Technical Note
TD015EN Ver1.2
Cellufine Phenyl
Cellufine Phenyl can be used
at high flow rates.
100
75
200
150
50
100
25
--
250
The flow/pressure curve for a Cellufine Phenyl
column confirms operating flow rates above 200
cm/h can be obtained. The compressibility of
Cellufine Phenyl is approximately 24%.
Bed height [ % ]
Linear flow rate [ cm/hr ] ‐ ○ ‐
300
50
0
0
0.02
0.04
0.06
0.08
Pressure [ MPa ]
0.1
0
0.12
Fig1.Pressure-flow rate curve for Cellufine Phenyl.
Column : I.D.9cm-40cm bed height / Mobile phase [water]
Cellufine Phenyl is chemically
stable
Relative capacity, BSA [ % ]
100
90
When stored in 0.5M NaOH for 180 days at 20℃, the
adsorption capacity of BSA remained stable.
When stored 0.2M HCl for 180 days at 20℃, adsorption
of BSA only decreased slightly.
80
0.2M NaOH/4℃
0.5M NaOH/4℃
70
0.2M HCl/4℃
0.2M NaOH/２０℃
60
0.5M NaOH/２０℃
The graph displays Capacity relative to the Adsorption
Capacity before storage (as 100%).
0.2M HCl/２０℃
50
0
30
60
90
day
120
150
180
Fig2.Stability test in alkali and acid at 4℃/20℃.
Relative capacity, BSA [ % ]
100
Cellufine Phenyl performance
remains constant over at
least 100 operating cycles.
75
0.2M NaOH
0.5M NaOH
50
0
20
40
60
cycle
TD015EN Ver1.2 (April 1, 2011)
80
Fig3. Stability after repeated cleaning (CIP) with NaOH.
Column : I.D.(4.4cm)
Flow :1.5CV/h [residence time 40min] at 20℃
100
Equilibration buffer: 0.01M sodium phosphate ,
pH7.0 + 1M (NH4)2SO4
Washing solution: 0.2M NaOH or 0.5M NaOH
Cycle: 1) Equilibration buffer 3CV; 2)Washing solution 3CV.
The Cellufine Phenyl was sampled at 50 cycles and 100 cycles,
and the adsorption capacity was determined.
Technical Note
TD015EN Ver1.2
Relative capacity, BSA [ % ]
100
Cellufine Phenyl can be
repeatedly autoclaved.
95
90
Autoclaving is recommended in a neutral,
low salt buffer, or water.
85
water
0.15M NaCl
80
0.01M PB,pH7 +0.15M NaCl
75
0
1
2
3
Autoclave cycle
4
5
Fig4. Change of adsorption capacity for Cellufine Phenyl
during autoclaving cycle.
Conditions: 20 minutes at 121℃
Cellufine Phenyl conforms to USP28, Plastic Class V.
2-1, Otemachi 2-chome, Chiyoda-ku
Tokyo 100-8105 Japan
[email protected]
TD015EN Ver1.2 (April 1, 2011)