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Mathematics of Data/Image Pattern Recognition, Compression, and Encryption with Applications XI, edited by
Mark S. Schmalz, Gerhard X. Ritter, Junior Barrera, Jaakko T. Astola, Proc. of SPIE Vol. 7075, 70750J, (2008)
0277-786X/08/$18 · doi: 10.1117/12.794348
Proc. of SPIE Vol. 7075 70750J-1
1000
Alexa488−FITC
400
Intensity
600
800
Mixture
200
Ideal curve
Alexa488
0
FITC
510
520
530
540
550
560
Wave length [nm]
, # - % 1 / 1 ' ' ' !()** !(234 / ' 5 . 5
5
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Proc. of SPIE Vol. 7075 70750J-2
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250
200
510
520
530
540
550
560
50
0
0
0
50
10
100
150
Intensity
30
Intensity
20
150
100
Intensity
200
40
250
50
300
Alexa532
300
Alexa488
510
520
Wave length [nm]
530
540
550
560
510
520
Wave length [nm]
530
540
550
560
Wave length [nm]
, + 1 ' J ' Alexa532
FITC
0.5
0.5
1.0
Intensity
1.0
Intensity
1.0
0.5
0.0
0.0
Intensity
1.5
1.5
1.5
2.0
2.0
Alexa488
0
2
4
6
Concentration [ug/ml]
8
0
2
4
6
8
Concentration [ug/ml]
, ' 9 , Proc. of SPIE Vol. 7075 70750J-6
0.0
0.1
0.2
0.3
Concentration [ug/ml]
0.4
Mixture
800
250
300
250
200
Intensity
150
Ideal curve
100
400
100
150
Intensity
Mixture
Mixture
600
200
Ideal curve
Intensity
Alexa532−FITC
300
Alexa488−FITC
1000
Alexa488−Alexa532
Ideal curve
Alexa488
50
50
200
FITC
Alexa488
FITC
510
520
530
540
550
0
Alexa532
0
0
Alexa532
560
510
520
530
Wave length [nm]
540
550
560
510
520
530
Wave length [nm]
540
550
560
Wave length [nm]
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8
6
4
Estimated Concentration [ug/ml]
2
6
4
0
0
2
Estimated Concentration [ug/ml]
8
Alexa488
0
1
2
3
4
5
6
7
8
9
0
1
2
True Concentration [ug/ml]
3
4
5
6
7
8
9
True Concentration [ug/ml]
, " 2.$""2.+&( / , 1 ' * . ' E Proc. of SPIE Vol. 7075 70750J-7
Alexa532
10
8
6
0
0
2
2
4
Estimated Concentration [ug/ml]
8
6
4
Estimated Concentration [ug/ml]
10
12
12
Alexa488
0
1
2
3
4
5
6
7
8
9
0
1
2
True Concentration [ug/ml]
3
4
5
6
7
8
9
True Concentration [ug/ml]
, * 2.$""2.+&( / , G ! !
! !)**(' !234(' FITC
0.8
0.6
0.4
Estimated Concentration [ug/ml]
15
10
0
0.0
0.2
5
Estimated Concentration [ug/ml]
20
1.0
25
1.2
Alexa488
0
1
2
3
4
5
6
7
8
9
0
0.05
0.1
True Concentration [ug/ml]
0.15
0.2
0.25
0.3
0.35
0.4
0.45
True Concentration [ug/ml]
, #) 2.$"" 8 5 / , 1 FITC
0.6
0.4
Estimated Concentration [ug/ml]
0.2
8
6
4
0.0
0
2
Estimated Concentration [ug/ml]
10
12
Alexa488
0
1
2
3
4
5
6
7
8
9
0
0.05
0.1
True Concentration [ug/ml]
0.15
0.2
0.25
0.3
0.35
0.4
0.45
True Concentration [ug/ml]
, ## 2.$"" 8 5 / , Proc. of SPIE Vol. 7075 70750J-8
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0.8
0.6
0.0
0
0.2
0.4
Estimated Concentration [ug/ml]
6
4
2
Estimated Concentration [ug/ml]
1.0
8
1.2
Alexa532
0
1
2
3
4
5
6
7
8
9
0
0.05
0.1
True Concentration [ug/ml]
0.15
0.2
0.25
0.3
0.35
0.4
0.45
True Concentration [ug/ml]
, #( 4 2.+&( 8 5 / , 1 FITC
0.6
0.4
Estimated Concentration [ug/ml]
0.2
5
0.0
0
Estimated Concentration [ug/ml]
10
0.8
Alexa532
0
1
2
3
4
5
6
7
8
9
0
0.05
0.1
True Concentration [ug/ml]
0.15
0.2
0.25
0.3
0.35
0.4
0.45
True Concentration [ug/ml]
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Proc. of SPIE Vol. 7075 70750J-9
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Proc. of SPIE Vol. 7075 70750J-10