Lonza Rockland, Inc.
www.lonza.com
[email protected]
Scientific support: 800-521-0390
Customer service: 800-638-8174
Document # 18335-0911-05
Rockland, ME 04841 USA
FlashGel™ dye and markers
Product information
FlashGel™ DNA marker 100 bp – 4 kb
FlashGel™ DNA marker 100 bp – 3 kb
FlashGel™ DNA marker 50 bp – 1.5 kb
FlashGel™ quantladder 100 bp – 1.5 kb
FlashGel™ RNA marker 0.5 kb – 9 kb
FlashGel™ loading dye
Cat.
No.
50473
Introduction
FlashGel™ markers, quantladder and loading dye were
designed to optimize performance of the FlashGel™
system.
57033
FlashGel™ DNA
marker
50 bp – 1.5 kb
57034
FlashGel™ DNA
marker
100 bp – 3 kb
50475
FlashGel™
quantladder
100 bp – 1.5 kb
FlashGel™ DNA markers and FlashGel™ quantladder
Storage: +4°C for up to 18 months from the date of
manufacture
FlashGel™ DNA markers provide accurate size
estimation of DNA fragments. The FlashGel™
quantladder enables both size and quantity estimation.
FlashGel™ DNA markers and the FlashGel™
quantladder are provided ready-to-load. (Do not
exceed 5 l per well load volume on FlashGel™
cassettes.)
Product
Contents:
FlashGel™ DNA
marker
100 bp – 4 kb
500 l Ready-to-load.
Sizes: 100/200/300/500/800/
1250/2000/4000 bp
100 apps
Recommended for 1.2%
FlashGel™ DNA cassettes
500 l Ready-to-load.
Sizes: 50/100/150/200/300/
500/800/1500 bp
100 apps
Recommended for 2.2%
FlashGel™ DNA cassettes
500 l Ready-to-load.
Sizes:
100/300/500/800/1500/3000 bp
100 apps
Recommended for double-tier
FlashGel™ DNA cassettes
250 l Ready-to-load.
Sizes (DNA Amount):
100 bp (3ng)/ 250 bp (7.5 ng)/
400 bp (15 ng)/ 800 bp (21 ng)/
1500 bp (30 ng)
50 apps
FlashGel™ RNA marker
Storage: -80° C for up to 24 months from the date of
manufacture
The FlashGel™ RNA marker consists of RNA transcripts
0.5 kb – 9 kb. Denature FlashGel™ RNA markers with
Formaldehyde sample Buffer (Lonza Cat. No. 50571) for
5 minutes at 65C. Do not exceed 5 µl per well on
FlashGel™ cassettes.
Cat.
No.
50577
1
Product
Contents:
FlashGel™
RNA marker
50 l
Sizes: 0.5/1.0/1.5/3.0/5.0/9.0 kb
Safety information
For details regarding product safety, see material safety
data sheet (MSDS); call Lonza scientific support +1
(800) 521-0390.
FlashGel™ loading dye
Storage: room temperature for up to 12 months from
the date of manufacture
FlashGel™ loading dye is sample buffer optimized for
DNA and native RNA samples run on the FlashGel™
system. FlashGel™ loading dye is provided in a 5X
concentration.
For best results, dilute DNA sample into 1X loading dye
such that a 5 l load contains 5 – 20 ng DNA per band.
For native RNA, combine RNA sample with FlashGel™
loading dye up to a 5 µl per well volume.
Do not exceed final load volume of 5 l per well on
FlashGel™ cassettes.
Cat.
No.
50462
Product
Contents:
FlashGel™
loading dye
5 x 1 ml vials, part 50463
5X concentration
50473
50475
– 4,000 bp
– 2,000 bp
– 1,250 bp
– 800 bp
– 500 bp
– 300 bp
– 200 bp
– 100 bp
FlashGel™ DNA marker
100 bp – 4 Kb
1.2%; 12+1
(Standard)
57033
– 1,500 bp
– 800 bp
– 500 bp
– 300 bp
– 200 bp
– 150 bp
– 100 bp
– 50 bp
– 1,500 bp (30 ng)
– 800 bp (21 ng)
– 400 bp (15 ng)
– 250 bp (7.5 ng)
– 100 bp (3 ng)
FlashGel™ quantladder
100 bp – 1.5 Kb
1.2%; 12+1
(Quantitation)
57034
FlashGel™ DNA marker
50 bp – 1.5 Kb
2.2%; 12+1
(High concentration)
50577
– 3,000 bp
– 1,500 bp
– 800 bp
– 500 bp
– 300 bp
– 100 bp
– 9.0 Kb
– 5.0 Kb
– 3.0 Kb
– 1.5 Kb
– 1.0 Kb
– 3,000 bp
– 1,500 bp
–
bp
– 800
500 bp
– 300 bp
– 100 bp
FlashGel™ DNA marker
100 bp – 3 Kb
1.2%; 16+1
(Double-tier)
– 0.5 Kb
FlashGel™ RNA marker
0.5 Kb – 9 Kb
1.2%; 12+1 & 16+1
(RNA)
For Research Use Only.
Not for use in Diagnostic Procedures.
FlashGel™ markers and FlashGel™ quantladders are
manufactured for Lonza.
All trademarks herein are marks of the Lonza Group or its
affiliates.
© 2011 Lonza Rockland, Inc.
All rights reserved
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