For life science research only.
Not for use in diagnostic procedures.
Print
DNA Molecular Weight Marker VI (0.15 – 2.1 kbp)
pBR 328 DNA × Bgl I and pBR328 DNA × Hinf I digested
Cat. No. 11 062 590 001
1.
50 ␮g Ⳏ 1 A260 unit
50 ␮g (200 ␮l) for 50 gel lanes
y Version 07
Content version: May 2008
Store at ⫺15 to ⫺25°C
Product Overview
Content
Ready-to-use solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Concentration
250 μg/ml
Size Distribution
Fragment mixture of pBR328 DNA cleaved with restriction endonuclease Bgl I and pBR328 DNA cleaved with Hinf I.
As determined by computer analysis of the pBR328 sequence the mixture contains altogether 15 DNA-fragments with the following base
pair lengths (1 base pair 660 daltons) occurring once: 2176, 1766,
1230, 1033, 653, 517, 453, 394, 220, and fragments 298, 234, and 154
occurring twice as a result from digestion of pBR328 with both Bgl I
and Hinf I.
Application
Molecular weight marker for the size determination of DNA in agarose
gels.
Typical Analysis
The DNA fragment mixture shows the typical pattern of 11 bands in
agarose gel electrophoresis, as shown in the figure, with fragments
298, 234 and 154 occurring twice, resulting in one distinct band.
Separation Conditions
Apply 1 ␮g DNA per lane on a 2% Agarose MP* gel.
Fig. 1: Separation of 1 ␮g DNA Molecular Weight Marker VI on a
2% agarose gel. Ethidiumbromide stain.
2.
Storage/Stability
The unopened vial is stable at ⫺15 to ⫺25°C until the expiration date
printed on the label.
 This product is shipped on dry ice.
Store at ⫺15 to ⫺25°C until use.
Once thawed we recommend further storage at +2 to + 8°C.
 Avoid repeated thawing and freezing.
Supplementary Information
Changes to Previous Version
Regulatory Disclaimer updated.
Text Conventions
To make information consistent and understandable, the following text
conventions are used in this Instruction Manual:
Text Convention
Use
Asterisk *
Denotes a product available from Roche
Applied Science.
Symbols
Symbols are used in this Instruction Manual to highlight important
information:
Symbol Description

0212.03664155001➆
Information Note:
Additional information about the current topic or procedure.
www.roche-applied-science.com
Ordering Information
Roche Applied Science offers a large selection of reagents and systems for life science research. For a complete overview of related
products and manuals, please visit and bookmark our home page,
www.roche-applied-science.com,
and our Special Interest Sites, including “Mapping & Cloning”:
http://www.restriction-enzymes.com.
The convenient RE Finder Program located on our Bench Mate website, http://www.roche-applied-science.com/benchmate helps
you identify the enzymes that will cut your DNA sequence, and displays the names and recognition sequences of enzymes and isoschizomers as well as links to detailed information (e.g., package insert)
of the selected restriction enzyme.
Product
Restriction
Enzymes
Rapid DNA
Ligation Kit
Application
Packsize
Cat. No.
DNA restriction
Please refer to website or catadigestion
logue
11 635 379 001
Ligation of sticky- Kit (40 DNA
end or blunt-end ligations)
DNA fragments in
just 5 min at 15 –
25°C.
T4 DNA Ligase Ligation of sticky- 100 U
10 481 220 001
and blunt ended
500 units
10 716 359 001
DNA fragments.
(1 U/␮l)
rAPid Alkaline
1,000 U
04 898 133 001
phosphatase
11 097 075 001
Alkaline Phos- Dephosphorylation 1,000 U
(20 U/␮l)
phatase (AP), of 5´-phosphate
special quality residues from
for molecular nucleic acids.
biology
100 g
11 388 983 001
Agarose MP Multipurpose
11 388 991 001
agarose for analyti- 500 g
cal and preparative
electrophoresis of
nucleic acids
Agarose Gel For the elution of 1 Kit (max. 100 11 696 505 001
reactions)
DNA Extrac- DNA fragments
from agarose gels.
tion Kit
High Pure PCR Purification of PCR 50 purifications 11 732 668 001
Product Purifi- or enzymatic modifi- 250 purifications 11 732 676 001
cation Kit
cation reaction (e.g.
restriction digest)
SuRE/Cut Buf- Incubation buffers 1 ml each (10× 11 082 035 001
A,B,L,M and H for conc. solutions)
fer Set for
restriction enzymes
Restriction
Enzymes
SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 959 001
fer A
incubation
conc. solution)
SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 967 001
fer B
incubation
conc. solution)
SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 991 001
fer H
incubation
conc. solution)
SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 975 001
fer L
incubation
conc. solution)
SuRE/Cut Buf- Restriction enzyme 5 × 1 ml (10× 11 417 983 001
fer M
incubation
conc. solution)
03 315 843 001
Water, PCR
Specially purified, 100 ml
(4 vials of 25 ml)
Grade
double-distilled,
03 315 932 001
deionized, and auto-25 ml
(25 vials of 1 ml)
claved
25 ml
03 315 959 001
(1 vial of 25 ml)
20 mg (1 ml)
10 711 454 001
BSA, special Maintaining
enzyme stability
quality for
molecular
biology
Printed Materials
You can view the following manuals on our website:
Laminated Buffer Chart
Lab FAQS “Find a Quick Solution”
Restriction Enzyme FAQS and Ordering Guide
Molecular Weight Markers for Nucleic Acids
Poster “Rec. Sequences of Restriction Enzymes”
Trademarks
All product names and trademarks are the property of their respective
owners.
Regulatory Disclaimer
For life science research only. Not for use in diagnostic procedures.
Contact and Support
To ask questions, solve problems, suggest enhancements or report new
applications, please visit our Online Technical Support Site at:
www.roche-applied-science.com/support
To call, write, fax, or email us, visit the Roche Applied Science home page,
www.roche-applied-science.com, and select your home country. Countryspecific contact information will be displayed. Use the Product Search function to find Pack Inserts and Material Safety Data Sheets.
Roche Diagnostics GmbH
Roche Applied Science
68298 Mannheim
Germany