A New Era in High Speed Analysis with
Dynamic MRM and UHPLC
Tim Schlabach, Marketing
Anabel Fandino, Ph.D., R&D
PeteStone, Ph.D., Marketing
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Agilent LC/MS, GC/MS, and ICP-MS Products
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ASMS
Training
Agilent LC/MS, GC/MS, and ICP-MS Products
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ASMS
Training
Agilent 1290 Infinity LC Attributes for MS
- Infinitely Better for LC/MS
High
Pressures
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1200bar @ 2mL/min for highest resolution
Reduced Ion & Matrix Suppression
Low
Delay Vol.
Less than 100 uL delay volumes
First peaks can elute in < 6 seconds
High
Throughput
HT-Solution for up to 2,000 samples/day
Complete System Integration MassHunter
Agilent 6000 Series LC/MS: Speed for UHPLC
Fast MRMs
for UHPLC
‰ 1 ms dwell for MRM
‰ 30 ms Pos/Neg Switching
‰ > 200 MRMs/ sec
Agilent 6460
Best
Data
Quality
Fast Scans
for UHPLC
Agilent 6530
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‰ 40 MS Scans/sec
‰ 10 MS/MS scans/sec
1290 Infinity LC means fast peaks, < 3 seconds
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Fast MRMs with 6400 Series QQQ – metabolism
polarity switching in 30 millisecs plus 6 transitions (2 ISTDs)
Avg W1/2 = 0.71 sec
15 points across
Verapamil,
0.75 min
RSD [%] = 2.9
Avg W1/2 = 1.3 sec
35 points across
Buspirone,
0.40 min
RSD [%] = 1.8
Dextromethorphan,
0.53 min
Avg W1/2 = 0.52 sec
11 points across
RSD [%] = 6.5
Avg W1/2 = 1.3 sec
35 points across
RSD [%] = 2.7
Diclofenac,
1.04 min
Peaks widths with UHPLC only 2 seconds or less
> ten uniform measurements across peak REQUIRED
Verapamil, peak RSD 5%
Avg. W1/2 = 0.72 sec
W = 2.1 sec
Cycle time < 115 ms
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Peak Capacities are very high with UHPLC
40 Extracted Ion Transitions in 1 minute
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Dynamic MRM for 6400 Series Triple Quads
monitors ion transitions only when compounds elute
Cycle Time:
Concurrent MRMs
Dwell Time:
With DMRM the number of concurrent MRMs are fewer than with Time Segments
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Analysis of 600 Pesticide DMRM Transitions in 15 min.
Agilent 1290 Infinity LC + 6460 Triple Quad
9 replicates analyzed at 200 pg level
Mean area %RSD = 3.2
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1290/6230 Accurate Mass TOF Pesticide Screening
250 pesticides, 50 pg on column
17 min.
3 min.
Most peaks 1-2 seconds wide!
1.5 min.
The Need for Speed in UHPLC/MS/MS
‰ Cycle Times (MRM):
<150 milliseconds
‰ Dwell Times (MRM or DMRM): <= 2 millliseconds
‰ Polarity Switching (MRM):
< 50 milliseconds
‰ Spectral scans:
at 5 Hz or faster
‰ PPM Mass Accuracy:
at 5 Hz
‰ 20 K Resolution:
at 5 Hz
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Agilent LC/MS can meet your most demanding
application needs.
The ultimate Qual
solution
1290 Infinity LC and 6530 Accurate Mass Q-TOF MS
The ultimate Quant
solution
1290 Infinity LC and 6460 Triple Quad MS
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