Tissue Identification
Exercise 6A/6 (begins page 48 in 8th edition, page 67 in 9th, 10th, 11th and 12th editions)
Lab 4 Objectives
Read lab Exercise 6A/6
Do Activities 1-4
Histology: identify the following tissue types using the complete name:
1. Simple squamous epithelium
2. Simple cuboidal epithelium
3. Simple columnar epithelium
4. Pseudostratified columnar epithelium (ciliated)
5. Stratified squamous epithelium non-keratinized (mucosa)
6. Stratified squamous epithelium keratinized (epidermis) (see figures 7.2 a & 7.6b)
7. Transitional epithelium
8. Areolar connective tissue
9. Adipose tissue
10. Reticular connective tissue
11. Dense regular connective tissue
12. Dense irregular connective tissue
13. Elastic connective tissue (not in 8th or 9th editions- see page 2 of this handout)
14. Hyaline cartilage
15. Elastic cartilage
16. Fibrocartilage
17. Compact bone / Osseous tissue
18. Blood
19. Skeletal muscle
20. Cardiac muscle
21. Smooth muscle
22. Nervous tissue
More pictures can be found in the Histology sections on the PhysioEx CD, or on the web at
http://www2.sunysuffolk.edu/czuraa
Optional Computer Activity:
PhysioEx Exercise 6B (On the PhysioEx CD-ROM packaged with the lab book)
pages P-15 to P-16 (back of the book) in 8th edition
pages PEx-21 to PEx-22 (back of the book) in 9th and10th editions
(not in 11th or 12th editions)
For Study:
Review Sheet Exercise 6A/6 pages 533-540 in 8th edition
pages 85-90 in 9th and 10th editions
pages 87-92 in 11th and 12th editions
Answers in the Instructors Manual at the Eastern Campus Library on reserve
Amy Warenda Czura, Ph.D.
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SCCC BIO130 Lab 4 Tissues
Amy Warenda Czura, Ph.D.
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SCCC BIO130 Lab 4 Tissues
Helpful Hints On Finding Tissues on Slides:
Keep in mind that the slides we have are all tissue/organ biopsies/sections and thus have the
potential to contain many tissue types on the same small piece of organ or tissue. You need to be able
to distinguish the defining characteristics of each type of tissue, not the name of the slide it comes from
or the whole biopsy. Regardless of source, once you understand what each type of tissue looks like
you will be able to pick it out no matter where it is located. Below is a list of the tissues with slides
you would be able to see that tissue type on relatively easily.
Tissue Type
Simple squamous epithelium
Simple cuboidal epithelium
Simple columnar epithelium
Pseudostratified columnar epithelium (ciliated)
Stratified squamous epithelium non-keratinized
(mucosa)
Stratified squamous epithelium keratinized
(epidermis)
Transitional epithelium
Areolar connective tissue
Adipose tissue
Reticular connective tissue
Dense regular connective tissue
Amy Warenda Czura, Ph.D.
Slide Label and Description
Lung and Bronchi (section of lung with alveoli
cut in cross section displaying walls composed of
simple squamous epithelium)
Simple Cuboidal Epithelium: Mammal Kidney
(cross section of kidney which contains tubules
made of simple cuboidal epithelium cut mostly in
cross section)
Human Simple Columnar Epithelium:
Gallbladder (cross section of the gallbladder with
simple columnar epithelium on luminal surfaces,
also has good smooth muscle)
Trachea l.s. (trachea cut longitudinally displaying
pseudostratified columnar epithelium with visible
cilia along luminal surface, also has good hyaline
cartilage)
Esophagus c.s. (cross section of esophagus
displaying stratified squamous epithelium on
luminal surface, also has smooth muscle, adipose,
and dense irregular CT)
Keratinized Stratified Squamous Epithelium
(cross section of hairless human skin with
stratified squamous epithelium on surface, also
has good dense regular CT)
Urinary Bladder Collapsed c.s. (section of
bladder wall, relaxed, with transitional epithelium
on luminal surface, also has good smooth muscle)
Areolar Tissue Spread Film (section of areola
composed entirely of areolar CT)
Sole of Foot (Human) sec. (cross section of heel
displaying adipose in dermal layer, also has very
good dense irregular CT and keratinized stratified
squamous epithelium)
Reticular Tissue sec. (cross section of a lymph
node: reticular CT forms the stroma of the node,
also has adipose)
Tendon White Fibrous Connective Tissue l.s.
(longitudinal section of a tendon composed of
dense regular CT, also has good dense irregular
CT and skeletal muscle
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SCCC BIO130 Lab 4 Tissues
Dense irregular connective tissue
Elastic connective tissue
Hyaline cartilage
Elastic cartilage
Fibrocartilage
Compact bone / Osseous tissue
Blood
Skeletal muscle
Cardiac muscle
Smooth muscle
Nervous tissue
Human Scalp sec. With Hair (cross section
through scalp skin displaying dense irregular CT
in the dermal layer, also has good keratinized
stratified squamous epithelium)
Aortic Elastic Tissue Human (longitudinal section
of aorta, with the bulk of wall composed of
elastic CT)
Trachea c.s. (cross section through trachea
showing C-shaped rings of hyaline cartilage, also
has good adipose, pseudostratified ciliated
columnar epithelium, and simple cuboidal
epithelium
Elastic Cartilage: Epiglottis sec. (longitudinal
section of epiglottis displaying elastic cartilage in
the center, also has good non-keratinized
stratified squamous epithelium)
White Fibro-cartilage: Pubic Symphysis sec.
(cross section of the junction of the pubic bones,
the pubic symphysis, composed of fibrocartilage,
also has skeletal muscle and adipose)
Bone Dry Ground, Human c.s. (section of
compact bone)
Human Blood Smear Wright Stain (stained
human blood smear)
Skeletal Muscle Teased (skeletal muscle cells
pulled apart, not sectioned, appear 3 dimensional)
Intercalated Discs, Heart sec. (cross section of the
heart wall showing all cardiac muscle cells)
Duodenum c.s. (cross section of small intestine
displaying smooth muscle deep to the epithelium,
also has good columnar epithelium)
Giant Multipolar Neurons Smear (smear of
central nervous system tissue showing large starshaped neurons)
Photo pages can be found in front of each slide tray indicating the various tissue types that are visible
on each slide. It would be very helpful for you to orient yourself one slide at a time using these photos
as a guide. Tissue types are generally best observed at 400X so that the cellular details that distinguish
them are evident. (The photos are available on the web at www2.sunysuffolk.edu/czuraa)
The easiest slides to start with, since they have only one tissue type on them are:
Areolar Connective Tissue
Blood
Bone
Cardiac Muscle
Skeletal Muscle
Giant Multipolar Neuron
Amy Warenda Czura, Ph.D.
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SCCC BIO130 Lab 4 Tissues