ABASM Newsletter Allegheny Branch of the American Society for Microbiology JANUARY 2013 2012 Annual Meeting at Penn State-University Park The ABASM Annual Meeting was held on November 9-10 at the Days Inn in downtown State College with approximately 100 members in attendance. ABASM received both national and regional ASM funds to increase the number of attendees from West Virginia and eastern Pennsylvania and to host a microbial biofilms workshop, “Biofilms in Microbiology Education and the Teaching Laboratory.” The meeting was co-sponsored by the Eastern PA Branch of ASM, which also assisted with web-based publicity. VWR International representative Tom Rodgers provided a sustaining membership contribution. ASM Branch Lecturer, Dr. Ferran GarciaPichel (Arizona State University) presented two lectures that complemented the workshop, “Dealing with the Sun’s Worst Side: Biochemistry, Genetics and Ecology of Microbial Sunscreens,” and “Microbial Adaptations in Biological Soil Crusts, Earth’s Most Extensive Biofilms.” Dr. Garcia-Pichel also met with grad students. Invited faculty speakers included: Dr. Jeffrey Newman (Lycoming College), “Bioinformatics in Microbiology Education” Dr. Guixin (Susan) He (University of Massachusetts at Lowell) “Multidrug Resistance Mechanisms in Enterobacter cloacae” Dr. Douglas Reed (University of Pittsburgh) “Combating Tularemia: Evaluation of Live Attenuated Vaccine Candidates for Francisella tularensis in a Rabbit Model” Dr. Gregory Broussard (University of Pittsburgh) “Integration-Dependent Bacteriophage Immunity: Evolution of Genetic Switches” Dr. Corien Bakermans (Penn State-Altoona) “Molecular Diversity, Cold Adaptation and Metabolic Activity of Antarctic Bacteria” Dr. Steve Knabel (Penn State-University Park) “Evolution of Bacterial Epidemic Clones” Mark your calendars for next Annual Meeting: Nov. 1-2, 2013 Saint Vincent College, Latrobe, PA Contact Dr. Jennifer Koehl 2012 Microbial Biofilms Workshop a Success! Thanks to a grant from national ASM, the 2012 Annual Meeting at Penn StateUniversity Park included two hands-on teaching workshops on microbial biofilms. The active-learning workshops were conducted by Dr. John Lennox, PSUAltoona Professor Emeritus of Microbiology and coeditor of Biofilms: The Hypertextbook. Lennox was assisted by Dr. Kim Finer, Kent State-Stark Professor of Biological Sciences, and Colin Lennox, of EcoIslands, LLC. Forty microbiology faculty, postdocs, staff, and graduate students participated and received materials to make batch and flow-through reactors for use in their own teaching and research labs. Cultures of Chromobacterium violaceum, kindly provided by Dr. Bob McLean, Texas State University-San Marcos, were also given to attendees for demonstrating quorum-sensing compounds in biofilms. Special thanks go to Dr. McLean and the Penn State Department of Food Science for hosting the lecture and workshop. Bill Costerton Remembered at Biofilms Workshop During his opening lecture, John Lennox honored the memory of his mentor and collaborator, Dr. John William Costerton, the “Father of Biofilm Science,” who passed away on May 12, 2012. Dr. Bill Costerton studied biofilms for more than 40 years, serving as Director of the NSF-sponsored Center for Biofilm Engineering at Montana State University from 1992-2004. More recently, Dr. Costerton had moved to Pittsburgh in 2008 where he became Director of Biofilm Research at the Center for Genomic Sciences of the West Penn Allegheny Health System. Dr. Costerton’s research led to many breakthroughs in our understanding of the roles of biofilms in human disease and environmental remediation. He and his work will be remembered for years and years to come. John Lennox next to his homemade multi-well pate platform shaker during set-up of the Microbal Biofilms Workshop in the Food Microbiology Teaching Laboratory. The gentle swirling action of the platform shaker helps distribute cell-binding dyes for quantifying biofilms growing in plate wells. Student Award Winners at ABASM 2012 Meeting Congratulations go to the graduate and undergraduate students who gave 17 oral presentations and exhibited 24 posters at the Annual Meeting. All presenters will receive a one-year student membership to ASM. The following is a list of the students who won cash awards for placing in their respective categories: Graduate Oral Presentation Awards 1st place – KellyAnn Miller (West Virginia University) The Flagellar Hook Proteins of Borrelia burgdorferi Form a High Molecular Weight Complex. Advisors: Drs. Michael Miller, Milinda James, and Nyles Charon, Health Sciences Center. 2nd place—Becky McCauley (Penn State-University Park) Looking for Life in All the Wrong Places: Microbial Communities in Anoxic, Energy-Poor Environments. Advisor: Dr. Jenn Macalady, Dept of Geosciences Graduate Poster Exhibition Awards 1st place-Michael Gresock (Penn State-University Park) Death of the TonB Shuttle Hypothesis, Advisor: Dr. Kathleen Postle, Dept of Biochemistry and Molecular Biology 2nd place—Katherine Baldwin (Shippensburg University) Incidence of Bacterial Pathogens in Common Tick Populations (Ixodes and Dermacemtor), Advisors: Drs. Marcie Lehman and Richard Stewart, Dept of Biology Undergraduate Oral Presentation Awards 1st place – Emily Hyde and Holly Pier (Penn State Erie-The Behrend College) A Comparison of the Microfloras Found on House Wren and American Kestrel Eggs. Advisors: Drs. Beth Potter and Margaret Voss, School of Science. 