Chao (Tony) Yang
Supervisor: Tom Warkentin
Co-investigators: Dr. Jeff Schoenau
Dr. Axel Diederichsen
Dr. Rosalind bueckert
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Biological nitrogen fixation (BNF) capabilities
of legume crops has been documented for
years.
Field pea (Pisum sativum L.) is one of the
most popular grain legumes which accounted
for over 16% of pulse production worldwide.
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Evaluating collected pea mutants for their
growth and BNF capabilities in symbiosis with
different R. leguminosarum bv. viciae strains.
Provide useful information for further pea
breeding and inoculant development
programs
Becker Underwood Nodulator XL®
15N
Frisson P56 nod-
CDC Meadow
Novozymes & Becker Underwood
Frisson P88 (hyper+)
Rondo-nod3 (super+)
6
Dry shoot weight (g per plant)
5
4
3
2
1
0
control
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
Frisson P88 (hyper+)
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
CDC Meadow
Fig. 1 Differences of shoot dry weight among all treatments
Rondo-nod3 (super+)
0.8
0.7
Dry root weight (g per plant)
0.6
0.5
0.4
0.3
0.2
0.1
0
control R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
Frisson P88 (hyper+)
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
CDC Meadow
Fig. 2 Differences of root dry weight among all treatments
Rondo-nod3 (super+)
50
45
Nodules on tap root of each plant
40
35
30
25
20
15
10
5
0
control R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
Frisson P88 (hyper+)
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
CDC Meadow
Fig. 3 Differences of nodule number on tap roots of each plant
Rondo-nod3 (super+)
500
450
Nodules on lateral roots of each plant
400
350
300
250
200
150
100
50
0
control R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
Frisson P88 (hyper+)
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
CDC Meadow
Fig. 4 Differences of nodule number on lateral roots of each plant
Rondo-nod3 (super+)
700
Shoot fixed N (mg per g of dry shoot tissue)
600
500
400
300
200
100
0
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL® R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
Frisson P88 (hyper+)
CDC Meadow
R.101 R.105 R.108 R.153 R.154 R.155 R.168 XL®
Rondo-nod3 (super+)
Fig. 5 Fixed N in shoot tissues of three pea lines after inoculated with seven strains
Inoculant strains
Nodule numbers
on lateral roots
101
105
108
153
154
155
168
Nodulator
XL®
Frisson P88
Sym29
0.109 0.200
-0.404
0.318
0.887*
-0.836
0.393
0.068
CDC Meadow
0.296 0.030
-0.335
0.307
0.262
0.118
-0.646
-0.215
Rando-nod3 (fix+) 0.032 0.181
0.592
0.070
0.937*
0.158
-0.646
0.281
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Different inoculant strains can affect pea growth differently.
Different rhizobia strains’ colonization lead to significant
difference in nodulation on tap and lateral roots.
BNF of pea is influenced by both pea cultivars and inoculant
strains.
Rondo-nod3(fix+) showed better potential of BNF with the
inoculation of strains R.154, while Frisson P88 showed better
relationship with R.101.
R.154 showed highest N fixation efficiency for both Rondonod3(fix+) and Frisson P88.
80.00
Fixed N in tested pea plant (kg per ha)
70.00
60.00
50.00
40.00
30.00
20.00
10.00
0.00
CDC Dakota
CDC Meadow
Frisson, P88 Sym 29 Rondo-nod3(fix+)
Fig. 6 Fixed N in shoot tissues of four tested pea lines under the field condition
Rondo-nod3(fix+)
and Frisson P88 was crossed with two commercial cultivars
(As we have two donors and two recipients, there were
4 different combinations of crossing follow these steps below)
F1
F2 seeds were grown in pouches, plants with good
nodulation performance were transplanted into pots
BC1F1 was selfed
BC1F2 seeds are growing in greenhouse to yield BC1F3 seeds
Plants selected from
each F2 line were selfed
Plants selected are back crossing with
their original recipient parent
BC1F3
F3
BC1F1
F3 seeds are selfed again to yield F4 seeds
Greenhouse test
Field test
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Dr. Hossein Zakeri
Eldon Siemens
Brent Barlow
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Agriculture Development Fund (ADF)
Western Grains Research Fundation (WGRF)
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Novozymes
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