AmpliTaq Gold® Fast PCR Master Mix,
UP (2X)
Insert P/N 4390945 REV A
Printed in USA
For Research Use Only.
Not for use in diagnostic procedures.
AmpliTaq Gold® Fast PCR Master Mix, UP (2X), 250 µl
AmpliTaq Gold® Fast PCR Master Mix, UP (2X), 2.5 ml
AmpliTaq Gold® Fast PCR Master Mix, UP (2X), 25 ml
P/N 4390937
P/N 4390939
P/N 4390941
AmpliTaq Gold® Fast PCR Master Mix, UP is designed to increase PCR amplification speed. The
amplification time (including ramping rate) is approximately 40 min for a 500 bp human genome DNA
fragment. The AmpliTaq Gold® Fast PCR Master Mix, UP has been optimized to generate template for DNA
sequencing reactions.
Read MSDS. For safety and biohazard guidelines, refer to the “Safety” section in the AmpliTaq Gold® Fast PCR
Master Mix, UP Protocol (PN 4391138).
This provides simplified procedures for using AmpliTaq Gold® Fast PCR Master Mix, UP. For details, refer to
AmpliTaq Gold® Fast PCR Master Mix, UP Protocol (PN 4391138).
Quick Guide Fast PCR Protocol
Reaction Components: The table below lists the components, volumes, and concentrations that comprise
the reaction mix.
Component
Volume per Reactiona
Final Conc
Deionized water
See belowb
User Provided Primer 1
User Provided Primer 2
User Provided template
AmpliTaq Gold® Fast PCR Master Mix, UP (2x)
Total volume
_µl
_µl
See belowb
10 µl
20 µl
0.2 µM- 1.0 µM
0.2 µM- 1.0 µM
<µg/reactionc
1X
-
a. Reaction volume may be adjusted to your experimental design, keeping the concentration of each component
constant.
b. Use any combination of water and template as long as the total volume of the PCR Master Mix, sample, and primers
equals 20 µl
c. Preferably > 100 copies of template but < 0.2 µg DNA per reaction
Step
Reactivation
of Enzyme
Hold
PCR
Final Step
Cycle (35 Cycles)
Hold
Hold
Denature
Annealing
Extension*
Temp
95 °C
96 °C
Primer Tm
68 °C
72 °C
4 °C
Time
10 min
3 sec
3 sec
See table below
10 sec
∞
Fast Thermal Cycling Profile: Program the Fast thermal cycler using a combination of the following
parameters for your specific target sequence.
Use Primer Tm calculator found on an Applied Biosystems Thermal Cycler
or go to www.appliedbiosystems.com/support/techtools/calc/

*Recommended extension times:
Length (Kb)
Extension Time
0.5
5 sec
1.0
15 sec
1.5
30 sec
© Copyright 2007, Applied Biosystems. All rights reserved.
Applied Biosystems is a registered trademark and AB (Design) and Applera are trademarks
of Applera Corporation or its subsidiaries in the US and/or certain other countries.
Product Insert
Oct 2007
For Research Use Only. Not for use in diagnostic procedures.
NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by one or more of the following US patents and corresponding
patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152 (claims 123 only) and 5,773,258 (claims 1 and 6 only), and claims outside the US corresponding to US
Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable
immunity from suit under the foregoing patent claims for using only this amount of product for
the purchaser’s own internal research. No right under any other patent claim (such as the
patented 5’ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972) and no right
to perform commercial services of any kind, including without limitation reporting the results of
purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by
implication, or by estoppel. This product is for research use only. Diagnostic uses require a
separate license from Roche. Further information on purchasing licenses may be obtained by
contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,
California 94404, USA.