Proteinase K Quick Facts
General Info
Other Names
Protease Type
Molecular Weight
Activity
protease K, endopeptidase K, ProK
Serine protease of the subtilisin family
28.5 kDa
30 units/mg, 600 A U/ml in 20 mg/ml solution
Usage Info
Storage
Gram-Solution Conversion
Common Applications
Solubility
-20 °C up to 1 year
(20 mg/ml sln) 1 gram produces 50 ml
DNA & RNA isolation, genomic DNA isolation,
protein digestion
Dissolves in water, tris & PBS
Activation & Optimization
Optimal Temperature
Optimal pH
Activators of Proteinase K
Inactivation
Inactivation Temperature
Proteinase K Inhibitors
50 - 65 °C (active between 25 - 65 °C)
7.5-12
Ca2+, SDS, Urea
95 °C for 10 minutes
AEBSF (Pefabloc ®), PMSF, DIFP