Ce l l C u l t u re
g o es fully automated
Biomek
®
C e l l Wo r k s t a t ion
Cell Analysis
Centrifugation
Genomics
Proteomics
Lab Tools
Lab Automation
Particle Characterisation
Bioseparation
Simplify
Automate
Innovate
Biomek ®
Cell Workstation
An essential prerequisite in high throughput
and high content screening , stem cell research,
cell therapy, tissue engineering and production
of biopharmaceuticals is the permanent culturing
and production of cells.
Cell-based assays require cells of consistent quality
Feeding
Seeding
to reduce inter-assay variability.
Cell
Maintenance
Production cell lines must be optimized and
continuously monitored for consistent quality of
their products.
Counting
Maintaining living cells in continuous culture
and managing multiple cell lines with different
Passaging
Cell
Expansion
Harvesting
Counting
requirements in parallel is not an easy task.
Plating
Cell culture is a complex process consisting of
Cell
Production
multiple tasks including cell seeding, growing, harvesting, counting and passaging. Processes may
run for weeks or months and thus are demanding
specifically in terms of maintaining sterility
Pooling
Integrated Biomek® Workstations for research and
production have been developed to specifically
address automation of cell culture media optimization,
hybridoma selection, production cell line optimization
and fully automated monoclonal antibody production.
Biomek® Cell Workstation is the latest addition
to this range of cellular systems and was developed
in cooperation between Celisca and Beckman Coulter.
and tracking all relevant information.
Process to
Soluti o n
Software
• SAMI® Process Management
software to organize parallel
and interleaved processes.
Includes a calendar view to
simplify laboratory resource
management.
Biomek® Cell Workstation enables fully automated cell seeding,
• SAMI® Process Definition Editor
growth rate monitoring and quality control, cell harvesting and
enables users to graphically define
plating into microtiter plates.
complex processes consisting
Different cell lines can be cultured in parallel without user inter-
of multiple tasks.
vention over days, weeks or even months. Advanced software
control allows true 24/7 operation.
• Intuitive graphic editor to define
and schedule individual tasks in
Monolayer Cell Culture Process
10 ml PBS to
remove cell culture
medium/serum
cell culture flask,
confluent
monolayer of cell
10 ml buffer
10 ml cell
suspension
15 ml cell culture
medium per flask
0,5 ml cell suspension in new flasks
max. 20 new cell
culture flasks
(5x105 cells)
3 microplates,
96 wells,
30.000 cells/well
1 ml trypsin
EDTA
9 ml cell culture
medium (37°C)
150 l medium
per well
33 l cell suspension per well
0,5 ml cell
suspension
ce
via ll c
bil ou
ity nti
te ng,
st
incubation (3-5 days)
10ml
buffer
1 ml trypsin/EDTA on
cell monolayer to
detach cells
Incubation
(3-10 min, 37°C)
medium
detail. Integrated data tracking
9,5 ml cell
suspension (ca.
107 cells)
provides on-the-fly feasibility
checking of these tasks.
• Pipetting actions can be optimized for any cell type, liquid
class and and labware type.
• Unique integrated data management, i.e. tracking, logging and
reporting. Any information about
any sample, well, or plate is
registered in a database and
available for later use. Labware
reports provide information about
Biomek Cell Workstation is based on the Biomek liquid handler
every single plate in the system
and includes new hard- and software components to automate
at any point of time.
®
®
cell culture applications.
• Data management system
The system can be used with both adherent and suspension
includes SQL viewer for easy
cell cultures and can be easily optimized for different growth
LIMS connectivity.
characteristics. Advanced planning and scheduling tools allow
multiple tasks such as cell line expansion and media exchange
to occur simultaneously.
Process to
So lution
The software components of Biomek® Workstation enable users to run and monitor complex processes over weeks
and months. Biomek Workstation utilizes the Biomek® liquid handler’s integrated SQL data®
base to ensure integrity of all process steps. Beckman Coulter’s data acquisition and reporting tool is an integral
part of any Biomek® Workstation system. Data acquisition and reporting insures data integrity between tasks
and utilizes the integrated SQL database. These data management tools
allow tracking of locations for every single plate in the system at any
Easy to use...
point of time. SAMI® Process Management Software includes a view
of future, current and completed tasks within the system. This
interface provides easy access to more detailed reports, including
detailed labware reports.
At the same time, the software system provides flexibility for the user
to optimize each single step within a process and configure entirely
new processes.
... to flexible
Planning and Process Definition
and Optimization
SAMI ® Process Definition Editor is an intuitive graphical user interface to map tasks
within a complete process.
Any SAMI ® EX task is available as a building
block for new processes or process variations.
Task Definition and Optimization
Biomek ® Cell Workstation includes Beckman Coulter’s
SAMI ® EX optimizing scheduler to define and run
individual tasks in any cell cultivation process.
New tasks can be developed on a simulation system
without interfering with running processes.
