Biochem. J. (2013) 454, 543–549 (Printed in Great Britain)
doi:10.1042/BJ20121771
SUPPLEMENTARY ONLINE DATA
*School of Chemistry and Molecular Biosciences, Australian Infectious Diseases Research Centre, University of Queensland, St Lucia 4072, QLD, Australia, and †Institute for Molecular
Bioscience, St Lucia 4072, QLD, Australia
Figure S3 Impact of SL1344 siderophore supplementation on the H2 O2
sensitivity of SL1344, SL1344 ESR , SL1344 ES and SL1344 ES ESR
Bacteria plated were supplemented with either the siderophores produced by wild-type SL1344
(A) or the supernatant of a mutant SL1344 ES culture (B). H2 O2 (5 μl of 9.98 M) was added
to the centre of each plate and pictures were taken after an overnight incubation at 37 ◦ C. The
images presented are representative of results from three independent experiments.
Figure S1 Impact of the catecholate siderophores and monomeric catechol
supplementation on the H2 O2 sensitivity of SL1344, SL1344 ES and SL1344
ES*
Bacteria plated were supplemented with salmochelin and enterobactin produced by SL1344. For
supplementation with 2,3-DHB, the monomeric catechol was mixed with the agar medium prior
to plating the bacteria. H2 O2 (5 μl of 9.98 M) was added to the centre of each plate and pictures
were taken after an overnight incubation at 37 ◦ C. The images presented are representative of
results from three independent experiments.
Figure S2 H2 O2 sensitivity of strains with (A) or without (B) the ability to
import siderophores
The indicated bacteria were plated on to MM9 glycerol agar supplemented with 50 μM DIP.
H2 O2 (5 μl of 9.98 M) was added to the centre of each plate and pictures were taken after an
overnight incubation at 37 ◦ C. The images presented are representative of results from five
independent experiments.
1
Figure S4 Impact of catecholate and non-catecholate siderophore
supplementation on the H2 O2 sensitivity of SL1344 and SL1344 ES
Bacteria plated were supplemented with the indicated siderophore(s) produced by the E. coli
83972 siderophore biosynthetic mutants mentioned in brackets. H2 O2 (5 μl of 9.98 M) was
added to the centre of each plate and pictures were taken after an overnight incubation at 37 ◦ C.
The images presented are representative of results from three independent experiments.
Correspondence may be addressed to either of these authors (email [email protected] or [email protected]).
c The Authors Journal compilation c 2013 Biochemical Society
Biochemical Journal
Maud E. S. ACHARD*, Kaiwen W. CHEN*, Matthew J. SWEET†, Rebecca E. WATTS*, Kate SCHRODER†, Mark A. SCHEMBRI*1
and Alastair G. MCEWAN*1
www.biochemj.org
An antioxidant role for catecholate siderophores in Salmonella
M. E. S. Achard and others
Table S1
List of primers used in the present study
Primer
Gene deletion primers
fepA5 F
fepA5 R
fepA3 F
fepA3 R
iroN5 *
iroN3 F
iroN3 R
entC_Fwup
entE_Rvup
entE_Fwdn
entE_Rvdn
Screening primers
fepAF
fepAR
iroNF
iroNR
entC_Fwsc
entE_Rvsc
Sequence (5 →3 )
cggaattctagggataacagggtaatcggtaggaataaaacagtagctg
gaagcagctccagcctacacaggaatgaatcttcttgttcattg
gaagcagctccagcctacacaggaatgaatcttcttgttcattg
gaactaaggaggatattcatatggagcattaatacccatttctga
atgagagttaagaagttcatctggttaataaccgtggtttctacaggggtgtg
taggctggagctgcttc
gaactaaggaggatattcatatggcttattatgccggtgtgac
gcgaattctagggataacagggtaataaggaataaccatcaactccga
gcctttgctacgtcgctc
gaagcagctccagcctacacacaacgggtgaaaggtatacg
gaactaaggaggatattcatatgctgaaggagaaagagagatgg
acgtccggtatcttttaacatc
cttcacgcagcacgttc
ctctctttggcggagaaag
ccccttcccggatttactg
cagtgaaaataacccgcgt
caaaatgagatccggcattt
ccccgccacatagttcagc
*The 5 end for iron::kan was only 50 bp
Received 26 November 2012/17 June 2013; accepted 28 June 2013
Published as BJ Immediate Publication 28 June 2013, doi:10.1042/BJ20121771
c The Authors Journal compilation c 2013 Biochemical Society