2nd place – Emilee Shine, University of Pittsburgh Characterization of a Non-Canonical Tyrosine Integrase. Advisors and Mentors: Drs. Gregory Broussard, Graham Hatfull, and Bryce Lunt, Department of Biological Sciences 3rd place – Jordan Krebs (Lycoming College) Purification and Molecular Structure Determination of Flexirubin Pigments from Chryseobacterium. Advisor: Dr. Jeff Newman, Dept of Biology. 3 Undergraduate Poster Exhibition Awards Environmental 1st place –Steven Strutt (Juniata College) Metatranscriptomics of Beach Microbial Community Response to the Deepwater Horizon Spill, Advisor: Dr. Regina Lamendella, Dept of Biology 2nd place—Shailand Abbott and Samantha Gorman (Penn State-Altoona) Cold Adaptations of Bacteria Found in Pennsylvania Cold Traps, Advisor: Dr. Corien Bakermans, Division of Mathematics and Natural Sciences Medical Microbiology 1st place – Andrew Maul and Keiko Sing (Juniata College) Impact of Resistant Starch Diets on Gut Microbial Community Dynamics, Advisor: Dr. Regina Lamendella, Dept of Biology 2nd place – Mitchell Dunklebarger and Erin McClure (Juniata College) Shifts in the Gut Microbiota of Inflammatory Bowel Disease Patients in a Longitudinal Study, Advisor: Dr. Regina Lamendella, Dept of Biology Molecular Microbiology 1st place – Zachary Weisner (University of Pittsburgh at Greensburg) Utilizing E. coli to Express dsRNA to Inhibit Molecular Pathways in C. elegans, Advisor: Dr. Olivia Long, Dept of Biology 2nd place—Enoch Tse (University of Pittsburgh) Investigating Start and Extended Start-Associated Sequences in Mycobacteriophage Adephagia, Advisors: Drs. Gregory Broussard and Graham Hatful, Dept of Biological Sciences ABASM recognizes and thanks all its officers and the following volunteer judges Merrilee Anderson, Mt. Aloysius College Tracey Baumgarten, Penn State-University Park Alyssa Bumbaugh, Penn State-University Park Mike Engle, Mt. Aloysius College Yury Ivanov, Penn State-University Park Xiufen Li, Penn State-University Park Ann Marie Furdock, PA College of Technology Guixin (Susan) He-Univ of Massachusetts-Lowell 4 Paul Babitzke, Penn State-University Park David Boehm, California University of PA Gail Rowe, LaRoche College Jennifer Koehl, Saint Vincent College Beth Potter, Penn State-Erie Emily Stowe, Bucknell University Tom Danford, West Virginia Northern Community College 2012 International Attendees “Molecular Characterization of HIV-1 and Determination of Antiretroviral Drug Resistance Mutations Among Treatmentnaïve Patients in Jos, North Central Nigeria” In 2011 an ASM International Professorship was awarded to Mary Ann Bruns to teach a 10-week short course in soil and environmental microbiology at Ahmadu Bello University in Zaria, Nigeria. Bruns was able to return some of the hospitality shown to her in Nigeria by inviting Dr. Steve Olonitola, Chair of ABU’s Microbiology Department, to the ABASM meeting on learning that he was visiting the U.S. on a Fulbright at Virginia Tech. Interestingly, a conversation during the meeting between Dr. Olonitola and Dr. Doug Reed, University of Pittsburgh, may lead to an international collaboration on multi-drug-resistant M. tuberculosis. Other international attendees of the biofilm workshop were Dr. Mohamed Morsey, a Borlaug Fellow from Egypt visiting Dr. Catherine Cutter in Food Science; and Dr. Xiufen Li, Jiangnan University, China, visiting the Dept of Civil and Environmental Engineering at PSU. Dr. Olonitola’s two posters at ABASM: “Multiple Antibiotic Resistance and Extended Spectrum β-lactamase Production of Escherichia coli Isolated from Processed Meat - ‘Suya,’ in Zaria, Nigeria,” and Dr. Steve Olonitola, visiting Fulbright Fellow from Ahmadu Bello University in Zaria, Nigeria, observes Colin Lennox adjust microscope. Colin is viewing biofilm development in a flow-through reactor during workshop setup. 5 ABASM recognizes and thanks the following students for their contributions to the 2012 meeting PSU-ASM Student Chapter members were a tremendous help in making the workshop and meeting run smoothly—thanks go to Jim Rose, Kevin Thyme, Ryan Fine, Danielle Regplogle, Briana Grant, Kara Scherer, Henry Lupari, and Bobby Norgard. The Saturday Student Mentoring Session was graciously offered by Tara Kennedy, Duquesne University, and Michael Gresock, Penn State-University Park. Transportation of poster boards and easels was coordinated by Hasan Coban, PSU Department of Agricultural and Biological Engineering, support by which is gratefully acknowledged. Thanks also go to Claudia Rojas, PSU Department of Ecosystem Science and Management, and Xin Peng, PSU Intercollege Program in Ecology. Penn State-ASM Student Members Danielle Replogle and Briana Grant helped with set-up for the Microbial Biofilms Workshop in the Food Microbiology Teaching Lab. 6 ABASM supports 3 ASM Student Organizations Juniata College Faculty Advisors, Professors Jill Keeney and Regina Lamendella [email