0.24
0.200
0.400
0.500
0.100
0.200
0.288
0.200
0.200
0.600
PEG 3350
PEG 3350
MPD
MPD
PEG 10,000
PEG 3350
PEG 3350
PEG 3350
PEG 3350
PEG 3350
MPD
PEG 3350
PEG 3350
PEG 3350
PEG MME 2000
PEG MME 2000
jeffamine ED-2001 reagent pH 7.0
PEG MME 2000
jeffamine M-600 reagent pH 7.0
jeffamine ED-2001 reagent pH 7.0
polyacrylic acid 5100 sodium salt
polyvinylpyrrolidone K15
PEG MME 2000
PEG 3350
MPD
Jeffamine M-600
ethylene glycol
MPD
PEG 6000
PEG 8000
glycerol
PEG 8000
Jeffamine M-600
0.400 MPD
0.400 PEG 3000
0.100 2-propanol
0.100 2-propanol
0.040
0.760
0.440
0.100
0.500
0.300
0.600
0.614
0.260
0.700
0.272
0.200
0.700
0.250
0.000
0.700
0.500
0.300
0.800
0.333
0.300
0.294
0.566
0.047
0.300
0.300
0.450
0.400
0.300
0.646
0.005
0.003
0.001
0.541
0.617
0.550
0.388
0.250
0.329
0.300
0.600
0.594
0.400
0.250
0.250
0.460
0.343
0.360
0.300
0.200
0.300
0.250
67
68
69
70
71
72
73
74
75
76
77
77
77
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
50
51
52
52
53
54
55
56
57
58
59
59
60
61
62
63
64
65
66
Index H3
Index H5
Index H7
Index H11
Index H12
Index A3
Index A9
Index B1
Index C8
Index D6
Index D12
Index E2
Index F5
Index F6
Index F10
Index G2
Index G6
Index G10
Index E4
W2 #34
H2 #24
(NH4)2SO4 #20
MPD #11
H2 #31
W1cryo #43
MPD #06
Index B10
Index B11
Index C3
Index C9
Index C10
Index D2
Index D3
Index E11
Index E12
Index F2
Index F4
W2cryo #25
W2 #40
W2 #11
W1 #07
H2 #20
P6K #12
H1cryo #46
W1 #02
W1 #47
count
20
Chemical
acetate
acetate
bicine
bis-tris
cacodylate
CAPS
CHES
citrate
citrate
citrate
HEPES
HEPES
HEPES
imidazole
MES
Na/K phosphate
phosphate-citrate
tris
tris
tris
type
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
buffer
pH
Stock smallest volume/ 1ml largest volume/ 1ml # of additions ml to make up 1ml screen
4.5
1
0.1
0.1
4
0.4
4.6
1
0.07
0.1
4
0.37
9
1
0.1
0.1
4
0.4
5.5
1
0.1
0.1
13
1.3
6.5
1
0.08
0.1
7
0.68
10.5
1
0.1
0.1
1
0.1
9.5
1
0.1
0.1
1
0.1
4
1
0.1
0.1
2
0.2
5
1
0.1
0.1
2
0.2
5.5
1
0.1
0.1
1
0.1
6.5
1
0.1
0.1
1
0.1
7
1
0.1
0.1
5
0.5
7.5
1
0.1
0.1
8
0.8
8
1
0.1
0.1
2
0.2
6.5
1
0.1
0.1
2
0.2
6.2
1
0.1
0.1
2
0.2
4.2
1
0.1
0.1
4
0.4
7
1
0.1
0.1
1
0.1
8
1
0.1
0.1
2
0.2
8.5
1
0.1
0.1
8
0.8
7
1,2 propandiol
2-propanol
dioxane
Ethanol
Ethylene glycol
glycerol
MPD
organic
organic
organic
organic
organic
organic
organic
100
100
40
100
100
100
100
0.25
0.1
0.05
0.2
0.08
0.1
1
0.25
0.14
0.05
0.4
0.5
0.3
0.7
1
3
1
2
2
6
11
0.25
0.34
0.05
0.6
0.58
1.15
4.35
16
Jeffamine M-600
PEG 1000
PEG 10000
PEG 1500
PEG 200
PEG 20000
PEG 300
PEG 3000
PEG 3350
PEG 400
PEG 4000
PEG 6000
PEG 8000
PEG MME 2000
polyacrylic acid 5100 sodium salt
polyvinylpyrrolidone K15
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
polymer
50
50
50
50
100
30
100
50
50
100
50
50
50
50
50
50
0.4
0.1
0.34
0.48
0.5
0.333
0.4
0.2
0.02
0.3
0.16
0.2
0.2
0.02
0.44
0.4
0.6
0.4
0.34
0.48
0.5
0.333
0.4
0.4
0.5
0.5
0.51
0.4
0.6
0.6
0.44
0.4
3
3
1
1
2
1
2
3
20
3
2
5
11
4
1
1
1.6
0.7
0.35
0.48
1
0.333
0.8
1
7.96
1.2
0.67
1.6
3.308
1.62
0.44
0.4
34
(CH3)3NO (TMAO
(NH4)(C2H3O2)
(NH4)2HC6H5O7
(NH4)2HPO4
(NH4)2SO4
(NH4)CHO2
(NH4)Cl
(NH4)H2PO4
(NH4)NO3
C3H4O4 (sodium malonate)
C4H6O4 (sodium succinate)
C4H6O5 (DL-sodium malate)
Ca(C2H3O2)2
CaCl2
CdCl2
CoCl2
CsCl
K3C6H5O7
KBr
KCHO2
KH2PO4
KNO3
KSCN
Li2SO4
LiCl
Mg(CHO2)2
MgCl2
MgSO4
Na3C6H5O7
NaCl
NaH2PO4
NaSCN
NiCl2
Zn(C2H3O2)2
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
salt
1
1
2.5
3.5
3.5
10
5
2.5
10
3.4
1.2
3
1
1
1
1
1
2.5
1
14
1.5
3
8
2
10
1
2
2.5
1.6
5
5
8
4
1
0.2
0.1
0.08
0.286
0.049
0.02
0.04
0.08
0.02
0.059
0.083
0.05
0.16
0.02
0.005
0.005
0.05
0.08
0.15
0.014
0.027
0.067
0.013
0.1
0.1
0.2
0.003
0.64
0.625
0.04
0.16
0.025
0.001
0.2
0.2
0.2
0.08
0.286
0.9
0.02
0.04
0.08
0.2
0.706
0.833
0.7
0.2
0.2
0.005
0.1
0.05
0.08
0.15
0.014
0.533
0.067
0.013
0.1
0.1
0.3
0.1
0.64
1
0.6
0.16
0.025
0.001
0.2
1
4
1
1
9
1
1
1
1
3
3
2
2
3
1
2
1
1
1
1
2
1
1
6
1
2
8
1
2
6
1
1
1
3
0.2
0.7
0.8
0.286
3.594
0.02
0.04
0.08
0.02
1.088
1.583
0.75
0.36
0.36
0.005
0.105
0.05
0.08
0.15
0.014
0.56
0.067
0.013
0.6
0.1
0.5
0.613
0.64
1.625
0.8
0.16
0.025
0.001
0.6
5
6.6
6.3
6.3
7
7
7
8.3
7.3
6.9
5.9
6.9