Regulation of cyclins during the cell cycle
Replicative senescence
TGFβ
Replicative senescence
Hormones
UV stress
Cyclin H
p18INK4C
p16
INK4A
INK4
Family
p15INK4B
P
CDK4/6
P
P
Cyclin D
c-Jun
c-Fos
CDC25A
P
DP1
P
P
Cyclin B
CDK1
P
P
CDK1
P
P
P
Cyclin B
P
Cyclin H
CENP-F
APC/C
CDC20
Discover more at abcam.com/cellcycle P
CDK7
Spindle assembly
checkpoint extinguished
S phase
genes
P
Myt1
E2F
Proteasomal
degradation
Cyclin E
PLK1
APC/C
Aurora B
Cyclin A Cyclin B
Cyclin D
G1
S
G2 Mitosis
Phase
Cyclin B
Cyclin B
proteasomal degradation
Cyclin protein levels
Bim
CDK1
Ub
Ub
Ub
• Cyclin A
• Cyclin E
• CDK1
• DNA
• Polymerase α
• PCNA
• etc...
P
Beta-TRCP
P
P
P
Rb
DP1
P
Positive
feedback loop
Cyclin B
Increased cyclin B
protein levels
P
E2F
Myt1
Metaphase
plate alignment
P
Wee1
P P
CDK1
Cip/Kip Family
Inhibition of
transcription
Rb
Dephosphorylation
P
phosphorylation
Cyclin E
CDC25A/B/C
Wee1
P : Activating
Cip
P
Cyclin D
G2/M phase
p14ARF
Cyclin E
P
P
p21
or
CDK2
P
MAPKs
P
phosphorylation
p57Kip2
p27Kip1
Dephosphorylation
GSK3β
Mitogen withdrawal
Mitogens
P
P : Inhibitory
Cyclin-activating
kinase (CAK)
CDK7
p19INK4D
Key
p53
Differentiation
Protein levels
G1/S phase
Anaphase
progression
Produced in collaboration with David Bruce and Seán Mac Fhearraigh
Regulation of cyclins during the cell cycle
Clonality
Application
M
ICC/IF, IHC-P, IP, WB
M
ICC/IF, IHC-P, IP, WB
M
IHC-P, IP, WB
M
ICC/IF, IP, WB
M
ICC/IF, IHC-P, IP, WB
M
ICC/IF, IP, WB
M
Flow, ICC/IF, IHC-P, WB
M WB
M
Flow, WB
M
ICC/IF, IHC-P, IP, WB
M
Flow, ICC/IF, IHC-P, IP, WB
M
Flow, ICC/IF, IP, WB
M
Flow, ICC/IF, IHC-P, IP, WB
M
Flow, WB
M
Flow, ICC/IF, IP, WB
M
Flow, ICC/IF, IHC-P, WB
M
WB
M
Flow, ICC/IF, IHC-P, IP, WB
M
IHC-P, IP, WB
M
Flow, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, IP, WB
M
ICC/IF, IHC-P, IP, WB
M
Flow, ICC, IHC-P, IP, WB
M
Flow, ICC/IF, IHC-P, WB
M
ICC/IF, IP, WB
Host
Species reactivity
Rb
Hu
Rb
Hu
Rb
Hu
Rb
Hu
Rb
Hu, Ms, Rt
Rb
Hu, Ms, Rt
Rb
Hu
RbHu
Rb
Hu, Ms, Rt
Rb
Hu, Ms, Rt
Rb
Hu
Rb
Hu
Rb
Hu, Ms
Rb
Hu, Ms, Rt
Rb
Hu, Ms, Rt
Rb
Hu, Ms
Rb
Hu, Ms, Rt
Rb
Hu
Rb
Hu, Ms, Rt
Rb
Hu, Rt
Rb
Hu
Rb
Hu, Ms, Rt
Rb
Hu, Ms, Rt
Rb
Hu
Product code
ab45145
ab32158
ab156574
ab32051
ab32384
ab32147
ab108357
ab124712
ab134122
ab32137
ab32053
ab33911
ab92376
ab124678
ab179445
ab32391
ab134108
ab108349
ab109199
ab32034
ab32049
ab75974
ab138496
ab32015
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Product code
ab141344
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ProductDetails
Cell Cycle Assay Kit (Fluorometric - Green) Sample type: Adherent cells, suspension cells
Cell Cycle Assay Kit (Fluorometric - Red) Sample type: Adherent cells, suspension cells
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Sample type: Adherent cells, suspension cells
Cell Cycle (pCdk/pHH3/Actin) WB Cocktail
Tested in human and mouse
HeLa Cell Cycle Lysate Set: Control/Thymidine/Nocodazole
Tested in WB
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Sample type: Adherent cells, suspension cells
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p21 Human SimpleStepTM ELISA Kit Sample type: Serum, plasma, cell culture extracts, cell culture media, tissue lysate
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Key: Hu – Human, Ms – Mouse, Rb – Rabbit, Rt – Rat,
– Rabbit monoclonal antibody (RabMAb®),
Discover more at abcam.com/cellcycle
Product code
ab112116
ab112117
ab139412
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ab136811
ab151282
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ab171571
Immunohistochemical analysis of paraffin-embedded
human mantle cell lymphoma tissue using ab134175.
Cyclin D1 antibody [EPR2241]
(ab134175)
Cyclin D1 plays an essential role in the control of
the cell cycle at the G1/S transition.
Clonality
M
Application
ICC/IF, IHC/P, IP, WB
Host
Rb
Species reactivity
Hu, Ms, Rt
332_13_AH
Antibody
Aurora B antibody [EP1009Y] Bim [Y36] CDC25A (phospho S124) [EPR8888]
CDC25C (phospho S216) [E190] CDK1 [E161] CDK2 [E304] CDK4 [EPR4513] CDK7 [EP4324] c-Fos [EPR883(2)] c-Jun [E254] Cyclin B1 [Y106] Cyclin E [EP435E] Cyclin H [EPR3929] DP1 [EPR4959(2)] E2F [EPR3818(3)] GSK3beta [Y174] Myt1 [EPR1844(2)] CDKN2A/p16INK4a [EPR1473] p21 [EPR3993] p27Kip1 [Y236] p53 [Y5] p57Kip2 [EP2515Y] PLK1 [EPR2650] Rb (phospho T821) [E231] t
uc t
od igh
Pr ghl
hi
Related Antibodies
– MitoSciences®
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