Pipetting Optimization
Any pipetting action, e.g speed and
tip motion path can be optimized
for any type of labware and cell line.
More Options
The Biomek® Cell Workstation is a modular system
that can be tailored to your specific needs. Systems can range
from small footprint workstations for simple cell seeding and feeding to
multiple workstation configurations for complex processes such as fully automated monoclonal
antibody production.
• Integration of large capacity CO2 Incubators with optional customization for specific environmental
control needs
• Expanded liquid handling capability including multichannel pipetting for 96- or 384 well plates
• Integration of devices for a variety of cell growth characteristics
• Integration of high performance plate readers such as Beckman Coulter’s Paradigm™ Detection Platform
• Integration of Beckman Coulter‘s Flow Cytometry instruments for high content cell analysis
• Integration of analyzers for quality control of biopharmaceutical proteins
• Fully automated system for cell fusion in monoclonal antibody production
• Options for controlled rate cell freezing and media collection in cell line optimization systems
Biomek ® Cell
Works t a t i o n
Biomek® Cell Workstation is based upon Beckman Coulter’s
Biomek® Liquid Handler and provides modular integration of
components for fully automated cell culture in microplates
and automation friendly tissue culture flasks.
www.beckmancoulter.com/labautomat
3D TiltALP for culture flask handling
• Biomek® NXP for liquid handling and labware transport on
workstation deck provides small volume handling with high
accuracy and precision
TiltALP for multiwell plates
• Sterile enclosure with HEPA filtration and laminar flow
• Integrated CO2 incubator
• Barcode scanning for labware registration and confirmatory reads
Barcodes and other labware properties are tracked through the
entire process
• Integrated Vi-CELL® Cell Viability Analyzer generates cell count
189 slot CO2 incubator for cell growth
and viability data to normalize cell densities in assay plates
• Proprietary devices such as the 3D tilting-clamping-tapping ALP
• On-deck shaking incubator for trypsinization / cell dissociation
procedures
• Refrigerated multiple media storage with in-line warming
• Integrated high volume rapid bulk media dispense
dual slot shaking incubator
• Self refilling disposable reservoirs for on-deck media supply
• Media quality control and quality management
plate lift
tion
Cell Culture g o e s
ful ly automated
Part No.
Description
CWS 0011
Biomek® Cell Workstation
Biomek® Cell Workstation Specifications
Height
2.615 cm
Width
1.800 cm
Depth
1.260 cm
Power Requirements
50/60 Hz, 100-240 VAC
Sufficient to support 181.4 kg (400 lb)
B I O M E K ® P I P E T T I N G P E R F O R M A N C E S P E C I F I C AT I O N S
SPAN-8 SYSTEMS
Transfer
Span-8
Volume
Syringe Volume
0.5 µL
250 µL
1 µL
250, 500, 1000 µL
5 µL
250, 500, 1000 µL
10 µL
500 µL
50 µL
500 µL
100 µL
500 µL
250 µL
500 µL
950 µL
1000 µL
Tip
Accuracy
Types
±%
P20, Fixed 60 mm
5
P20, P50, Fixed 60 mm
3
P20, Fixed 60 mm
3
P50, P250
3
P250
3
P250, P1000
3
P1000
2
P1000
1
Precision
<%
10
7
5
5
5
5
2
1
M U LT I C H A N N E L S Y S T E M S
Transfer
Multichannel
Volume
Configuration
0.5 µL
384
1 µL
96 & 384
5 µL
96 & 384
Tip
Types
P30
P20, P50, P30
P20 & P30
Precision
<%
7
5
5
Accuracy
±%
3
3
3
Beckman Coulter and Celisca are able to provide further customization
of Biomek® Cell Workstation specific to your needs.
Please contact your Beckman Coulter representative to learn more about
automated cell culture systems, cellular analysis, quality control in
production of biopharmaceuticals or genetic analysis tools.
Our Solutions for your Automation!
Biomek® 3000
1- or 8-Channel Pipettor
Biomek® NXp
Span-8 or (96) 384-channel
pipetting Workstation
Biomek® FXp
2 independent pod-systems for
Span-8 or multichannel (96/384)
Czech Republic, Prague (420) 267 00 85 13
Eastern Europe, Middle East, North Africa, South West Asia: Switzerland, Nyon (41) 22 365 3707
France, Villepinte (33) 1 49 90 90 00 Germany, Krefeld (49) 2151 33 35
India, Mumbai (91) 22 3080 5101 Italy, Cassina de’ Pecchi, Milan (39) 02 953921
Netherlands, Mijdrecht (31) 297 230630 South Africa/Sub-Saharan Africa, Johannesburg (27) 11 805 2014/5
Spain, Madrid (34) 91 3836080 Sweden, Bromma (46) 8 564 85 900 Switzerland, Nyon (41) 0800 850 810
Turkey, Istanbul (90) 216 309 1900 UK, High Wycombe (44) 01494 441181
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