International Journal of Systematic and Evolutionary Microbiology
Supplementary material
Sulfurirhabdus autotrophica sp. nov., isolated from a freshwater lake
Tomohiro Watanabe*, Hisaya Kojima, Arisa Shinohara, Manabu Fukui
The Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan
E-mail:
[email protected]
97
80
77
79
88
94
Sulfuricella denitrificans skB26T (AB506458 )
Sulfuricella sp. T08 (BBWF00000000)
‘Sideroxydans lithotrophicus’ ES-1 (CP001965)
Sulfurirhabdus autotrophica BiS0T (LC076475)
Sulfurisoma sediminicola BSN1T (AB842428 )
Sulfuritalea hydrogenivorans sk43HT (AB552844)
Sulfuriferula plumbophilus Gro7T (EF618604 )
Sulfuriferula multivorans TTNT (LC005595)
Thiobacillus denitrificans ATCC 25259 (CP000116 )
Thiobacillus thioparus DSM 505 (AJ294326)
Allochromatium vinosum DSM 180T (NC_013851)
0.05
Fig. S1. Phylogenetic position of strain BiS0T within the class Betaproteobacteria, based on SoxB amino
acid sequences aligned by ClustalW (117 amino acids were used). Allochromatium vinosum was used as an
outgroup. Tree was constructed by the neighbor-joining method with 1000 bootstrap resamplings
(bootstrap values > 50% are shown at the nodes). Bar, 0.05 substitutions per amino acid position.
97
Thiobacillus denitrificans ATCC 25259 (NC_007404)
Thiobacillus thioparus DSM 505T (EF641920)
79
Sulfuriferula plumbophilus Gro7T (EF641917 )
96
Sulfuriferula multivorans TTNT (LC005594)
Sulfurirhabdus autotrophica BiS0T (LC076473)
Sulfuricella denitrificans skB26T (AB506457 )
75
95
Sulfuricella sp. T08 (BBWF00000000)
Sulfuritalea hydrogenivorans sk43HT (AB552843 )
96
Sulfurisoma sediminicola BSN1T (AB842430 )
Allochromatium vinosum DSM180T (NC_013851)
0.05
Fig. S2. Phylogenetic position of strain BiS0T within the class Betaproteobacteria, based on AprA amino
acid sequences aligned by ClustalW (117 amino acids were used). Allochromatium vinosum was used as an
outgroup. Tree was constructed by the neighbor-joining method with 1000 bootstrap resamplings
(bootstrap values > 50% are shown at the nodes). Bar, 0.05 substitutions per amino acid position.
95
74
58
Thiobacillus denitrificans ATCC 25259 (CP000116 )
Thiobacillus thioparus DSM 505T (NZ_ARDU00000000)
Sulfurisoma sediminicola BSN1T (AB842431)
Sulfuritalea hydrogenivorans sk43HT (AP012547 )
Sulfuriferula multivorans TTNT (LC005597)
64
92
97
Sulfurirhabdus autotrophica BiS0T (LC076476)
Sulfuricella denitrificans skB26T (NC_022357)
Sulfuricella sp. T08 (BBWF00000000)
‘Sideroxydans lithotrophicus’ ES-1 (CP001965)
Thiomonas intermedia K12 (NC_014153)
Methylobacter tundripaludum SV96T (NZ_AEGW00000000)
0.05
Fig. S3. Phylogenetic position of strain BiS0T within the class Betaproteobacteria, based on Sqr amino
acid sequences aligned by ClustalW (118 amino acids were used). Methylobacter tundripaludum was used
as an outgroup. Tree was constructed by the neighbor-joining method with 1000 bootstrap resamplings
(bootstrap values > 50% are shown at the nodes). Bar, 0.05 substitutions per amino acid position.
100
89
69
78
93
Sulfurisoma sediminicola BSN1T (AB842429)
Sulfuritalea hydrogenivorans sk43HT (AP012547 )
‘Sideroxydans lithotrophicus’ ES-1 (CP001965)
Sulfuricella denitrificans skB26T (AP013066)
Sulfuriferula multivorans TTNT (LC005596)
Sulfurirhabdus autotrophica BiS0T (LC076474)
Thiobacillus thioparus DSM 505T (EU155054)
100
Thiobacillus denitrificans ATCC 25259 (CP000116 )
‘Thiorhodococcus drewsii AZ1’ (NZ_AFWT00000000)
0.1
Fig. S4. Phylogenetic position of strain BiS0T within the class Betaproteobacteria, based on DsrA amino
acid sequences aligned by ClustalW (84 amino acids were used). ‘Thiorhodococcus drewsii’ was used as
an outgroup. Tree was constructed by the neighbor-joining method with 1000 bootstrap resamplings
(bootstrap values > 50% are shown at the nodes). Bar, 0.1 substitutions per amino acid position.