Laboratoryy Animal Se
ervices Centter
University of Z
Zurich
Laboratory Aniimal Services Ce
enter (LASC)
Winterthurerstr
trasse 190
CH-8057 Züricch
Phone +41 44 635 54 51
FAX +41 44 6335 68 75
www.lasc.uzh..ch
BIOPS
SY SYST
TEM LAS
SC
Generral remarks




T
Two letters of
o the alphab
bet are assign
ned to each room
T
The biopsiess are numberred from 000
01 to 9999 pe
er room
O
Once 9999 has
h been rea
ached, the nu
umbering of the
t respectiv
ve room startts from 0001 again
T
The numbering is continu
uous, indepe
endently from
m the type of biopsy (ear or toe tip)
EAR b
biopsy scheme
Ear marrkings
 Potential earr markings are written in capital letterrs: L, R, L/R, LL, RR
Additional markings
m
are possible
p
in ccase re-biops
sies have to be
b taken (seee „Re-biopsies“)
 A
Animal ID
Consists of: room letters + biopsy nu mber + ear marking
m
lette
ers
 C
LEFT (L)
RIGHT (R)
LEFT
T/RIGHT (L/R))
LEF
FT/LEFT (LL)
RIGHT//RIGHT (RR)
are housed in
n T3 cages, the
t following additional markings
m
are possible: L/R
RR, LL/R and LL/RR.
If mice a
Example
e
Cage 806
664 from invesstigator John Smith
S
with 4 fe
emales is housed in room Y25H.
Y
The
letters “AB
B” have been assigned to ro
oom Y25H. Th
he last biopsy
y that has been
n taken in
the room was number 2513.
2

Mouse 1: 1 ea
ar punch left, biopsy
b
numbe
er 2514  anim
mal ID: AB251
14 L

Mouse 2: 1 ea
ar punch right,, biopsy numb
ber 2515  an
nimal ID: AB25
515 R

Mouse 3: 1 ea
ar punch left + 1 ear punch right, biopsy number
n
2516  animal ID
A
AB2516 L/R

Mouse 4: 2 ea
ar punches lefft, biopsy num ber 2517  animal
a
ID AB2517 LL
1/6
Laborato ry Animal Services
S
Cen
nter
University of Zurich
Laboratory A
Animal Services Center
C
(LASC)
Winterthurersstrasse 190
CH-8057 Zürrich
Phone +41 444 635 54 51
FAX +41 44 6635 68 75
www.lasc.uzhh.ch
TOE T
TIP biops
sy schem
me
Toe marrkings
 Potential toe
e markings: 01
0 to 99
- Right hin
nd limb: 1, 2, 3, 4, 5
- Left hind
d limb: 6, 7, 8,
8 9, 10
- Right forrelimb: 20, 30
0, 40, 50
- Left forelimb: 60, 70,, 80, 90
 T
The biopsy number
n
is eq
qual to the lasst two digits of the contin
nuous numbeer
 A maximum of 2 toe tips per mouse ccan be taken
n
Animal ID
 C
Consists of: room letter + biopsy num
mber ( last two digits co
orrespond to the toe mark
kings)
RIGHT
Example
e
665 from invesstigator John Smith
S
with 4 m
males is house
ed in room Y25H. The
Cage 806
letters “AB
B” have been assigned to ro
oom Y25H. Th
he last biopsy
y that has been
n taken in
the room was number 2517.
2

Mouse 1: toe tip 10 + toe tip
p 8, biopsy nu
umber 2518  animal ID: AB
B2518

Mouse 2: toe tip 10 + toe tip
p 9, biopsy nu
umber 2519  animal ID: AB
B2519

Mouse 3: toe tip 20, biopsy number 2520
0  animal ID AB2520

Mouse 4: toe tip 20 + toe tip
p 1, biopsy nu
umber 2521  animal ID AB
B2521
Seite 2/4
LE
EFT
Laborato ry Animal Services
S
Cen
nter
University of Zurich
Laboratory A
Animal Services Center
C
(LASC)
Winterthurersstrasse 190
CH-8057 Zürrich
Phone +41 444 635 54 51
FAX +41 44 6635 68 75
www.lasc.uzhh.ch
Labelling of tu
ubes and
d plastic bags




