The Power of Electrophoresis
Carroll Education Foundation and
Southlake Style Magazine
Sponsor OUR GRANT !!
Soon Afterward Our New Equipment
Arrives!! We are ready to perform
DNA Electrophoresis
Students pour their own agarose gels.
Beginning the DNA
Digest:
1. Add samples of DNA to
micro-test tubes
2. Add the Restriction
Enzyme that will locate
palindromes (patterns) in
the DNA samples and cut
our DNA into fragments.
3. Spin the test tubes using
a micro-centrifuge to be
sure our small samples
are mixed well
4. Provide time for the
restriction enzymes to
work.
DNA Digests work best if we use the
Hot Water Bath!
• Place all DNA samples
into special floating
racks and incubate for
20 minutes. This gives
the restriction
enzyme time to digest
each DNA sample into
fragments.
Now load the DNA samples into the
agarose gels and fill the
electrophoresis chambers.
Place the gels into the Electrophoresis
chambers and turn on the power!
Waiting for DNA samples to separateSomething is definitely going on inside
this chamber!!
Taking measurements
and Creating a Standard Curve to
complete the analysis of the suspect DNA
The completed Gel
• One Standard and
Four suspect DNA
samples analyzed.
Now let’s determine
which suspect’s DNA
matches DNA left at
the scene of the crime!
COULD IT BE WE FOUND THE
GUILTY PERSON?
We had a lot of fun learning
how DNA electrophoresis
works…..
Thank You
THANK YOU
FOR SUPPORTING OUR EDUCATION!