protected], [email protected] President Ryan Trexler, [email protected] Duquesne University Faculty Advisor Prof. Pete Castric, [email protected] President Tara Kennedy, [email protected] Penn State-University Park Faculty Advisor, Prof. Paul Babitzke, [email protected] President Jim Rose, [email protected] Meet the New ABASM Officers Official elections were held at the Fall ABASM meeting held at Penn State University on Nov. 10, 2012. The following individuals will assume or retain their positions as of July 1, 2013, and will hold these offices until the dates indicated in parentheses: President: Jennifer Koehl (June 2014) Dept. of Biology Saint Vincent College Latrobe, PA 15650 [email protected] President-Elect: Jeffrey Newman (June 2014) Dept of Biology Lycoming College Williamsport, PA 17701 [email protected] Secretary: Alyssa Bumbaugh (June 2014) Forensic Science Penn State—University Park University Park, PA 16802 [email protected] Treasurer: Nancy Trun (June 2014) Department of Biological Sciences Duquesne University Pittsburgh, PA [email protected] Councilor: Ann Marie Furdock (June 2014) Natural Science Department PA College of Technology Williamsport, PA 17701 [email protected] Alternate Councilor: Gail Rowe (June 2014) Dept of Biology LaRoche College Pittsburgh, PA [email protected] ABASM’s website http://archive.asm.org/branch/brAllegheny/home.htm 7 Letter from 2012 ABASM President A great example of an enriching interaction between our branches was the addition of a Penn State undergraduate’s oral presentation to the November ABASM program. Brigit McLaughlin, an undergraduate Biology major in PSU’s Eberly College of Science, learned about ABASM from an EPAASM member whom she’d met during a summer internship at The Perelman School of Medicine at the University of Pennsylvania. Without this connection, Brigit would not have learned about the opportunity to present at ABASM, nor would meeting attendees have learned about Brigit’s internship research on HIV. Over the last 10 years, the main role of the ABASM President has been to organize the annual meeting at his or her home institution. One of my major tasks last January was to apply for national and regional funds from ASM to enhance meeting activities. I am pleased to report that ABASM received a total of $3800, which enabled us to offer the microbial biofilms workshop and to expand our membership. As a result, the 2012 Annual Meeting hosted at least 100 members, compared to attendance of about 6070 in recent years. This year’s attendees included faculty and students from West Virginia University, University of Pittsburgh, Penn State-Berks, and Susquehanna University. ABASM would like to foster all the new connections made in 2012! Being a faculty member at Penn State’s “main campus” of University Park, I would like to see more microbiologists across the entire Penn State system participate in ABASM or EPAASM. Nine of PSU’s 23 branch campuses fall within ASM Region 3, where ABASM includes Pennsylvania zip codes 15000-16999, 17200-17299, and 17700-17799 (in addition to all of West Virginia). Sixteen PSU branch campuses (including Hershey Medical School) fall within Region 2, where EPAASM covers Pennsylvania zip codes 17000-17199, 17300-17699, and 17800-19699 (in addition to Delaware and parts of New Jersey.) It is probably most practical for members to choose a branch based on mission or meeting proximity than on geographic boundaries. In any case, increased participation in both branches will only strengthen microbiology across the Commonwealth of Pennsylvania. This year’s meeting also benefited from cosponsorship with the Eastern PA Branch of ASM (EPAASM) and the extra publicity through http://www.epaasm.org/). I would like to thank EPAASM President, Dr. Patrick Piggot of Temple University, and Dr. Irving Nachamkin, Professor and Director of the Division of Laboratory Medicine at The Perelman School, for facilitating this year’s meeting co-sponsorship. It is my hope that communications and interactions will continue between our branches, because I think each offers a distinct venue for professional development. For example, ABASM meets just once a year (in the fall), while EPAASM meets monthly. ABASM’s mission emphasizes mentoring of undergraduate and graduate students through sponsorship and judging of student presentations in molecular, medical, and environmental microbiology. ABASM’s annual meeting also provides an important enrichment and dialogue event for microbiology educators, many of whom are the “lone microbiologist” in their respective departments at widely dispersed institutions. In contrast, EPAASM has a stronger clinical research orientation and draws from a more geographically concentrated pool of clinicians and researchers as well as educators. It has been a pleasure serving you as President, and I wish all the best to incoming President Jennifer Koehl. I look forward to another great turnout at Saint Vincent College in Latrobe on Nov 1-2, 2013! Mary Ann Bruns, Associate Professor, Soil Microbiology, Dept of Ecosystem Science and Management, College of Agricultural Sciences, Penn State-University Park 8
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