Each tube iss labelled with
h the room le
etter and biopsy number ( without eear marking letters in casse
tthere are ear punches) using
u
an alco
ohol resistantt marker.
T
The tubes arre grouped to
ogether per ccage, per strrain or per re
esearcher (deefined as uniits) and packked
into a small plastic
p
bag ( the „unit“ of packing depends on th
he facility annd its organiz
zation).
T
The label is printed per „unit“ (e.g. ca
age) and the sample num
mbers “from…
… to …” are written
w
on it
T
The small plastic bags off a license ho
older may be
e packed together in a biggger plastic bag
Example
es for ear and
d toe biopsies
s
The tube
es for ear biopsies are labelled as follows :

T
Tube with biop
psy of mouse 1: AB2514

T
Tube with biop
psy of mouse 2: AB2515

T
Tube with biop
psy of mouse 3: AB2516

T
Tube with biop
psy of mouse 4: AB2517
The tube
es for toe tip biopsies
b
are la
abelled as follo
ows:

T
Tube with biop
psy of mouse 1: AB2518

T
Tube with biop
psy of mouse 2: AB2519

T
Tube with biop
psy of mouse 3: AB2520

T
Tube with biop
psy of mouse 4: AB2521
The biop
psies will be pa
acked as follows:

B
Biopsies are grouped
g
together (e.g. per ccage / researc
cher / strain) in
n a small plasttic bag contain
ning a label with
tthe continuouss number „from … to …”

S
Small plastic bag
b from one license holderr may be pack
ked in one bigger plastic bagg
Ear biopsies
Seite 3/4
Toe tip biops ies
Laborato ry Animal Services
S
Cen
nter
University of Zurich
Laboratory A
Animal Services Center
C
(LASC)
Winterthurersstrasse 190
CH-8057 Zürrich
Phone +41 444 635 54 51
FAX +41 44 6635 68 75
www.lasc.uzhh.ch
Identification of breed
ding cage
es and biopsy wo
ork orde rs in iRA
ATS


If biopsies have
h
to be ta
aken from ea
ach litter: the researcher adds a rem
mark in the box “addition
nal
information” when setting
g up a new b
breeding worrk order (e.g. “ear” or “toee”)  the animal caretake
ers
w
will take bio
opsies from each litter until revoca
ation through researcheer  withou
ut any rema
ark,
b
biopsies will be taken only on the bassis of work orders
a needed on a sporad
dic basis on
nly: the researcher sendds a work orrder for biop
psy
If biopsies are
ssampling
Re-bio
opsies





Seite 4/4
Re-biopsies are only taken in agree
ement with the
t
LASC Veterinary
V
Seervices in so
ome cases the
t
a
approval of the
t Cantonall Veterinary O
Office has to
o be obtained
d
G
Generally, re
e-markings are
a done by e
ear biopsy
T
The animal ID has to be updated
T
The re-biopssies have to be
b taken in a way that the animal stays identifiablle at all times
s
 Mouse is already marrked by ear b
biopsy: for in
nstance the mouse
m
alrea dy has one marking on the
t
rright side, an
nd in the cag
ge there are mice with markings
m
R, R/L,
R and RR . Consequen
ntly, the mou
use
has to be ne
ewly marked with two earr biopsies on the left side (Animal ID: AB3541 LL/R)
 Mouse is already marked by toe tip biopsy: an
a additional ear biopsy has to be ta
aken (only affter
w
weaning)
r
it turns out that a re-biopsy is difficult to be taken, aand the identification of the
t
If for some reason
has to be rep
a
animal is no longer guara
anteed, this h
ported to the LASC veterrinary service
es.