APPENDIX
1. BuCT Medium (Malcolm et al., 1985)
Components
Concentration (g/1)
CaCl2, H2O
0.1
MgSO4.7H2O
0.2
NH4NO3
0.1
FeNaEDTA
0.01
K2HPO4
1.0
NaH2PO4.2H2O
0.67
Sodium propionate
0.5
Casaminoacids
1.0
Tween 80
0.5
Agar
8.0
*Baker and Torrey
2.0ml
Micronutrient solution
pH
6.8
*Micronutrient solution (Baker and Torrey, 1979)
mg/1
H3BO3
1.5
ZnSO4.7H2O
1.5
MnSO4.H2O
4.5
Na2MoO4.2H20
0.25
CuSO4.5H2O
0.04
2. Qmod medium (Lalonde and Calvert, 1979)
Components
Concentration (per litre)
K2H PO4
0.3g
NaH2 PO4
0.2g
MgSO4 7H2O
0.2g
KCl
0.2g
Yeast extract
0.5g
Bactopeptone
5.0g
Glucose
10.0g
Ferric citrate (citric acid and ferric 1.0ml
citrate, 1% solution)
* Minor salts solution
1.0ml
** Lecithin solution
1.0ml
pH
6.8-7.0
* Minor salts solution
CoSO4. 7H2O
CUSO4. 5H2O
H3BO3
MnSO4. 7H2O
(NH4)6 Mo7O2. 4H2O
ZnSO4. 7H2O
0.01g
0.10g
1.50g
0.80g
0.20g
0.60g
** Lecithin solution
Lecithin
Ethanol
Distilled water
Made by dissolving 50 mg lecithin in
distilled water.
50mg
50ml
50ml
50ml of ethanol and adding 50ml of
3. DPM Medium (per litre for final solution) (Baker and O’Keefe,
1984)
Components
Concentration
KH2PO4
1.0g
MgSO4. 7H2O
0.10g
Cacl2. 2H2O
0.01g
Sodium propionate
1.20g
* Chelated iron solution
1.8ml
** Trace element solution
1.0ml
pH
6.8
* Chelated iron solution
FeSO4. 5H2O
5.54g
Disodium EDTA
7.56g
** Trace element solution (modified from Hoagland and Arnon, 1950)
CoCl2
0.025g
CuSO4. 5H2O
0.08g
H3BO3
2.86g
MnCl2. 4H2O
1.81g
NaM0O4. 2H2O
0.025g
ZnSO4. 7H2O
0.22g
4. Frankia Basal Medium (Murry et al., 1984)
Components
Concentration g/1
K2HPO4
3.0
KH2PO4
2.0
MgSO4. 7H2O
0.2
Nacl
0.3
FeNaEDTA
0.16
Casaminoacids
3.0g
Sodium pyruvate
3.0g
Trace element solution (Baker and 1ml
Torrey, 1979)
* Vitamin mixture
1ml
* Vitamin Mixture (Tjepkema et al., 1981)
Thiamine Hcl
Pyridoxine Hcl
Nicotinic acid
mg/100ml
10
50
50
5. Hoagland’s nutrient solution (Huss Danell and Myrold, 1994)
mg/l
CaSO4. 2H2O
86
Ca(H2PO4)2
109
MgSO4. 7H2O
123
FeSO4. 7H2O
6.95
Na2EDTA
9.3
H3BO3
0.715
MnCl2. 4H2O
0.453
ZnSO4. 7H2O
0.055
CuSO4. 5H2O
0.02
Na2 MoO4. 2H2O
0.0062
CoCl2. 6H2O
0.0062
pH
6.0
6. Jensen’s Medium
Components
Concentration g/1
Sucrose
20
K2HPO4
1.0
MgSO4. 7H2O
0.5
Nacl
0.5
FeSO4
0.1
CaCO3
2.0
Agar
Distilled water
pH
15.0
1 litre
7.0